Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,479,619 T→A 15.7% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,479,6190TA15.7% 416.4 / 10.4 197intergenic (‑28/+165)ftsB/ygbEcell division protein FtsB/DUF3561 family protein
Reads supporting (aligned to +/- strand):  ref base T (107/47);  new base A (2/20);  total (113/84)
Fisher's exact test for biased strand distribution p-value = 6.24e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

CGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGATTGAATT  >  NZ_CP022686/1479481‑1479756
                                                                                                                                           |                                                                                                                                         
cgccACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATc                                                                                                                                          <  2:551828/140‑1 (MQ=255)
cgccACATCAATATTGCCGCGAGTATAGTCATGTATACCGTTCTTTCCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACATCAGCGTTAGTTTACCC‑ATCCTGCCACATGAAAAACGGCATCTTCATc                                                                                                                                          <  8:987177/140‑1 (MQ=255)
     aatcttTATTGACGCGAGTATTGTCAATTATACCGGTCTTAGCGAACCAGAGCGAATCCTGTAGCCAGACCAGAATAGCCAGCTAGTGCAGCGTTAGTTTACCG‑ATCGAGCGCCCTGAAATACTGCATCTTCATCCCATg                                                                                                                                     <  2:687501/139‑1 (MQ=255)
        aattattGACTCTAGTATAGTCATGTAAACCGTTCTTACCGAACCACAGCGAATAATGTAGCCAGACCACAATAGCCACCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCAt                                                                                                                                  <  7:117909/139‑1 (MQ=255)
                aCGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑TTCCTGCCCCCTGAAAAACGGCTTCTTCATCCCAGGCATCCGAAgac                                                                                                                          >  2:869796/1‑140 (MQ=255)
                  gcgAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAgacgc                                                                                                                        >  5:214548/1‑139 (MQ=255)
                  gcgAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAgacga                                                                                                                        >  3:489919/1‑140 (MQ=255)
                     aGTATAGTCATGTATACCGTTCTTACCGAACCACATCGAATACTGTAGCCAGACCAGAAAAGCCAGCAACAGCAGCGTTAGATTACCC‑ATCCTGCCCCCTGAAAAACGGCATCATCATCCCATGCATCCGAAGACGActc                                                                                                                     >  4:934169/1‑140 (MQ=255)
                     aGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGActc                                                                                                                     >  7:258960/1‑140 (MQ=255)
                           tacATTTATCCCGTTGTTACCGAACCACCCCTAATACTGTCGCCATACCAGAATTGCCCGCAACCGCCGCGTTAGTTTACCC‑ATCCGGCCCCCTGAAAACCGGCATCTTCACCCCATGCATCCGAAGACGACTCTACATc                                                                                                               <  6:979805/138‑1 (MQ=255)
                           gtaaTGTATACCGTTCTTACCGAACCACCCCTAAAACTGTACCAAGCCACTAAAACCCATCAACAGAAGCTTTTGTTTACCC‑ATCCTGCCCCCTTAAAAACGGAATATTCATCCCATGCATCCTAAGACGACTCTACATc                                                                                                               <  4:1034533/137‑1 (MQ=255)
                           gTCTTGTATACCGTTCTTCCCGAACCACTCGGAATAGTGTTGCCAGACCAGAATAGCCAGCAAAACCAGCGTTTGTTTACCC‑ATCCTGCCCCCTGAAAAACGCCATCTTCCTCCCATGCACCCGAACACGACTCTACATc                                                                                                               <  8:852079/140‑1 (MQ=255)
                             cATGTATACCGTTATTACATAACCACCGCGAATACTTTAGCCAGACCATAAAACCCCGCAACATCAGCGTTAGTTTACCC‑ATCCTCCCCCCTTAAAACCGTCATCTTAATCCAATGCTACCTAAGACGACTCTACATCct                                                                                                             <  4:814789/140‑1 (MQ=255)
                              aTGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCCTCCCCTGCATCCGAAGACGACTCTACATCctc                                                                                                            >  7:621818/1‑140 (MQ=255)
                                gTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTg                                                                                                          >  6:492124/1‑140 (MQ=255)
                                gTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCAACCGAAGACGACTCTACATCCACTg                                                                                                          >  7:375399/1‑140 (MQ=255)
                                 tatcCCGTTATTACCCAACCACAGCCAATACTGACGCCACACCACAATCCCCCGCAACCGCAGCGTTAGTTAACCC‑ATCCTGCCCCCTCAAAACCGGCATCTTCAACCCATGCATCCCACGACGACTCTACATCCTCTGt                                                                                                         <  8:295437/140‑1 (MQ=255)
                                 tataCCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGCCTCTACATCCTCTGt                                                                                                         >  6:464831/1‑140 (MQ=255)
                                 tataCCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTCCATCCTCTGt                                                                                                         >  6:811302/1‑140 (MQ=255)
                                    aCCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTgg                                                                                                      >  2:33028/1‑140 (MQ=255)
                                      cGTTCTTACCGAACCACAGCGAATACTGTAGCCAGCCCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTgggg                                                                                                    >  5:775623/1‑140 (MQ=255)
