Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F21 I1 R1	22	41.7	1602136	90.4%	1448330	141.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,110	G→A	P77L (CCA→CTA)	ftsN ←	cell division protein FtsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,110	0	G	A	95.0%	95.2 / NA	40	P77L (CCA→CTA)	ftsN	cell division protein FtsN
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/23); minor base C (2/0); total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCA  >  NC_000913/4122970‑4123250

gTTCAAGAAGCTCACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGACGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagt                                                                                                                                            <  1:570371/143‑1 (MQ=255)

  tCAAGGCGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtgg                                                                                                                                          <  2:799182/143‑1 (MQ=255)

    aagaagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTa                                                                                                                                        <  1:218582/143‑1 (MQ=255)

                ttgttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCCTGTAGCGCCCGCGTTCTTCTGGTTTTAGGGATAGTCCGTTGccag                                                                                                                            >  1:283035/1‑141 (MQ=255)

                      tGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTCTCCAGCTCTTTAATGTAGCGCCAGCCTTCTTCTGGTCTTCGTGGTAATCCATTTCCgctaacgt                                                                                                                      >  1:256058/1‑136 (MQ=255)

                        gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTc                                                                                                                    >  1:595298/1‑143 (MQ=255)

                           tCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTgg                                                                                                                 <  1:694870/143‑1 (MQ=255)

                             aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCATGTCGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCAGTCACTTTATTGCt                                                                                                               >  1:325018/1‑143 (MQ=255)

                              gTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGCCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTCGTCCGTTTCCGGTCACTTTCTGGCtt                                                                                                              >  1:272179/1‑143 (MQ=255)

                                 gCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGc                                                                                                           <  1:187911/143‑1 (MQ=255)

                                   tCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAg                                                                                                         >  1:655820/1‑143 (MQ=255)

                                    ccgccgCTTTCGCTTCACCTCCGGCAGAAGGTTCTTTGGGCTCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCATTTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTAACTGTGTGGCTTTGCAGc                                                                                                        <  2:59673/143‑1 (MQ=255)

                                     cGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCCGCGTGCTTCTGGTGTTCGTGGTGGTCGGTCTCCGGTCGCGTTCTGGCTTTgtcgcg                                                                                                       >  2:705110/1‑138 (MQ=255)

                                     cGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTACTGGTCGTCCGTTTCCGGTCACTTTCTGCCTTTGccccg                                                                                                       >  2:337136/1‑139 (MQ=255)

                                         gTTTTCACTTCACCACCGGCAGAAGGTGCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTGTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGtctc                                                                                                   <  1:337136/143‑1 (MQ=255)

                                         gTTTTCACTTCACCACCGGCAGAAGGCTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGtctc                                                                                                   <  1:465305/143‑1 (MQ=255)

                                          ttttCACTTCACCCCCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg                                                                                                  <  1:98540/143‑1 (MQ=255)

                                           tttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTcc                                                                                                                              <  2:291670/114‑1 (MQ=255)

                                           tttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTcc                                                                                                                              >  1:291670/1‑114 (MQ=255)

                                           tttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCgg                                                                                                 >  1:742/1‑143 (MQ=255)

                                                         cGGCAGAAGGTTCTGTGGGCGCTCGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtg                                                                                   <  1:309572/143‑1 (MQ=255)

                                                                    tCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAAt                                                                        <  1:21592/143‑1 (MQ=255)

                                                                     cTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTATGATGCGTAATg                                                                       >  1:748243/1‑143 (MQ=255)

                                                                          ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTa                                                                  <  1:604913/143‑1 (MQ=255)

                                                                          ggCGCACGCACTCCCGGCTGGCGACTTGCCAGGTCGTTAACGTAGCGCCAGCGTTCTTCTGGCCGTAGTGGTAGTCCGTTTCGCGTCACTTTTTGGCTTTGGAGCGGCTCGGCCTCTTCTCTCCTGTGATGCGTCATGACGtg                                                                  >  2:115513/1‑142 (MQ=255)

                                                                           gcgcACGCGCTCCCGGCTGGCGACTTGCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTAc                                                                 <  2:628556/143‑1 (MQ=255)

                                                                                     tCCCGGCTGGCGACTTTCGAGCCCTTTGATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGa                                                       <  2:237496/143‑1 (MQ=255)

                                                                                     tCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCGTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGa                                                       >  2:169966/1‑143 (MQ=255)

                                                                                        cGGCTGGCGATTTTCCAGCTCTTTAATGTAGCGCCATCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATaa                                                    <  2:645031/143‑1 (MQ=255)

                                                                                             ggCGCCCTTGCAGCTCGTTAATGTAGCGCCCGCGTTCTTCTGGTTTTAGTGGTACTCCGTTTCCTGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTTATGCTTAATGAAGTACAGACCACCGATAAAGGTc                                               <  1:455360/143‑1 (MQ=255)

                                                                                              gCGTCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGCTAGTCCGTGCCTGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTca                                              <  2:693482/143‑1 (MQ=255)

                                                                                                gACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATTCGCAACTAAACACAGACCACCGATAAAGGTcaca                                            <  2:655820/143‑1 (MQ=255)

                                                                                                   tttCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGTTGCGTGATGAAGTACAGACCACCGATCACGGGCACAAGa                                         >  2:53515/1‑143 (MQ=255)

                                                                                                         gCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATACAGGTCACAAGAAcggcg                                   >  1:86585/1‑143 (MQ=255)

                                                                                                          ctctTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGGGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggcgg                                  <  2:371178/143‑1 (MQ=255)

                                                                                                          ctctTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAGGTACAGCCCACCGATAAAGGTCACAAGAAcggcgg                                  >  1:62773/1‑143 (MQ=255)

                                                                                                             tttAATGTATCGCCAGCGTTCTTCTTGTTTTAGTGGTTGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGgcag                               <  1:123617/143‑1 (MQ=255)

                                                                                                                          cAGCGTTCTTCTGGTTTTAGTGGTAGTCCGGTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCAt                  <  2:595298/143‑1 (MQ=255)

                                                                                                                               ttcttcTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCgg             <  1:169966/143‑1 (MQ=255)

                                                                                                                                 cttccGGTTGTAGTGGTAGTCCGTGTCCGGGCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGga           <  1:311096/143‑1 (MQ=255)

                                                                                                                                    ctGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCAGACCACCGATAAAGGTCACACGAACGGCGGCAGCAATAGCGCCCATAGCTGgagca        >  2:557950/1‑141 (MQ=255)

                                                                                                                                     tgtttttAGTGGTAGTCCGTTTCGCGTCACTTTCTGGCTTTGCAGCGTCTCTGACTCTTGTTTCTTGTGCTTCTTAATGAACTACAGACCACCGCTAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGaaa       <  2:283035/140‑1 (MQ=255)

                                                                                                                                          tgggtggtAGTGCGTTGGCGGTGCTTTTGTGGCTTTGCAGCGTCTGGGACTCTGCCTGCTTGTGATGCGTAAGGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCgca  <  2:383959/141‑1 (MQ=255)

GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCA  >  NC_000913/4122970‑4123250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A