Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F35 I0 R1 271 8871.0 10354886 98.4% 10189207 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 23.5% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC23.5% 103.1 / 8.9 131Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (46/33);  new base C (9/15);  total (70/61)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440199‑4440473
                                                                                                                                       |                                                                                                                                           
aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTCAGCCTCAGCCGCAGGATCCGCAGCAGCAAAagcagcagc                                                                                                                                         >  4:159514/1‑140 (MQ=14)
aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCAGCAGCAAGATCAACAGc                                                                                                                                         >  1:331173/1‑140 (MQ=18)
    cagcagAAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCTCAGCCGCAGTCTCAGCAGCAGCACAACCCGGTTTAACCGCAGCCGCAGGATAAGCAGCCGCCAACGCATCAGCCGcc                                                                                                                                     >  6:410398/1‑139 (MQ=17)
    cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAACAGCCGGTTGTACCTCATCCGCAGTATCAGCAGCAGCAACAGCAGCAGACGcc                                                                                                                                     >  4:1188119/1‑139 (MQ=14)
    cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAAAAACCGGTTGCTCCGCAGCCGCAGTATCCTCAGCAGCAATATCAGCAGcctcc                                                                                                                                     >  7:397749/1‑137 (MQ=18)
    cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTTCACCGCAGCCGGAGTAACTGCAGCCGCAATCTCAACcgccgcc                                                                                                                                     >  7:747047/1‑139 (MQ=18)
       ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTAACCCGCAGCCGCAGTATCAGCAGCAGCAAAATCACCagcagccccc                                                                                                                                  >  5:901958/1‑136 (MQ=18)
       ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGAACCGCAGCCGCAGGATCCGCAGCCGCAAAATCAAGAGCCGcaaca                                                                                                                                  >  1:143030/1‑140 (MQ=18)
       ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                  <  2:1287333/140‑1 (MQ=255)
       ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAAAGGCCGcaaaa                                                                                                                                  >  7:1028234/1‑138 (MQ=21)
         gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCCGCCGCAACAACCGGTAGAACCGCAGCCGCAGTATCAGCAGACGCAACAACCGGTTTCACCGCAGCCGCAGTATCAGCAGCGGCAAGATCAACAGCCGCaacaac                                                                                                                                >  4:236705/1‑140 (MQ=25)
         gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTGAACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCTCAGCCGCAGTAGCCGCAGCAGCATAATCAGCAGCAGccacagc                                                                                                                                >  4:976676/1‑138 (MQ=14)
         gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAACAACCGGTTTCCCCTCAGCCGCAGTATCATCAGCATCAAAAACAGCAGACGcaacagc                                                                                                                                >  6:250244/1‑138 (MQ=17)
         gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTATCACCGCAGCCGCAGTATCAGCAGCCGCAACAAACGGAGGAACCTCAGCCGCAGTATCAGCCGCCGCAATATCCCCAGACGCcaccac                                                                                                                                >  7:339773/1‑140 (MQ=18)
         gCAACAACCGGTAGCACATCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACAGCAGCCGCAGTATCAGCTGCAGCAACAACCGGTTGCACCTCAGCCGAAGTATCAGCAGCAGCACAATCACCAGCCGcaacatc                                                                                                                                >  2:594932/1‑138 (MQ=18)
                      ccACCGCAGCAGAAATATCAGCAGCCGCAACAACCTGAAGCACCGCATCCGCACGAGCAGCAGCCGCAACAACCTGTAGCACCGCAGCCGCCGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCgca                                                                                                                   <  1:406931/139‑1 (MQ=21)
                       cACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCAGCAAAATCATCTGCCGCTACAACCGGAAGCACCgcag                                                                                                                  >  3:50195/1‑140 (MQ=18)
                         ccGGAGGAGCAAGATGAGCAGCCGCATCAAGCTGTAGCACCGCAGCGGAAGTCTCTTCAGCCGCAACTTCCTCTTGCACCGCATCCGCAGTATCAGCAGGCGCAATATCACCAGCCGCAACAACCGGTTGCACCgcagca                                                                                                                <  8:310766/140‑1 (MQ=16)
                          cGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCTCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTAGCAcagcagcag                                                                                                               >  1:549439/1‑140 (MQ=21)
                           gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAGCCGGTTGCACCgcagcagc                                                                                                              >  3:649928/1‑140 (MQ=255)
                            cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTAGCACCgcagcacca                                                                                                             >  8:885156/1‑140 (MQ=255)
                            cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGACGCAACATCCGGTTGCCCCgcagcagca                                                                                                             >  8:881209/1‑140 (MQ=255)
