Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F2 I0 R1 166 6951.0 8192394 98.4% 8061315 136.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 20.0% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC20.0% 157.2 / 6.4 159Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (42/60);  new base C (11/14);  total (77/82)
Fisher's exact test for biased strand distribution p-value = 8.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440197‑4440473
                                                                                                                                         |                                                                                                                                           
gaaCAGCATCCGCATCAACCGGTAGCACCGCAGCATCAATATCATCAGACGCACCATCCTGTAGCCCCTGATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACAGCAGGCGCATTATCAGCAGCCGCAATATCAACa                                                                                                                                           <  7:953816/138‑1 (MQ=21)
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTGGAACCGCAGCCGCAGTAACAGCAGCCGCAATATCACCa                                                                                                                                           >  5:147586/1‑140 (MQ=21)
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAGCa                                                                                                                                           >  1:587603/1‑140 (MQ=255)
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATAAGCAGCCGCAACATCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAGCa                                                                                                                                           >  7:899006/1‑140 (MQ=21)
  aCAGCAGCCGCAACAACCGGTAGCACCGCATCAGCAATATCAGCAGCCGCAACAACAGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                                         <  1:1044262/140‑1 (MQ=255)
  aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGACGCAACTACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                                         <  3:128791/140‑1 (MQ=255)
      ccgcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCCCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCTCAGCCGCAGTATCCGCAGCAGCATAATCAGCAGACGca                                                                                                                                     >  1:802332/3‑140 (MQ=18)
      cagcCGCACCCCCCCGAAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGAAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCGCAATATCATCAGCCGca                                                                                                                                     <  8:492683/140‑1 (MQ=18)
        gctgCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCATCCGCAGTAACAGCAGCCGAAAAATCAGCAGCCGcaac                                                                                                                                   >  3:485273/4‑140 (MQ=21)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCACCCGCAACAACCGGTAGCCCCGCAGCCGCAGTATCAGCCGCCGCAACAACCGGTTCCACCGCAGCCGCAGTATCAGCAGCAGCAAAATAATCAGCCGcaac                                                                                                                                   >  3:200451/1‑140 (MQ=21)
         ccGCAACAACCGGTAGCACCGCAGCCGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCAGCGGCATAATCCGCAGCCGcaaca                                                                                                                                  >  8:577750/1‑140 (MQ=18)
         ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                  <  6:309705/140‑1 (MQ=255)
         ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTCCACCTCAGCCGCAGTATCAGAAGCCGCAAAATCCTCAGCCGcacaa                                                                                                                                  >  8:720535/1‑137 (MQ=18)
         ccGCAACAACCGGACGCACCGCAGCAGCTATATCACCAGCCGCAACAACCGGTAGAACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCACTAACAGCAGCCGCATTATCAACAGCCGcaaca                                                                                                                                  >  7:848483/1‑140 (MQ=21)
           gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACAGCAGCCGCAGTATCAGCAGCAGCAACATCCGGTGGCACCGCAGCCGCAGTATCATCCGCCGCAAAATCAGCAGCCGCcacaac                                                                                                                                >  2:613323/1‑140 (MQ=18)
                 acCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTg                                                                                                                          <  3:27227/140‑1 (MQ=255)
                   ctgTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCa                                                                                                                        <  2:571592/138‑1 (MQ=255)
                           ccGCAGCAGCAACATCCCCAGCAGCAACAACCTGTAGCACCGCCGCCGCATTATCAGCAGCCGCAACCACCGGATGCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCgcagca                                                                                                                <  4:446546/140‑1 (MQ=21)
                            cGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCATTAGCACCAGCCGCCTCAACCCGTTGCACCgcagctg                                                                                                               >  3:739914/1‑138 (MQ=18)
                            cGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATTATCAGCAGCCGCAACAACCGGTTGCACCgcagcag                                                                                                               >  1:222900/1‑140 (MQ=255)
                            cGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCACCAGCCGCAACAACCGGTTGCACCgcagcag                                                                                                               >  1:229653/1‑140 (MQ=255)
