Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,371,081 C→A 18.9% P32Q (CCA→CAA)  bamD → outer membrane protein assembly factor BamD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,371,0810CA18.9% 333.9 / 16.2 171P32Q (CCA→CAA) bamDouter membrane protein assembly factor BamD
Reads supporting (aligned to +/- strand):  ref base C (101/32);  new base A (2/36);  total (103/68)
Fisher's exact test for biased strand distribution p-value = 8.48e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

AGAGAATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCC  >  NZ_CP022686/1370945‑1371220
                                                                                                                                        |                                                                                                                                            
agagAATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCaaa                                                                                                                                           >  7:737932/1‑140 (MQ=255)
  agaATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCaaa                                                                                                                                           <  2:860142/138‑1 (MQ=255)
    aaTCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGCCGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATg                                                                                                                                         <  1:321689/138‑1 (MQ=255)
    aaTCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATg                                                                                                                                         >  1:944330/1‑138 (MQ=255)
       ctcCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAAtt                                                                                                                                    >  5:510522/1‑140 (MQ=255)
         cccGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCTCCAAATGAAATTT‑a                                                                                                                                 >  7:23900/1‑140 (MQ=255)
         cccGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGATTTTGGCGGGTAGCTCGGGGTCAAAGGATGAAGTACCTGATAATCCGCCAAATGAAATTT‑a                                                                                                                                 >  8:1070619/1‑140 (MQ=255)
            gTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑Ac                                                                                                                                >  2:114548/1‑138 (MQ=255)
                  cATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGc                                                                                                                        >  3:84267/1‑140 (MQ=255)
                       tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCacaac                                                                                                                   >  3:885886/1‑140 (MQ=255)
                       tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCacaac                                                                                                                   >  5:21274/1‑140 (MQ=255)
                       tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCacaac                                                                                                                   >  3:1033140/1‑140 (MQ=255)
                       tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCacaac                                                                                                                   >  5:661929/1‑140 (MQ=255)
                       tGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCAGATAATCCGCCAAATGAAATTT‑ACGCGACTGCacaac                                                                                                                   >  8:431794/1‑140 (MQ=255)
                         aGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCacaac                                                                                                                   >  2:627656/1‑138 (MQ=255)
                         aGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCAcaacaa                                                                                                                 >  8:782471/1‑140 (MQ=255)
                          gtaaaGTAAAAACGTAATGACGCTCATTAAATATCTGGTGGCCGCCGCCAAAATAAGCCTGTTTTTGGCGGGTTGCTCGGGGTAAAAGGAAGAAGTACCTGATAATCCGCAAAATGAATTTT‑ACGCGACTGCACAACaaa                                                                                                                <  6:208526/138‑1 (MQ=255)
                                taaaaaCGTCATGCCGCGCATGAAATATCTGGTGGCAGCCGCCACACTATGACTGTTTTTGGCGGTTTGCTCGGGGTCAAAGGAAGAAGTACCTTATAATCCCCCAAATGAAATTA‑ACGCGACTGCACAACAAAAGCTGc                                                                                                          <  4:863842/138‑1 (MQ=255)
                                tCAAAACGACATGAGGAGAATTAAATTTCTGGGGGACGCAGACACACTAAGCCTGTTTTTGGGGGGCTGTGCGGGGGCAAAGGACCAAGTACCTCATAACCCGCCAAATTAAATTA‑CCGCGACTGCACAACAAAAGCt                                                                                                            <  2:249671/138‑1 (MQ=255)
                                aaaaaaCGTCAAGCCGCGCATCAAATCTCTGGGGCCCCCCGCCACACAACGCCGTTTTTTGGCGGGGGGCGCGGGGTCAAAGGAATAATTACCTGATAATCCGCAAAATTAAATTA‑CCGCGACTGCACAACAAAAGCTGc                                                                                                          <  6:96470/138‑1 (MQ=255)
                                     ccGTCATGACCCGAATTAAATTTCTGGCGGCAGCCGCCACAGTTTGCGTTTTTTTGGGGGGTGGCTGGGGCTCAAAGTAATAAGTACCTTAAAATCCGCCAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGAc                                                                                                     <  6:1094757/139‑1 (MQ=255)
                                      ggtaaTGCCGCGCATGAAATATCTGGTGGCCGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCGCGGGGTCAACGTAAGAAGTACCTGATAATCCGCCAATTTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGa                                                                                                      <  2:944330/134‑1 (MQ=255)
                                         cATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTa                                                                                                 >  3:944634/1‑140 (MQ=255)
