Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F35 I0 R1 275 15706.7 10805118 98.3% 10621430 134.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 16.9% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC16.9% 205.9 / 4.7 189Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (57/68);  new base C (8/21);  total (87/102)
Fisher's exact test for biased strand distribution p-value = 9.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

ACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440199‑4440473
                                                                                                                                       |                                                                                                                                            
ccagcagCCGCAAGATCCGGTAGCTCCGCAGCAGCACTATCAGCAGCCGCAACAACCGGTAGCACAGCAGCCGCAGTATCAGCAGCCTCAACCACCGGTGGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACa                                                                                                                                            <  4:848197/137‑1 (MQ=18)
aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCCGCCGCAGGAAAAGCGGCAGCAAGATCACCa                                                                                                                                            >  3:104592/1‑138 (MQ=14)
 cagcagCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTCCACCGCAGCCTCAGTATCAGCAGCCGCAATATCACCAg                                                                                                                                           >  1:22065/1‑138 (MQ=255)
    cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAAAAACCGGTTGAACCGCAGCCGCAGGATCCGCAGCAGCATAATCAGCAGACGcc                                                                                                                                     >  7:40416/1‑139 (MQ=17)
      gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCTCAGCCGCAGTATCCGCAGCAGCAAAATCAGCAGACGcc                                                                                                                                     >  2:1222481/1‑137 (MQ=18)
      gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTGAAACCAAAGCCGCAGTATCAGCAGCAGCAATATCAGAAGACGcaac                                                                                                                                   >  8:375686/1‑140 (MQ=18)
       ccGCAACAACCGGTAGCACCGCAGCAGCCATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCCGCCGcaa                                                                                                                                    >  5:418225/1‑138 (MQ=21)
       ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGcctgtcgg                                                                                                                                  >  7:1235629/1‑134 (MQ=255)
       ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAACATCCGGTTTATCCACAGCCGCAGTATCCTCAGCAGCAAAATCAAAAGCCGccaca                                                                                                                                  >  8:1286385/1‑140 (MQ=17)
        cGCAACAACCGGTAGCACCTCAGCAGCAATATCACCAGCCTCAACCCCCGGTAGCACCGCAGACGCAGAATCAGCAGCCGCAACAACCGCTTCCACCCCATCCGCATTAACAGCAGCCGCAATATCAAAAGCCGcaac                                                                                                                                   >  3:1133588/1‑138 (MQ=21)
        cGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCTCCGCAGCCGCAGTATCCACAGCAGCAAAATCAGCAGCCGcaac                                                                                                                                   >  2:225738/1‑138 (MQ=17)
          caccaacCGGTAGCACCGCAGCAGCAATCTCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaacaa                                                                                                                                 <  1:338363/135‑1 (MQ=255)
           aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTGCACCACAGCCGCAGTATAAGCAGCAGCAACATCCGGTTGGACATCATCCGCAGAGACAGCAGCGGCATAATCAGCAGCCGcaacata                                                                                                                                >  6:803875/1‑136 (MQ=9)
           aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCTCAACAACCGGTAGCACCGCCGCCGCAGTATCAGCAGCCGCAACACACGGTTGCACCGCAGCCGCAGTAGCAGCAGCAGCAATATCCACAGCCGcaacatc                                                                                                                                >  4:1107550/1‑136 (MQ=21)
           aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAACACCCGGTTCAACCGCAGCCGCAGGCTCAGCAGCAGCATAATCAGCAGCCGccacagc                                                                                                                                >  2:667304/1‑136 (MQ=17)
           aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCTCAGCCGCAGGATCAGCAGCAGCAATATCACCAGCCGccacagc                                                                                                                                >  4:880765/1‑136 (MQ=18)
            actaaaggTAGCTCCGCATCAGCAATATCAGCAGCAGCAACAACCTGTAGCACCGCATCCGCAGTATCTGAAGCCTCAACAACCGGTTGCACCGCAGCCGCAGTATCATCAGCAGCAATATCAACAGCCGCAACAAcc                                                                                                                               <  2:719773/132‑1 (MQ=21)
             caacCGGTAGCACCGCAGCAGCAATATTAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTAGCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCg                                                                                                                              <  5:1254795/138‑1 (MQ=255)
             caacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCg                                                                                                                              <  5:282378/138‑1 (MQ=255)
               acCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGt                                                                                                                            <  1:10540/138‑1 (MQ=255)
                     aGCTCCGCCGGAGCATTATCAGCCGCCTCATCTACATGTATCTCCGCATCAGAAGTCTCAGCAGCAGCATCATCCGGTAGCACCGCAGCATCAGTCTCAGGAGCCTCATTATCATCAGCCGCAACAACCGGTTGCAcc                                                                                                                      <  2:132709/138‑1 (MQ=255)
                      ccACCGCAGCAGCAAAATCACCAGCCGCAACAACCGGTAGCACCGCCGACGCAGTATCAGCAGCCGCAACATCCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCgca                                                                                                                   <  7:1287683/139‑1 (MQ=21)
