Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F2 I0 R1 130 6907.4 7549744 98.4% 7428948 136.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 17.2% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC17.2% 129.2 / 4.0 123Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (38/50);  new base C (11/9);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

TGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTG  >  NZ_CP022686/4440196‑4440469
                                                                                                                                          |                                                                                                                                       
tGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCGCAATATCAAc                                                                                                                                        <  8:10915/140‑1 (MQ=255)
 gTACAGCAGCCGCAACAACCGGTAGCTCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACa                                                                                                                                       <  3:905198/140‑1 (MQ=255)
 gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTACACCGCAGCCGCAGAAACCGCAGCAGCAAAATCAGCa                                                                                                                                       >  5:629274/1‑140 (MQ=18)
 gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCTGTAACAGCAGCCGCAACAACCGCGTCCCCCGCAGCCGCCGTATCAGCAGACGCACTATCAACa                                                                                                                                       >  1:116702/1‑140 (MQ=18)
 gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGAAGCCGCAGTATCAGCAGCCGCAATATCAGCa                                                                                                                                       >  6:726596/1‑140 (MQ=255)
 gTACAGCAGCCGCAACAACAGGTATCACCGCAGCAGCAATATCAGCATCCGCAACAACCGGTAGCACAGCAGCCGCAGTATCAGCAGCCGCAACCACCGGTTCAACCGCAGCCGCAGGATCACGCGCCGCAATATCCAcg                                                                                                                                       >  7:852518/1‑139 (MQ=17)
   aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACATCCGGTTGCTCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                                     <  5:554872/140‑1 (MQ=255)
     agcagcCTCATCATCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCATCTTCCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCg                                                                                                                                   <  6:623703/140‑1 (MQ=21)
      gctgacGCAACAAACGGTAGCTCCGGAGGAGCAATATCTGCAGCCGCAAGATCCGGTAGAACCGCAGCCGCATTATCAGCAGCCGCAAGAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGc                                                                                                                                  <  4:502477/135‑1 (MQ=18)
       cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCCCAACAACCGGTAGCACCGCAGCAGCAGAATCAGCAGCCGCAAAAGCCGGATGAACCTCATCCGCAGTAGCAGCAGCAGCAAAAACAACAGCCGca                                                                                                                                 >  1:531082/1‑140 (MQ=17)
          ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCTCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                              <  2:121400/140‑1 (MQ=255)
          ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCATCAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                              <  2:22488/140‑1 (MQ=255)
          ccGCAACAACAGGTAGCACCGCAGCAGCAATATCAGCAGACGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                              <  3:299088/140‑1 (MQ=255)
           cGCACCAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTCTCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAACAGCCGCAAGATCAGCAGCCGcctcac                                                                                                                             >  7:490799/1‑135 (MQ=21)
            gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACATCCGGTATCACCGCAGCCGCAGGATCAGCAGCAGCAACAGCAGGTGGCTCCGCAGCCGCATTATCCGCCGCATCAAGAGCAACAGACGcaaacgc                                                                                                                            >  4:683813/1‑136 (MQ=17)
            gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCCGTTGCACCGCAGCCGCAGTATCAGCAGCCGCACTATCAACAGCCgggacacc                                                                                                                            >  7:787879/1‑133 (MQ=255)
               acaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCACTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCACCAGCCGCAACAACCgg                                                                                                                         >  5:879274/1‑140 (MQ=255)
                  acCGGTAGCCCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTg                                                                                                                      <  1:31433/140‑1 (MQ=255)
                        aGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgc                                                                                                                >  5:727645/1‑140 (MQ=255)
                           cccGCAGCAGAAATATCATCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGGATCAGCAGCCGCAACAACCTCTTGCACCGCAGCCGCAGTCTCTGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCgcagc                                                                                                             <  1:91981/139‑1 (MQ=21)
                            ccGCAGCAGCTTTATCAGCAGCCGCAACAACATGTATCACCGCAGCCGCAGTATCAGCAGCCTGATCAACCGGTTGCACCGCAGGTTGAGTATCAGGAGCCGCATTATCTACAGCCGCAACAACCGGTTGCACCgcagca                                                                                                            <  8:627515/140‑1 (MQ=21)
                              gcagcagcaCTATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTAGCAGCAGCCGCAACAACCGGTAGCACCGCAGCCTCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagc                                                                                                          <  5:407985/140‑1 (MQ=21)