                                          aTGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTaa                                                                                                >  7:185382/1‑140 (MQ=255)
                                          aTGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGACAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAAGTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTaa                                                                                                >  7:343362/1‑140 (MQ=255)
                                                cgcATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGgag                                                                                          >  7:174796/1‑140 (MQ=255)
                                                  cATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGATATTT‑ACTCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGAc                                                                                        >  4:61684/1‑140 (MQ=255)
                                                   tttaaaTATCTGGTGGCAGCCGCCACAATATGCCTGTTTTTGGGGGTTTGCTGGGGTTCAAAGTAATAAGTACCTTATAATCCGCAAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACa                                                                                       <  7:458489/137‑1 (MQ=255)
                                                   attaaaTATCGGGTGGACGCCGCCACACTACGCCGGTTTTGGGCGGGTGGCTGGGGGCAAAAGGAATATTAACCTAATAA‑CCCCCAAATTTAATTTCACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGgaga                                                                                         <  1:1097905/135‑1 (MQ=255)
                                                    tGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGAc                                                                                        >  1:153263/1‑138 (MQ=255)
                                                      aaaTTTCTGGTGGCAGCCTCCACACTACGCCTTTTTTGGGCGGTTTGCTGGGGGTAAAAGTAACATTTACCTTATAAACCGAAAATTTAATTTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGc                                                                                    <  3:1206447/140‑1 (MQ=255)
                                                        atatCTGGGGGCCGCCGCCACAATATGCCTTTTTTTGGCGGGTTGGGGGGGTAAAAAGTAATAATACCCTTATAAACCCCAAATTTAAATTA‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCaa                                                                                  <  3:1009837/140‑1 (MQ=255)
                                                            ttggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGGTCGGTGTAAATGTAAGAAGTACCTTTTAATCCGCAAAATGAAATTT‑ACGCGAATGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAAc                                                                              <  8:579098/139‑1 (MQ=255)
                                                             tggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCTACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACg                                                                             >  4:445825/1‑140 (MQ=255)
                                                             tggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACg                                                                             >  5:716793/1‑140 (MQ=255)
                                                                 ggaaGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGGACCTGATAATCCGACAAATGAAATTT‑ACGCGAATGCAAAACAAAAGCTGCAGGACGTTAACTGGATACAGTCAATAACGCAAc                                                                         >  3:722385/4‑140 (MQ=255)
                                                                 ggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCa                                                                           >  1:746183/1‑138 (MQ=255)
                                                                   cAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCGCGGGGTCAAAGTAAGAATTACCTTATAATCCGCAAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTg                                                                       <  6:689478/140‑1 (MQ=255)
                                                                    cgctgccACACTATGCGTGTTTTGGGGGGGTTGCTCGGGCACAAAGTATCACGTACTTGATAATCCCCAAAATTAAATTA‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTgg                                                                      <  5:155553/136‑1 (MQ=255)
                                                                    cgccgccACACTACGCCTGTTTTTGGCGGGTGGCGCGGGGGCAAAGGAAGACGAACCTGATAATCCGCAAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACt                                                                        <  2:332570/137‑1 (MQ=255)
                                                                    cgccgccACACTACGAGTGTTTTTGGGGGTTTGCGGGGGGTCAAAGCAAGAAGTACCTTATAATCCGCAAATTGAAATTT‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTgg                                                                      <  3:1057560/139‑1 (MQ=255)
                                                                    cgccgccACACTAAGCGTGTTTTTGGCGGGTGGGGGGGGCTAAATGTAATAATTACCTTATAATCCGCAAAATTAAATTT‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTgg                                                                      <  5:130255/139‑1 (MQ=255)
                                                                    cgccgccACACTAAGCCTGTTTTTGGCGGGTGTCTCGGGGTCAAAGGAACAAGTACCTGATAATCCGCCAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACt                                                                        <  2:698500/137‑1 (MQ=255)
                                                                    ccccgccAAACTACGCCTGTTGTTGGCGGGTGGCGGGGGGCAAAAGCAACAAGCCCCTTAAAATCCCCAAAATTAATTTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTgg                                                                      <  7:431390/138‑1 (MQ=255)
                                                                     gccgccACACTAAGCCTTTTTTTGTCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGAAAATCCCCCAAATGAAATTT‑ACCCGACTGCACAAAAAAAGCTGCAGTACGGTAACTGGAGACAGGCAATAACGCAACAGGa                                                                     >  5:919082/1‑140 (MQ=255)
                                                                     gccgccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGa                                                                     >  6:952568/1‑140 (MQ=255)
                                                                         ccaaaatAAGCCTGTTTTGGGCGGGGTGCTCGGGGACAATGTAATAAGACCATTATAACCCGCAAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAg                                                                   <  1:981622/132‑1 (MQ=255)