                                      cGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTgggg                                                                                                    >  8:437051/1‑140 (MQ=255)
                                      cGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTgggg                                                                                                    >  6:396324/1‑140 (MQ=255)
                                      cGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTgggg                                                                                                    >  1:322663/1‑140 (MQ=255)
                                      cGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACAACCTCTGTTgggg                                                                                                    >  4:301188/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTATCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCAGCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  6:683946/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  1:689326/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  3:95932/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  8:643110/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  8:57748/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  6:584469/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCAGCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  1:904506/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAATTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  4:848545/1‑140 (MQ=255)
                                        ttCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGGTAGTTTCCCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCTTGCATCAGAAGACGACTCTACATCCTCTGTTGGGGAt                                                                                                  >  6:236039/1‑140 (MQ=255)
                                          cTTACCTACCCACAGCTAATACTGTAGCCACCCCACAAACCCCACCAACCGCAGCGTTAGTTAACCC‑ACCCTGCCCCCTCAAAAACGGAATCTTCACCCCATGCATCCGAACACGACTCTACATCCTCTGTTGGGGATAc                                                                                                <  2:757481/140‑1 (MQ=255)
                                          cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGCCGACTCTACATCCTCTGTTGGGGATAc                                                                                                >  1:1020580/1‑140 (MQ=255)
                                          cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATAc                                                                                                >  7:206257/1‑140 (MQ=255)
                                          cTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATAc                                                                                                >  5:114267/1‑140 (MQ=255)
                                          cTTACAGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCAGCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATAc                                                                                                >  4:603913/1‑140 (MQ=255)
                                           ttACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATAcc                                                                                               >  5:946227/1‑140 (MQ=255)
                                                gAACCACAGCGAATACTGTAGCCAGCCCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGAc                                                                                          >  3:1002433/1‑140 (MQ=255)
                                                  aCCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGCCGACTCTACATCCTCTGTTGGGGATACCGCGACaa                                                                                        >  3:147419/1‑140 (MQ=255)
                                                  aCCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATATTCATCCCATGCATCCGAAGCCGACTCTACATCCTCTGTTGGGGATACCGCGACaa                                                                                        >  5:644251/1‑140 (MQ=255)
                                                         cGAATACTGAACCCAGCCCCTAATCCCCCGAAACCCCAGCTTTAGTTAACCC‑ACCCTGCCCCCTCAAAACCGCCATCTTCACCCCATGCATCCGACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGc                                                                                 <  1:110596/140‑1 (MQ=255)
                                                           aaTACTGTAGCCAGACCAGAATAGCCATCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACCGCTACTTCATCCCATGCATCCGAAGACGACTCTACATCCAAAGTTGGGGATACCGCGACAACGCGGGCaa                                                                               >  6:640672/1‑140 (MQ=255)
                                                             tACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAAt                                                                             >  4:365492/1‑140 (MQ=255)
                                                                 gTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGCCAACGCGGGCAAATGTAc                                                                         >  6:929996/1‑140 (MQ=255)
                                                                 gTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTAc                                                                         >  1:839459/1‑140 (MQ=255)
                                                                  tAGCCAGACCAGAATAGCCAGCAACACCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGCCTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTAcc                                                                        >  7:805209/1‑140 (MQ=255)
                                                                   aGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCGTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACca                                                                       >  4:277371/1‑140 (MQ=255)
                                                                     ccagaccagaATCCCCACCAACAGCAGCGTTAGTTTCCCC‑AACCTCCCCCCTGAAAAACGCCATCTTCAACCCTTCCATCCCAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACcaca                                                                     <  2:405084/140‑1 (MQ=255)
                                                                     ccagaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACcaca                                                                     >  7:905604/1‑140 (MQ=255)
