Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F35 I0 R1 157 6985.9 8006972 98.3% 7870853 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,412,284 A→C 16.7% W241G (TGG→GGG)  ybjT ← SDR family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,412,2840AC16.7% 261.9 / 8.9 159W241G (TGG→GGG) ybjTSDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (91/34);  new base C (2/29);  total (93/66)
Fisher's exact test for biased strand distribution p-value = 6.90e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CGATCAGCCGTTGTGGGATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCGGAT  >  NZ_CP022686/4412149‑4412415
                                                                                                                                       |                                                                                                                                   
   tCAGCCGTTGTGGGATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAACCCACACCGAAATCCAGCGGGTg                                                                                                                              >  7:919791/1‑140 (MQ=255)
   tCAGCCGTTGTGGGATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACAACACCGAAATCCAGCGGGTg                                                                                                                              >  8:67001/1‑140 (MQ=255)
    cAGCCGTTGTGGGATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGt                                                                                                                               >  2:932591/1‑138 (MQ=255)
         gTTGTGGGAGGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCGGGCGGTGGTGGGCGGTACTTAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGa                                                                                                                          <  4:853026/138‑1 (MQ=255)
             tgGGATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAgggg                                                                                                                      >  4:710676/1‑138 (MQ=255)
              gggATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAggggg                                                                                                                     >  4:506480/1‑138 (MQ=255)
               ggATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATg                                                                                                                  >  7:421806/1‑140 (MQ=255)
                 aTGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACCGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATg                                                                                                                  >  1:1022149/1‑138 (MQ=255)
                   gAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATggg                                                                                                                >  1:881463/1‑138 (MQ=255)
                    aGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATgggg                                                                                                               >  1:419028/1‑138 (MQ=255)
                      cgcACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCCCATTAAGAAACCCCACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAg                                                                                                             >  1:422807/1‑138 (MQ=255)
                      cgcACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACCCCGAAATCCAGCGGGGGGGGAGGGGGATGGTGAg                                                                                                             >  4:739367/1‑138 (MQ=255)
                      cgcACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCTGGTGGGGAGGGGGATGGGGAg                                                                                                             >  3:828578/1‑138 (MQ=255)
                      cgcACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAg                                                                                                             >  3:875104/1‑138 (MQ=255)
                      cgcACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGAGGTGGGCGGTTCTGCAGTAATCACATTAAGAAACCCCTCCGAAATCCAGCGGGTggggagggggagggggag                                                                                                             >  5:307265/1‑138 (MQ=255)
                        cACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGAGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACTCCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCa                                                                                                           >  4:915773/1‑138 (MQ=255)
                             tgcgcGGTGTCATCCGCCAGCAGATCGTGTTTCCGCCCCTGAATCACCGCCGTGGGGGTGGTGGGCGGTACTGAAGTAATAACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGCGGGGGATGGGGAGCAACCAg                                                                                                      <  4:343396/137‑1 (MQ=255)
                             tgcgcGGTGTCATCCCCCAGCAGATCGTGTTGCACCCCCTCAACCACCCCCCGGGGGGTGGTGGGCGGTACTGAAGTAATCACATTAACAAACCACACCCAAATCCAGCGGGGGGGGAGGGGGATGGGGAGCAACCAgcg                                                                                                    <  7:215365/139‑1 (MQ=255)
                                gcgGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAgcgg                                                                                                   >  5:287765/1‑138 (MQ=255)
                                 cgGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAgcggc                                                                                                  >  5:123505/1‑138 (MQ=255)
                                   gtgtCATCCGACAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGCAGTAATCACATTAAGAAACCACACCGAAAGCCAGCGGGTGGGGAGTGGGATTGGTAGCAACCAGCGGCGCtt                                                                                              >  8:540109/1‑140 (MQ=255)
                                     gtCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACCTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGCGCACCCAGCGGCGCtt                                                                                              >  3:254474/1‑138 (MQ=255)
                                     gtCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCtt                                                                                              >  5:950637/1‑138 (MQ=255)
                                           cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTcc                                                                                      >  8:762799/1‑139 (MQ=255)