                                                                               tAAGCCTGTTTTTGGCGGGTTGCGCGGGGTCAAAGGAATAAGTCCCTGATAATCCCCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAg                                                             <  2:427103/138‑1 (MQ=255)
                                                                               aaaGCCTTTTTTTGGCGGGTGGCTCGGGGTCAAAGGACTAATTACCTGATAATCCCCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCATCTGGAAGCGTTAGAt                                                           <  4:13633/139‑1 (MQ=255)
                                                                                 aGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATaa                                                         >  3:294266/1‑140 (MQ=255)
                                                                                 aGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATaa                                                         >  3:260011/1‑140 (MQ=255)
                                                                                 aGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATaa                                                         >  5:1164118/1‑140 (MQ=255)
                                                                                 aGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATaa                                                         >  3:556533/1‑140 (MQ=255)
                                                                                   ccTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATaa                                                         >  1:197377/1‑138 (MQ=255)
                                                                                     ggTTTTTGGCGGGTGGCGCGGGGTCAAAGGAATAAGTCCATGAAAACCCGCAAAATTAAATTA‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATc                                                       <  2:513952/137‑1 (MQ=255)
                                                                                      gTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAAc                                                                              >  4:991021/1‑114 (MQ=255)
                                                                                       tttttGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCATCTGGAAGCGTTAGATAATCGCtt                                                   >  3:620779/1‑139 (MQ=255)
                                                                                       tttttGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTa                                                   >  4:16516/1‑140 (MQ=255)
                                                                                       tttttGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTa                                                   >  6:1135460/1‑140 (MQ=255)
                                                                                       tttttGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTa                                                   >  3:907180/1‑140 (MQ=255)
                                                                                                 gTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGt                                         >  6:1032923/1‑140 (MQ=255)
                                                                                                  ttGCTGGGGTTCAAAGTAAGAAGTACCTTATAAACCGCAAAATTAATTTA‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTc                                        <  8:23119/140‑1 (MQ=255)
                                                                                                  ttGCGCGGGGTCAAAGTAACAAGTACCTTATAATCCGCAAAATGAAATTA‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTgg                                          <  2:565336/138‑1 (MQ=255)
                                                                                                  tggcgcGGGGTCAAAGTAACAAGTACCTTATAAGCCCCCAAATGAAATTT‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTc                                        <  5:994402/135‑1 (MQ=255)
                                                                                                          ggTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCg                                >  4:405928/1‑140 (MQ=255)
                                                                                                          ggTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCg                                >  5:481756/1‑140 (MQ=255)
                                                                                                            tCAACGTAAGAAGTACCTTATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgca                              <  7:1036293/140‑1 (MQ=255)
                                                                                                               taGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGATGAAGGACGGTAACTGGAGACAGGCAATATCGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                           >  8:820217/2‑140 (MQ=255)
                                                                                                               aaGGAAGAAGTACCTGATAATCCGCCAAATGCAATTT‑ACGCGCCTGCACAACAAAAGCTGCCGGACGGTAACTGGAGCCAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTATGGTCCGTATTCgcagca                           >  4:80431/1‑140 (MQ=255)
                                                                                                               aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                           >  3:148114/1‑140 (MQ=255)
                                                                                                                  gaataaGTACGTTATAATCCGCCAAATGAATTTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGt                        <  8:955939/140‑1 (MQ=255)
                                                                                                                   attctttACCTGAAAACCCCCAAAATGAAATTT‑CCGCGACTGCCCAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATCACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTg                       <  7:1052979/134‑1 (MQ=255)
                                                                                                                   atcaagTACCTCAAATTCCGCAAATTTAAATTA‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGGTTGGTCCGTATTCGCAGCAGGTg                       <  4:776891/137‑1 (MQ=255)
                                                                                                                   aataagAACCTTATAATCCGCAAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTg                       <  6:576812/137‑1 (MQ=255)
