Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 19.2% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC19.2% 173.8 / 6.7 159Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (39/59);  new base C (12/15);  total (75/84)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGAT  >  NZ_CP022686/4440197‑4440471
                                                                                                                                         |                                                                                                                                         
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACa                                                                                                                                         >  4:611504/1‑140 (MQ=255)
  aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCTGCCGCAATATCAGCa                                                                                                                                         >  2:67485/1‑138 (MQ=18)
  aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCCGCAGCCGCAACATCAACa                                                                                                                                         >  1:296856/1‑138 (MQ=255)
   cagcagCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGcc                                                                                                                                      <  5:817180/140‑1 (MQ=255)
      cagcCGCAACACCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACAGCCGCTTGCACCCCAGCCGCAGACTCAGAAGCCGCAAAATCACCAGACGcc                                                                                                                                   >  6:311629/1‑139 (MQ=17)
      cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCCGCAGCCGCAACAGCCGGTAGAACCGCAGCCGCAGTAACCGCAGCCGCAACAACCGGTGGCGCCGCAGCCGCAGTATCCGAAGCGGCAAACTCAGCAGCGGca                                                                                                                                   >  4:384282/1‑140 (MQ=17)
        tccGCAACAACCGGTAGCACCGCAGCATCAATATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGTTGAACCGCAGCCGCAGTAACACCAGCCGCAATATCATCAGACGcaac                                                                                                                                 >  5:81971/2‑140 (MQ=18)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCagcagcagcc                                                                                                                                   >  1:978796/1‑137 (MQ=18)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACACCCGGTTCCGCCGCAGCCGCAGGAGCAGCAGCAGCAAAATCATCAGCCGcc                                                                                                                                   >  1:667815/1‑137 (MQ=18)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAGATCGCCAGACGcc                                                                                                                                   >  1:444609/1‑137 (MQ=18)
         ccGCAACATCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                <  5:1151644/140‑1 (MQ=255)
           gCAACAACCGGTAGCACTGCAGCAGCAATATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTCAACCACAGCCGCAGTATCAGCTGCCGCAGGAGCCGCAGCCGcaacagc                                                                                                                              >  3:632692/1‑138 (MQ=14)
            caacaaCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaacaa                                                                                                                               <  2:907822/138‑1 (MQ=255)
                    ggTATCACCGCAGCAGCAATCTCAGCAGCCGCACCAACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCAc                                                                                                                     <  4:739656/140‑1 (MQ=255)
                      aaGCACCGCCGCAGCAAACTCAGCAGCCGCATCAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCTGTCGCACCGCAGGCGCAGTATCATCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCg                                                                                                                   <  5:400238/139‑1 (MQ=21)
                        gCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                                 <  6:741424/140‑1 (MQ=255)
                             gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagc                                                                                                            <  3:547647/140‑1 (MQ=255)
                             gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGACGCAAAATCATCAGCCGCAACAACCGGATGCACCgcagcagc                                                                                                            >  5:299384/1‑140 (MQ=21)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGGATCCGCAGCGGCATAATCACCAGCCGCAACAGCCGGATGCCCCgcagctgca                                                                                                           >  4:1179704/1‑140 (MQ=18)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGATGCACCgcagcagca                                                                                                           >  6:536010/1‑140 (MQ=255)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGGACCGCAGCCGCAGGATCCGCCGCAGCAATATCCACCTCACCAACCACCCGATGCACAgccggagcg                                                                                                           >  5:955762/1‑139 (MQ=17)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCCCCGCAGCCGCAGAATCAGCAGCCGCAATATCATCAGACGCAACAACCGGGTGCCCCgcagcag                                                                                                             >  2:602846/1‑138 (MQ=21)
                               agcagcaATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTCTCAGCTGCAGCAATATCATCATCCGCCAAATCCCGATGGACCgcagcagc                                                                                                            >  1:1151255/1‑138 (MQ=18)
