Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F2 I0 R1 130 5456.6 6845820 98.4% 6736286 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 15.9% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC15.9% 129.8 / 2.0 120Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (33/50);  new base C (7/11);  total (51/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

CTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTG  >  NZ_CP022686/4440195‑4440469
                                                                                                                                           |                                                                                                                                       
cTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCaa                                                                                                                                         <  2:736259/140‑1 (MQ=255)
   tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTCAACCTCAGCCGCAGTATCCGCAGCCGCAAAATcagcag                                                                                                                                      >  1:481261/1‑140 (MQ=18)
          gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGTTGAACCACAGCCGCAGTATCAGCAGCCGCAAAAGCTAGAGCAGcaaa                                                                                                                               >  3:329923/1‑139 (MQ=18)
           ccGCATCAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCATCTACCGGAAGGACCGCAGGCGCAGTATCAGCTGCCGCTACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCTATATCAACAGCCGcaact                                                                                                                              <  8:444526/140‑2 (MQ=25)
           ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGAACCTCAGCCTCAGTATCAGCAGCCGCAACAACCGGATCATCCGCAGCCGCAGTATCAGCAGCCGCAAAATCACAAGACGccaca                                                                                                                              >  3:176794/1‑140 (MQ=18)
             gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCTCAGCCGCAGGAGCAGCAGCAGCAATCTCCGAAGACGCcacaac                                                                                                                            >  6:543170/1‑140 (MQ=14)
             gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTCCACAGCAGCCGCAGTATCAGCAGCCGCAAAAACCGGTTGCACCGCAGCCGCAGGATCCCCAGCAGCAAAAGCAGCAGCCGCaacaac                                                                                                                            >  2:47985/1‑140 (MQ=14)
             gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGACGCaacaac                                                                                                                            >  1:361966/1‑140 (MQ=255)
                   acCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTg                                                                                                                      <  1:563895/140‑1 (MQ=255)
                       gTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAGCAGACGCAACATCCGGTTGCAcc                                                                                                                  >  8:353745/1‑140 (MQ=21)
                          gCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                               <  5:670063/140‑1 (MQ=255)
                          ccACCGCAGCAGCAATATCAGCAGCAGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                               <  3:44178/139‑1 (MQ=255)
                            cgcGCAGCAGCAATATCAGCAGCAGCAACAACCGGTAGCACCGCAGCCGCAGTATCTGCAGCCGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                             <  7:770058/138‑1 (MQ=21)
                            aCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                             <  8:319355/140‑1 (MQ=255)
                             ccGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTAGCAGCAGCCGCAACAACCGGTTGCACCGCAGCCTCAGTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagca                                                                                                            <  6:408719/140‑1 (MQ=255)
                              cGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTAACCGCAGCCGCAAAATCAGAAGCAGCCACAGCCGGATACACCgaagcag                                                                                                           >  4:219329/1‑140 (MQ=18)
                               gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACAGGTTGCACCGCAGCCGAAGTATCAGCAGCCGCAAAATCACCAGCCGCAACAACCGGTTGCACCgcagcagc                                                                                                          >  4:581355/1‑140 (MQ=255)
                                cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATAAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCATTATCAGCAGCCGCAATATCAACTGCCGCAACAACCGGTTGCCCCtcagcagcc                                                                                                         >  7:488064/1‑139 (MQ=21)
                                        ttatCAGCAGCAGCAACAAACGTTAGAACCGCATCCGCATTATCATCAGCATCAACACCCTGTTGCACCGCAGGCGCAGTATCAGGAGCCGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagc                                                                                                 <  8:788299/139‑1 (MQ=25)
                                        atatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGAAGCCGCAATATCAACAGCCGCAACAACCGGTTTCACCGCAGCAGCAATATcagc                                                                                                 >  7:11454/1‑140 (MQ=255)
                                         tatCAGCAGCCGCAACAACAGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGAATCATCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                                <  3:43667/140‑1 (MQ=255)
                                         cctCAGCAGCCGCATGAACCTGTATCACCGCATCAGCAGGCTCAGCAGCAGCATCAACCGGATGCACCGCAGCCGCAGTATCATCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                                <  2:545573/138‑1 (MQ=21)
                                               cagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCcaca                                                                                          >  7:172624/1‑140 (MQ=255)