                              gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCCGCCGCAGTATCAGCCGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGACGCAATATCAGCAGCCGCAAAACCCGGTTCGACCgcagcagc                                                                                                          >  7:490643/1‑140 (MQ=21)
                              gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCTCAGCCGCAGTATCAGCAGCCGCAATATCAACCGCCGCAACAACCGGTTGAACCgcagcagc                                                                                                          >  3:470483/1‑140 (MQ=255)
                               cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCCGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGACGCAACAACCGGTTGCACCgcagcagca                                                                                                         >  2:179153/1‑140 (MQ=255)
                               cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGAACCGCAGCCGCAGGATCACCAGCCGCAAAATCACCAGACGCAACATCCGGTTGCGCCgcagcagca                                                                                                         >  2:631551/1‑140 (MQ=21)
                               cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGTACCGCAGCCGCAGTATCAGCAGCGGCAAAATCACCAGCAGCCAAACCCCGATGCACCgcagaagca                                                                                                         >  1:871655/1‑140 (MQ=18)
                               cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagca                                                                                                         >  4:469097/1‑140 (MQ=255)
                               cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAACCGCAGCATCAGCAGCCGCAATATCACCAGCAGCAACAACCGGAGGCCCCtcagcagca                                                                                                         >  2:575941/1‑140 (MQ=18)
                                tgcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCaa                                                                                                        <  8:613070/139‑1 (MQ=255)
                                        tctCAGCAGCCGCAACAACCGGAAGCACCGCAGACGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                                <  5:911666/138‑1 (MQ=255)
                                        gatCACCAGCCGCAACAACCGGAAGCACCGCAGCCGCAGGATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                                <  4:599716/139‑1 (MQ=255)
                                          tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCTGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCGGCATGATCACCAGCCGCCCCAACCGGTTGCACCGCAGCAGCAATATCcgcagc                                                                                              >  3:299497/1‑140 (MQ=18)
                                          tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCATTATCTGCAGCCGAAACAACCGGTTGCTCCGCAGCCTCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGTTTGCACCGCAGCAGCAATATCagcagc                                                                                              <  7:261326/140‑1 (MQ=25)
                                            agcagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAACAGCCGCAACAACCGGTGGCACCGCAGCAGAAAGAGCAGAAGccc                                                                                            >  1:611682/1‑139 (MQ=18)
                                              cagcCGCACCAACCGGTAGCACAGCAGCCGCAGTATCAGCAGCCGCAACCACCGGTTGCACCGCATCAGCACGATCAGCAGCAGCATTATCACCAGAAGCCACGACCGGAGGCTCCGCAGCAGCAATCGcagccggcgca                                                                                          >  8:720986/1‑133 (MQ=16)
                                              cagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCAGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCACAATCAGCAACAGCCGGATGCACCTCAGCAGCAAAATCAGAAGCccca                                                                                          >  6:830033/1‑140 (MQ=17)
                                                 ccGGAACAACCTGTAGCTCCGCTGACGCAGTATCTGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  2:741503/140‑1 (MQ=25)
                                                 ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  1:118008/140‑1 (MQ=255)
                                                 ccGCAACAACCGGTAGATACGCAGCCTCATGATCAGCAGCCTCAACAACCTGTTGCACCGCAGCATCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  8:458419/140‑1 (MQ=25)
                                                  cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCGGCAGCAGCAAAATCAACAGCCGCAACAACCGGTGGCACAGCAGCAGCAATATCCGCAGCCACCACAg                                                                                      >  7:208707/1‑140 (MQ=21)
                                                  cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCAGCCACAGCCGGTAGCGCCGCAGAAGCAATATCAGCAGCCGCCACAg                                                                                      >  6:250265/1‑140 (MQ=18)
                                                   gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                     <  1:181417/140‑1 (MQ=255)
                                                         acCGGAAGAACCGCAGCCGCATGATCAGCAGCCGCAACCTCCGCATGCACCGCAGCCGCAGGATCAGCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTa                                                                               <  2:929324/140‑1 (MQ=25)
                                                             gTAGCTCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  5:128557/128‑1 (MQ=255)
                                                             gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       >  6:128557/1‑128 (MQ=255)
                                                               aGCACCGCAGACGCACTCTCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagc                                                                         <  4:504212/140‑1 (MQ=255)
                                                                   cctcagcagcagTATCAGCAGCCTCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  7:156610/137‑1 (MQ=255)
                                                                   ccgcatccgcagTATCAGCAGCCTGAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  6:369795/140‑1 (MQ=255)
                                                                   ccgcatcagcagTCTCAGCAGCCGCAACAACCTGTTGCACCGCAGACGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  4:884373/140‑1 (MQ=37)
                                                                   ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCATCAACAGCCGGTTCACCAGCAGcc                                                                     >  7:884877/1‑140 (MQ=37)