                                    agagctgcagcCGCATCATCCTGTAGCTCCGCATCCGCAGTATCAGCAGCCGCATGTTCCGGAGGCACCGCAGCCGCAGTCTCTGCTGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagc                                                                                                     <  7:1068244/134‑1 (MQ=21)
                                       tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCagcagc                                                                                                  <  8:39942/140‑1 (MQ=255)
                                            agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCAGCAGCCGCAACACCCGGTGCCACCGCAGCAGCAAAATCCGCAGccccca                                                                                             >  3:323920/1‑136 (MQ=18)
                                            agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             >  6:361864/1‑140 (MQ=255)
                                            agcCGCAACAAACTGTAGCTACGCATCAGCATTATCAGCAGCCGCATCTACCGGTTGCACCGCAGCCTCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  7:1010346/140‑1 (MQ=21)
                                              ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  4:1092758/140‑1 (MQ=255)
                                                 caacaaCCGGTAGCACCGCAGCCGCAGTATCAGCAGCTGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCCCAGCAGCAGCAATATCAGCAGCCACAACCGcc                                                                                        >  5:557819/1‑140 (MQ=25)
                                                 caacaaCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGcc                                                                                        <  7:1089401/140‑1 (MQ=255)
                                                   agaacTGGTAGGACCGCATCCGAACTATCTGCAGCAGCATGTACCGGATGAACCGCAGCCGAAGTATCTGCAGGAGCAATATCATCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCgg                                                                                      <  3:320558/138‑1 (MQ=12)
                                                         ggaaGCACCGCATCCGCAGTATCAGCAGCCGCATCAACCGCATGCACCGCAGCCGCAGTATCATCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                                <  5:901756/137‑1 (MQ=37)
                                                          gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATAAGCAGCCGCATAAACACCAGCCGCACCATCCGGTTTCACCGCAGCAGCAATATCAGAAGACCCAACAGCCGGATTcaca                                                                               >  6:1071027/1‑140 (MQ=18)
                                                           tAGCAGCGCAGCCGAATTCTCTGCAGCAGCATCTACCGGTTGCACCGCAGACGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcag                                                                              <  2:510573/140‑1 (MQ=25)
                                                            aGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAAACGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCTGTTGAACCGCAGCAGCAATATCAGCAGCGACAACAGCCGGTTACAcagc                                                                             >  3:603778/1‑140 (MQ=37)
                                                             ccACCGCACCCGCAGGATCAGCAGCCGCAACAACCGGCAGCACCGCAGCCGCAGTATCAGCAGCCCCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCATCAATATCAGCCGCCACAACAGCCGGTTACAcagca                                                                            <  3:572554/139‑1 (MQ=37)
                                                                ccgccgccgaatGATCTGCAGCAGCAACCACCGCAGGCACCGCAGCAGCAGTATCAGCAGCATCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  2:1002901/140‑1 (MQ=21)
                                                                ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         >  1:258798/1‑140 (MQ=255)
                                                                ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCACCAACCGGAAGCACCGCCGCAGCAATATCAGCGGCCACAACAGCCGGTTACACAGCAGcc                                                                         >  3:261525/1‑140 (MQ=37)
                                                                ccgcagcagcatGATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGAATCAGCATCATCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  2:955135/140‑1 (MQ=25)
                                                                ccgcacccgaagTCTAAGCAGCCTCAACTACCTGACGAACCGCAGCCTACGTCTAAGCATCCTCAATATCACCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  6:241034/140‑1 (MQ=12)
                                                                 cgcctcagcagGATCAGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  1:786152/133‑1 (MQ=37)
                                                                 cgcagctgcagGCTCAGCAGGCGGAAGATCCGCTAGCACCGCAGCCGCAGCATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  3:249521/140‑1 (MQ=25)
                                                                 cgcagccgaagTATCATCAGCAGCAACAACCGGTTCCACCGGATCCGCAGAATCAGCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  6:624791/140‑1 (MQ=25)
                                                                   ccgccgcagTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCCGCAGCGGCAATAGCAGGTGCCGCCCCATCCGGGGGCCCGGCAGGGGCAACATCAGCCGCCACCACAGCCGGATAGCCATCagcagcc                                                                      >  8:1159071/3‑139 (MQ=12)
                                                                   cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCCGCAGACGCAACAGCCGGATACACAGCAGCCGCa                                                                      >  3:1258069/1‑140 (MQ=37)
                                                                   cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACACAGCAGCCGCa                                                                      >  1:1267641/1‑140 (MQ=255)
                                                                   cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCCGCAGCCGCAACAGCACGAGCCGCCTCATCCGGAGCCACCGCAGCAGCAAAATCAGAAGCCACCCCAGCCGGATAACCatcagcgtaa                                                                      >  6:1026550/1‑136 (MQ=12)
                                                                            gagcagcagCCGCAAAAACCGTTTGCAGCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATcagca                                                             <  2:769618/137‑1 (MQ=25)