                                               cagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCcaca                                                                                          <  2:725252/140‑1 (MQ=255)
                                                agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAACTGCCGCAACACCCGGTTGCCCCGCAGCAGCAAAATCAGCAGCCccaa                                                                                         >  3:221096/1‑140 (MQ=21)
                                                 gcCGCAACACCCGGTAGAACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGACCCGCAGCCGCAGTAGCAGCAGCCGCAATATCAGAAGCCGCAACAACCGGGTGCACCTCAGCAGCAAAATCAGCAGCCccaac                                                                                        >  6:230262/1‑140 (MQ=37)
                                                 gcCGCAACAACCGGTAGCACCGCCGCCGCAGGATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCGGCAAAATCAGCCGCCGCCACAGCCGGGTGCCCCGCAGCAGCATAATCAGCAGcccccac                                                                                        >  8:423958/1‑135 (MQ=18)
                                                 gcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCGCCGGTTGCACCGCAGCCGCAGTAGCCGCCGCAGCAAAATCCGCAGCCGCCACAGCCGGATCGCCCGCAGGCGCATAATCAGAAGCcacccc                                                                                        >  4:652420/1‑137 (MQ=9)
                                                 gcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATACGCAGCCGCATGAGCAAGAGCAGCAACGGCAGGTTGACCCGCAGCAGCAATAAAAGCAGcaacaaa                                                                                        >  4:63153/1‑139 (MQ=17)
                                                 gcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCGGCAAAATCAACAGCCGCCACAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCccaac                                                                                        >  5:87418/1‑140 (MQ=21)
                                                 gcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGACGAAACAACCGGATACACAGCCGCAGCATTATCAGCAGCAccaac                                                                                        >  1:372358/1‑140 (MQ=18)
                                                  ccGCAACAACCTGTAGCACCGCAGCAGAATTATCTGCAGCCGCAACATCCTGTTGCACCGCAGCCGCAGTATCAGCAGCATCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  4:848129/140‑1 (MQ=21)
                                                  ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGATGCACCGCAGCAGCAATATCAGCAGACacaaca                                                                                       >  7:480253/1‑140 (MQ=255)
                                                  ccGCAACAACCGGTAGCACCGCAGACGCATGATCAGCAGCCGGAACCTCCGGTTGCACCGCAGCATAAGGATCAGGAGCCGCAATATCCACAGCCGCAACAACCGTTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  8:374618/140‑1 (MQ=25)
                                                  cagCAACAACATGTAGCTCCGCATCCGCAGTATCAGCAGCCGCAACATCCTGTTGCACCGCAGCCGAAGTATCAGCAGCATCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                       <  8:686835/138‑1 (MQ=37)
                                                   cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCTCAGCCGCAGTATCCGCAGCAGCAAAATCAGCAGCCGCAACATCCGGATGCACCTCAGCAGCATAATCAGCAGACGCAACAg                                                                                      >  8:341425/1‑140 (MQ=18)
                                                    gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCATCAGCCGCAACACCCGGATGCACCGCAGCAGCAAAATCCGCAGCCCCAACAGc                                                                                     >  8:651006/1‑140 (MQ=21)
                                                    gCAACAACCGGTAGCACCGCAGCCGCAGTAGCAGCAGCCGCAACCCCCGGGGGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                     <  1:791055/140‑1 (MQ=37)
                                                            ctgTAGAACCGCAGCCGCAGGATCAGCAGCCGCATCAACCGCATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTaca                                                                             <  4:268804/138‑1 (MQ=37)
                                                            aggTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTaca                                                                             <  7:58318/139‑1 (MQ=255)
                                                             ggTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCTCAGTATCAGCTGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                            <  7:262577/140‑1 (MQ=255)
                                                              gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCACCAGCCGCAACAGCCGGTTGCACCGCAGCAGCATTATCAGCAGCCGCAACAGCCGGTTAcaca                                                                           >  2:800295/1‑140 (MQ=25)
                                                               aaGCACCGCAGCCGCATTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACCGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcag                                                                          <  6:554543/139‑1 (MQ=255)
                                                                   agcgctgcagcagTATATGCATCATCTACAACCGGTTGCACCGCAGCCTCAGTCTCTTGAGGATCAATATCACCCGCGGCAACAACCGGTTGAACGGCATCAGCATTATCAGCAGCCACTACAGCCGGTTACACagcagc                                                                      <  6:620469/138‑1 (MQ=255)
                                                                   aCCGCATCCGAATGATCAGCAGGATCAACTACCTGCTGCACCGCAGGAGCCGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                      <  8:478307/140‑1 (MQ=21)
                                                                   aCCGCAGCAGCAGTCTCAGCAGCCGGATCAACCTGATTCACCGCTGCCGCAGTATCAGCAGCCGCAATTTCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                      <  2:375977/140‑1 (MQ=25)
                                                                   aCCGCAGACGCATGAGAAGCAGGCGCAACAACCTCATGCACCGCAGCAGCAGTCTCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                      <  2:375975/140‑1 (MQ=18)
                                                                    ccgcagccgccgTAGCAGCAGCAGAAACAACCGCTTGCACCGCAGCCTCAGTCTCTGCAGCCGCAATATCAACAGCCGCAACAACCGGTTTCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  8:474965/140‑1 (MQ=37)
                                                                    ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  4:425335/140‑1 (MQ=255)