                                                                     gcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATCTCAGCAGCAGCAACAACCGGTTGCACCGCCGCAGCAATATCCGCCGCCACCACAGCCGGTTCCACAGCAGCCGc                                                                   >  2:592163/1‑140 (MQ=25)
                                                                      ccgccgcagTATCAGCAGCCGCAACCACCGCTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                  <  1:832331/138‑1 (MQ=255)
                                                                      cagccgcagTATCAGCAGCCGCATCAACAGGTAGCACCTCAGCCGCAGTATCAGCATCCGCTATATAATCACACGCAGCAACCGGTTTAACCGAAGAAGCAATATCAGCAGTCAACACAGACTGTTAATCAGAAGCCTCa                                                                  >  6:73527/1‑140 (MQ=255)
                                                                      cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCCGCAGCAATAGCGGCTGCCCCAACAGCCGGTTAGACAGCAGCCGcc                                                                  >  3:847002/1‑139 (MQ=37)
                                                                      cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                  >  1:665624/1‑140 (MQ=255)
                                                                        gccgcagTATCAGCAGCCGCAACAACCGGTTGAACCGCAGCCGCAGTATAAGCAGCCGCAAGATCATCAGCAGCAACAACCGGTTGCACCTCCGCTGCAAAATCAGCAGCCCCAACAGCCTGATAGCCAGCAGCCGCata                                                                >  5:830934/1‑138 (MQ=21)
                                                                            cagTATCAGCAGCCTCAACATCCGGTTTCACCTCAGCCTCATTATCAGCATCCGCAATATCAACAGCCGCTACATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATca                                                            <  8:787879/140‑1 (MQ=25)
                                                                            cagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATAAGCAGCCGCAAAATCAAAAGCAGCAACAACCGGAAGCACCGCAGAAGCAAAAACCGCAGCCACAACAGCCGTATAAAAAGAAGCAGCAGAATca                                                            >  4:590955/1‑140 (MQ=18)
                                                                              ttagaagcagcagcAACAACCTGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATTTCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATcagc                                                          <  3:509721/135‑1 (MQ=25)
                                                                                 tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGGATCAGCAGCCGCAAAATCAACAGCCGCAACCGCCGGTTGCACAGCAGCAGCATTATCAGCCGCACCAACAGCCGGGTACTCAGCAGCGGCAGGagcagcctc                                                       >  4:112541/1‑137 (MQ=21)
                                                                                   agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATTATCAACAGCCGCCACAACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGGATCAGCAGCCg                                                     >  1:423967/1‑140 (MQ=255)
                                                                                    gcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCCCCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAAACAGCAGCCGCAGTATCAGCAGCCGc                                                    >  1:621647/1‑140 (MQ=255)
                                                                                       gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACCGCCGGTTACACAGCAGCCGCAGGATCAGCAGCCGCAAc                                                 >  7:557365/1‑140 (MQ=255)
                                                                                        ccacaacaaCCCGTAGAACCGCTCCAGAAGTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACACCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                                <  8:119478/137‑1 (MQ=18)
                                                                                        ccGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGGATCAGCCGCCGCAACa                                                >  7:111464/1‑140 (MQ=37)
                                                                                         cGCAACAACCGGTTGCCCCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCATCAGCCGTGTACCCATCAGACGCTGGAGCCGCCGCCGCAAcgg                                               >  6:517934/1‑138 (MQ=21)
                                                                                         cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                               >  1:201195/1‑140 (MQ=255)
                                                                                         cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCCGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGAATCAGCAGCCGCAACAg                                               >  7:255189/1‑140 (MQ=255)
                                                                                           caacaaCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCCGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGGATCAGCAGCCGCAACAGcc                                             >  3:71989/1‑140 (MQ=255)
                                                                                              caacCGGTTGCACCGCATCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGTTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCATCAGCAGAATCAGCAGCCGCAACAGCCGGt                                          <  7:489463/140‑1 (MQ=25)
                                                                                                        cACCGCAGCAGCCGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                <  4:426038/140‑1 (MQ=255)
                                                                                                          ccgcatccgcagTCTCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                              <  8:77011/140‑1 (MQ=255)
                                                                                                          ccgcagccgcagGATCAGGAGCCTCAAGATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGGTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                              <  1:702593/140‑1 (MQ=21)
                                                                                                          ccgcagccgcagGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                              <  3:237253/140‑1 (MQ=255)
