Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F21 I1 R1 22 41.7 1602136 90.4% 1448330 141.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,114,431 G→A N14N (AAC→AAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,4310GA98.2% 148.5 / NA 55N14N (AAC→AAT) fprflavodoxin/ferredoxin‑NADP(+) reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/29);  minor base C (0/1);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAA  >  NC_000913/4114291‑4114564
                                                                                                                                            |                                                                                                                                      
ggATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAGACGGAAGCACGGAGGCGTGAACGGTGAGACTCAACAGGGCGTCGGTCCAAtt                                                                                                                                      >  1:487046/1‑143 (MQ=255)
     aTCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCa                                                                                                                                 >  2:333827/1‑143 (MQ=255)
     aTCGGGCGAGTTTACATAGGAGTAGGCGCGCGGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCa                                                                                                                                 <  1:138323/143‑1 (MQ=255)
            gAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCAAGGGGGCGGGAACGGTGAGACTAAACAGGGCGTCGGTCCATGTCGGCACTTTAGt                                                                                                                          >  2:648319/1‑143 (MQ=255)
              ggttACAGTGGAGTAGGCGCGGTGGACGCGTTGGCCGGCGACTTCAAGGGCAAGCTGGGTAAAGTGCCCGGGGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGGCTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGa                                                                                                                        <  2:709040/141‑1 (MQ=255)
                    aTAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGc                                                                                                                  <  1:788028/143‑1 (MQ=255)
                    aTAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATATCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGc                                                                                                                  >  1:516277/1‑143 (MQ=255)
                    aTAGGAGGAGGCGCCCTGGACGCGTTCGCCGTCGCTTTCAAGGCTAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGc                                                                                                                  <  2:94853/143‑1 (MQ=255)
                      aGGAGTAGGCGCGCGGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCCATTCTGCACTTTAGTGACTTTGCCt                                                                                                                <  2:192180/143‑1 (MQ=255)
                        gAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGGACTTTGGTGACTTTGCCTGt                                                                                                              >  1:696162/1‑143 (MQ=255)
                           tAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACCGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTAc                                                                                                           >  1:204111/1‑143 (MQ=255)
                              gcgcgcTGGACGCGTTTGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCa                                                                                                        >  2:798086/1‑143 (MQ=255)
                                 cgcTGGACGCGTGCGCCGTCGATTTCAAGGCCAGGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATc                                                                                                     <  2:206137/143‑1 (MQ=255)
                                   cTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACTCAATAAg                                                                                                   >  1:491460/1‑143 (MQ=255)
                                       atgcgTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATGCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCAt                                                                                               <  2:21166/141‑1 (MQ=255)
                                       aCGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCGAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCAt                                                                                               >  2:554766/1‑143 (MQ=255)
                                            ttCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATGAGCAAtctagt                                                                                          >  2:40900/1‑138 (MQ=255)
                                             ccGCCGTCGATTGCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTAGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGttttt                                                                                         <  1:11957/142‑1 (MQ=255)
                                              cGCCGTCGACTTCAAGGCCCAGCCTGGTAAATTGCCCGGCGGTAAGCGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGCGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTtc                                                                                        <  1:554339/143‑1 (MQ=255)
                                                 cGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTcc                                                                                     <  1:57518/143‑1 (MQ=255)
                                                  ggcGATTTCACGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCt                                                                                    <  1:235759/141‑1 (MQ=255)
                                                      aTTTCAAGGACAAGCTTGGTAAATTGCCAGGCGGTAAACGGAAGCACGGGAGCGTGAACGGTGAGACTAAACAGAGCGTCGGTACAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAAACACCCATGTTTCTCTCCttttg                                                                                >  2:726328/1‑142 (MQ=255)
                                                        ttCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttg                                                                              >  1:736000/1‑143 (MQ=255)
                                                           aaGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttgat‑t                                                                          >  1:427206/1‑143 (MQ=255)
                                                           aaGGCCAAGCTTAGTAAGTTGCTCGGCGGTAAACGGAAGCACGGTGGCGTGAACTGTGAGACTTAACAGGTCGTCGGTCCAATTCTGCACTTTAGTAACTATACCTGTTACCCAATCAGCCATGTTTTTCTCCTTTTttgat‑t                                                                          <  2:96878/143‑1 (MQ=255)
                                                             ggCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGGACGGTGAGACTAAACAGGGCGTCGGTCCCATTCTGCGCTGTAGTGACTTTGCCTGTTGCCCAATCAGCCATGTGTTTCTCCTGTTttgacgga                                                                         >  1:70918/1‑139 (MQ=255)
                                                                cAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCCCGTTAGTGCCTTTGCCTGTTACCCACTCAGCCATGTTTTTCTCCTGTTTTGCT‑TGACtt                                                                     >  1:725119/1‑143 (MQ=255)