                                                                     ccagaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACcaca                                                                     >  1:947732/1‑140 (MQ=255)
                                                                     ccagaccagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCCACAACGCGGGCAAATGTACcaca                                                                     >  5:383989/1‑140 (MQ=255)
                                                                         accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCTTCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTg                                                                 >  6:850692/1‑140 (MQ=255)
                                                                         accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACAtttt                                                                 >  2:161374/1‑139 (MQ=255)
                                                                         accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACAtttt                                                                 >  4:71189/1‑139 (MQ=255)
                                                                         accagaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑AACCTCCCCCCTGAAAAACGGCATCTTCATCCCATGAATCCGAAGACGACTCTACATCCTATGTTGGGGATACCGCGACAACGCGGCCAAATGTACCAAATTTg                                                                 >  8:728247/1‑140 (MQ=255)
                                                                            agaATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCg                                                              >  7:483021/1‑140 (MQ=255)
                                                                             taaTACCCAGCACCAGCAGTTTTAGTTAACCC‑ATCCTGCCCCATAAAAACCGGAATATTAATCCCATCCAACCTAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGa                                                             <  2:540518/139‑1 (MQ=255)
                                                                               atccccaccaACCGCAGCGTTAGTTTCCCC‑ATCCGCCCCCCTGAAACCCGGCATCTTCATCCCATGCATCCCCAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGAtt                                                           <  2:253854/136‑1 (MQ=255)
                                                                                 cgcccccAACCGCAGCGTTAGTTTACCC‑AACCTCCCCCCTGAAAAACGGCATCTTCAACCCATGCACCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  4:497488/134‑1 (MQ=255)
                                                                                 cgCCAGAAACCGCCGCGTTAGTTAACCC‑ATCCTCCCCCCTCAAAACCGGCATATTCATCCCAGGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  3:67486/139‑1 (MQ=255)
                                                                                 cgCCACCAACCGCAGCTTTATTTAACCC‑AACCGCCCCCCTTAAAAACGCAATCTTCATCCCATCCAGCCCAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  1:177626/139‑1 (MQ=255)
                                                                                 cccctccAACAGCTGGTTTAGTTAACCC‑AGCCTCCCCCATGAAAAAGGGCATGTTCAACCCATCCATCCGACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  1:738859/134‑1 (MQ=255)
                                                                                 ccccAGCAAATCCAGCTTTTTTTTACCC‑AGCCTCCCCCCACAAAACCGGATTCTACACCCCAGCCATCCGACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  7:57748/138‑1 (MQ=255)
                                                                                 acccaccaccaGCAGGGTCAGTTAACCC‑ATCCTCCCCCGTCAAACCCGCCATCTGCACCCCATGAATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  8:259301/138‑1 (MQ=255)
                                                                                 aGCCACCAACAGCAGCGTTA‑TTTACCCAATCCTGCCCCATTAAAACCGTCATCTTAATCCCATGGCTGCGAAGAAGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGt                                                         <  1:35046/140‑1 (MQ=255)
                                                                                   ccAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTa                                                       >  5:513838/1‑140 (MQ=255)
                                                                                     cccAACAGCACCGTTAGTTAACCC‑ATCCTCCCCCCTCAAAACCGGCATCTTCAACCAATCCATCCCACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                                     <  6:299654/138‑1 (MQ=255)
                                                                                     acaaaCAGCAGTGTTAGTTAACAC‑ATCCTGCCCCCTTAAAAACGGCATCTTCATCCCATGCATCCTACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGAGTGTTACg                                                     <  8:89905/137‑1 (MQ=255)
                                                                                     aGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                                     <  8:222943/140‑1 (MQ=255)
                                                                                     aGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACg                                                     <  4:489919/140‑1 (MQ=255)
                                                                                     aGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTATACATCCTCTGTTGGGGATACCGCGACAACGCGGTCAAATGTACCACATTTGTCGATTGTTACg                                                     >  1:599259/1‑140 (MQ=255)
                                                                                     aGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTAATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGGACCACATTTGTCGCTTGTTAcc                                                     >  5:164329/1‑139 (MQ=255)
                                                                                       cAACAGCTGCTTTAGTTTACCC‑ATCCTCCCCCCTTAAAAACGGCATCTTCATCCCATGCATCCTAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGta                                                   <  2:792406/140‑1 (MQ=255)
                                                                                             cagcGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACTACTCTACATCATCTGTTTGGGATAACGCGACAACGCGGGCAAATTAACCACATTTGTCGATTTTGACGTATACCCa                                             >  7:341113/1‑140 (MQ=255)
