Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F45 I0 R1 24 50.5 1802666 89.0% 1604372 146.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_bme_pgi 3,486,574 C→A 100% A152E (GCA→GAA)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,486,5740CA100.0% 145.6 / NA 47A152E (GCA→GAA) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/26);  total (21/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCG  >  NC_000913_3_bme_pgi/3486430‑3486714
                                                                                                                                                |                                                                                                                                            
gCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaa                                                                                                                                          >  1:816164/1‑149 (MQ=255)
  cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaaca                                                                                                                                        >  1:686649/1‑149 (MQ=255)
    aTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTTAGAGAAAGTGTGGAACCTGGCGTTTCTCGAGGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaacaac                                                                                                                                      <  1:168279/149‑1 (MQ=255)
    aTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaacaac                                                                                                                                      >  1:660069/1‑149 (MQ=255)
            aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGCGAAAGTGGGCAACCTGTCGTTCCTCGACGTGACGGGCTGCATTGCACAGACTCTGCTTAATCTGGAAAAACAACCAGACGCt                                                                                                                              >  1:378036/1‑149 (MQ=255)
             ggTAAACCCGGACATTCTTATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTa                                                                                                                             <  1:868259/149‑1 (MQ=255)
              gTAAACCCGGAGATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTAt                                                                                                                            <  1:664353/149‑1 (MQ=255)
                    ccGGACATTCTGATGCGTTTGTTTGCACAGATGGCGCGTCGTCTGCAAGTCTTTTTAGAGAAAGTGGGCAACTTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCa                                                                                                                      <  1:319908/149‑1 (MQ=255)
                           ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGAc                                                                                                               >  1:851826/1‑149 (MQ=255)
                           ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAAACACTATAACTCAccccgcc                                                                                                               >  2:216254/1‑145 (MQ=255)
                               ggtgggTTTGGCTGTAGAGATGGCGCGTCGTCTGGAAGTCACGTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGATGTTAGGGGCCGGATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGTTATGACTCACCCGGACGGTa                                                                                                           <  2:727764/144‑1 (MQ=255)
                                     tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaa                                                                                                                                          >  1:810814/1‑112 (MQ=255)
                                     tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaa                                                                                                                                          <  2:810814/112‑1 (MQ=255)
                                     tttGTCTGCACAGATGGCGCGCCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaaa                                                                                                     <  2:606771/149‑1 (MQ=255)
                                      ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAAt                                                                                                    <  1:293279/149‑1 (MQ=255)
                                      ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAAt                                                                                                    <  2:816164/149‑1 (MQ=255)
                                           tGCTCATATGGCGCGTCGTTTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                                                                               <  1:613366/149‑1 (MQ=255)
                                           tGCACCGTTGTCGCTGCGTCTGTAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                                                                               <  1:88616/149‑1 (MQ=255)
                                                   tGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGt                                                                                       >  2:465344/1‑149 (MQ=255)
                                                   tGGCGCGGCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGCCATGTAAACCAGTATTCCCCGt                                                                                       <  2:782257/149‑1 (MQ=255)
                                                                gCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTACACCGACGTGACGGGCCGCATTGCACAGGCTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCCAAATTCCCCCACAGGAAATTgcac                                                                          >  2:650093/1‑146 (MQ=255)
                                                                 cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCTTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCa                                                                         <  1:112806/149‑1 (MQ=255)
                                                                 cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCGGCATTGCACAGACTCTGCTGAATCTGGACAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCCGTCa                                                                         >  1:449865/1‑149 (MQ=255)
                                                                 cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCa                                                                         <  1:618544/149‑1 (MQ=255)
                                                                   aGTGACTTCATATAAAGTGGGCAACCTGGCGTTCCTCGACGTAACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                                                                       <  2:842754/149‑1 (MQ=255)
                                                                     tCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACg                                                                                                              >  1:712085/1‑108 (MQ=255)
                                                                     tCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACg                                                                                                              <  2:712085/108‑1 (MQ=255)
                                                                        cTTCAGAGAAAGTGGGCAAGCTGGAGTGTCTCGACGTGACGGGCCGAATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACTGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGtg                                                                  <  2:376968/149‑2 (MQ=255)
                                                                         ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg                                                                 >  1:323924/1‑149 (MQ=255)
                                                                          tCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                <  2:660069/149‑1 (MQ=255)
                                                                             gagaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCAGCTGAATCAAGAAGGACACCCCCGCGCTATGACTCACCCGGACGGTATTCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg                                                             >  1:661956/1‑149 (MQ=255)
                                                                                        caatcgggCGTTCCTCGAAGGGACGGGCGGCATTGCACAGACTCTGCTGAATCTGGAATAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCCAAATTACCCGTCAGGAAATTGGTCAGTTTGTCAGTTGTTCTCGTGaaa                                                  <  2:782578/143‑1 (MQ=255)
                                                                                          tccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAAcc                                                <  1:366544/148‑1 (MQ=255)
                                                                                           ccTGGCGTTCTTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCg                                               <  1:797976/149‑1 (MQ=255)
                                                                                            cTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAACTTACCCGTCAGGAAATTGGTAAGATTGTCGGCTGTTCTCGTTAAACCga                                              >  2:366544/1‑148 (MQ=255)
                                                                                             tGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGACAAACAACCAGACGCTATGACTCACCCGGACGGTATTCAAATCAAAATTACCCGTTCGGCAATTGGTCAGATTTTCGGCTGTTCTCGTGAAAcactg                                             >  1:389953/1‑145 (MQ=255)
                                                                                              ggCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                            >  1:736569/1‑149 (MQ=255)
                                                                                                      tCGACGTGACGGGCCGCATTGCACAGACTATGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCAGTCAGGAAATTGGTAAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAtt                                    >  1:563290/1‑149 (MQ=255)
                                                                                                           gTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                               <  2:214972/149‑1 (MQ=255)
                                                                                                             gACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGa                             <  1:888082/149‑1 (MQ=255)
                                                                                                             gACGGGCCGCATTGCACAGACTCTGCTGAATCCGGAAATTCAACCGTACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGa                             <  1:698580/149‑1 (MQ=255)
                                                                                                                gggCCGCATTGCACAGACTCTGCTGAAGCTGTAAGAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAGTGTCGGCTGGTGTCGTGAAACCGTGGGACCCCTTCTGACGATGc                          >  2:7577/1‑149 (MQ=255)
                                                                                                                        ttGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGAt                  <  2:883713/149‑1 (MQ=255)
                                                                                                                          gCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa                <  2:180330/149‑1 (MQ=255)
                                                                                                                              aGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGCCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCACTCTAAAGATGCTGAAAGATCAGAAc            >  1:575276/1‑149 (MQ=255)
                                                                                                                                       tgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGACATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCCTTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc   >  1:197131/1‑149 (MQ=255)
                                                                                                                                        gAATCTTGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGACATTGTTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGCAGTTCAGAACCTGATCTCCg  >  2:234905/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                            
GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCG  >  NC_000913_3_bme_pgi/3486430‑3486714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A