Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,371,081 C→A 18.2% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,371,0810CA18.2% 223.8 / 11.1 127P32Q (CCA→CAA) bamDouter membrane protein assembly factor BamD
Reads supporting (aligned to +/- strand):  ref base C (73/27);  new base A (2/25);  total (75/52)
Fisher's exact test for biased strand distribution p-value = 4.60e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GAGAATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGC  >  NZ_CP022686/1370946‑1371219
                                                                                                                                       |                                                                                                                                            
gagaATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAAt                                                                                                                                          >  1:302536/1‑140 (MQ=255)
gagaATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAAt                                                                                                                                          >  3:84464/1‑140 (MQ=255)
gagaATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCGGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAAt                                                                                                                                          >  6:144502/1‑140 (MQ=255)
   aaTCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGaa                                                                                                                                       >  3:470430/1‑140 (MQ=255)
     tctcCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAAt                                                                                                                                     >  1:83054/1‑140 (MQ=255)
     tctcCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAAt                                                                                                                                     >  2:26699/1‑140 (MQ=255)
     tctcCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATATGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAAt                                                                                                                                     >  5:294002/1‑140 (MQ=255)
     tctcCCGTATTACATATTGAGGAAAGTCAAAACTTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCAGCCAAATGAAAt                                                                                                                                     >  8:652545/1‑140 (MQ=255)
      ctcCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGCCGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAAtt                                                                                                                                    >  7:732686/1‑140 (MQ=255)
        cccGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑a                                                                                                                                >  1:971910/1‑140 (MQ=255)
         ccGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCGGATAATCCGCCAAATGAAATT‑T‑Ac                                                                                                                               >  7:590559/1‑140 (MQ=255)
           gTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCTGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑Acgc                                                                                                                             >  1:169561/1‑140 (MQ=255)
           gTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑Acgc                                                                                                                             >  1:948777/1‑140 (MQ=255)
             tataaaTTTTGACTAAAGTAAAAACGTCATGACGCGCATTAAATATGTGGGGGCCGCCGCCACAGTAAGCGTTTTTTGGGGGGGTTGCGGGGTGTAAAAGGAATAAGAACCTGTTAATCCGCAAAATCAATTT‑C‑ACGCGa                                                                                                                           <  6:749304/135‑1 (MQ=255)
                 cATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGc                                                                                                                       >  4:880000/1‑140 (MQ=255)
                 cATTTAGAGTAACGTCAAACCGACATTAGGCCCATTAAATATGTGGGGGAAGCCGCCAAAATAAGGCTGTTTCTGGCGGGTTGCTCGGGGTCAAAGAAACAAGTACCTGATAATCCGCAAAATTAAATT‑T‑CCGCGACTGc                                                                                                                       <  2:874910/140‑1 (MQ=255)
                   ttttGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCCCACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACATGATAATCCGCCAAATGCAATT‑T‑ACGCGACTGcac                                                                                                                     >  2:33819/1‑140 (MQ=255)
                      tgatgaAAGTCAAAACGTAATGACGCGCATTAAATATGTGGTGGCAGCCGCAACACTAAGCCTGTTTTTGGGGGGTTGCGGGGGGTAAAAGTAATAATTACCTGATAATCCGCCAAATTAAATT‑T‑ACGCGACTGCacaac                                                                                                                  <  6:125723/140‑1 (MQ=255)
                      tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAATTT‑T‑ACGCGACTGCacaac                                                                                                                  >  1:409545/1‑140 (MQ=255)
                      tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCacaac                                                                                                                  >  8:468961/1‑140 (MQ=255)
                      tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCacaac                                                                                                                  >  3:258685/1‑140 (MQ=255)
                      tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCacaac                                                                                                                  >  2:965364/1‑140 (MQ=255)
                        aGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCAcaacaa                                                                                                                >  6:87709/1‑140 (MQ=255)
                        aGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTG‑TTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACaaa                                                                                                               >  7:774514/1‑140 (MQ=255)
