Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F2 I0 R1 184 7301.6 8720326 98.4% 8580800 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 13.5% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC13.5% 175.5 / 3.7 151Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (51/60);  new base C (4/16);  total (66/85)
Fisher's exact test for biased strand distribution p-value = 4.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

TACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440198‑4440473
                                                                                                                                        |                                                                                                                                           
tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCGGCAAAATcagcag                                                                                                                                          >  1:25184/1‑140 (MQ=18)
 aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                                         <  3:426503/140‑1 (MQ=255)
      agcCGCAACAACCGCTAGCTCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                                    <  7:430433/140‑1 (MQ=255)
        ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTAACAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACCGCCGcatcc                                                                                                                                  >  4:738155/1‑137 (MQ=255)
         cGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGTTGAACCTCAGCCGCAGAATCAGCAGCATCAATATCAAACGCAGcaac                                                                                                                                   >  5:645882/1‑138 (MQ=18)
          gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGAACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGGTACGCCACAGCCGAAGAAACAGCAGCAGCCAACTCCGCAGCCGCaacaac                                                                                                                                >  2:276770/1‑140 (MQ=17)
                   ggTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGAAGCCGCAGTATCAGCATCCTCAAAATCAGCAGCCGCAAAATCCggaggccc                                                                                                                       >  4:630821/1‑134 (MQ=18)
                       ccACCGCAGCAGCAATATCAGCAGCAGCAACAACCGGTAGAACCGCATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCCCAATATCAACAGCCGCAACAACCGGTTGCACCg                                                                                                                     <  6:221148/137‑1 (MQ=255)
                          ccGCAGGAGAAATATCATCAGCGGCAACAAGAGGTATCAGCGCATCCGCAGTATCAGCAGCCGCAACTACCGTTTGCACCGCAGCCTCAGTCACAGCAGGAGCAATTTCATCAGCAGCAACAACCGGTTGCACCgcag                                                                                                                  <  6:28072/138‑1 (MQ=16)
                           cGCAGCAGCAATAGCAGCAGCCGCAACAACCGGTAGCTCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGGAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCgcagc                                                                                                                 <  5:169822/138‑1 (MQ=255)
                             cagcagcaATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAACATCCGGTTGCCCCCCAGCCGCAGGATAACCAGCATCAGTATCATAATCCGCACCACCCGGAGGCACCactgcagga                                                                                                             >  7:241800/1‑138 (MQ=9)
                             cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCTCAGCCGCAGGATCAGCAGCATCAATATCAGCAGCAGCCACAACCCGATGCACCtcagcgg                                                                                                               >  6:589789/1‑136 (MQ=18)
                             cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGCTTGCACAgcagcagca                                                                                                             >  1:1131051/1‑140 (MQ=255)
                                   cattatCTGCAGCAGCATCAACCGGTAGCTGCGCAGCCGCATGCTCAGCAGCAGCAACATCCGGTTGCACCGCAGCCGCAGTCTCTGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCTGCAGCAATATca                                                                                                       <  4:1019982/137‑1 (MQ=21)
                                        tctgccgcCGCATCAAGCGGTAGCACCGCAGACGCAGTATCAGCAGCCTGATCTACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCagcagc                                                                                                  <  3:385043/134‑1 (MQ=25)
                                               ccGCATCAACCGGTAGCACCGCAGCCTCAGTATCAGCAGCCGCAACTACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  3:917731/140‑1 (MQ=255)
                                               ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  5:846061/138‑1 (MQ=255)
                                               ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCGCAAAATCAACAGCCGCCACAACCGGATCCCCGTCAGCAGCAAAATCAGCCGCCaccaca                                                                                           >  3:852426/1‑140 (MQ=18)
                                               ccGCAACAACAGGTAGCACCGCAGCCGAAGGAGCAGCAGCCGCAAGAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  6:936063/138‑1 (MQ=25)
                                                cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCAGCAGCATAATCAGCAGCAGCAACAGCCGGATGGACCGCAGCAGCAAAATCCGCCGCCCCAACAg                                                                                          >  3:938513/1‑140 (MQ=18)
                                                  caacaaCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGAAGCAGTATCAGCAGCCGCTAAATCAACAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCCGACACAACAgac                                                                                        >  2:413448/1‑138 (MQ=25)
                                                         cGGTAGCACCGCAGCCCCAGTATCAGCAGCCGCAACAACCCGTTGCCCCGCAGCCTCAGTATCAGCAGCCGCAATATCAACAGCAGCACCAACCGCTTGCAACGCAGATTCAATATCAGCAGCCACAACAGCCGGATaac                                                                                 >  8:270374/1‑138 (MQ=21)
                                                          tgTAGCACCGCAGCCGCATTATCAGCAGCCTCAACAACCTGATGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                                <  7:991688/139‑1 (MQ=37)
