Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,334 A→C 21.1% Q797H (CAA→CAC ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3340AC21.1% 142.2 / 7.3 146Q797H (CAA→CACftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (50/46);  new base C (10/14);  total (80/66)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440197‑4440473
                                                                                                                                         |                                                                                                                                           
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTGCACCGCTGCCGCTTTATCAGCAGACGCAACAACCGGTTGCACCGCAGCCGGAGTATCAGCAGCCGCAATATCAACa                                                                                                                                           >  5:384629/1‑140 (MQ=21)
gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACAGCAGCCGCAGTATCAGCACCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATaacaacc                                                                                                                                           >  2:538635/1‑139 (MQ=255)
 tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTGCACAGCACCCGCAGTATCACCAGCCGCAATATCACCAg                                                                                                                                          >  5:823497/1‑140 (MQ=21)
    agcagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                                         >  3:558713/1‑138 (MQ=255)
      cagcCGCAACAACAGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACAGCAGCCGCAGTCTCAGCAGCCGCAACCACCGGTTGCGCCTCAGCCGCCGGAACAGCAGCCGCAATATCAACAGCCGcc                                                                                                                                     >  6:856465/1‑139 (MQ=18)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTCGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCTCAGCCGCAGTATCAGCAGCCGCAATATCCACAGCCGcc                                                                                                                                     >  3:157048/1‑137 (MQ=255)
        gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGca                                                                                                                                     >  3:300745/1‑138 (MQ=255)
         ccGCAACAACCTGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                  <  5:912294/140‑1 (MQ=255)
         ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCGGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                  <  6:990093/140‑1 (MQ=255)
         ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                                  <  6:939857/140‑1 (MQ=255)
         ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCCACAGCAGcaaca                                                                                                                                  >  6:663953/1‑140 (MQ=255)
          cGCAACAACCGGTAGCACCGCAGCCGCAATATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCCCCACAGCCGGAGGATCCGCAGCAGCATCATCAGCAGCCGccac                                                                                                                                   >  4:1048924/1‑138 (MQ=11)
           gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCATCAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGccaca                                                                                                                                  >  4:513959/1‑138 (MQ=255)
           gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAACAACCGGTTGCACCTCAGCCGCAGTATCAGCAGCGGCATAATCAGACGCCGccacagc                                                                                                                                >  2:76138/1‑138 (MQ=17)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACATCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCACAGCCGCAGGATCAGCAGCAGCAAAATCAACAGACCCaacaac                                                                                                                                >  4:918676/1‑138 (MQ=18)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGCACAGCAGCCGCAGTATCAGCAGCCGCAACATCCGGATGAACCCCAGCCGCAGGATCAGCAGCGGCAAAATCAGCAGCAGccacatc                                                                                                                                >  4:248464/1‑136 (MQ=14)
             aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAAAAACCGGTTGCACCGCAGCCGCAGAATCAACAGCCTCAAAATCCTCAGACGcaacagc                                                                                                                                >  4:475641/1‑136 (MQ=18)
                       aGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCTCAGCCGCAGTATCAGCAGCCGCAAAATCACCAGCCGCAAAAACCGGAAGGACCgc                                                                                                                    >  6:974448/1‑140 (MQ=18)
                          aCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCCCCGGTTGCCCCGCAGCCGCAGTATCAGCAGCCGCAAAATCCGCAGCCGCAACACCCGGTAGCCCCgcagc                                                                                                                 >  6:223037/1‑140 (MQ=18)
                          aCCGCAGCAGCAATATCAGCACCCGCAACAACCGGAACCACCGCAGCCGCAGTCTCAGCAGCCGCAACAACCGCTATCACCGCAGCCGCAGTATCAGCATCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                                 <  8:737247/140‑1 (MQ=25)
                           ccGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGTTGCACCgcagca                                                                                                                >  7:114559/1‑140 (MQ=255)
                             gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGACGCCACAACCGGTTGCCCCgcagctgc                                                                                                              >  7:191972/1‑140 (MQ=21)
                             gcagcagcaATATCAGCAGCCGCAACAACCGGTAGAACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCATTATCAGCAGCCTCAAACTCAGAAGCCGCAAAAACCGGTAGCCCCtccgcagc                                                                                                              >  7:695164/1‑140 (MQ=18)
                              cagcagcaATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACAGCCGGTTGCACCTCAGCCGCAGGATCAGCAGCAGCAATATCAGCAGCCGCAACACCCGGATCCACCtccgcag                                                                                                               >  4:598973/1‑138 (MQ=18)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGAGGCACCgcagcag                                                                                                               >  3:1075638/1‑138 (MQ=255)
                              cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATTATCATCAGAGGCAACATCCGGTTGCACCgcagaagca                                                                                                             >  7:325995/1‑140 (MQ=18)
                                gcagcaATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCTGCCGCAAGATCAGCAGCCGCAACAACCGGTTGGACCgcagcagca                                                                                                             >  3:944991/1‑138 (MQ=21)
                                gcagcaATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCACCAGCCGCCACAACCGGTGGAACCgcagctgca                                                                                                             >  4:217899/1‑138 (MQ=18)
                                       tatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcag                                                                                                      <  4:749331/138‑1 (MQ=255)