                                           cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGGGGGGAGGGGGATGGGGAGCACCCAGCGGCGCTTACCGCt                                                                                        >  3:404778/1‑138 (MQ=255)
                                            cccAGCAGAGCGTGTCTCAGCCCCTGAATCACCGCCCTGGGGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTc                                                                                       <  5:492752/137‑1 (MQ=255)
                                             ccAGCATAGCGTGTTTCAGCCCCGGAATCAACCCCCTGGCGGTGGGGGGCGGTACTGAAGTAATCACATTAACAAACCACACCCAAATCCAGCGGGGGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCAcc                                                                                    <  8:814686/140‑1 (MQ=255)
                                              cagcagATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCg                                                                                   >  7:944230/1‑140 (MQ=255)
                                                gcagATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCg                                                                                   >  2:116277/1‑138 (MQ=255)
                                                gcagATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGAGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCCCCCAGCGGCGCTTACCGCTGACCg                                                                                   >  6:89168/1‑138 (MQ=255)
                                                  agATCGTGTTTCAGCCCCTCAATAACCGACCTGGCGGTGATGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCTCAAACCAGCGTGTGGGGAGCTGGATGCGGAGCAACCAGCGGCGCTTACCGCTCACCGcc                                                                                 >  5:129462/1‑138 (MQ=255)
                                                     tggggtTTCAGCCCCTTAATCAAGGCAGGGGGGGGGGTGGGCGTTACTTAAGTAATAACATTAAGAAACCACACCGAAATCCAGCGGGGGGGGTGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATaaa                                                                            <  7:511649/136‑1 (MQ=255)
                                                          tttCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATg                                                                         >  2:772149/1‑138 (MQ=255)
                                                             cAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTc                                                                      >  2:240807/1‑138 (MQ=255)
                                                              cgCCCCTTATTCACCGCCGGGGGGGTGGTGGGCGTTACTGAAGAAATAACATAAAGAAACCACACCGAAAACCAGGGGGTGGGGTGGGGTATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa                                                                     <  3:875857/137‑1 (MQ=255)
                                                              cgCCCCTGAATCAACGCCCGGGCGGGGGGGGGCGGTACTGAATTAATCACATAAAGAACCCCCACCGAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCaaa                                                                   <  7:786382/139‑1 (MQ=255)
                                                              atccccTGCATCAACGCCCTGGGGGTGGTGGGCGTTACTGAAGTAATCACATTAAGAAACCACACCGAACTCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa                                                                     <  2:270810/136‑1 (MQ=255)
                                                              aGCCCCTGATTCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACCCGGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa                                                                     <  6:382614/138‑1 (MQ=255)
                                                                 cccTGAAGCAACGCCCGGGGGGTGGGGGGCGGTACTTAAGTAATCACATTAAGACACCCCACCGAAATCCCGGGGGGGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTg                                                                <  8:349455/140‑1 (MQ=255)
                                                                  ccTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACCCCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTAAAACt                                                                 >  5:436882/1‑138 (MQ=255)
                                                                  ccTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAACTCCAGCGGGTTGGGAGGGGGAGGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTACAAACt                                                                 >  3:230941/1‑138 (MQ=255)
                                                                      aagcAACGCCCTGGGGGTGTGGGGCGGTACTTAATTAATCACATTAACAAACCACACCTCAATCCCGCGGGGGGGTAGGGGTTTGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTg                                                             <  1:119317/135‑1 (MQ=255)
                                                                      aagcAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCt                                                           <  8:189033/137‑1 (MQ=255)
                                                                      aaTCACCGACTTGGGTGTTGTGGGCGTTAGTGAAGTAATAAAATAAAGAAACCACACAGAAATCCTGCGGGTGGGGTGGGGTATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCt                                                           <  7:846696/140‑1 (MQ=255)
                                                                        ccAACGCCCGGGCGGTGGGGGGCGGTACTGAAGTAATCACAATAAGAAACCACACCGAAATCCAGGGGGTGGGGTGGGGGAGGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCt                                                           <  5:155529/137‑1 (MQ=255)
                                                                        acaccGCCCTGGGGGGGGGGGGCGGTACTGAAGTAATCAAATTAAGAAACCACACCGAAATCCCGCGGGTGGGGAGGGGTATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCt                                                           <  1:60140/134‑1 (MQ=255)
                                                                           aCGCCCTGGCGGTGGTGGGCGGTACTGAAGAAATCACATTAAGAAACCACACCGAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGAt                                                        <  4:472783/138‑1 (MQ=255)
                                                                            cGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATa                                                       >  2:357845/1‑138 (MQ=255)
                                                                              cccTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATaaa                                                     >  5:767589/1‑137 (MQ=255)
                                                                                gtgggggtgggggGCGGTACTGATGTAATCACATTAAGAAACCACACCGAAATCCCGCGGGTGGGGAGGGGGAGGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAg                                                 <  8:421806/139‑1 (MQ=255)