                                                          ggTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                                <  8:745674/140‑1 (MQ=255)
                                                            tcgatccgcagcagcctTATAAGCAGCAGACACAACCGGTTGCACCGCAGCCGCAGGATCATCTGCCCCATTATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcag                                                                              <  8:358771/135‑1 (MQ=21)
                                                            tAGAACCGCAGCCGAAGTATCAGCAGCCGCATCAACCCGATGCACCGCAGCCGCCGTCTCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                                <  6:71133/138‑1 (MQ=37)
                                                             atcACCGCAGCCGCAGTATCAGCAGCCGCAACTACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagc                                                                             <  7:404393/138‑1 (MQ=255)
                                                                aCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTCAACcgcagc                                                                          >  3:486567/1‑140 (MQ=37)
                                                                aCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACCGCCGGTTACACagcagc                                                                          >  1:32881/1‑140 (MQ=255)
                                                                aCCGCAGCCGAATGATCAGCAGCCGCATCATCCTGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagca                                                                            <  6:47767/138‑1 (MQ=25)
                                                                 ccgcatccgcatTATAAGCAGCCGCAATCACCTGATGCACCGCAGCCTCAGTATCAGCATCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  2:796965/140‑1 (MQ=25)
                                                                 ccgcagccgcagTCTCAGCAGCCTCAACAACCACTAGCACCGCAGCCGCAGTATCAGCAGCAGCATTATCATCAGCCGCAACAACCGGTAGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  1:885603/140‑1 (MQ=25)
                                                                 ccgcagcagcagTATCAGCAGCAGCAACAACCGGAAGCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagcag                                                                           <  5:1028906/138‑1 (MQ=37)
                                                                  cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  7:574657/140‑1 (MQ=255)
                                                                    cagccgcagTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCa                                                                      >  4:1090137/1‑140 (MQ=255)
                                                                    cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGACGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCAGGTTACACAGCAGCCGCa                                                                      >  7:178504/1‑140 (MQ=255)
                                                                    cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCATAAGCATCAACAACCGGTTTCACCGCAGCTGCAAGAACAGCAGCCCCAACAGCCGGTAACACAGCAGCCGCa                                                                      >  3:384529/1‑140 (MQ=25)
                                                                    cagccgcagGAGAAGCTGCCGGATGAACCGGATGCACCGCTGCCGCAGTCTCAGCAGCCTCAATATCACAAGCCTCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCAGGTTACACAGCAGCCGCa                                                                      <  4:503484/140‑1 (MQ=21)
                                                                    aagccgcagTATCAGCAGCCGCAACAACCGGTTGCACAGCAGCCGCAGTATCAGCAGCAGCAAAATCAACTGCAGCAAAAACCGCTGTCACCGCATCATCAATATCCGACTCCTCAACAGCCGGTTAAAcagcagaagca                                                                      >  2:669488/2‑135 (MQ=16)
                                                                      gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                      >  5:478746/1‑138 (MQ=255)
                                                                         gcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCCGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATc                                                                 >  7:254508/1‑140 (MQ=255)
                                                                                 tgcagcagcaTGATCCGGTAGCTCAGCAGCCGAAGGATCAGCAGCAGGAATATCAACAGCCGCAACAACCGTTTGCACCGCTGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCg                                                         <  4:791246/139‑1 (MQ=18)
                                                                                     gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAACAGCCGCAACAACCGGTTGCCCCGCAGCAGCAATATCACCAGCCACAACAGCCGGTTACACAGAAGCCGCAGTATCAGCCGCCGCAAc                                                     >  1:845995/1‑140 (MQ=38)
                                                                                       cGCAAGAACCTCCTGCACCGCAGCCGCAGGCTCAGCAGCCGCAATATCAACAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                                     <  6:587328/138‑1 (MQ=21)
                                                                                       cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCACGAGCAGCAACATCCGGTTGCACCGCAGCAGCAAAATCAGAAGACACAACAGCCGGTTCCACAGCAGCCGGAGAATAAGCAGCCGCAAAAg                                                   >  4:874244/1‑140 (MQ=25)
                                                                                       cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCCGCCACAACAGCCGGTTACACATCAGCCGCAGAATCAGCAGCCGCAACAg                                                   >  2:342752/1‑140 (MQ=255)
                                                                                       cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAAGATCAGCAACAACCGGATGCACCGCAGCAGCAAAATCAGCAGCCACAACAGCCGGTTAAACAGCAGCAGCAGAATCAGCAGCCGccaa                                                     >  5:289311/1‑135 (MQ=25)
                                                                                       cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           >  7:630545/1‑100 (MQ=255)
                                                                                       cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  8:630545/100‑1 (MQ=255)