                             gccgcagcaATAGCAGCAGCCGCAACAACCGGAAGCACCGCAGCCGAAGTATCAGCAGCCGCAACAACTGGATGCACCGCAGCAGCAGTATCAGCAGCCGCAATCTCACCAGCCGCAACAACCGGTTGCACCgcagcagc                                                                                                              <  4:798378/137‑1 (MQ=21)
                              cagcagcaATATCCGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAAGATCAACCGCCGCCACAACCGGATGCACCgcagcagca                                                                                                             >  1:65771/1‑140 (MQ=21)
                              cagcagcaATATCAGCATCCCCACCACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCACCAACCGGTTGCACCGCAGCCGCAGTCTCAGCAGCCGCCATATCACCCGCCGCAACAACCGGTTGCAGCgccgcagcg                                                                                                             >  7:553936/1‑139 (MQ=21)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagca                                                                                                             >  6:451402/1‑140 (MQ=255)
                                    caATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTCTCCGCAGCCGCAAACTCAGCCGCCGCCACCACCGGATGCCCCGCAGCAGCAAtctcc                                                                                                       >  2:829475/1‑139 (MQ=17)
                                    caATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGACCCGCAGCCGCAGTATCAGCTGCCGCAATATCAGAAGCAGCCTCATCCGGTTCCACCgcagcagcataatcc                                                                                                       >  7:806297/1‑134 (MQ=18)
                                       tatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                                    <  3:299907/140‑1 (MQ=255)
                                              agcCGCAACCTGCGGGAGGACCGCAGCCGCAGTAGCAGCAGCCGGAAGAACCGCAGGCACCGCTGCCGCAGTATCAGCAGCCGCAATTTCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  3:113989/140‑1 (MQ=25)
                                              agcCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACAGGTTGCCCCGCAGCCCCAGGATCAGCAGCCGCAAACTCAACCGCCGCAACAGCCGGGTGCGCCGCGGCTGCAAGATCAGCCGCcagga                                                                                             >  8:78799/1‑137 (MQ=17)
                                              agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCACCACCCGGTGGCACCGCCGCCGCAGTATCCGCAGCCGCAATATCAGCAGCCGCAACATCCGGATGCCCCGCAGCAGCAATATCCgccgccgcca                                                                                             >  8:223761/1‑136 (MQ=18)
                                                ccGCATCATCCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCTCAGTATAAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  4:886095/140‑1 (MQ=25)
                                                ccGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  5:903918/140‑1 (MQ=255)
                                                ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCACAGCAGATGCAATATCAGCAGCCacaaca                                                                                           >  5:431691/1‑140 (MQ=255)
                                                ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  5:784283/140‑1 (MQ=255)
                                                ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAAAAGACTCAACAGCCGGATGCCCAGCAGCAGCAAAATCAGACGACacaaca                                                                                           >  2:46680/1‑140 (MQ=18)
                                                ccGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  3:405704/140‑1 (MQ=255)
                                                ccGCAACAAACGGTATCACCGCAGACGCAGTATCAGCAGGCGCAACAACCGGTTGAACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  1:994156/140‑1 (MQ=37)
                                                cagCAACAACCGGTAGATCCGCAGCAGCAGGCTGAGCAGCAGCAAGATCCGGTTGCACCGCTGCCGCAGTATCATCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  1:112193/138‑1 (MQ=25)
                                                 cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCAGCCACAACCGGTTGACCCGCAGCAGCATTATCAGCCGACGCAACAg                                                                                          >  8:161617/1‑140 (MQ=18)
                                                 cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagcag                                                                                                   <  7:508699/131‑1 (MQ=255)
                                                  gCAACATACGGTCTCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCTGTTGCACCGCAGCCGAAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                         <  3:551778/140‑1 (MQ=37)
                                                  gCAACAACGGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                         <  5:522769/140‑1 (MQ=255)
                                                  gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                         <  1:731558/140‑1 (MQ=255)
                                                      caacCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGt                                                                                     <  8:542090/140‑1 (MQ=255)
                                                        ccctgTACCACCGCATCCTCAGGATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTa                                                                                   <  1:105968/136‑1 (MQ=37)
                                                        acctgaaGCACCGCAGCCGCAGTATAAGCAGCAGCAAGATCCGGATGCACCGCAGTCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTa                                                                                   <  4:17667/134‑1 (MQ=37)