                                    caATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                                     <  3:400588/140‑1 (MQ=255)
                                    aaaTCTCAGCAGCCGCAACAACCGGAAGCACCGCAGCCGAAGTATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                                     <  6:932303/139‑1 (MQ=255)
                                    aaaTATCAGCAGCCGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGGCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                                     <  3:500264/139‑1 (MQ=255)
                                              agcCTCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAACAACCGGTGGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                           <  3:536104/140‑1 (MQ=255)
                                              agcCGCAACATCCGGTAGCACCGCAGCCGAAGTATCAGCAGCAGCAACAACCGGTGGCACCGCAGCCGCAGTATCATGAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCcac                                                                                             <  2:577965/138‑1 (MQ=25)
                                               gcCGCAACAACCGGTAGCACCGCATCCGCAGTATCCGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCCGCAATATCACGAGCCGCAACAACCGGTGGCACCGCAGCCGCAATCTCAGCCGCCacaac                                                                                          >  5:904206/1‑140 (MQ=21)
                                                catcaacaaCAGGTAGCTCCGCAGACGCAGTATCAGCAGCCGCATCTACCGGTTGCACCGCTGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                           <  2:46436/135‑1 (MQ=25)
                                                   caacaaCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGcc                                                                                      <  7:434319/140‑1 (MQ=255)
                                                     actaacGGTAGCAACGCCTCAGCAGTAGCAGCAGCAGCATCTTCCGGTTGCACCGCTGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCgg                                                                                    <  7:1034344/135‑1 (MQ=25)
                                                                 aCCGCAGAAGCATGATCAGCAGCCTGAAGAACCTGATGCACCGCAGCCGCAGGCTCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACGGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                        <  5:661219/140‑1 (MQ=25)
                                                                   cgcatccgcagTAGCAGCAGCCGCATCAACCGGTTGCACCGCAGCCGCAGTCTCAGCAGCAGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                      <  3:317038/140‑1 (MQ=37)
                                                                   cgcagccgcagTATCAGCAGCCGCAACCACCGGTGGCACCGCAGCTGCAGTATCTTCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                      <  7:426599/140‑1 (MQ=37)
                                                                   cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                      >  3:1027123/1‑140 (MQ=255)
                                                                    gcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTGGCACCGCAGAAGCATTATCAGCAGCCCCAACAGCCGGTTACACCTCagcagc                                                                     >  5:1109738/1‑140 (MQ=25)
                                                                     cagccgcagTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACGCAGAAgcagca                                                                    >  8:734414/1‑140 (MQ=37)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAAAATCCGCAGCCACAACAGCCGGTTACACAGCAGCCGcc                                                                    >  8:331352/1‑139 (MQ=255)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGATGCCCCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTAAGCAGCAGCCGcc                                                                    >  4:702888/1‑139 (MQ=37)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                      >  1:1027541/1‑138 (MQ=255)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAAACAGCAGCCGCa                                                                    >  5:1047884/1‑140 (MQ=255)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGAACCGCAGCCGCAGGATCAGCAGCAGCAAGATCAACAGCCGCGACAACACGTTGCCCCGCAGCAGCAATATCAGCAGCCGCAACAGCCGTCTAAACAGCAGCCGCa                                                                    >  4:102257/1‑140 (MQ=25)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGCCACATCCGGAGGGACCGCAGCAGCATTATCAGAAGCCTCAACAGCCGGTTACAcagcagcagcaga                                                                  >  6:1063221/1‑139 (MQ=21)
                                                                          gcagTATCAGCAGCCGCAACAACCGGATTAACCGCAGCCGCAGTATAAGCAGCCGCAATATCACAAGAAGCAAAAACAGGATGCACCGCAGAAGCAAAATCAGCAGACACAACAGCCGATTCCACAgcagcagcacaatc                                                               >  8:911646/1‑135 (MQ=19)
                                                                                tCAGCCGCAGCAAGAACCGGATGCACCGCAGCCGCAGGATCAGCAGCATCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATcagca                                                           <  2:1068735/138‑1 (MQ=21)
                                                                                ccagcagcagCAACATCCGCTGGAACCTCATCAGCAGTCTCAGCAGGATCAATATCAACATCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCAAAACAGCCGGTTACACAGCAGCAGCAGTATCagcagc                                                         <  4:10423/139‑1 (MQ=18)
                                                                                  agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCg                                                       >  7:244702/1‑140 (MQ=255)
                                                                                    cagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                        <  6:896960/121‑1 (MQ=255)