                                                                                       cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCAGCAACAg                                                   >  1:175924/1‑140 (MQ=255)
                                                                                        gCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACGGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                                  <  7:716504/140‑1 (MQ=255)
                                                                                         caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                                 <  8:53921/140‑1 (MQ=255)
                                                                                         aaacttccGCTTGCTCCGCTGGCGCTTTCTCAGCAGGAGCAATATCAACAGCCGCAACTACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                   <  6:932939/132‑1 (MQ=17)
                                                                                            gctccGGATGCACCGCAGAAGCATGATTAGCAGCAGCAATATCAACATCCGCAACAACCGGCTGCACCGCAGCAGCATTATCAGCAGCCTCAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGt                                              <  8:372541/137‑1 (MQ=17)
                                                                                            caacCGTTTGCACCGCAGACGCATGATCATCAGCCTCAATATCAACAGCCGCAACAACCGGTTTCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCg                                                <  6:140069/138‑1 (MQ=21)
                                                                                                 ggTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAAAAGCCGCAACAACCGGTTGCACCGCAGAAGCAATATCAGCATCCACAACAGCAGGTTACACAGCAGCCGCAGTATCAGAAGCCGCAACAGCCGGTTGTAc                                         >  7:330770/1‑140 (MQ=39)
                                                                                                  gatGCACCGCAGCCGCAGGCTCAGCAGCAGCAAGCTCATCAGCCGCAACCACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACACCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTa                                          <  5:444515/136‑1 (MQ=18)
                                                                                                   ctcaaCCGCAGCCGCAGGATCAGCAGCAGCAATAACACCAGCCGCAACAACCGGATGCACCGCAGCAGCAATAGCAGCAGCCACAACATCCGGATACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACca                                       <  3:992946/136‑1 (MQ=18)
                                                                                                    tGCACCGCTGTGTCATTATCTGCAGCCTCATTATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                      <  2:705994/140‑1 (MQ=18)
                                                                                                      ctccgcagccgcagTATCTGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcctc                                      >  5:446120/3‑136 (MQ=255)
                                                                                                      cACCGCAGCCGCAGTATCATCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  7:84417/140‑1 (MQ=255)
                                                                                                      cACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGcc                                                                                                                                        <  6:338088/40‑1 (MQ=255)
                                                                                                      cACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGcc                                                                                                                                        >  5:338088/1‑40 (MQ=255)
                                                                                                      cACCGCAGCCGCAGGCTCAGCATCAGCAATATCAACAGGCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  8:240150/140‑1 (MQ=21)
                                                                                                      cACCGCAGCCGCAGGAGCAGCAGCATCAATATCACCAGCCGCCACAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  4:1082518/140‑1 (MQ=18)
                                                                                                        ccgcagcagcagTCTAAGCATGCGCCATATCACCAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  <  1:621682/140‑1 (MQ=18)
                                                                                                         cgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGTTTGTACCACAGCCg                                 >  1:291463/1‑140 (MQ=255)
                                                                                                          gcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGcc                                  >  6:203386/1‑138 (MQ=255)
                                                                                                           cagccgcagTCTCAGCCGCAGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                               <  7:323773/140‑1 (MQ=255)
                                                                                                           cagccgcagTATCTGCAGCAGCAATATCAACAGCCGCAACAAGCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                               <  1:474891/140‑1 (MQ=255)
                                                                                                           cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGcc                               >  4:525894/1‑139 (MQ=255)
                                                                                                           cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTCTACCACAGCCGcc                               >  4:855697/1‑139 (MQ=255)
                                                                                                           cagccgcagCATCAGCAGCCGCAATATCAACATCCGCATCAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCTCATCAGCCGTTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  5:353065/138‑1 (MQ=21)
                                                                                                           cagcagcagTATCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGCTGCACCGCAGCAGCAATATCAGCAGCCACAACACCCGGCTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  5:452688/138‑1 (MQ=21)
                                                                                                             gccgcagTATCATCATCCTCAATTTCCACCGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCCGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                             <  1:1016677/140‑1 (MQ=18)