                                                                                agcagcCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTGTCACAGCAGCCGCAGGATCAGCAGccc                                                         >  3:331322/1‑139 (MQ=25)
                                                                                agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCCCCGCCGCAGCAATATCCGCAGCCACCACAGCCGGTTACCCAGCAGCCGCAGAATCAGccgccg                                                         >  2:752473/1‑140 (MQ=37)
                                                                                agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATAGCAGCAGCCCCAACAGCCGGTTACCCAGCAGCCGCAGAATCAGCAGCCg                                                         >  7:1228888/1‑140 (MQ=255)
                                                                                  cagcCGCAACAACCGGTTGCACCGCAGCAGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCa                                                       <  6:593483/140‑1 (MQ=255)
                                                                                     ccGCAACCACCGGATGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                                    <  1:550723/140‑1 (MQ=255)
                                                                                      cGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATAATCAACATCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCACGTTACACAGCAGCCGCAGTATCAGCATCCGCAACAg                                                   >  3:280913/1‑140 (MQ=25)
                                                                                      cGCAACAACCTGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCACCTGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCTGCCACAGCGGCCGGGTACGCAGCCGACGCGGTGTCAGCAGCCGCAAcgg                                                   >  1:1003147/1‑138 (MQ=25)
                                                                                      cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTGGCACCGCAGCAGCAAAATCCGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCCGCAGCCGCAACAg                                                   >  2:1125218/1‑140 (MQ=25)
                                                                                      cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGTTTACACAGCAGCCGCAGTATCCGCAGCCGCAACAg                                                   >  2:1320813/1‑140 (MQ=255)
                                                                                      cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTCCCCAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                   >  7:525602/1‑140 (MQ=255)
                                                                                      cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCCGCAGCAGTATCAGCAGCCGCAACAg                                                   >  4:641648/1‑140 (MQ=255)
                                                                                      cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACAGGTTGCACCGCAGCAGCAATATCCGCAGCCGCAACAGCCGGTTAAACCGCAGCAGCAGTATCAGCAGCCGACACAg                                                   >  4:517118/1‑140 (MQ=21)
                                                                                       gCAACACCCTCTTGCACCGCAGCCGCAGGCTCTGCAGCCGCAAGCTCAACAGCCGCCACACCCGGTTTCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTCTCACTGCAGCCGCAGTATCAGCAGCCGCAACAGc                                                  <  7:77672/140‑1 (MQ=18)
                                                                                        caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           >  8:718937/1‑98 (MQ=255)
                                                                                        caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCCGCAGCCGCAACAGcc                                                 >  1:769618/1‑140 (MQ=255)
                                                                                                  ttGCACCGCAGCTTCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                                       <  5:199587/140‑1 (MQ=255)
                                                                                                  ttGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                                       <  4:624620/140‑1 (MQ=255)
                                                                                                   tGCACCGCATCCGCAGTATAAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACGGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                      <  5:321137/140‑1 (MQ=21)
                                                                                                   tGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACTCTGCAGCCGCAGTATCAGCAGCCGCACCAGCCGGTTGTACcac                                      >  5:517679/1‑140 (MQ=255)
                                                                                                   tGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                      <  5:621590/140‑1 (MQ=255)
                                                                                                     acccgcagccgcagTCTCAGCAGCCGCAATATCAGCAGCCGCAACCACCGGTTGCACCGCAGCAGCAATCTCAGCAGCCCCAACAGCCGGTTCCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  3:1165677/138‑1 (MQ=21)
                                                                                                       ccgcatccgcatGATCTGCAGCCGCAAGATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  <  6:1161464/140‑1 (MQ=21)
                                                                                                       ccgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  >  3:561912/1‑140 (MQ=255)
                                                                                                       ccgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                  >  1:929748/1‑140 (MQ=255)
                                                                                                        cccagcctcagGCTCAGAAGCCCCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  8:339773/138‑1 (MQ=18)
                                                                                                         gcatccgcatTCTCAGCATCATCAATATCAACAGACGCATCAACCGGTTGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGc                                <  5:1144182/140‑1 (MQ=18)
