breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 125,387 C→T 100% Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 475,971 C→G 14.4% intergenic (+20/+11) ybaY → / ← atl PF09619 family lipoprotein YbaY/DNA base‑flipping protein
RA 475,972 C→G 14.4% intergenic (+21/+10) ybaY → / ← atl PF09619 family lipoprotein YbaY/DNA base‑flipping protein
RA 569,160 G→T 5.2% D87Y (GAC→TAC) ‡ ybcK → DLP12 prophage; putative recombinase
RA 569,161 A→C 5.0% D87A (GAC→GCC) ‡ ybcK → DLP12 prophage; putative recombinase
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 709,461 C→T 9.2% G376G (GGC→GGT chiP → chitobiose outer membrane channel
RA 710,595 G→T 100% P18T (CCT→ACT)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 759,250 G→T 100% R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 800,807 G→T 100% A17S (GCG→TCG)  ybhH → putative isomerase YbhH
RA 1,166,293 C→T 100% A70V (GCC→GTC)  ndh → NADH:quinone oxidoreductase II
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,388,283 C→T 14.8% intergenic (+22/+22) tyrR → / ← tpx DNA‑binding transcriptional dual regulator TyrR/lipid hydroperoxide peroxidase
RA 1,388,284 A→T 14.7% intergenic (+23/+21) tyrR → / ← tpx DNA‑binding transcriptional dual regulator TyrR/lipid hydroperoxide peroxidase
RA 1,388,285 A→G 17.3% intergenic (+24/+20) tyrR → / ← tpx DNA‑binding transcriptional dual regulator TyrR/lipid hydroperoxide peroxidase
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC 2,392,615 Δ3 bp 100% coding (425‑427/1530 nt) nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,484,369 A→G 10.2% *205* (TAA→TAG evgA → DNA‑binding transcriptional activator EvgA
RA 2,568,146 G→A 11.7% intergenic (‑39/+178) eutH ← / ← eutG putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization
RA 2,568,147 C→A 13.3% intergenic (‑40/+177) eutH ← / ← eutG putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization
RA 2,568,153 A→T 11.4% intergenic (‑46/+171) eutH ← / ← eutG putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization
RA 2,568,154 A→T 13.7% intergenic (‑47/+170) eutH ← / ← eutG putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization
RA 2,568,160 T→G 11.2% intergenic (‑53/+164) eutH ← / ← eutG putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization
RA 2,568,161 T→C 13.0% intergenic (‑54/+163) eutH ← / ← eutG putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization
RA 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,761,623 C→A 9.4% V640L (GTA→TTA)  abpB ← CP4‑57 prophage; putative helicase YfjK
RA 2,778,123 G→T 13.0% intergenic (+341/+23) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 2,778,124 A→C 12.5% intergenic (+342/+22) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,380,208 G→A 13.3% A6A (GCG→GCA yhcB → conserved protein YhcB
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,602,701 T→C 27.5% Q16R (CAG→CGG)  ftsE ← cell division protein FtsE
RA 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA
RA 3,719,876 C→A 81.9% intergenic (+108/‑173) yiaG → / → cspA putative DNA‑binding transcriptional regulator YiaG/cold shock protein CspA
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,886,756 G→A 8.1% intergenic (+34/‑72) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,886,757 T→C 9.0% intergenic (+35/‑71) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,900,410 A→T 9.6% P56P (CCT→CCA yieL ← putative hydrolase YieL
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,114,431 G→A 100% N14N (AAC→AAT fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,123,110 G→A 100% P77L (CCA→CTA)  ftsN ← cell division protein FtsN
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 19.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423753–3424530 3424530 1–778 11 [10] [10] 11 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 8607629 (0.890)3 (0.100) 3/256 NT 9.7% coding (447/1725 nt) ilvI acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
?NC_000913 = 86085 28 (0.890)coding (456/1725 nt) ilvI acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
* ? NC_000913 167280 =NA (NA)3 (0.100) 3/242 NT NA intergenic (+16/‑204) mrcB/fhuA peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB/ferrichrome outer membrane transporter/phage receptor
?NC_000913 167307 = NA (NA)intergenic (+43/‑177) mrcB/fhuA peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB/ferrichrome outer membrane transporter/phage receptor
* ? NC_000913 = 315572NA (NA)4 (0.130) 4/244 NT NA noncoding (344/1255 nt) IS3 repeat region
?NC_000913 = 315588 NA (NA)noncoding (360/1255 nt) IS3 repeat region
* ? NC_000913 = 316093NA (NA)3 (0.090) 3/258 NT NA noncoding (865/1255 nt) IS3 repeat region
?NC_000913 = 316135 NA (NA)noncoding (907/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1300 (40.230) 242/262 NT 98.1% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 26 (0.800)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 745824 =27 (0.830)3 (0.100) 3/254 NT 10.5% coding (660/735 nt) pxpA 5‑oxoprolinase component A
?NC_000913 745854 = 25 (0.800)coding (690/735 nt) pxpA 5‑oxoprolinase component A
* ? NC_000913 1207790 =20 (0.610)12 (0.420) 12/232 NT 39.6% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 19 (0.660)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780518 (0.550)12 (0.420) 11/232 NT 40.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 19 (0.660)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1417458 =25 (0.770)3 (0.100) 3/244 NT 12.2% intergenic (‑72/+30) recE/racC Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific/Rac prophage; protein RacC
?NC_000913 1417483 = 20 (0.660)intergenic (‑97/+5) recE/racC Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific/Rac prophage; protein RacC
* ? NC_000913 4189862 =30 (0.920)3 (0.100) 3/254 NT 9.5% coding (77/540 nt) yjaZ conserved protein YjaZ
?NC_000913 4189894 = 28 (0.890)coding (109/540 nt) yjaZ conserved protein YjaZ
* ? NC_000913 = 4470450NA (NA)4 (0.130) 3/250 NT NA noncoding (20/29 nt) other REPv339b
?NC_000913 = 4470461 NA (NA)noncoding (9/29 nt) other REPv339b