                                                                                                          ccgcagccgaagGATCAGCAGCCGCAATATCACCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                              <  2:799997/140‑1 (MQ=255)
                                                                                                           cgcagccgacgCATCTGCATGCACAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                             <  5:733240/140‑1 (MQ=18)
                                                                                                             catccgcagTATCAGCAGCAGCAAGATCAACAGCCTCAACAAACGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                           <  2:850855/137‑1 (MQ=21)
                                                                                                             cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                    >  6:64847/1‑99 (MQ=255)
                                                                                                             cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                    <  5:64847/99‑1 (MQ=255)
                                                                                                             cagccgcagAATCCCCAGACGCAATATGAAAAGACGCCACGACCGCTGGCACCTCAGGCGCATTATCCGCAGCCACAAACGACGGTTACACAGACGCCGCAGTAGCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                           <  5:526278/140‑1 (MQ=255)
                                                                                                               gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTAGCAGCAGCCGCAACATCCGGTTGTCCAACAGCCGCAgg                         >  5:432462/1‑140 (MQ=255)
                                                                                                                   cagTATCTGCAGCAGCAATATCAACACCCGCAACAACCGGCTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc                     <  5:913449/140‑1 (MQ=255)
                                                                                                                    aggatcatcAGCAGCAATATCACCAGCCGCCACACCCGGTTGCACCTCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                    <  3:64946/137‑1 (MQ=21)
                                                                                                                    agTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                    <  6:867800/140‑1 (MQ=255)
                                                                                                                     gTATCAGCAGCAGCAATATCAACAGCAGCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCTGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAccc                   <  1:290921/140‑1 (MQ=255)
                                                                                                                         cagcagCCGCAATTTCATCAGCCGCAACTCCCGGTTGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACCGCTGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt               <  7:262965/140‑1 (MQ=21)
                                                                                                                              gcagCAATCTCAACAGCCGCAACAACCTGTTGCACCTCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTAGAAGCAGACGCAACAGCAGGTTGTACAACAGCCGACTGATACCCTGCTTCATc          >  8:735861/4‑140 (MQ=21)
                                                                                                                                cGCATTATCAAGAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATAAGCAGCCACAACAGCCGGGTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        <  8:571238/140‑1 (MQ=21)
                                                                                                                                cGCAATATCAGCATCCGCAACAACCGGTGTCACCGCAGCAGCAATATCAGCAGCCTCAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        <  3:683813/140‑1 (MQ=18)
                                                                                                                                cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  2:523597/79‑1 (MQ=255)
                                                                                                                                cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                              <  1:789279/102‑1 (MQ=255)
                                                                                                                                cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        >  1:251639/1‑140 (MQ=255)
                                                                                                                                cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        >  4:667225/1‑140 (MQ=255)
                                                                                                                                cGCAATATCAACAGCCGCAACAACCAGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACACTGCTTCATCCg        >  6:351421/1‑140 (MQ=255)
                                                                                                                                  cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGcg      >  4:803792/1‑139 (MQ=255)
                                                                                                                                  cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCt      >  1:198257/1‑140 (MQ=255)
                                                                                                                                    atatCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt    >  6:658127/1‑140 (MQ=255)
                                                                                                                                     tatCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGtt   >  2:154404/1‑140 (MQ=255)
                                                                                                                                      atCAACAGCCTCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACATCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg  <  4:357764/140‑1 (MQ=25)
                                                                                                                                          |                                                                                                                                       
TGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTG  >  NZ_CP022686/4440196‑4440469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A