                                                                                               gctttAGTTAACCC‑ATCCTGCCCCCTCAAAACCGGCATCTTCATCCCATGCATCCCAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAgg                                           <  4:1002432/137‑1 (MQ=255)
                                                                                               gcGTTTGTTTACCC‑ATCCTCCCCCCTGAAAACCGCCATCTACATCCCATCCAGCCCAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAgg                                           <  2:974721/140‑1 (MQ=255)
                                                                                               gcGTTATTTTACCC‑ATCCTCCCCCCTTAAAACCGGCATCTTCAACCCATCCAGCCGAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAgg                                           <  2:133257/140‑1 (MQ=255)
                                                                                               gcGTTAGATTACCC‑ATCCTCCCCCCTCAAAACCGGCAGCATCAACCCATCCACCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAgg                                           <  5:317273/140‑1 (MQ=255)
                                                                                                 tttAGTTACCCC‑AACCTGCCCCCTGAAACACGGCAGCTTCAGCCCATGCATCCCAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGc                                         <  4:286672/139‑1 (MQ=255)
                                                                                                    attttACCC‑AACATCCCCCCTAAAAACCGGCATTTTCATCACAAGCAACGGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAAAGCGGGCAAATGTACCACTTTTGTCGATTGTTACGTATACCCAGGGCGTg                                      <  3:431886/138‑1 (MQ=255)
                                                                                                    agttacccc‑AACCTGCCCCCTCAAAACCGGCATCATCATCCATTGCACCCCACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGACTGTTACGTATACCCAGGGCGTg                                      <  1:141312/134‑1 (MQ=255)
                                                                                                     gTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGc                                     >  5:270553/1‑140 (MQ=255)
                                                                                                     gTTTACCC‑AACCTGCAACCTGAAAAACGGCATCTTCAACCCATGCATCCGAAGACGACTCTACATCCTAACTAGGGGATACCGCGACAACGCGGGCAAATGAACCACATTAGTCGATAGTAACGTATACCCACGGCGtta                                     >  5:306423/1‑138 (MQ=255)
                                                                                                      tttACCC‑AACCTCCCCCCTCAAAAACGGCATCTACATCCCATGCATCCGAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCa                                    <  7:446499/140‑1 (MQ=255)
                                                                                                       tcaCCC‑CTCCTCCCCCCTCAAAAACGCCAGCTACAACCCTGGCATCCGACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAg                                   <  2:159494/138‑1 (MQ=255)
                                                                                                          ccc‑ATCCTGCCCCCTGAAAAACGGCATATTCATCCCATGCATCCGAAGACGACTCTAAATCCTCTGTTGAGGATACCGATACAACGCGGGCAAATGGACCACATTTGTAGATTCTTAAGTATACCCATGGCGTGCAGaaa                                >  5:57827/1‑139 (MQ=255)
                                                                                                           cc‑ACCCTCCCCCCTCAAAAACGCCATAATCATCCCTTGCAGCCCAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACa                               <  5:803092/140‑1 (MQ=255)
                                                                                                            c‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACAt                              >  3:550282/1‑140 (MQ=255)
                                                                                                                ccTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAAt                           >  6:14314/1‑140 (MQ=255)
                                                                                                                ccTGCCCCCTGAAAAACGGCATATTCATCCCATGCATCCGAAGACGACTCTACATCCTCTCTTGGGGATACCGCGACAAAGCGGGCAAATGTACCACATTTGTCGATTGATACGTATACCCAGGGCGTGAAGAACATAAt                           >  5:866910/1‑140 (MQ=255)
                                                                                                                ccTGCCCCCTGAAAAACGCCATCTTCACCCCAGGAACCCGAACACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAAt                           <  3:389766/140‑1 (MQ=255)
                                                                                                                         tGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCattatt                  >  4:508329/1‑140 (MQ=255)
                                                                                                                         tGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCattatt                  >  4:689144/1‑140 (MQ=255)
                                                                                                                                    atattCATCCCTTGCACCCGACCACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGAtt       <  3:120657/137‑1 (MQ=255)
                                                                                                                                    aTCTTCATCCCATGCAACCTAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGAtt       <  2:103364/140‑1 (MQ=255)
                                                                                                                                     tCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGGTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGCACATAATCTCATTATTAGTTACGGATTg      >  8:13901/1‑140 (MQ=255)
                                                                                                                                         attCCCATGCATCCCACGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGATTGAAtt  <  3:301188/138‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CGCCACATCATTATTGACGCGAGTATAGTCATGTATACCGTTCTTACCGAACCACAGCGAATACTGTAGCCAGACCAGAATAGCCAGCAACAGCAGCGTTAGTTTACCC‑ATCCTGCCCCCTGAAAAACGGCATCTTCATCCCATGCATCCGAAGACGACTCTACATCCTCTGTTGGGGATACCGCGACAACGCGGGCAAATGTACCACATTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGATTGAATT  >  NZ_CP022686/1479481‑1479756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A