                                                                    ccgcagccgcagGATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCATCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  8:563477/140‑1 (MQ=255)
                                                                    ccgcagcagcagGAGCATCAGCCTGAACAACAGGACGCACCGCAGAAGCAGTATCAGCAGCATCATTATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  2:112264/140‑1 (MQ=21)
                                                                     cgcagccgcagTCTCTGCAGCCGCATCAACCGGATGCACCGCAGCCGCAGTATCAGCAGCATCAAGATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                    <  7:378048/140‑1 (MQ=37)
                                                                       cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                  <  1:616137/140‑1 (MQ=255)
                                                                       aagccgcagTCTCTGCAGCAGCAACAACATCTTGAAGAGAATACTCAGTATCAGCAGGATCAATATCAACAGCATCAACAACCTGTTTCACAGGAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACATCAGCCGCa                                                                  <  4:308473/139‑1 (MQ=255)
                                                                               gTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                                <  1:545512/70‑1 (MQ=255)
                                                                               gTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                                >  2:545512/1‑70 (MQ=255)
                                                                                 ctCAGCAGCAGCAACATCCTGATGCACCGCAGCCGAAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAAGAGCCGGTTACACAGCAGCCGCAGTATcagcag                                                        <  1:171446/139‑1 (MQ=21)
                                                                                  tCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCCCCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCagcagc                                                       >  3:364725/1‑140 (MQ=255)
                                                                                    agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAAACAGCAGCCGCAGTATCAGCAGccc                                                     >  6:791194/1‑139 (MQ=255)
                                                                                       cgcagCAACCACCGGATGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                                  <  1:246543/136‑1 (MQ=255)
                                                                                       agcTGCAACATCCGGTTGCTCCGCAGCCTCATTATCAGCAGCAGCAATAGCAAGAGCCGCATCATCCTTTAGCACCGCAGCAGCAATATCAGCAGACACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                                  <  7:676769/140‑1 (MQ=18)
                                                                                         ccGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCAGCAGCCACCACAGCCGGATCCGCAGCAGCCGCAGGCTCCGCCGCCGCAACa                                                >  4:188921/1‑140 (MQ=37)
                                                                                          cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGACGCCGCCACAGCCGGTTACACATCAGCCGCAGTATCCGCAGCCGCAACAg                                               >  2:606471/1‑140 (MQ=25)
                                                                                          cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     >  6:454233/1‑118 (MQ=255)
                                                                                          cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTGACACAGCAGCCGCAGTATCAGCCGCCGCAACAg                                               >  3:46983/1‑140 (MQ=255)
                                                                                          cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                     <  5:454233/118‑1 (MQ=255)
                                                                                          cGCAACAACCGGTTGCACCGCAGACGCAGTATCAGTAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAAGATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                               >  7:465228/1‑140 (MQ=255)
                                                                                            catgaacCCGATGCACCGCAGCCGCAGTCTCAGCAGCAGCATTATCAACAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                             <  4:536720/136‑1 (MQ=18)
                                                                                            cacgacccGGATGCCCCGCAGCCGCAGCCTCAGCAGCCGCAAGATCAACAGCCGCCACAACCTGTTGCACCGCAGCAGCAATATCAGCAGCCACACCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                             <  4:474666/134‑1 (MQ=21)
                                                                                               caacCGGATGCACCGCAGCCGCAGAATACGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCCGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGt                                          <  6:276362/140‑1 (MQ=21)
                                                                                                    ggTTGCACCGCAGCCGCAGTCTAAGCAGCCGCAAGATCAACATCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAc                                     <  3:10371/140‑1 (MQ=21)
                                                                                                           ccgcagccgcatTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                              <  7:169566/140‑1 (MQ=255)
                                                                                                           ccgcagccgcagTCTCAGCAGCATCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                              <  1:849653/140‑1 (MQ=255)
                                                                                                           ccgcagcagtagTATCTGCCGCCGGAATTACATCAGGCGGAACATCCGGTTGCACCGCAGCAGCAATATCAGCAGGCAAAACAGCAGGTTTCACAGCAGCAGCAGTATCAGCAGCCTCAACAGCCGGTTGTACCACAGcc                              <  3:229382/140‑1 (MQ=17)
                                                                                                            cgcagccgaagGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                             <  3:525116/140‑1 (MQ=255)