                                             cagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCCGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCCACAACCGGTTGCACCGACGCATCAATATCAGCAGCccca                                                                                              >  2:155831/1‑140 (MQ=21)
                                              agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGATGCACCTCAGCCGCAGTATCAGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGACacaa                                                                                             >  2:776458/1‑140 (MQ=25)
                                              agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  5:864270/140‑1 (MQ=255)
                                                ccGGAACTACCGCTAGCACCGCAGGCGGAGTATATGCAGCCGCATGATCCGGTGGCACCTCAGCCGCTGTATCCGCAGCCGCATTATCAACAGCCTCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  4:1107729/138‑1 (MQ=18)
                                                ccGGAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  3:827694/138‑1 (MQ=255)
                                                ccGCATCAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                           <  1:806118/140‑1 (MQ=255)
                                                ccGCATCAACAGGAAGCTCAGCAGCCGCAGTCTCCGCAGCAGCAACAACCGGATGCACCGCAGCCGCAGCCACAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                             <  4:595327/138‑1 (MQ=21)
                                                 cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTTACCGCAGCCGCAAACTCAGCAGCCGCCACAGCCGGAGGCCCCGCAGCAGCAGAAGCCGCCGCCCCAACAg                                                                                          >  8:828373/1‑140 (MQ=12)
                                                  taatcaacCGGCACCCACGGAGCCGCAGAATCAGGAGCCGCAACTACCGGTCCCACCTGATCCGCAGTATCATCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                         <  6:384629/136‑1 (MQ=21)
                                                  gCAACAAACGGTAGCACCGCAGACGCAGTATCAGCAGGCGCAACTACCTGTTGCTCCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACTACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                                         <  1:1103412/140‑1 (MQ=37)
                                                           cgTATCACCTCAGCAGAACTATCAGCAGGCGCAACAACCGCGAGCAACGCACACTCAGTAAAACCAGCCGCAATAACACCAGCCGCAACAACCGGAATCACCGCAGCAGCAATATCATCAGCCACAACAGCCGGTTaaac                                                                                <  8:473087/139‑1 (MQ=12)
                                                            gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                                >  8:115262/1‑139 (MQ=255)
                                                            gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                                <  7:115262/139‑1 (MQ=255)
                                                                cACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCCCCGCAGCAGCAATATCAGCCGACACCACAGCCGGGTaccccgc                                                                             >  4:387712/1‑133 (MQ=25)
                                                                 aCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                          <  7:913371/140‑1 (MQ=255)
                                                                  ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  8:404488/140‑1 (MQ=255)
                                                                  ccgcagcagctgTCACAGCTGCCTCTCCAACCGGTAGCACCGCAGCCGCTGTATCCGCAGCCGCAATATCCACAGCCGCACAAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  1:686705/140‑1 (MQ=25)
                                                                  ccgcagcagcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  8:404491/140‑1 (MQ=255)
                                                                  cagcagcctaagTCTCTGCAGCAGCAACATCAGGTGGCACCCCATCATAAGTATCAGCAGCCGCAATATCAACAGCCGCAACTACCGTTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                                         <  8:27585/138‑1 (MQ=21)
                                                                   cgcagccgcagTCTCAGCAGCCGCAACAACAGCAGGCACCGCAGCCTCAGTCTCATCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  8:146033/140‑1 (MQ=25)
                                                                   cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCTGCCg                                                                        >  1:679050/1‑140 (MQ=255)
                                                                   cgcagccgcagTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  1:203398/140‑1 (MQ=255)
                                                                   cgcagccgcagTATCAGCAGCAGCATCAACCGGATGCACCGCAGCAGCAGTATCAGCAGCAGCATTATCATCAGCCGCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                                        <  6:437249/140‑1 (MQ=37)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAAGAGCCGCAACAACCGGTAGCACCGCAGAAGCAATATCAGCAGCCACCACAGCCGGGTACACAGCAGCCGcc                                                                      >  8:434156/1‑139 (MQ=37)
                                                                     cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCCAAGCAGCAGTATAAGCAGACGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATAACAGCAGCCACAACAGCCGGATACACAGCAGCCGCa                                                                      >  6:678335/1‑140 (MQ=37)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCTCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTCCACCGCAGCAGCAATATAAGCAGCCACAACAGCCGGTTACACAGCCGCCGCa                                                                      >  3:917123/1‑138 (MQ=255)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                                      >  3:542329/1‑138 (MQ=255)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGACACAACAGCCGGTTACACAGCAGCCGCa                                                                      >  3:16829/1‑138 (MQ=255)
                                                                       gccgcagTATCAGCAGCCGCAACAACCGGTTGAACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCGGCCACAACCGGTAGCCCCGCAGCTGCAATATCAGCAGCCACAACAGCCGTTTAAGCATCAGCCgga                                                                      >  4:111984/1‑136 (MQ=21)
                                                                                      gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTCCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACACATCAGCCGCAGAATCCGCAGCCGCAAc                                                     >  2:1137989/1‑140 (MQ=37)
                                                                                       ccGCAACAACCGGTTGCACCGCTGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                                      <  3:138740/138‑1 (MQ=255)
                                                                                       ccGCAACAACCGGATGCTCCGCAGCCGCAGGCTCAGCAGCAGCAATATCAACAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                                      <  3:120728/138‑1 (MQ=21)
                                                                                       ccGCAACAACCGCATGCACCGCAGCCGCACTATCTGCAGCCGCAATATCAACAGCCTCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                                    <  2:1015740/140‑1 (MQ=21)
                                                                                        cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCCACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACCCAGCAGCCGCAGGATCAGCCGCCGCAACAg                                                   >  6:450248/1‑140 (MQ=25)
                                                                                        cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                                   >  8:47533/1‑140 (MQ=255)