                                                                                                             gccgcagTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                             <  7:21613/140‑1 (MQ=255)
                                                                                                             cactcagTATCATCAGCAGCTAATTCAACAGCCGCCTCAACAGGTTGCACCGCAGCATCATTATCATCAGCCACAACAGCCTGGTACACAGCAGCCGCAGTATCAGCATCCGCAACAGACGGTTGTACCACAGCCGCAgg                             <  8:80973/136‑1 (MQ=255)
                                                                                                              ccgcacTCTCTGCAGCAGCAAGATCCACACCCGCCACAACAGCCTGCACAGCAGCATCAATATCAGCAGCCACAACATCCGGTAACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGa                            <  3:694533/140‑1 (MQ=17)
                                                                                                               cgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCTGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCCgg                             >  5:177060/1‑138 (MQ=255)
                                                                                                               cgcagTATCAGCAGCCGCAATATCAACAGCCGAAACAACCGGTTGAACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAAACAGCAGCCGCAGTATCAGCAGCCGCACCAGCCGGTAGTACCACAGCCGCAgg                             >  6:367218/1‑138 (MQ=25)
                                                                                                                  atgctCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  7:948412/136‑1 (MQ=255)
                                                                                                                  agTATCATCAGCATCAATATCAACAGCCGCAACAACAGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  5:1042341/138‑1 (MQ=255)
                                                                                                                  agTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  6:303370/138‑1 (MQ=255)
                                                                                                                      tCAGCAGCCGCAATATCAACAGCCGCAACATCCGGTTGCACCGCAGCATCATTATCAGCAGCAACGACAGCCGGTTACACAGCAGCCGCAGAATCAGCAGCCGCAACAGCCGGATCTAGCACAGCCGCAGGATACCATGc                    >  8:760782/1‑140 (MQ=255)
                                                                                                                            gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATCTCCGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGATGTACCACAGCCGCAGGCTACCCTGCtgcctc              >  4:683826/1‑135 (MQ=21)
                                                                                                                            gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              >  3:1044079/1‑140 (MQ=255)
                                                                                                                            gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              >  1:31146/1‑140 (MQ=255)
                                                                                                                            gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCAGCAGCCACAACAGCCGGTTACACATCAGCCGCAGTATCAGCAGCCGCAACAGACGGTTGTACCACAGCCGCAGTATACCCTGCTTCATc              >  1:664001/1‑140 (MQ=255)
                                                                                                                             ccGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATcc             <  7:381188/140‑1 (MQ=255)
                                                                                                                              ctcAATATCACCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  3:653968/138‑1 (MQ=255)
                                                                                                                              cGCAATATCAACAGCCGCAACACCCGGTTGCACAGCAGCAGCAATATCATCAGCCACAACCTCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  2:853348/140‑1 (MQ=25)
                                                                                                                              cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAcaca                                                                               <  5:990431/73‑1 (MQ=255)
                                                                                                                              cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAcaca                                                                               >  6:990431/1‑73 (MQ=255)
                                                                                                                              cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                                 >  2:75821/1‑103 (MQ=255)
                                                                                                                              cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                                 <  1:75821/103‑1 (MQ=255)
                                                                                                                               gCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACATCCGCAGGATa                          >  6:642873/1‑125 (MQ=255)
                                                                                                                               gCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  5:642873/125‑1 (MQ=255)
                                                                                                                                cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  6:15475/1‑138 (MQ=255)
                                                                                                                                    atCAACAGCCGCAACAACAGGTTGCAACGCAGCAGCAATATCAGCATCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt        <  5:1022059/138‑1 (MQ=255)
                                                                                                                                        aCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACACTCCTTCATCCGCTGATGATGc  >  2:1130220/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440198‑4440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A