Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F50 I1 R1 21 62.9 2410612 90.0% 2169550 142.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,114,431 G→A N14N (AAC→AAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,4310GA97.9% 135.7 / NA 48N14N (AAC→AAT) fprflavodoxin/ferredoxin‑NADP(+) reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/19);  minor base C (1/0);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAAT  >  NC_000913/4114291‑4114569
                                                                                                                                            |                                                                                                                                          
ggATTATCGGGCGAGTTAACATACGAGTAGGCGCGCTGGACGCGTTCGCCGTCCATTAAAAGCCCAAGCTCGGGAGATTGCGCGGCGGTACACGGAAGCACGGGGGCGGGAACGGGAGGACGACACAGCGCGACGGCCCAggc                                                                                                                                          >  2:515986/1‑141 (MQ=255)
 gATTGTCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGGCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTc                                                                                                                                         <  1:770747/143‑1 (MQ=255)
 gATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTc                                                                                                                                         >  1:779015/1‑143 (MQ=255)
      tCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGGAc                                                                                                                                    >  1:1039497/1‑143 (MQ=255)
        gggCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACtt                                                                                                                                  >  2:1032496/1‑143 (MQ=255)
         ggCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCACTCCTGCACttt                                                                                                                                 >  2:999549/1‑143 (MQ=255)
          gCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCAGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGAATAAACAGGGCGTCGGTCCAATTCTGCACTTTa                                                                                                                                >  1:1086439/1‑143 (MQ=255)
               cgtgcATGGGAGGAGGCGCGCTTGACGCGTTCGCCGTCGATTTCGAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACGAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGAc                                                                                                                           <  2:1187558/139‑1 (MQ=255)
                  aCATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACttt                                                                                                                        <  1:464197/143‑1 (MQ=255)
                     tagccgTACGCGCGCTGGACGCCTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGcc                                                                                                                     <  1:461604/138‑1 (MQ=255)
                       ggAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTg                                                                                                                   <  2:10690/143‑1 (MQ=255)
                               cgcgcTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGCCTTTGCCTGTTACCCat                                                                                                           >  1:234158/1‑142 (MQ=255)
                                         gcgTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCGATTCTGCACTTTAGTGACTGTGCCAGCTACGAAATCAGCCGTgg                                                                                                 >  1:383515/1‑142 (MQ=255)
                                             gcGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGttttt                                                                                             <  1:358311/142‑1 (MQ=255)
                                                 cGTCGATTTCAAGGCCAAGCTTGGTAAATTGCTCGGCGGTAAACGGAAGCACGGGGGCGTAACCGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTCTACCATCCGCCATGTCTTTCTcc                                                                                         >  2:368269/1‑143 (MQ=255)
                                                    cGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGTGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGt                                                                                      >  2:897500/1‑143 (MQ=255)
                                                     gATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGtt                                                                                     <  1:90894/143‑1 (MQ=255)
                                                      ttttgcaGGCCAAGCTTGGTAACTTGCCCGGCGGTAAGCGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCTCTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGttt                                                                                    <  2:953914/137‑1 (MQ=255)
                                                        ttCATGGCCAAGCTTGGTAAATCGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttg                                                                                  <  1:1074980/143‑1 (MQ=255)
                                                        ttCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttg                                                                                  <  1:968222/143‑1 (MQ=255)
                                                            aGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttgattg                                                                              >  2:1106953/1‑143 (MQ=255)
                                                              gCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGGCTGTTACGCAATCAGCCATGTTTTTCTCCTGGTTTGAGTGAc                                                                            >  2:184704/1‑143 (MQ=255)
                                                                cAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCCGTTTTGATTGACtt                                                                          >  2:674625/1‑143 (MQ=255)
                                                                  aGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAAGTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGc                                                                        <  1:327730/143‑1 (MQ=255)
                                                                   gCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGcc                                                                       <  1:184704/143‑1 (MQ=255)
                                                                    cTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGttt                                                                                               <  2:286378/118‑1 (MQ=255)
                                                                    cTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGttt                                                                                               >  1:286378/1‑118 (MQ=255)
                                                                       ggTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCCATCAGCCATGTTTTTCTCCTGCTTTGCTTGACTTGCCTTAt                                                                   >  2:897008/1‑143 (MQ=255)
                                                                        gtaacgtGCCCGGCGGTAAGCGGAACCACGGGGGCGTGAACGGCGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATc                                                                  <  2:1060107/137‑1 (MQ=255)
                                                                          aaaTTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCtt                                                                >  1:586052/1‑143 (MQ=255)
                                                                           aaTTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTc                                                               >  1:245802/1‑143 (MQ=255)
                                                                                 ccGGCGGTAAACGGAAGCACGGGGGCGTGAACTGTGCGACTAAACCGGGCGGCCGGCAAATTGGGCCCTTAGGAGACTTTGCCTGTTACCCAAGCAGACATTTTTTTTGTCTGTTTTGATGCACTCGCTTTATCTTCGtttcc                                                         >  2:872684/1‑140 (MQ=255)
                                                                                 ccGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGCCGGTCAAATTCTGCACATTAGTGACTTTGGCTGTTACCCAATCAGCCATGTTTTTCTTCTGTTTTTATTGACTTGCCTTATCTTCGTtctg                                                         >  2:179922/1‑142 (MQ=255)
                                                                                 ccGGCCGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCCCTTTAGTGACTTTGCCTGTTACCCAATCAGCCAGGTTTTTCTCCTGTTTTGATTGACTTGTCTTATCTTCGTtctc                                                         >  2:996787/1‑143 (MQ=255)
                                                                                          aaCGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACaaaa                                                <  1:1032496/143‑1 (MQ=255)
                                                                                               aaGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACGTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAGAtt                                           >  1:451040/1‑143 (MQ=255)
                                                                                                 gCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTGTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTcc                                         <  2:1140221/143‑1 (MQ=255)
                                                                                                           aacaagggTGAGACTAAACTGGTCGTCGGTCCAATTCTGCACTGTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCATGTGTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAg                               <  2:121441/137‑1 (MQ=255)
                                                                                                              aaCGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGAc                            <  1:447953/143‑1 (MQ=255)
                                                                                                                 ggTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTATCCGAACAAAAAAATTCCAGTCCCGAAGGACggg                         >  1:423987/1‑143 (MQ=255)
                                                                                                                       aCTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCGGAAGGACTGGAAgacc                   >  1:669996/1‑140 (MQ=255)
                                                                                                                         tAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCa                 >  1:956744/1‑143 (MQ=255)
                                                                                                                         tAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAAC‑AAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCaa                >  2:435816/1‑143 (MQ=255)
                                                                                                                         tAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTGGTTTCCGGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCa                 >  1:757125/1‑143 (MQ=255)
                                                                                                                          aaaCAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTAGATGGCTCaa                >  1:581905/1‑143 (MQ=255)
                                                                                                                           aaCAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGTACAGAAAAATTCCAGTCCCGATGGCCTGGAAGGcaccat               >  2:464256/1‑138 (MQ=255)
                                                                                                                                      ggTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatcaaatca    >  1:1018034/1‑143 (MQ=255)
                                                                                                                                      ggTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTAGCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatcaaatca    <  1:821531/143‑1 (MQ=255)
                                                                                                                                        tCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAaatcaat  >  1:911185/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAAT  >  NC_000913/4114291‑4114569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A