                       cACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTCCACACAAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGACCCACCgc                                                                                                                    >  1:27743/1‑138 (MQ=21)
                        cccGCAGCAGCAATCTCAGCAGCAGCATCAACCGGTAGCACCGCATCCGCAGTATCAGCAGCAGCAACCACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCgca                                                                                                                   <  2:901158/137‑1 (MQ=21)
                          cGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCACCCGCAACAACCGGTGAAACCGCATCCGCAGAATCATCCGCCGCAAAATCAACAGACGCAACAACAGGATGAGCCtcagcag                                                                                                               >  8:620677/1‑140 (MQ=18)
                           gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagca                                                                                                                <  5:1313732/138‑1 (MQ=255)
                            cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCAGCAGCCGCAAAAGCCGGTAGCACCgccgcagca                                                                                                             >  8:655782/1‑140 (MQ=18)
                              gcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCCGCAATATCAACAGCCGCAACAACCGGTTGCCCCgcagcagca                                                                                                             >  4:906983/1‑138 (MQ=255)
                              gcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagca                                                                                                             >  4:1148889/1‑138 (MQ=255)
                              gcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGACGCAGTATCCGCAGCCGCAATATCACCAGCCGCCACAACCGGTTGGACCgcagcagca                                                                                                             >  2:496664/1‑138 (MQ=21)
                              gcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCCCAACAACCGGTTCAACCGCAGCCGCAGAAGCATCATCAGCAATATACCCAGCACGCACAACCGGATTAACAgcagcggca                                                                                                             >  6:679918/1‑138 (MQ=12)
                              gcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGGATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCCAAAGCACCAGCCGCAACAGCCGGTGGCGCCgccgcagcc                                                                                                             >  5:563893/1‑137 (MQ=18)
                                       tCAGCAGCAGCATCAACCGGTAGCACCGCATCAGCATTATCAGCAGCCTCAACAACATGATGCACCGCAGCCGCAGTATCAGCTGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                                    <  6:501007/138‑1 (MQ=21)
                                         tgcctccGCAACAAGCTGTAGCAACGCTGCAGCAGTCTAAGCAGGAGCATCATCCTGTTGCACCGCAGTCTCAGTATCAGCTGGCGCAATATCAACAGCCGCAACAACAGGTTGCACCGCAGCAGCAATATCagcagc                                                                                                  <  6:1116161/133‑1 (MQ=19)
                                         agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGAACCGCAGCCGCAGTATCCGCAGCCGCAAAATCAGCAGCCGCCACATCCGGATGCACCTCAGCAGCAATATCcgcagc                                                                                                  >  4:780003/1‑138 (MQ=18)
                                            cgcgggaacaaaCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCACCGGATGCACCGCAGCCGCAGTCTCAGCAGCCGCAATCTCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCcac                                                                                               <  4:737408/132‑1 (MQ=37)
                                            cgcCGCATCAACCTGAAGCACCGCATCCGCAGTAGCAGCAGCCGCAACCACCGCTTGCACCGCAGCCGCAGTATCATCAGCCGCAATAACACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCcac                                                                                               <  2:1258663/137‑1 (MQ=25)
                                            agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCcac                                                                                               <  1:5487/138‑1 (MQ=255)
                                            agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAgccgcccc                                                                                               >  6:391718/1‑136 (MQ=255)
                                            agcCGCAACAACCGGTAGAACCGCAGCCTCAGTATAAGCATCCGCAACAACCGGTTGAACCGCAGACGCAGTATCCGCGGAATCAAAATCAACATCAGCAAAACCAGGGAGCTCCACAGCAGCAAAAACCGCAGcccc                                                                                               >  4:31675/1‑136 (MQ=9)
                                              ccGCAACAACCTGAAGCAGCGCAGCAGCAGTCTCAGCAGCCGCAACTACCGGTTGCACCGCAGCCGCAGTATCAGCATCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  8:440217/140‑1 (MQ=25)
                                              caggatcaacCGGAAGCACCGCAGCCGAATGATCAGCAGCAGCAACAACCTGAAGCACCGCAGCCTCAGTAACAGCAGCCTCAATATCACCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  6:1169161/132‑1 (MQ=21)
                                               cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAGAAGCCGCAACAACCGGATGCACCGCAGAAGCAATATCAGCCGCGGCAACAg                                                                                          >  8:540068/1‑140 (MQ=18)
                                                   acaacCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCCCAGTATCAGCAGCCGCAATATCAACAGACGCAACACCCGGTAGCACCGCCGCAGCAATATCAGCAGCCACCACAGCCgg                                                                                      >  7:606317/1‑140 (MQ=25)
                                                   acaaaCGGAAGAACAGCAGCCGCAGTCTCAGCAGCATCATCAACCGCTTGCACCGCAGAATAAGAATCATCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCgg                                                                                      <  8:1026517/140‑1 (MQ=18)
                                                               gccgcagccgcagTATCAGGAGCCGCAACAACCTGTTGCACCTCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACTGCCGGTTACAcagca                                                                            <  1:10239/137‑1 (MQ=38)
                                                                ccgcatccgcagGAGCAGCAGCCTCAACAACCTGTGGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagcag                                                                           <  6:463714/138‑1 (MQ=37)