                             aGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTTCCTGATAATCCGCCAAATGAAATT‑T‑ACGCGCCTGCACAACAAAAGCt                                                                                                           >  1:875138/1‑140 (MQ=255)
                             aGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTGGCTCGGGGTCAAAGGCAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAACAGcg                                                                                                           >  4:857524/1‑139 (MQ=255)
                              ttaaaaaCGTCATGACGCGCATTAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGGGGGTTGCTCGGGGTCAACGGAAGAAGTACCTGATAATCCGCAAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTg                                                                                                          <  2:959588/137‑1 (MQ=255)
                                 aaaaCGTCATGCCGCGCATTAAATATCTGGTGGCAGCCGCCACACTAAGCGTGTTTTTGGCGGGTTGCTCGGGGTAAAAGGAAGAAGTACCTTATAATCCGCAAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAg                                                                                                       <  4:188363/140‑1 (MQ=255)
                                       tCATGACGCGCATTAAATATCTGGTGGCAGCCGCAACACTAAGCCGGTTTTTGGGGGGTTGCTCGGGGACAAAGGAAGAAGTACCTGATAAACCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGt                                                                                                 <  7:313058/140‑1 (MQ=255)
                                         aTGTCGCGCATGAAATATAGGGTGGCAGCCGACACAATAAGCATTTTGTTGGCGGGTTGCTGGGGGTCAAAGCCACATGAACCTTATAATCCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTaa                                                                                               <  5:836830/140‑1 (MQ=255)
                                         aTGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAACAGCTGCAGGACGGTaa                                                                                               >  3:25658/1‑140 (MQ=255)
                                               cgcATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑TCGCGACTGCACAACAACAGCTGCAGGACGGTAACTGgag                                                                                         >  2:266424/1‑140 (MQ=255)
                                               cgcATGAAATATCTGGTCGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTAGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGAGTGCACCACAAAAGCTGCAGGACGCTAACTGgag                                                                                         >  1:261868/1‑140 (MQ=255)
                                               cccATCAAATATCTGGTGGCCGCCGCAAAACTACGCCGGTTTTTGGGGGGGTGGTCGGTTTCATCGTAACAATTACAGGATAATCCGCAAAATTAATTT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGgag                                                                                         <  4:913/138‑1 (MQ=255)
                                                gcATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGCCGGTAACTGgaga                                                                                        >  6:694802/1‑140 (MQ=255)
                                                gcATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCGGGGGGTCAAAGGAAGAAGTACCTGATAATCCGCAAAATGAAATT‑T‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGgaga                                                                                        <  1:747204/140‑1 (MQ=255)
                                                gcATGAAATATCTGGTGGCAGCCGCCACAATAAGCCTGTTTTTGGCGGGTTGCTCGGGGTAAAAGCAATAAGTACCTGATAAACCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGgaga                                                                                        <  1:546812/140‑1 (MQ=255)
                                                caattaaaTATTTGGGGGCACCGCCCACACTAAGCCTGTTTTTGGGGGGTTGCGCGGGGTCAACGGATCACTTACCTTATAATCCGCAAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGgaga                                                                                        <  2:17273/135‑1 (MQ=255)
                                                 cATGAAATTTGTGGTGCCCGCCGCCAAAATATGCTTGTTTTTGGCGGTTTGCTGGGGTTCAACGCAATAATAACGTGATAATCCGCAAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGAc                                                                                       <  2:483589/140‑1 (MQ=255)
                                                 cATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGAc                                                                                       >  6:616331/1‑140 (MQ=255)
                                                 cATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGAc                                                                                       >  4:72135/1‑140 (MQ=255)
                                                 cATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGAc                                                                                       >  1:73984/1‑140 (MQ=255)
                                                  tttaattatGTGGTGCCAGCCGCCACAATAAGCGTGTTTTTGGGGGGTTGGTGGGGGTCAATGTAATAATAACCTGATAATCCGCAAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACa                                                                                      <  5:839105/134‑1 (MQ=255)
                                                  attaaaTATCGGGTGGCAGCCGCCACAATACGCCTGTTTTTGGGGGGTGGCGGGGGGTAAAAGGAAGAAGTACCTTATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACa                                                                                      <  4:281078/137‑1 (MQ=255)
                                                        tctcgggtgtCAGCCGCAACACTAAGAGTTTTTTTGTGGTTTTGATGGGTTTAAATGTAATAAGAACCTTATAATCC‑CAAAATTTAATT‑TAACGAGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAAt                                                                                <  7:468961/135‑1 (MQ=255)
                                                            tggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTGGGGGTCAAAGGAAGAAGTACCTGATATTCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCTATAACg                                                                            >  5:212431/1‑140 (MQ=255)
                                                            tggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAAGAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACg                                                                            >  5:212436/1‑140 (MQ=255)
                                                            tggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACg                                                                            >  5:258634/1‑140 (MQ=255)