                                                                                    cagcCGCAACAACCGGATGCACCGCAGCCGCAGTATCTGCAGCAGCAATATCAACATCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCg                                                       <  1:715548/138‑1 (MQ=25)
                                                                                        cGCAACAACCGGTTGCACCGCCGCCGCAGTATCCGCAGCCGCAATATCACCAGCCGCAACAACCGGTGGCACCGCCGCAGCAATATCAGCATCCGCAACAGCCGGTTACCCAGAAGCCGCAGGATCCGCAGCCGCCACAg                                                 >  6:852062/1‑140 (MQ=25)
                                                                                         tcaagtacCGGAAGCTCCCCAGCCGCAGTCTCACCAGCAGCATTATCATCACCCCCATCAACCTGTTGCACCTCAGAAGCAATATCAGCAGCCACAACAGCCGTTTACACAGCAGCAGCAGTATCAGCAGCCGCAACAGc                                                <  4:111191/134‑1 (MQ=16)
                                                                                         gCAACATCCGCTCGCAGCGCAGCCGCAGTCTCAGCAGCAGCAATATCAACAGCCTCAACAACAGGTTGCACCTCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                                <  4:361745/140‑1 (MQ=21)
                                                                                         gCAACAACCGCTTGCACCGCATCCGCAGTCTAAGCAGCCGCCATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                                <  8:81851/140‑1 (MQ=21)
                                                                                          caagcacCGCTAGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                 <  2:378698/133‑1 (MQ=18)
                                                                                          caacacCCGGTTGCACCGCAGCCGCAGCATCAGCAGCAGCATGATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                 <  2:23745/138‑1 (MQ=21)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAAGATCACGAGCAGCAACAACCGGTTGCCCCGCAGGAGCATTATCCGCCGCCCCAACAGCCGGATCCACAGCAGCGGCAGAAGCCGCAGCCGCAagag                                                 >  2:718911/1‑138 (MQ=19)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                 >  1:311841/1‑138 (MQ=255)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                               >  4:616740/1‑140 (MQ=255)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                               <  7:825366/140‑1 (MQ=255)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCCCCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCCGCCGCAACAg                                                 >  1:593535/1‑138 (MQ=255)
                                                                                            accacCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCgg                                             <  5:1161135/137‑1 (MQ=255)
                                                                                             catccGGTTGCACCGCAGCCGCATCATCAGCATCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGt                                            <  3:1162085/137‑1 (MQ=255)
                                                                                             catccGGATGCACCGCAGCCTCATGATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACATCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCg                                              <  2:164736/135‑1 (MQ=21)
                                                                                                    aagCACCGCAGCCGCAGACTCCGGAGCAGCATTATCACCAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                                     <  8:1042911/138‑1 (MQ=18)
                                                                                                     cgCACCGCAGCAGCAGCAACAGCAGCAGCATGATCACCAGCCGCAACAACCGGTAGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                    <  4:9563/139‑1 (MQ=18)
                                                                                                     agCACCGCAGCCTAAGCATCAGCAGCAGCAATATCATCAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                    <  7:894585/139‑1 (MQ=21)
                                                                                                      gCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                                     >  1:106019/1‑138 (MQ=255)
                                                                                                       cACCGCTGCCGCAGCATCCGCAGCCGCACTATCACCATCCGCCACACCCGCTAGCACCGCAGCATCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCAGCAGAATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                  <  5:491503/140‑1 (MQ=255)
                                                                                                       cACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                                    >  1:1205546/1‑138 (MQ=255)
                                                                                                        aCCGCAGCCGCAGTCTATCCAGCAGCAATATCACCATCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACATCCGGTTACACAGCAGCCGCAGGATCAGCAGCCGCAACAGCCGGTTGTACCACAGc                                 <  6:281779/140‑1 (MQ=18)
                                                                                                         ccgcagcctcatTATAAGCAGCAGCAATCTCATCAGCCTCAACAACCGGTTGCACCTCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                <  6:190979/140‑1 (MQ=18)
                                                                                                         ccgcagccgcagTATCAGCAGCCGCAAGATCCACAGCCGCAACAACCGTTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGGATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                <  3:1100025/140‑1 (MQ=21)
                                                                                                         ccgcagccgcagCATCAGCAGCCTCAATATCACCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                  <  2:901127/138‑1 (MQ=255)