                                                                ccgcagccgcacTCTCAGCAGCCGCAACCACCGGATGAACCGCAGACGCAGTATCAGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagcag                                                                           <  4:338580/138‑1 (MQ=37)
                                                                ccgcagcagcctGATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATAAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagcag                                                                           <  5:517905/138‑1 (MQ=37)
                                                                ccgcagcagcacTCTCAGCTGCAGCAACAACCGCAAGCACCGCAGCCTCAGCCTCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagcag                                                                           <  2:900426/138‑1 (MQ=18)
                                                                cagcagcagaagTAGCAGCATCCTCAAATACAGGTTGCACAGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCTCAACAACCGGTTGCACCGCATCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGAAGcc                                                                         <  8:1039128/138‑1 (MQ=25)
                                                                 cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                          <  4:686466/138‑1 (MQ=255)
                                                                 cgcaccagcagTAGCAGCAGCAGCAACTACCGCTTGCACCGCAGCCTCAGGCACAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                          <  4:143729/138‑1 (MQ=25)
                                                                     gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCACAACCGCCGGTTACCCAGCAGCCGcc                                                                      >  6:227847/1‑137 (MQ=255)
                                                                            tATCAGCAGCAGCAACAACCGGACGCACCGCAGCCTCAGTCTCAGCATCAGCATTATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATcag                                                               <  4:1023685/138‑1 (MQ=21)
                                                                             aTCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGGCACAACAGCCGGTTACACAGCAGCCGCAGTATcagc                                                              >  2:10239/1‑138 (MQ=255)
                                                                                     ccGCACCATCCGGTTGCACCGCAGCCGCAGTCTCAGCAGCCGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCACCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                                      <  1:863862/138‑1 (MQ=25)
                                                                                     ccGCAACAACCTGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                                      <  5:806707/138‑1 (MQ=255)
                                                                                      cccaccaacCCGTTGCACACCTCCCGCATTTTCAGCAGCAGCAAAATCTACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                                     <  5:34754/133‑1 (MQ=17)
                                                                                      cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGAGGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTACCCCGCAGCAGCAGTAGCAGCCGCCGCAAc                                                     >  4:1072499/1‑138 (MQ=18)
                                                                                      cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACACAGCAGCCGCAGTATCAGCCGCCGCAAc                                                     >  1:1224863/1‑138 (MQ=255)
                                                                                       gCAAGAACCGGATGCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                                  <  8:164606/140‑1 (MQ=255)
                                                                                        caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGAAGCAGCAGAATCAGCCGCCGCAACAg                                                   >  1:513037/1‑138 (MQ=37)
                                                                                        caacaaCCGGTTGAACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCAGCAACATCCGGTTGCACCGCAGCAGCAATAACAGCAGCCACCACAGCCGGTTACGCATCAGCCGCAGAATCCGCAGCCCCAACAg                                                   >  6:739333/1‑138 (MQ=37)
                                                                                           caacCGGAAGCACCGCAGCCGCAGTATCAGCAGGCTCAATATCTACAGCCGCCACAACCTGTTGCAACTCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCg                                                <  6:410561/138‑1 (MQ=21)
                                                                                           caacAGGTTGCACCGCAGCCGCAGTATAAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGt                                              <  8:100323/140‑1 (MQ=255)
                                                                                                  atGCACAGCAGCCTCATTATAAGCAGCAGCAAGATCATCAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCCTCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAc                                         <  2:565163/137‑1 (MQ=18)
                                                                                                  aagaaCCGCAGCCTCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAc                                         <  1:1391417/134‑1 (MQ=255)
                                                                                                   cgCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCAACACCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcc                                        <  5:97412/137‑1 (MQ=255)
                                                                                                   agCACCGCAGCCGCAGCCTCAGCAGCCGCAAGATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcc                                        <  2:377490/137‑1 (MQ=255)
                                                                                                       ccgctgcagcagTATCAGCAGGAGCAAGATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  <  7:887969/140‑1 (MQ=18)
                                                                                                       ccgcagccgcagTATCAGCTGCAGCAATATCACCAGCCGCAACACCAGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  6:1234316/138‑1 (MQ=21)
                                                                                                       ccgcagccgcagTATCAGCAGCCGCAAGATCAACACCAGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  1:1317421/138‑1 (MQ=21)