                                                            gggtggCTGCCTCCACTATACGCCTGTTTTTGGCGGGTTGCGGGGGTTAAACGTATTAAGACCGTCATATACCGCCAATTTAATTT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACg                                                                            <  4:293338/139‑1 (MQ=255)
                                                            gggggtcAGCCGCAACAATATGCCTGTTGTTGGCTGGTTGCTAGGGGTCAACGGAACACGTACCTTATAATACGCAAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACg                                                                            <  6:619102/134‑1 (MQ=255)
                                                              gtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAACAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCa                                                                          >  7:592539/1‑140 (MQ=255)
                                                               tggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCaa                                                                         >  4:628058/1‑140 (MQ=255)
                                                                 gCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACt                                                                       >  2:324039/1‑140 (MQ=255)
                                                                 gCAGCCGCCACACTAAGCCTGTTTTAGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACt                                                                       >  8:769426/1‑140 (MQ=255)
                                                                   cgccgccACACTATGCGTGTTTTTGGGGGGTTGCTCGGGTCCAAAGTATTAAGTACCTTATAAACGGCCAATTTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTgg                                                                     <  5:551075/139‑1 (MQ=255)
                                                                   cgccgccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTAAAAGTAAGAAGTACCTGATAATCCGCCAAATGAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTgg                                                                     <  4:84464/139‑1 (MQ=255)
                                                                   cgccgcaACAATAAGCCTGTTTTTGGCGGGTTGCTCGGGTAAAACGGACTAATTACATTATAATCCGACAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGCCAGGCAATCACGCAACTgg                                                                     <  8:538489/139‑1 (MQ=255)
                                                                    gccgccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGATGAAGTAACTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCCCGACAAAAGCTGCAGGACGGTAACTGGCGACAGGCAATAACGCTACTGGa                                                                    >  8:724340/1‑140 (MQ=255)
                                                                        ccACACTACGCCTTTTTTTGGCGGGGTGCGCGGGGTCAACGTAATACGAACCTTATAACCCCCCAAATTAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCg                                                                <  2:302536/140‑1 (MQ=255)
                                                                         cacaCTAAGCCTGTTTTTGGCGGGTTGCGCGGGGTCAAAGGAAGAATTACCTGAAAATCCGCCAAATGAAATT‑A‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGt                                                               <  6:554622/140‑1 (MQ=255)
                                                                         cacaCTAAGCCTGTTTTTGGCGGGTGGCGCGGGGTCAAAGGAACACGTACCTGATAATCCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGt                                                               <  4:673097/140‑1 (MQ=255)
                                                                         aacacTAAGCTTGTTTTGGGGGGGTTGCGGGGGGTAAAAGCAATAAGTACCTGAAAATCCGCAAATTTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGt                                                               <  4:289062/139‑1 (MQ=255)
                                                                         aacacTAAGCCTTTTTTGGGCGGGTGGCTCGGGGTCAAAGGAACAAGTACCTTATAATCCGCAAATTTAAATT‑A‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGt                                                               <  5:122004/139‑1 (MQ=255)
                                                                         aacacTAAGCCTGTTTTGGGCGGGGTTCTGGGGGTCAACGGAAGAATTACCTTATAATCCGCAAAATTAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGt                                                               <  2:923397/139‑1 (MQ=255)
                                                                             cTAACCCTGTTTTTGGCGGGTGGCGCGGGCTCAACTGAACAAGACCCTTATAATCCGCCAAATGAAATT‑A‑ACGCGACTGCACACCAAAATCAGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGa                                                           <  4:865635/140‑1 (MQ=255)
                                                                              aaagcgggTTTTGGGGGGGTGGCGCGGGTTAAAAGTAATAATAACCTCAAAACCCGCAAAATTAAATT‑T‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGAt                                                          <  1:267323/133‑1 (MQ=255)
                                                                                aGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATaa                                                        >  1:633487/1‑140 (MQ=255)
                                                                                aGCCTGTTTTTGGCGGGTTGCTCGGGGGCAAAGGAAGAAGTACCTGAGAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAACGCTGCCGGACGGTAACTGGAGACAGGCAATAACGCACCTGGCAGCGGTAGATaa                                                        >  5:231190/1‑140 (MQ=255)
                                                                                 gcgggTTTGTGGGGGGTTGCGCGGGGCCAAAGGACCAAGTACCTGATATTCCCCAAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAAt                                                       <  8:159920/136‑1 (MQ=255)
                                                                                  ccTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATc                                                      >  1:54891/1‑140 (MQ=255)
                                                                                      tttttGGCGGTTTGCTCGGGTTCAAAGGATGAAGAAACTTATAAAACGCAAAATGAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTa                                                  <  8:445537/140‑1 (MQ=255)
                                                                                      tttttGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAAACCGCAAAATGAAATA‑A‑ACTCGAAAGCACAACAACAGCTGAAGGAAGGTAACTGGAGACAGGCAATAACGAAACTGTAAGAGTTAGATAATctctg                                                  >  5:87143/1‑139 (MQ=255)
                                                                                      ttgttGGCGGGTGGCTGGGGGGCAAAGGAAGAAGTACCTGATACTCCGACAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTa                                                  <  8:449914/137‑1 (MQ=255)