                                                                                         gCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                                    <  3:355957/138‑1 (MQ=255)
                                                                                         gCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGACACCACAGCCGGTTACACAGCAGCAGCAGTAACCGCAGCCGCAACa                                                    >  4:1069970/1‑138 (MQ=37)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGGAGCCGCAGCCGCAACAg                                                   >  4:158887/1‑138 (MQ=37)
                                                                                          caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCAGCAGCCACAACAGCCGGTTAAACCGCAGCGGCAGTATCAGCAGCCGCCACAg                                                   >  4:795491/1‑138 (MQ=38)
                                                                                                 cGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                          <  5:365911/108‑1 (MQ=255)
                                                                                                   gTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcc                                        >  1:373226/1‑140 (MQ=255)
                                                                                                   gTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGGTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcc                                        >  8:249280/1‑140 (MQ=255)
                                                                                                    ttGCTCCGCTGCCGCAGTCTAAGTAGCATGAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCTGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                                       <  8:80378/140‑1 (MQ=18)
                                                                                                    aagaaCCGCAGCCGCAGTATAAGCAGCAGCAAGATCACCACCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAc                                         <  4:966624/134‑1 (MQ=21)
                                                                                                       cACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  7:226515/140‑1 (MQ=255)
                                                                                                       cACCGCAGCAGCAGGATCAGCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                                    <  1:41814/140‑1 (MQ=255)
                                                                                                          cgcagccgcacTCTCAGCAGCATCAATATCAACAGCCGCAACATCCGGAAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                                 <  7:905189/140‑1 (MQ=18)
                                                                                                            cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGAAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCTGTTGTACCACAGCCGcc                               >  6:464482/1‑139 (MQ=255)
                                                                                                              gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                             <  5:663439/140‑1 (MQ=255)
                                                                                                               ccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCTCAGGa                            <  1:557018/140‑1 (MQ=255)
                                                                                                                   agTATCAGCAGGAGCATTATCAGCAGCCTCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  3:842376/138‑1 (MQ=18)
                                                                                                                   agTATCAGCAGCCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  7:923378/140‑1 (MQ=255)
                                                                                                                   agTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  4:924346/138‑1 (MQ=255)
                                                                                                                   agTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCAACGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  1:1053875/140‑1 (MQ=255)
                                                                                                                   agTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                          <  3:522233/138‑1 (MQ=255)
                                                                                                                    gTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGAACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc                         >  4:238959/1‑138 (MQ=255)
                                                                                                                     tacaagcagcCGCATTATCACCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc                        <  4:996646/134‑1 (MQ=18)
                                                                                                                           cagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa                >  6:304857/1‑140 (MQ=255)
                                                                                                                             tccGCAATATCAACAGCCGCAACAACCGGTTGAACCGCAGCCGCAATATCAGAAGCCACAACCGCCGGTTACACCGCAGACGCAGTATCAGCAGCCGCAACAGACGGTTGTACCAACGCCGAAGGCTAACCTTCTTAATc              >  2:1076342/2‑140 (MQ=17)
                                                                                                                             gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCCCAACAGCCGGTTGTACCACAGCCGCAGGCTCCCCTGCTTCATc              >  1:1022290/1‑140 (MQ=255)
                                                                                                                              ccGCCATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATcc             <  1:600675/140‑1 (MQ=255)
                                                                                                                               cGCAATATCCCCAGCCCCAACACCCGGTCGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGATGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  1:219663/140‑1 (MQ=18)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACCGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  2:112879/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACACTGCTTCATCCg            >  8:1039451/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  2:823174/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  2:927133/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            >  1:658735/1‑140 (MQ=255)
                                                                                                                               cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGGACCACAGCCGCAGGATACCCTGCTTCATCCg            >  2:743416/1‑140 (MQ=255)
                                                                                                                               atcAAGATCAACAGGCTCAACAACTGGTTGCACCGCAGCAGCAATTTCAGCAGCCACAACTGCCGGTTTCACAGCATCAGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  2:1122916/138‑1 (MQ=21)
                                                                                                                               agCAATATCAACAGCCGCAACACCCGGTGGCACCGCAGCAGCACTATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg            <  8:786223/139‑1 (MQ=255)
                                                                                                                                     atCAACAGCCGAACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTg      <  8:42727/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCGCTGTTGATGC  >  NZ_CP022686/4440197‑4440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A