                                                                                                        cgcagacgcagTCTCAGCAGGCGCAATATCAACAGCCGCAACAACCGGCTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGc                                   <  3:579681/138‑1 (MQ=255)
                                                                                                            gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACGGCCGGTGACACAGCAGCCGCAGTATCAGCAGCAGCAGAAGCCGGTTGTACCACAGCAGCAgg                             >  8:175483/1‑140 (MQ=21)
                                                                                                                 atgctCAGCAGCCGCAATTTCAACAGCCGCAACAACCGCTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  3:95715/134‑1 (MQ=255)
                                                                                                                  gTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc                         >  2:1170962/1‑138 (MQ=255)
                                                                                                                      cagcagCCGCAATATCAACAGCCGCAACTACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt                   <  7:1084086/140‑1 (MQ=255)
                                                                                                                       agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTgg                    >  3:34210/1‑137 (MQ=255)
                                                                                                                        gcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCTGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTTAACCACCGCCGCAGGATACCCTGcg                   >  3:375198/1‑137 (MQ=21)
                                                                                                                         cagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt                  >  5:410963/1‑138 (MQ=255)
                                                                                                                           gcagCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa                <  3:1311529/135‑1 (MQ=255)
                                                                                                                           gcCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa                <  2:414928/138‑1 (MQ=255)
                                                                                                                           gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa                <  5:381831/138‑1 (MQ=255)
                                                                                                                           gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              >  7:1136201/1‑140 (MQ=255)
                                                                                                                           gcCGCAAGATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              <  7:116449/140‑1 (MQ=255)
                                                                                                                            cagCAATATCAACAGCCGCAACAACCGGTTTCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATcc             <  8:295591/138‑1 (MQ=255)
                                                                                                                             cGCTATATCACCAGCCTCAACACCCGCTTGCACAGCATCATCAATATCAGCAGCCACAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGACGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  8:888791/140‑1 (MQ=21)
                                                                                                                             cGCAATATCATCAGCAGCAACATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              <  6:572374/138‑1 (MQ=255)
                                                                                                                             cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              >  5:1051462/1‑138 (MQ=255)
                                                                                                                               cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACACCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  6:153764/1‑138 (MQ=255)
                                                                                                                               cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                      >  2:321029/1‑112 (MQ=255)
                                                                                                                               cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATAAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  4:477811/1‑138 (MQ=255)
                                                                                                                                 atatCACCAGCCGCAACAACCTGTTGCACCGCCGCAGCAATATCAGCAGCCTCAACACCCGGTAACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCAGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt        <  8:823322/140‑1 (MQ=18)
                                                                                                                                   ttCAACAGCCTCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACTGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt        <  3:895700/137‑1 (MQ=255)
                                                                                                                                   atcatcAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg      <  8:219570/140‑1 (MQ=255)
                                                                                                                                   atCAACAGCCGCAACAACCGGTTGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg      <  7:189863/140‑1 (MQ=255)
                                                                                                                                   atCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCATCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGt        <  2:708176/138‑1 (MQ=255)
                                                                                                                                      aaCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATg   >  7:669328/1‑140 (MQ=255)
                                                                                                                                       aCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCCGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGc  >  7:941720/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                            
ACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440199‑4440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A