                                                                                                         ccgcagccgcagCAGCAGCAGCCGCAATATCACCAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                                <  8:561046/140‑1 (MQ=255)
                                                                                                            cagccgccgGTTCCGCAGCCGCATTTTCATCACGCGCAACCAACGGTTGCAACGCAGCAGCAATATCAGCAGCCTCAACAGCCGGGTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGGACCACAGCCGCa                             <  7:507988/140‑1 (MQ=17)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                             >  7:518087/1‑140 (MQ=255)
                                                                                                              gcctcagGATCAGCAGCCGCAATATCAACAGCTGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                           <  6:489547/140‑1 (MQ=255)
                                                                                                              gccgctcTATAAGCATCAGCAATATCATCAGGCGCAACAACCGGTTGCACCGCAGCAGCATTATCTGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                           <  3:1174205/140‑1 (MQ=18)
                                                                                                              gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                             >  1:220945/1‑138 (MQ=255)
                                                                                                              gccgcagGCTCAGCAGCCGCAATATCATCAGCCTCAACAACCGCATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                           <  4:442754/140‑1 (MQ=21)
                                                                                                              gcagcagGATCAGCTGCAGCAATATCAACAGCCGCATCAACCGGTTGCACCGCAGGAGCAATATCAGCAGCCACCACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                           <  8:959993/137‑1 (MQ=18)
                                                                                                               cagaagTATCAGCAGCCGCATGATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAg                            <  1:679021/134‑1 (MQ=255)
                                                                                                                  cagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGGTACACAGCAGCCGCAGTATCCGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc                       >  3:1220080/1‑140 (MQ=255)
                                                                                                                   attctCAGCAGCCGCAATATCACCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                      <  8:794514/136‑1 (MQ=255)
                                                                                                                   agtcgcagcagCCGTAATCTCCTCAGCCGCAACAACCGCTTGCAACTCAGCATCATTATCAGCAGCCACAACAGCCGGTTACACAGCCGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGCCGCAGGATAcc                      <  6:60303/135‑1 (MQ=18)
                                                                                                                   agcctCAGCAGCCGCAATATCATCAGCCGCACCAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                      <  8:345110/136‑1 (MQ=255)
                                                                                                                   agTATCAGCATCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                      <  5:383083/140‑1 (MQ=255)
                                                                                                                     caTCCGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                      <  2:401402/137‑1 (MQ=255)
                                                                                                                           cagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt                <  1:897280/138‑1 (MQ=255)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCTATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc            >  7:1087435/1‑140 (MQ=255)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACTGCCGCAGGAGACCCTGCTTCATc            >  4:309503/1‑140 (MQ=255)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc            >  6:238818/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTCTACCACAGCCGCAGGATACCCTGCTTCATc            >  1:564488/1‑138 (MQ=255)
                                                                                                                                gCAATATCAACAGCCGCAACAACCGGTTTCACCGCAGAAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGACGCAGTATCATCAGACGCAACAGCCGGTGGTAACAAAGCCGCAGGATACACTGCTTCATcc           >  1:253653/1‑138 (MQ=255)
                                                                                                                                 cAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg          >  1:123127/1‑138 (MQ=255)
                                                                                                                                 cAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCCGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATACgag        >  8:1004682/1‑138 (MQ=255)
                                                                                                                                     atCAACAGCCGCAACAACCGGTTGCACCGCTGCAGCAATATCAGCAGCCACATCAGCCGGTTACACAGCCGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg    <  3:1100510/140‑1 (MQ=255)
                                                                                                                                       cAACATCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGAt  <  5:543908/140‑1 (MQ=255)
                                                                                                                                       cAACAGGCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg    <  2:288873/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGAT  >  NZ_CP022686/4440197‑4440471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A