                                                                                                          cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCTATATCAGCAGCCACAACCGCCGGTTACGCAGCAGCCGCAGTCTCAGCAGCCGCAACAGCCGGTGGTACCACAGCCGcc                               >  8:192086/1‑139 (MQ=38)
                                                                                                          cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGGACCACAGCCGCa                               >  6:182229/1‑140 (MQ=255)
                                                                                                                 aggaTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  8:1090593/137‑1 (MQ=255)
                                                                                                                 agaaTCAGCAGCCGCAATATCAACAGCCGCAACAACCTGTTGCTCCGCAGGAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  1:1298544/137‑1 (MQ=255)
                                                                                                                 agTCTCTGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCTCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  4:223546/140‑1 (MQ=21)
                                                                                                                 agTCTCAGCAGCCGCATTCTCACCAGCCGCACCATCCGGTTGCAACGCAGCAGCAATATCAGCAGCCACACCAGGGGGGTACACAGCAGCCGCAGTATCAGCCGCCGCAACAGCCGGTTGTACCACCGCCGCAGGATAcc                        <  7:1008112/140‑1 (MQ=21)
                                                                                                                 agTCTCAGCAGCATCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  1:1174445/140‑1 (MQ=255)
                                                                                                                 agTATCAGCAGACGCATTATCATCAGCCGCAACACCCTGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCTCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  1:563110/140‑1 (MQ=21)
                                                                                                                     tCAGCAGCCGCAATATCAACCGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGc                    >  3:469052/1‑140 (MQ=255)
                                                                                                                      cagcagcagCAATATCACCAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt                   <  1:1180035/140‑1 (MQ=255)
                                                                                                                          agcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAGATCAGCAGCCACCACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTcct               >  5:1126136/1‑138 (MQ=255)
                                                                                                                           ggagaaaTATCAACAGCCGAATCTTCCTGTTGCACCGCATCAGCAATATCAGCAGCCACAACATCCTGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGACGCAGGATACCCTGCTTCATc              <  8:737941/135‑1 (MQ=21)
                                                                                                                           gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCATCCTCAACACCCGATTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGATCTTCAACAGCCGCAGGATACCCAGCTTAAta              >  4:236844/1‑139 (MQ=18)
                                                                                                                           gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc              >  5:478007/1‑140 (MQ=255)
                                                                                                                           cacGCAATATCAACAGCCGCAACAAACTTTTTCACCGCATCAGCAATATCAGCAGCCACAACAGCCGTTTACACAGCAGCCCCACAAAAAAAAGCCGCAACAGCCCGAGGAACCAAAGCCACACAAAACCAAGCTTCaaa              >  4:590828/3‑138 (MQ=255)
                                                                                                                             ctcAATATCAACAGCCGCAACAACCGGTAGCACCGCAGAAGCAATATCAGCAGCCACAACAGCCGTTTACCCAGCAGCCGAAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACACTCCTTCATCCg            >  7:539393/3‑140 (MQ=18)
                                                                                                                             cGCATTATCCTCAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGAAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  2:1252018/140‑1 (MQ=21)
                                                                                                                             cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  3:392618/1‑140 (MQ=255)
                                                                                                                             cGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  8:478066/140‑1 (MQ=255)
                                                                                                                             agCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCAGCAGCCACAACAGCAGGTTTCACAGCAGCCGCACTATCAGAAGACTCAACACCCGGTTGTACCACAGCCGCAGGAAACCCTGCTtcctccc            >  4:519970/2‑139 (MQ=37)
                                                                                                                               cAATATCAACAGCCGCAACAACCGGTTGCCCCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATCCCCTGCTTCATCCGcg          >  3:752785/1‑139 (MQ=255)
                                                                                                                                   ttCACCAGGCGCATCAACAGTTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTTCACTGCAGCAGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg      <  7:1212486/139‑1 (MQ=21)
                                                                                                                                   atCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg      <  2:270960/140‑1 (MQ=255)
                                                                                                                                      aaCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCCGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATg   >  6:489850/1‑140 (MQ=255)
                                                                                                                                       aCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCAGCTTCATCCGCTGTTGATGc  >  6:928639/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
ACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440199‑4440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A