                                                                 aaGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGGT‑TGACTTg                                                                    >  2:145243/1‑143 (MQ=255)
                                                                    cgtGGTAAACTGCCCCGCGGTAAACTGCAGCACGGGGGCGTGAACGGTGATACTAAACCGGCCGTCCGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGTCTTGCCt                                                                 <  2:612994/141‑1 (MQ=255)
                                                                        gTAGATCGCGCGGCGGTAAACGGAAGGACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAAGTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGACATGTTTTTTGCCTGTTTTGAT‑TGACTTGCCTTATc                                                             <  2:487046/143‑1 (MQ=255)
                                                                        gTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGCT‑TGCCTTGCCTTATc                                                             >  1:48441/1‑143 (MQ=255)
                                                                         tAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTCGGGACTTTGCCTGTTACCCAGTCAGCCACGTTTTTCTCCTGTTTTGAT‑TGGCTTGCCTTATCt                                                            >  1:598813/1‑143 (MQ=255)
                                                                           tactGCCCGGCGGTCACCGCAAGCACTGGGGCGTGAACGGTGAGACTCAACAGGGCGTCGGTCCAATTCTGCACCCTAGTGACTTTGCCCGTTACCCAATCAGCCATGTCTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTc                                                          <  1:115213/140‑1 (MQ=255)
                                                                                 ccGGCGGTAACCGGAAGCACGGGGGCGTGAACGGTGAGTCTAAACTGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTtctc                                                    <  2:305165/143‑1 (MQ=255)
                                                                                 ccGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttg                                                                              <  2:751774/118‑1 (MQ=255)
                                                                                 ccGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTttg                                                                              >  1:751774/1‑118 (MQ=255)
                                                                                  tggcggTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTcc                                                   <  1:381950/142‑1 (MQ=255)
                                                                                  cggcggTACACGGAGGCACGGGGGCGTGAACGGTGGGACTGAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTcc                                                   <  2:584559/143‑1 (MQ=255)
                                                                                  cggcggTAAACTGAAGCACGGGCGCGTGAACGGGGAGACTAGACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTCACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTcc                                                   <  1:74251/143‑1 (MQ=255)
                                                                                     cggTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGaa                                                <  1:432373/143‑1 (MQ=255)
                                                                                          aaCGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAATACTGCACTTTAGTGACTTTGCCTGTTACCAAATCAGCCATGTTTTTCTCGTGTTTTGAT‑TGACTTGCATTATCTTCGTTCTCCGAACaaaa                                           >  1:262525/1‑143 (MQ=255)
                                                                                           aCGGAAGCACTGGTGCGTGAACGGTGAGTCTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTATCCGAACaaaaa                                          <  2:248744/143‑1 (MQ=255)
                                                                                                  cACGTGGGCGTGAACGGTGAGACTAATCAGGTCGTCTGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCa                                   <  1:48307/143‑1 (MQ=255)
                                                                                                  cACGGGGGCGTGCACGGTGAGACTAAACAGGGCTTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCa                                   <  1:147383/143‑1 (MQ=255)
                                                                                                          cgttaccGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGTCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGaa                           <  1:28189/137‑1 (MQ=255)
                                                                                                             gcaCGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGa                        <  1:720936/141‑1 (MQ=255)
                                                                                                             gAACGGTGGGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGa                        >  1:350951/1‑143 (MQ=255)
                                                                                                             gAACGGTGAGACTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCCACT‑ATCAGATACTGCCTGCCTTATCTTCGTTCTCCGCACAAAAAAATTCCAGTCCCGAAGGa                        >  1:182091/1‑143 (MQ=255)
                                                                                                              aaCGGTGAGACTACACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTGCCCACTAAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATGTTCGTTCTCCGAACAACAAACTTCCAGTCCCGAAGGAc                       >  1:438362/1‑143 (MQ=255)
                                                                                                                     agaCTAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTGATCTTCGTTCTCCGAAAAAAAAAATTCCAGTCCCGAAGGACTGGAAgg                <  1:45504/143‑1 (MQ=255)
                                                                                                                         tAAACAGGGCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCa            >  1:465097/1‑143 (MQ=255)
                                                                                                                               gggCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTc                                     >  2:645298/1‑112 (MQ=255)
                                                                                                                               gggCGTCGGTCCAATTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTc                                     <  1:645298/112‑1 (MQ=255)
                                                                                                                               gggCGTCGCTCCAACTCTGCACTTTAGTTACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTGGAT‑TGACTTGCCTTATCTTCGTTCTACGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGat      <  2:70918/143‑1 (MQ=255)
                                                                                                                                   gTCGGTCCACTTCGGCACTTTAGTGACTGTGCCTGTTACCCGATGTGCCCTTTGTATCTCCTGTTTTTTT‑GGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatcaaa  <  2:777438/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
GGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGAT‑TGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAA  >  NC_000913/4114291‑4114564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A