                                                                                          tGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCg                                              >  5:862965/1‑140 (MQ=255)
                                                                                                       ggggTCAAAGTAATAATTACCTGAAAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTAtt                                 <  2:100766/140‑1 (MQ=255)
                                                                                                          gTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgc                              >  1:100766/1‑140 (MQ=255)
                                                                                                             aaaGGAAGATGTACCTGATATTCCGCAAAATTAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagc                           <  4:305919/140‑1 (MQ=255)
                                                                                                             aaaGGAAGAAGTACCTTATAATCCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagc                           <  4:946600/140‑1 (MQ=255)
                                                                                                              aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                          >  7:61008/1‑140 (MQ=255)
                                                                                                              aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                          >  3:132092/1‑140 (MQ=255)
                                                                                                              aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                          >  3:572147/1‑140 (MQ=255)
                                                                                                                 caacaagAACCTGATAAACCGCAAAATTAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAAGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgctgcacct                       <  1:26699/136‑4 (MQ=255)
                                                                                                                  aataagTACCTTAAAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTg                      <  6:295622/137‑1 (MQ=255)
                                                                                                                   agaagTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACTGTAACTGTAGACAGTCAATAACGCAACTGGAAGCGTTAGATACTCGCTAGACGTTTGGTCCGTATTCGAAGCAGGTGc                     >  3:93065/1‑140 (MQ=255)
                                                                                                                   agaagTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                     >  1:440647/1‑140 (MQ=255)
                                                                                                                      agTACCTTAAAATCCGCCAAATTAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                  <  3:82237/140‑1 (MQ=255)
                                                                                                                        aaCCTGATAATCCGCCAAATTAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTg                <  1:536041/139‑1 (MQ=255)
                                                                                                                          ccTGATAATCCGCCAAATGGAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGa              >  3:57297/1‑140 (MQ=255)
                                                                                                                           cttaTAATCCGCAAATTGAATTT‑T‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt             <  6:861567/137‑1 (MQ=255)
                                                                                                                           cttaTAATCC‑CCAAATTTAATTAT‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt             <  6:788217/137‑1 (MQ=255)
                                                                                                                           cTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGTACTGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTAGCAGCAGTTGCAGATGGaa             >  3:406675/1‑139 (MQ=255)
                                                                                                                           cTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt             >  7:469414/1‑140 (MQ=255)
                                                                                                                           cTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt             <  3:880000/140‑1 (MQ=255)
                                                                                                                           cTGAAAACCCGCCAAATAAAATT‑A‑ACGCGACTGCACAACAAAAGCTGCAGGACTGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTATGGTCCGTATTCGCAGCAGGTGCAGCTGGAt             <  7:677398/140‑1 (MQ=255)
                                                                                                                           atcaaaaTCCGCCAAATTAAATT‑T‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt             <  1:165000/135‑1 (MQ=255)
                                                                                                                            tGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtc            >  1:396323/1‑140 (MQ=255)
                                                                                                                                 aTCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATc       <  4:515218/140‑1 (MQ=255)
                                                                                                                                  tCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCt      >  4:615035/1‑140 (MQ=255)
                                                                                                                                  tCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCt      >  5:420725/1‑140 (MQ=255)
                                                                                                                                    cGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCAGTATTCGCAGCAGGTGCAGCTGGATCTCATCTAc    >  1:130032/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                            
GAGAATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATT‑T‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGC  >  NZ_CP022686/1370946‑1371219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A