Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F91 I0 R1	49	33.8	1296440	89.8%	1164203	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,123,110	G→A	100%	P77L (CCA→CTA)	ftsN ←	cell division protein FtsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,110	0	G	A	100.0%	106.7 / ‑6.7	42	P77L (CCA→CTA)	ftsN	cell division protein FtsN
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (18/22); minor base C (1/0); total (19/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACC  >  NC_000913/4122971‑4123247

ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTGCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg                                                                                                                                        <  1:402940/143‑1 (MQ=255)

 tCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtgg                                                                                                                                       >  1:610493/1‑143 (MQ=255)

            aCGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTc                                                                                                                            <  1:40347/143‑1 (MQ=255)

            aCGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTc                                                                                                                            <  1:400233/143‑1 (MQ=255)

              gttgttTTGGTGTCAGGTGCTCCGGCGTTTTCACTTCCCCGCCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGGCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg                                                                                                                          <  2:99133/143‑1 (MQ=255)

              gttgttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCg                                                                                                                          <  1:577272/143‑1 (MQ=255)

                          tCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCAGTTTCTgg                                                                                                              >  1:305913/1‑143 (MQ=255)

                             gTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCTAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCtt                                                                                                           <  1:196028/143‑1 (MQ=255)

                              ttGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCttt                                                                                                          >  1:277160/1‑143 (MQ=255)

                               tGCTCCGGCGTTTTCGCTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTg                                                                                                         <  2:636924/143‑1 (MQ=255)

                                     ggCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTCCTTCTGGTTTTAGTGGTCGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGt                                                                                                   >  2:495734/1‑143 (MQ=255)

                                           ttcacttcgcCGCCGGCAGAAGGTTCTGAGGTCGCACGACTTAGCTGATGCCGACTTTCCAGCTCTTTAATGTAGCGCGAGCGTTCTGCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa                                                                                             <  2:556962/143‑1 (MQ=255)

                                               cttcacCGCCGGCAGAAGGTTCTGTGGGCGCACGCCCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCCTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGActct                                                                                         <  1:598249/143‑1 (MQ=255)

                                                   accaccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGTTCTGCAGCGTCTCCGACTcttctt                                                                                     >  1:265399/1‑143 (MQ=255)

                                                       ccGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTtctttctt                                                                                 <  1:446012/143‑1 (MQ=255)

                                                         ggctgcaGGTTCTGTGGGCGCCCGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCTCTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtgt                                                                               <  2:425634/137‑1 (MQ=255)

                                                              caGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGc                                                                          <  1:33533/142‑1 (MQ=255)

                                                                  ttCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTaa                                                                      >  1:518996/1‑143 (MQ=255)

                                                                      gtgGGCTCTCCCTCTCCGTGCTGGCGGCTTTCCAGATCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTACTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa                                                                  <  2:97038/143‑1 (MQ=255)

                                                                      gtcggCGCACGCACTCCCGGCTTGCGACTGTCCAGCTCTTTGGTGTTGCTACCTCCCCCTTCTGGTTTGAGACGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa                                                                  <  2:629156/140‑1 (MQ=255)

                                                                      gggggCGCATGCTCTCCCGGCTGGCGGCGTGCGAGCTCTTTACTGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTGTGGCTTTGCTGCGTCTCGGACTCTTCTTTCTGGTGATGCGTAATGaa                                                                  <  2:460580/141‑1 (MQ=255)

                                                                        gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGCTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTCGCTTTGCAGCGTCTCGGACTCTTCTCTCTTGTGATGCGTAATGAAGt                                                                >  2:467869/1‑143 (MQ=255)

                                                                         ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGGGATATGTTAATGTTATTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTa                                                               <  1:470846/143‑1 (MQ=255)

                                                                         ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTACTAAAGTa                                                               >  1:203426/1‑143 (MQ=255)

                                                                         ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAACGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCTTTCTGCGGTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTTATgtagta                                                               >  2:139312/1‑143 (MQ=255)

                                                                            gcacgcacTCCCGGGTGGCGACTTTGCAGCTCTTTAAGGGAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAg                                                            <  1:45775/143‑1 (MQ=255)

                                                                             cacgcacTCCCTGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTCTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGa                                                           <  2:321895/143‑1 (MQ=255)

                                                                              acgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCCGCGGTCTTCTGGTTTTCGTGTTAGTCCGTCTCCGTTCGCTTCCTGGCTTTGCAGCGTCTCGGACTCTCCTTTCCTGTGTTGCGCAATGAAGTAccgcc                                                          >  2:59008/1‑139 (MQ=255)

                                                                                 cccTCCCGGCTGGCGACTTTCCCGCTCTTTGATGGAGCGCCAGCGTTCTTCTGGTTTTACTGCTAGTCCGTGTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGCCTCTTCTTTCTTGGGATGCGTAATGAAGTACAGaccac                                                       <  2:524257/141‑1 (MQ=255)

                                                                                  acTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTATCGaccccc                                                      >  1:480469/1‑143 (MQ=255)

                                                                                         gtttgctaCTTTTCCGCTCTTTAATGTTGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAg                                               <  2:570005/136‑1 (MQ=255)

                                                                                          cTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGCCTCTTCCTTCTTGTGATGCGTAATGAAGTACAGACCACCGATACAgg                                              >  2:403579/1‑143 (MQ=255)

                                                                                                  ttaccAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGCTTTAGTGGTAGTGCGTTTCCGGTCACTTTCTGGCTTTGGAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGa                                      >  2:618912/4‑143 (MQ=255)

                                                                                                    tctaGCTTTTTAATGTAGTGCCAGCGTTCTTCTGGTTTTATTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTTGGACTCTTCTTTCTTGTGTTGCGTAATGATGTACAGACCACCGATAAAGGTCACAAGAAc                                    <  2:137314/140‑1 (MQ=255)

                                                                                                       aGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc                                 >  1:102283/1‑143 (MQ=255)

                                                                                                                  gTAGCGCCAGCGTTCTTCTGGACTTAGTGGTAGTCAGTTTCCGGTCACTTTCTGTCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTACTGAAGTACAGCCCACCGCTAAAGGCCACTGGACCGCCGgcggcacccg                      >  2:235153/1‑139 (MQ=255)

                                                                                                                   tAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGc                     >  1:405116/1‑143 (MQ=255)

                                                                                                                     gcgcGAGCGTTGTTCTGGTGTTAGTGGTAGTCCGTTTCCGGTCCGTTGCGGGCTTTGCAGCGTGTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa                   <  2:204990/143‑1 (MQ=255)

                                                                                                                     gcgcCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCCCCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa                   >  2:489092/1‑143 (MQ=255)

                                                                                                                        ccAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCTTTCTGGCTTTGCAGCGTCTCGGTCTCTTCCTTTTTGTGATGCGTGATGAAGTACAGACCACGGGTAAAGGTCACACGTGCGGGGGCAGCAATGGGGACCa                >  2:585536/1‑143 (MQ=255)

                                                                                                                            cGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGAc                  >  2:600843/1‑137 (MQ=255)

                                                                                                                            cGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGAc                  <  1:600843/137‑1 (MQ=255)

                                                                                                                              gtcttcaGGTCTTTGTGTTAGTCCGTTTCCTGTTCCTTTCTGGCGATTCAGCGTCTCGGACTCTTGGTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCgg          <  2:129456/142‑1 (MQ=255)

                                                                                                                                      gTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAGAGGTCACAGGAACGGCGGCAGCAATAGCGACCGTAGCGGGAGAAGcc  >  1:3845/1‑143 (MQ=255)

TTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACC  >  NC_000913/4122971‑4123247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A