                                                           gctAGCACCGCAGCCGCAGTCTCAGCTGCAGCAACAACCGGTTGCACCGCAGCCGCAGTCTCAGCAGCAGCATTATCAACAGCCGCAACCACCGGTTGCACCGCAGCAGCAATTTCAGCAGCCACAACAGCCGGTTacac                                                                                <  7:632210/138‑1 (MQ=37)
                                                              aGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCATTATCCCCAGCCGCAACAACCGGTTGACCCGCAGCAGCAACATCAGCAGCCGCAACAGCCGGGTACAcagc                                                                             >  3:188346/1‑140 (MQ=25)
                                                                cACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACTTCCGGATACCcagcag                                                                           >  7:232542/1‑140 (MQ=37)
                                                                cACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACCcagcag                                                                           >  1:412664/1‑140 (MQ=255)
                                                                 ttcgcatcctcagTATCAGCAGCCGCAACTACCTGATGCACCTCAGCCGCAGTATCAGGAGGAGCATTATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                          <  2:479809/138‑1 (MQ=25)
                                                                 tccgcagcagcatTATCAGCAGCAGCAACATCCGGTCGCAGCGCAGCCGCAGTATCAGCAGCCGCAAGATCAGCAGCCGCCACACCCGGTTTCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                          <  7:482075/139‑1 (MQ=21)
                                                                  ccgcagccgctgTATCAGCAGCCGCAACTACCGGAAGCACCGCATCCGCAGGATCTGCAGCCTCAATATCAACAGCCGCAACTCCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  5:411180/140‑1 (MQ=37)
                                                                  ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  4:349319/140‑1 (MQ=255)
                                                                  ccgcagccgcagGATCAGCAGCCGCAACCTCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  2:961183/140‑1 (MQ=38)
                                                                   cgcagccgcagTCTCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  2:578696/140‑1 (MQ=255)
                                                                   cgcagccgcagTATCAGCTGCCGGAACACCCGGTTGCACCGCAGCCTCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  1:454869/140‑1 (MQ=255)
                                                                   cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  2:714783/140‑1 (MQ=255)
                                                                   cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCCCAGCCGCAGTATCAGCAGCCGCAATATCCCCAGCCGCAACAACCGGTTCCACAGCAGCAGCAAAAACAGCAGACACCACAGCCGGTTACAcagcctcag                                                                        >  7:647934/1‑135 (MQ=25)
                                                                     cagccgcatTAGCTGCAGCATCATCAACCTGATGCACCTCATCTGCATCATCAGCAGCCGCAATATCAACAGCCGCAACAACAGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                      <  6:768134/140‑1 (MQ=21)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAgcagcagca                                                                      >  2:852225/1‑140 (MQ=255)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGAAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTGACACAGCAGCCGCa                                                                      >  7:124668/1‑140 (MQ=255)
                                                                     cagccgcagGATGAGCAGGCGGAACCACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                      <  6:992743/140‑1 (MQ=37)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGt                                                                    <  5:461975/140‑1 (MQ=255)
                                                                                tCAGCAGCCGCAACAACCTGTTGCACCTCAGCCGCAGTATCAGCCGCCGCAATATCCACAGCCGCAACAACCTGTTGCACAGCAGCAGAAATATCAGCAGCCACAACAGCCGGTAACACAGCAGCCGCTGGAACagcagc                                                           >  7:445259/1‑140 (MQ=37)
                                                                                    cagcCGCAACACGCGGTTGCACCGCAGCTGTAGTAGAAGCAGGCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCa                                                       <  6:813086/140‑1 (MQ=21)
                                                                                      gcCGCAACAACCGGTTGCCCCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACACAGCCGCCGCAGTATCAGCAGACGCAAc                                                     >  3:1015246/1‑140 (MQ=37)
                                                                                      gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCCGCCCCCACTGCCGGTTAGCCAGCAGCCGCAGGAGCCGCCGCCGCAAc                                                     >  4:145880/1‑140 (MQ=25)
                                                                                      gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCCGCCACAACAGCCGGTTACGCAGCAGCCGCAGTATCAGCAGCCGCCAc                                                     >  2:152357/1‑140 (MQ=25)
                                                                                      gcCGCAACAACCGGTTGCACCGCAGCCGAAGTATCATCACCCGCAATATAAAAAGCCGCCACATCCATTTGCACCACAGCAGCAAAATCAGCCGCCGCTAAAGCCGGTGACACAGCTGCCGCAGTATCAGCAGCCGCAAc                                                     >  4:617428/1‑140 (MQ=21)
                                                                                       ccGCAACAACCGGGTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                                    <  3:510620/140‑1 (MQ=255)
                                                                                          caaccaCCGGACGCACCGCAGCAGCATCATCAGCATCCCCAAGATCAACATCCGCACCAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                                 <  6:408517/140‑1 (MQ=18)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAAACGGTAGCACCGCAGCAGCAAGATCAGCAGCCACAACAGCCGGGTACACAGCAGCCGCAGTCTCAGCAGCCGCACCAGcc                                                 >  3:791824/1‑140 (MQ=25)
