Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F50 I0 R1
|
80 |
46.5 |
1794022 |
91.0% |
1632560 |
142.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,123,110 |
G→A |
100% |
P77L (CCA→CTA) |
ftsN ← |
cell division protein FtsN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,123,110 | 0 | G | A | 99.0%
| 83.4
/ ‑6.8
| 35 | P77L (CCA→CTA) | ftsN | cell division protein FtsN |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (16/17); minor base C (1/1); total (17/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGA > NC_000913/4122970‑4123241
|
gTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagt < 2:846330/143‑1 (MQ=255)
ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGGTCCGGCGTTGTCACTTCACCACCGGCTGAAGGTTCTGTGGGCGCGCGCGCTCCGGGCTGGCGACGTTCCAGCTCTTGAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg > 2:702986/1‑143 (MQ=255)
ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGCCTTTCCAGCTCTTTAATGTAGCGCCAGCGCTCCTCTGGTTTtagtg > 2:238262/1‑143 (MQ=255)
ttCAAGAAGCTGACGTTGCTCTGGTGTCAGTTGCTCCCGCGTTTTCACTTCCACACCGGCCGAAGGTTCTGTGGGCCCACGCACTCCCGGCTGGCCAGTTTTTATTCCTTTTATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 2:316377/143‑1 (MQ=255)
aagaagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTGCCAGGTCTTTAATGTAGCGCCCGCGTGCATCGGGTGTTAGGGGTa > 2:842083/1‑143 (MQ=255)
agCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTcc < 1:395167/143‑1 (MQ=255)
gCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCg > 1:524140/1‑143 (MQ=255)
gACGTTGTTCTGGTGACAGTTGCTCAGGCACTGGCACGCCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTCTCCAGCTCTTTAACGTCGCGCCAGCGTCCTTCTGGTTTCCGTGGTAGTCCGttt > 2:275551/1‑143 (MQ=255)
gttgttGTGGTGTCCGTTGCTCCGGGGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACACACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGGCCGTTTCCg < 2:857131/143‑1 (MQ=255)
tCTGGTGTCAGTTGCTCCGGCGTTTTCGCTGCGCCTGCGGCAGAGGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCAc < 2:331683/143‑1 (MQ=255)
ttGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCttt > 1:808965/1‑143 (MQ=255)
cTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCAGCTGGCGACTATCCAGCTCTTTAGTGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCGGGTCACTTACTTGCTTTGCa > 1:61051/1‑143 (MQ=255)
ccGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGc > 1:487688/1‑143 (MQ=255)
cGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCCTTCTGGCTTCGCACCGcct > 1:171279/1‑143 (MQ=255)
ttttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg < 1:458389/143‑1 (MQ=255)
ttcacttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTTCAGCGCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa > 1:697817/1‑143 (MQ=255)
cctcggccaccGGCAGAAGGGTCTGTGGGCGCACGCGCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTTTTCTGGTTTGAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGActc < 2:85296/137‑1 (MQ=255)
ttcacCACCGGCAGAAGGTTCTGTGGGCGGACGCACTCCCGACTGGGGACTTTCCAGCTCTTCAATGTAGCGCCAGCGTTCGTCTGGTTTTAGTGGTAGTCCGTTTCCGGTGACAATTCGGATTTGCAGAGTCTCGGATtctt > 2:218592/1‑143 (MQ=255)
accGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGCCCTCttctttct > 1:583900/1‑143 (MQ=255)
cGGCAGGAGGTTCGCTGGGCGCGCGCTCGCCCGGCTGGCGACTGTCCAGCGCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtg < 2:752572/143‑1 (MQ=255)
ctgtacgTTCTGTTGGCGCACGCACTGCCGGTTGTGGACTTTTCAGCTCTTTTTTGTGGCGCTAGCGTTCTTCTGTTTTTCGTCGTACTCCGTTTCCTGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGa < 2:125367/137‑1 (MQ=255)
cAGAAGGTGCTGTGGGCGCACGCACCCCCGGCTGGCGACTTTCCAGCTCTTTCATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGa < 1:238262/143‑1 (MQ=255)
gtgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGAGGTGGGCCGGTGGTACTGGTCGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTGCTTTCTTGTGCTGCGTAATGac > 1:847858/1‑142 (MQ=255)
gggggCGCACGGGCGCCCGGCTGGCGGCTTGCCGGCGCTTTGGTGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCGGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa < 2:266446/141‑1 (MQ=255)
ggCGCACGCGGTGCCGTCTGGCGACTTTCCGGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTa < 2:527358/143‑1 (MQ=255)
gcacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAg < 1:348259/143‑1 (MQ=255)
cgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCAGacc > 2:358761/1‑143 (MQ=255)
ggCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTTATGCGTAATGAAGTACAGACCACCGATaaa < 2:601632/143‑1 (MQ=255)
ggCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGGTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGCAGTACAGACCACCGGTAAAGGTc > 1:152059/1‑143 (MQ=255)
ttCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCAATAAAGGTCAaaaaaa > 1:521412/1‑143 (MQ=255)
ccAGCTCTTTAATGTAGCGCCAGCGTTCTTCAGGTATTAGCGGCACTCCCTTTCCGGTCACTTTCTGGCTTTGCAGCGTCGCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAGAGGGCACAAGAAcg > 1:896904/1‑143 (MQ=255)
aGCTCTTTAATGTCGCGCCAGCGCTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc < 1:388209/143‑1 (MQ=255)
tAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGGGTCTCGGACTCTTCTTTCTTGTGATGCGTGATGAAGTCGGGACCCCCGATAAAGGTCACCAGCAcggcgctagcc > 2:600828/1‑137 (MQ=255)
ggTAGCGCCTGCGTTCTTCTTGTTTTAGTGGTAGTGCGTTTCCGTTCACTTTCTGGCTTTGCAGGGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATa < 2:75788/142‑1 (MQ=255)
gTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAg < 1:759610/143‑1 (MQ=255)
gcgcCAGCGTTCTTCTGGTCTTAGTAGTAGTTCGATGCGGGTCACTTTCTGCCTTTGCAGCTTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGACACAAGAACGGCAGCAGCAATAGCGa > 2:492585/1‑143 (MQ=255)
gcgcCAGCGTTCTTCTGGGTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACGCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa < 1:304498/143‑1 (MQ=255)
tttctGGTTTGAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGAGAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGga < 2:164856/142‑1 (MQ=255)
cttgtGGTTTTAGTGGTAGTCTGTGTCCGGTCGCTTTCTGGTGTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGga < 2:362114/143‑1 (MQ=255)
|
GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGA > NC_000913/4122970‑4123241
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