                                                                                                              cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                           >  1:55792/1‑140 (MQ=255)
                                                                                                               agccgcagTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGCCGCAg                          >  2:305232/1‑140 (MQ=255)
                                                                                                               agccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACATCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAg                          >  6:81718/1‑140 (MQ=255)
                                                                                                                gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTAACAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                         >  3:177797/1‑140 (MQ=255)
                                                                                                                    aagTATCAGCATCCGCATTATCATCAGCCGCAACAACCTGTTGCAGCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAGAGCAGCCTCAGTATCAGCAGCCGCAACAGCCGGTTGTACAACAGCCGCAGGATAc                     <  3:581585/139‑1 (MQ=21)
                                                                                                                     agTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGTACCACAGCCGCAGGATAcc                    <  5:1746/140‑1 (MQ=255)
                                                                                                                     agTATCACCACCAGCAATTTCAACAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                    <  7:513135/140‑1 (MQ=21)
                                                                                                                     agTATAAGCAGTCGGAATTTCATCAGCCGCAACAACAGTTTGCACCGCATCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTGTACCTCAGCCGCAGGATAcc                    <  5:1744/140‑1 (MQ=18)
                                                                                                                         tCAGCAGCCGCAATATCAACAGCCTCACCAACCGGCTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTAACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCTCAGGATACCCTGc                <  6:546105/140‑1 (MQ=21)
                                                                                                                           agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGCTTGTACCACAGCCGCAGGATACCCTGCtt              >  5:71778/1‑140 (MQ=255)
                                                                                                                               ggcGCAAGATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCACTATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc          <  2:371378/138‑1 (MQ=18)
                                                                                                                               gcctgattatCTACAGCCTCCACAACCGGCTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc          <  6:683185/133‑1 (MQ=21)
                                                                                                                               gcagCAATCTCACCAGCCGCAACAACTGGTTGCACCGCATCAGCAATATCAGCAGCCACAACAGCCGGTTACACTGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc          <  5:157703/137‑1 (MQ=21)
                                                                                                                               gcCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc          <  7:236923/140‑1 (MQ=255)
                                                                                                                                 cGCAATATCAACAGCCGCAACAACCGGTTGCACCg                                                                                                                 >  1:185058/1‑35 (MQ=255)
                                                                                                                                 cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        >  3:529832/1‑140 (MQ=255)
                                                                                                                                 cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        >  5:731829/1‑140 (MQ=255)
                                                                                                                                 cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATACg        >  4:232226/1‑140 (MQ=255)
                                                                                                                                 cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGATTCCTCCg        >  3:830968/1‑140 (MQ=255)
                                                                                                                                 cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGAACCACAGCCGCAGGATCCCCAGCTTCCTCCg        >  2:78965/1‑140 (MQ=255)
                                                                                                                                 agCAATATCATCAGCCGCAACAACCGGTTTCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg        <  3:63153/139‑1 (MQ=255)
                                                                                                                                  gCATTATCAACAGCCGCAACAACCGGATGCACCGCATCAGCAATATCAGCAGCCACAACACCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGc       <  2:112647/140‑1 (MQ=21)
                                                                                                                                  gCAAGATCACCAGCCGCAACAACCGGTTGCACCGCAGAATCAATATCACCAGCCTCAACAGCCGGTTTCACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGc       <  8:665170/140‑1 (MQ=37)
                                                                                                                                   cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGcg      >  7:81441/1‑139 (MQ=255)
                                                                                                                                       atcatcAGCCGCAACAACCGGTTTCACAGCAGCAGCAATATCAGCAGCCACAACACCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg  <  4:329923/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTG  >  NZ_CP022686/4440195‑4440469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A