Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,252,190 T→G 15.1% K221N (AAA→AAC)  CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,252,1900TG15.1% 172.3 / 6.5 91K221N (AAA→AAC) CIW80_RS16225enoyl‑CoA hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base T (14/61);  new base G (14/2);  total (28/63)
Fisher's exact test for biased strand distribution p-value = 3.10e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAACTTACGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCTTTTTCTACCACTTCATCAATTAAATTCGC  >  NZ_CP022686/3252053‑3252324
                                                                                                                                          |                                                                                                                                      
gATAACTTACGCATACAATTTACTTATTGATTGTGGAAGCTCACTCCGTCTTTTTAGGCTAATTCGAGCGTCGCCTGTTGGCACTCCTGAGTGGCTGACATTAATCCAGCCAGGAACTGGTGGGAGTAATCGGCGT‑Gttt                                                                                                                                      >  3:998655/1‑140 (MQ=255)
     cTTACGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑Gtttttttt                                                                                                                                 >  3:737170/1‑140 (MQ=255)
        ccGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGTCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCa                                                                                                                              <  3:626115/139‑1 (MQ=255)
        aCGCATACAATTTCCTTATTGATTGTGGAAGCTCGCGCCGGCTTTTTCGGCATATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTGCa                                                                                                                              >  8:259718/1‑140 (MQ=255)
        aCGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCa                                                                                                                              <  5:1043731/140‑1 (MQ=255)
        aCGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTttttttgcc                                                                                                                              >  5:843531/1‑137 (MQ=255)
        aCGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGAATGTTGGCAATCCTGAGTGTCGGACATTAATCCAGCCAGTAACTGGTGGTAGTAATCGGCGT‑GTTTTTTTTACa                                                                                                                              >  1:903135/1‑140 (MQ=255)
        aCGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTACGGCACATGCGTGCGTCGCCTGTTGGCAAGCCTGAGTGGCGGAAATTAATCCAGCCAGTAACTGGAGGGAGTAATCGGCGT‑GTGTAttttaag                                                                                                                              >  1:848902/1‑137 (MQ=255)
        aCGCATACAATTTCCTTATTGAGTGTGGAATCTCACGCCGGCTTTTTCGGCATATGCGTGCGTCGCCTGTCGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCa                                                                                                                              <  2:491506/140‑1 (MQ=255)
        aCGCATACAATTTCCTTATCGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGTGGTTTTTTTTcc                                                                                                                               >  1:21052/1‑140 (MQ=255)
           cATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTTTTTTACAgcg                                                                                                                           >  5:932102/1‑140 (MQ=255)
           cATACAATTTCCTTATAGATTGTCGAAGCTCACGCCGGCTTTATCGGCAAAAGCGTGCGTCGCCAGATCGAAATCCTGAGAGGCGGACATTAATACAGCCACTAACAGGTCGGAGTAATCGGCGT‑GTTATttttaaaccc                                                                                                                           >  7:50498/1‑134 (MQ=255)
             tACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTTTTTTCCAGAGcc                                                                                                                         >  7:479547/1‑140 (MQ=255)
                    tCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCa                                                                                                                  <  1:680425/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  4:640399/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  3:196543/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  2:330320/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  3:713376/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  3:1087945/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  3:1085705/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  3:949417/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  4:1044234/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  4:1046502/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  5:1002181/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  6:379982/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  6:460472/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  8:664570/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAAGCGGCGG‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  8:9745/140‑1 (MQ=255)
                     ccTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTGGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAg                                                                                                                 <  1:557816/140‑1 (MQ=255)
                        tATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTTTTTTGCAGCGCCAGCATCAGATg                                                                                                              >  1:73775/1‑140 (MQ=255)
                          ttgattgTGGCAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  1:330941/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  2:716536/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  1:869278/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  6:712966/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  4:505259/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  2:596957/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  2:196368/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  1:966252/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  3:781873/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  3:783116/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  7:589175/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTAGCATGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  5:419220/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGCCGGCTATTTCGGCACATGCGTGCGTCGCCTGGTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGGGTGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  7:125012/140‑1 (MQ=255)
                          ttgattgTGGAAGCTCACGACGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCt                                                                                                            <  2:750404/140‑1 (MQ=255)
                                gtgGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAatat                                                                                                      >  2:15505/1‑140 (MQ=255)
                                gtgGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTACCGCCCCCGAAACAGGTGCTTAaaat                                                                                                      >  5:255623/1‑140 (MQ=255)
                                gtgGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGGTTTTTTGCAGCCCCAGAAAAAGTTTCTTAataa                                                                                                      >  5:260804/1‑139 (MQ=255)
                                     aGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATgtgcc                                                                                                 <  8:47152/140‑1 (MQ=255)
                                       cTCACGCCGGCTTAGTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTATCCAGCGCCAGAATCAGTTGCTTAATATgtgccag                                                                                               <  4:784788/140‑1 (MQ=255)
                                                 cTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTTTTTTACAGCGCCAGAATCAGTTTCTTAAATTGTTCCAGGGCCACCggg                                                                                     >  7:83606/1‑139 (MQ=255)
                                                 cTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTTTTTTCCAGGGCCAGAATCATGTGCTTAATATTTGCCCGTGCcccccgg                                                                                     >  2:122084/1‑137 (MQ=255)
                                                 cTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTACAGCGCCAGAATCAGTTGGTTAATATTTGGCAGTGCCCCCGGa                                                                                     >  4:1075476/1‑140 (MQ=255)
                                                  tttttCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGAc                                                                                    <  7:626907/140‑1 (MQ=255)
                                                     ttCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACcac                                                                                 <  5:714140/140‑1 (MQ=255)
                                                               gcgtgcgtCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAg                                                                       <  6:578517/140‑1 (MQ=255)
                                                               gcgtgcgtCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAg                                                                       <  2:849532/140‑1 (MQ=255)
                                                               gcgtgcgtCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAg                                                                       <  8:39992/140‑1 (MQ=255)
                                                               gcgtgcgtCGCCTGTTGGCAATCCTGAGTGGAGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGG‑GGTTATTTTCCAGCTCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAg                                                                       <  4:575504/140‑1 (MQ=255)
                                                               gcgtgcgtCGCCTGGTTGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAg                                                                       <  3:798524/140‑1 (MQ=255)
                                                                  tgcgtCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTGAAGCGCCAGAATCAGTTTGTTAATATGTGGCAGTGCCCCCGGGCCCCAGATTTACAGCTg                                                                    >  1:676737/1‑140 (MQ=255)
                                                                      tCGCCTGTTGGCAATCCTGAGTGGCTGACATTAATCCAGCCAGTAACTGCTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCt                                                                <  4:986335/140‑1 (MQ=255)
                                                                      gcGCCTGTTGGCAATCCTGAGGGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCt                                                                <  1:30711/139‑1 (MQ=255)
                                                                        gCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTaa                                                              <  5:69091/140‑1 (MQ=255)
                                                                              tGGCAATCCTGCGTGGCTGACATTAAACCAGCCAGTAACTGGTGGGAGTAATCGTCGT‑GGTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGGGCCAGTGCCACCGGACCACATTTATACAGCTGCGCTAACTGGtt                                                        <  1:409720/140‑1 (MQ=255)
                                                                              tGGCAATCCGGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTAGCTTAATATGAGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGtt                                                        <  7:325160/140‑1 (MQ=255)
                                                                                gCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTTGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCg                                                      <  3:204911/140‑1 (MQ=255)
                                                                                    tCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCAt                                                  >  4:850055/1‑140 (MQ=255)
                                                                                    tCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTGTATTTTGCACCGCCAGAATCAATTTGGTAAAAAGTGCCAGTGCCCCCGGACCCCAATATTTCAACTGCGCGAACATGTTCGACaa                                                  >  1:20917/1‑139 (MQ=255)
                                                                                    tCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTAAAGGCGCAGAAAACGTTGGTTTATATGTGCCCGGGCCACCGGACCACATTTTTCACTGTGCGCTACATGTTTAGCCAt                                                  >  2:918945/1‑140 (MQ=255)