                                                                                                                    agaagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTACCTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                      >  5:1007836/1‑140 (MQ=255)
                                                                                                                    agaagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                      >  8:837387/1‑140 (MQ=255)
                                                                                                                    agaagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                      >  7:279912/1‑140 (MQ=255)
                                                                                                                    agaagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                      >  6:908276/1‑140 (MQ=255)
                                                                                                                    agaagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACCGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                      >  6:279377/1‑140 (MQ=255)
                                                                                                                     gaagTACCTGATAATCCGCCAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTACAGGACGGTAACAGGAGACAGGCAATAACGCAACTGGAAGCTATAGATAATCGCTATCCGGTTGGTCAGTATTCGCAGCATGTGCa                     >  6:574209/1‑140 (MQ=255)
                                                                                                                      aagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAg                    >  5:530186/1‑140 (MQ=255)
                                                                                                                      aagTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAg                    >  6:662322/1‑140 (MQ=255)
                                                                                                                       agTACCTTATAATCCGCAAATTTAAATTT‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                   <  3:461887/140‑1 (MQ=255)
                                                                                                                        gTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAg                    >  1:438335/1‑138 (MQ=255)
                                                                                                                        gTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAg                    >  2:1105153/1‑138 (MQ=255)
                                                                                                                            cttaTAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt              <  4:1023060/137‑1 (MQ=255)
                                                                                                                            ctcaTAATCCTCAAATTTAAAATT‑CCGCGCATGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCTATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt              <  5:531516/137‑1 (MQ=255)
                                                                                                                            cTGATTATCCGCCAAATTAAATTT‑ACGCGACTGCACAACAACAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCATCTGGATGCGTTAGATAATCGCTATCCGTTTGTTCCGTATTCGCAGCAGGTGCAGCTGGAt              <  8:977954/140‑1 (MQ=255)
                                                                                                                            cTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAt              >  5:878103/1‑140 (MQ=255)
                                                                                                                            cTGATAATCCGCAAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTgg                <  1:1028460/138‑1 (MQ=255)
                                                                                                                            cTGATAAACCGCAAATTTAAATTA‑ACGCGCCTGCACAACAAACGCTGCAGGACGGTAACTGGAGACCGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTAGCATCAGGTGCAGCTGGAt              <  4:41104/140‑1 (MQ=255)
                                                                                                                             tGATAATCCGCAAAATGAAATGT‑CCGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtc             <  8:861806/140‑1 (MQ=255)
                                                                                                                              gATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTgg                >  1:1064404/1‑136 (MQ=255)
                                                                                                                              gATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTgg                <  2:1064404/136‑1 (MQ=255)
                                                                                                                                   tCCCCCAAATTAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCt       <  4:199930/140‑1 (MQ=255)
                                                                                                                                      gCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACg    >  6:815057/1‑140 (MQ=255)
                                                                                                                                      gCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACg    >  3:704482/1‑140 (MQ=255)
                                                                                                                                      gCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGGTTGGGCCGTATTCGCAGAAGGTGCAGCTGGATCTCATCTACg    >  3:699056/1‑140 (MQ=255)
                                                                                                                                        cAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACg    >  1:873896/1‑138 (MQ=255)
                                                                                                                                        cAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc  <  4:84267/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                            
AGAGAATCTCCCGTATTACATTTTGAGGAAAGTCAAAACGTCATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTT‑ACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCC  >  NZ_CP022686/1370945‑1371220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A