                                                                                                       cACCGCAGCCGCAGTATCCGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  4:805164/140‑1 (MQ=255)
                                                                                                       cACCGCAGCAGAAGCCTCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCACCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  6:444364/140‑1 (MQ=21)
                                                                                                        cccgccgcagcagTATCAGCAGGCGCAATATCAACAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGc                                   <  3:305305/139‑1 (MQ=255)
                                                                                                         cctcagccgaatTCTCAGCAGCCGCAAGATCCACAGCCTCACCCACCGTTTGCACCGCAGGAGCAATCTCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  <  1:285871/137‑1 (MQ=18)
                                                                                                         ccgcagcagcagTCTCAGCAGCAGCACGATCACCAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGATACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  <  2:624653/140‑1 (MQ=18)
                                                                                                          ctcagccgaagTATAAGCAGCAGCAATATCAGCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTTCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  2:53979/138‑1 (MQ=17)
                                                                                                          cgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                         >  6:199075/1‑84 (MQ=255)
                                                                                                          cgcagcagaagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCATCAGCAATATCAGCAGCCACAACACCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  1:845024/140‑1 (MQ=255)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACTCCCGGTTGCCCCGCAGCAGCAATATCAGCCGCCACCACCGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTGGGACCACAGCCGcc                               >  5:351771/1‑139 (MQ=25)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACCCAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCCCcgccgcc                               >  8:237539/1‑139 (MQ=255)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGcc                               >  4:412376/1‑139 (MQ=255)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                               <  8:538401/140‑1 (MQ=255)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                               >  7:381349/1‑140 (MQ=255)
                                                                                                            cagccgcagGATCAGCAGCCTCAAGATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACACCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                               <  8:226452/140‑1 (MQ=18)
                                                                                                            cagcagcagTCTCTGCAGCAGCAATATCACCACCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                               <  6:58519/140‑1 (MQ=18)
                                                                                                              gccgcagTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                             <  1:319397/140‑1 (MQ=255)
                                                                                                              gcagcagTATCTGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCAGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                             <  2:30936/137‑1 (MQ=255)
                                                                                                                        cagcagCCTCAATATCATCAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt                   <  4:994402/140‑1 (MQ=255)
                                                                                                                          gcaggagCAAGATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTc                 <  5:253204/134‑1 (MQ=255)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACGCTGCGTCATc              >  8:313576/1‑140 (MQ=255)
                                                                                                                               cggaaTTTCATCAGCCGCAACAACCGTTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCTGTTACACAGCAGCCGCAGTTTCAGCAGCCTCAACTGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  7:138506/137‑1 (MQ=21)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGATCCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACATCCGCAGGAGACCCTGCTTCATCCg            >  6:945693/1‑140 (MQ=25)
                                                                                                                               agCATTATCACCAGCCCCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  6:178740/139‑1 (MQ=255)
                                                                                                                                tcattatCACCATCCTCATAAACCTGTTGCACTGCATCAGCAATATCAGCAGCAACAACAGCCGGGAACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGc           <  8:949728/136‑1 (MQ=18)
                                                                                                                                         aCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  8:133422/109‑1 (MQ=255)
                                                                                                                                         aCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGc  >  8:311515/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440197‑4440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A