                                                                                     ccTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATc                                                 <  1:133158/140‑1 (MQ=255)
                                                                                     ccTGAGTGGCGGACATTAATCCAGACAGTAACTGGTGGGAGTAATCGGCGG‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATc                                                 <  2:709707/140‑1 (MQ=255)
                                                                                      cTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATcc                                                <  1:15505/140‑1 (MQ=255)
                                                                                      cTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATcc                                                <  3:286676/140‑1 (MQ=255)
                                                                                      cTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGGTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATcc                                                <  7:197004/140‑1 (MQ=255)
                                                                                         aGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTACAGCGCCAGAATCAGTTGCTTAATATTTGCCCGTGCCACCGGACCACATTTTTACAGCTGCGCTAACTGGTTAGCCATCCAGc                                             >  6:308470/1‑140 (MQ=255)
                                                                                         aGTGGCGGACATTAATCCAGCCAGGAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTACAGCGCCAGAATCAGTTGCGTAATATGTGCCCGGGCCCCCGGCCCCCAGTTTTACCGATGGGGGAACTGGGTCGCCCTACAGc                                             >  7:806413/1‑140 (MQ=255)
                                                                                           tGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCtt                                           <  7:488537/140‑1 (MQ=255)
                                                                                                gACATTTATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGGTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCAc                                      <  3:428579/140‑1 (MQ=255)
                                                                                                gACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCAc                                      <  5:179291/140‑1 (MQ=255)
                                                                                                gACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GGTTATTTTCCAGCGCCAGAATCAGTTGCGTAATATGTGCCAGTGCCACCGGGCCACATTTTTCCAGCATCGCGTACATGTTGGCCAACCAGCTTTacac                                      >  4:694379/1‑140 (MQ=255)
                                                                                                     tAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGtt                                 <  1:420807/140‑1 (MQ=255)
                                                                                                       aTCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCt                               <  5:789038/140‑1 (MQ=255)
                                                                                                       aTCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCt                               <  8:218371/140‑1 (MQ=255)
                                                                                                             ccagTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCCGCGCCAGAATCAGTTGCTTAATATTTGCCAGTGCCCCCGGACCCCATTTTTCCCGGTGCGCTAACTGGTTGGGCATCCAGATGTCACCCTGTTGtttttat                         >  6:855112/1‑138 (MQ=255)
                                                                                                             ccagTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCTTTTTCt                         <  2:18002/140‑1 (MQ=255)
                                                                                                             ccagTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCTTTTTCt                         <  1:766738/140‑1 (MQ=255)
                                                                                                             ccagTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTACAGCGCCAGAATAAGTTTCTTAATATGTGCCAGTGGCACCGGACCACATTTTTACAGCAGCGCTAACTGGTTCGCCCTACAGCATTGACCCTGTTCtttttgt                         >  7:944339/1‑138 (MQ=255)
                                                                                                             ccagTAACTGGGGGGAGTAATCGGCGT‑GTTTTTTTTACAACGCCAGAATAAGTTGCTTAATATTTGCCAGTGCCCCCGGCCCACATTTTTACAGCTGCGCAAAAAGGGTCGCCATACAGCATTCACCCAGTTCtttttag                         >  2:1054980/1‑138 (MQ=255)
                                                                                                                       gtggGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTAGCCATCCAGCTTTCCACCTGTTCTTTTTCTACCACTtcat               <  3:1066887/140‑1 (MQ=255)
                                                                                                                       gtggGAGTAATCGGCGT‑GGTTATTTTCCCGCGCCAGAATCAGTTGCTTAATATGTGCCAGGGCCCCCGGACCCCATTTTTCCAGCGGGGCAAACTGGTTCGCCCTACAGCTTTCCCCCTGTTCTTTTTATAGCACtggat               >  8:86605/1‑136 (MQ=255)
                                                                                                                            aGTAATCGGCGT‑GGTTTTTTTCCAGCGCCAGAATCAGTTGCGTTATATTTGCCAGTGCCCCCGGCCCACATTTTTACAGCTGCGCTAACTGGTTCGCCATCCAGCTTTGCACCTGGTCTTTTTATACCCCTAGAGCAAtt          >  4:795107/1‑140 (MQ=255)
                                                                                                                                 tCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTTCCAGTGCCACCGGACCACATTTTTAAAGCTGCGCTAAATGGTTCGCCAAACCGCTTTCCACCTGTTATTTTTCTACAACATCATCAATTAAAtt     >  8:243051/1‑140 (MQ=255)
                                                                                                                                    gCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCTTTTTCTACCACTTCATCAATTAAATTcgc  <  3:850055/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
GATAACTTACGCATACAATTTCCTTATTGATTGTGGAAGCTCACGCCGGCTTTTTCGGCAAATGCGTGCGTCGCCTGTTGGCAATCCTGAGTGGCGGACATTAATCCAGCCAGTAACTGGTGGGAGTAATCGGCGT‑GTTTATTTTCCAGCGCCAGAATCAGTTGCTTAATATGTGCCAGTGCCACCGGACCACATTTTTCCAGCTGCGCTAACTGGTTCGCCATCCAGCTTTCCACCTGTTCTTTTTCTACCACTTCATCAATTAAATTCGC  >  NZ_CP022686/3252053‑3252324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A