                                                                                gtGGGGGGGGTGGGGGTTACTGTATTAATCAAATTACCAACCCACACGCATATCCCGCGGGGGGGGTGGGGTATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCAATAAAATGTTCAAACTGTTGCTGATAACTg                                                   <  1:334797/137‑1 (MQ=255)
                                                                                gtGGCGTGGGTGGGCGGTACTGAAGTAATAACATTAACAACCCCCACCGAAATCCCGCGGGTGGGGAGGGGGAGGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                   <  2:885998/137‑1 (MQ=255)
                                                                                cTGGGGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCTAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                   <  3:909451/138‑1 (MQ=255)
                                                                                cTGGCGGTGGGGGGCGGTACTGAAGTAAACACATTAACAAACCCACCCGAAATCCCGGGGGTGGGGTGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                   <  5:692259/138‑1 (MQ=255)
                                                                                  ggCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAg                                                 >  6:200525/1‑138 (MQ=255)
                                                                                        ggtggGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTg                                         >  7:27016/1‑140 (MQ=255)
                                                                                           gggCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTgg                                        >  5:491363/1‑138 (MQ=255)
                                                                                            ggCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGt                                       >  3:702798/1‑138 (MQ=255)
                                                                                               ggTACTTAAGTACTAAACTAAAGAAACCCCGCCGAATTCCCGGGGGGGGGGTGGGGGTTGGGGAGCAACCAGCGGCGCGTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCcg                                    <  1:303032/138‑1 (MQ=255)
                                                                                                   cTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCcggcgg                                >  5:932170/1‑138 (MQ=255)
                                                                                                   cTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCt                              >  8:868940/1‑140 (MQ=255)
                                                                                                    tGAATTAATCAAATTAATACACCAATCCGAAATCCAGCGGGGGGGGTGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCggcggc                               <  3:915773/138‑1 (MQ=255)
                                                                                                    tGAAGTAATCACATTAATAAACCACACCCAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCggcggc                               <  6:114563/138‑1 (MQ=255)
                                                                                                    tGAAGTAATCAAATTAAGAAACCACACCGAAATCCAGCGGGGGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCggcggc                               <  6:276491/138‑1 (MQ=255)
                                                                                                    ggAAGTAATAACATTAAAAAACCCCACCTAAATCCCGCGGGTGGGGAGGGGGAGGGGGAGCAACCAGCGGCGCTTACCGCTCACAGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCggcggc                               <  3:567005/137‑1 (MQ=255)
                                                                                                      aaGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCtt                             <  6:471960/138‑1 (MQ=255)
                                                                                                           atccaaTTAAGAAACCACCCCTAACTCCAGGGGGGGGGGTGGGGCTTGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      <  7:374427/135‑1 (MQ=255)
                                                                                                           aTCACATTAAGCAACCACACCGAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACAGCCATAAATTGGTCAAACTGTTGCTGATAACTGCTCACTTCGGGTCCGGCGGCTTCGAAGAt                      >  8:939939/1‑140 (MQ=255)
                                                                                                           aTCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      >  8:637192/1‑140 (MQ=255)
                                                                                                           aTCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      >  7:16303/1‑140 (MQ=255)
                                                                                                           aTCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      >  7:702212/1‑140 (MQ=255)
                                                                                                             cacaTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCTCTTCTGGTCCGGCGGCTTCGAAGAt                      >  5:951383/1‑138 (MQ=255)
                                                                                                             cacaTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      >  4:245498/1‑138 (MQ=255)
                                                                                                             cacaTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      >  4:143242/1‑138 (MQ=255)
                                                                                                             cacaTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGAt                      >  2:831242/1‑138 (MQ=255)
                                                                                                             cacaTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGATGCTGATAACTGAGCACTTCTGGTCCGGCGGCTGCGAAGAt                      >  6:258611/1‑138 (MQ=255)
                                                                                                             cacaTTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCAATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCGTCGAAGAt                      >  2:949454/1‑138 (MQ=255)
                                                                                                                  tAAGAACCCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAACCTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGgt                 >  5:149327/1‑138 (MQ=255)
                                                                                                                  tAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGgt                 >  3:660402/1‑138 (MQ=255)
                                                                                                                    aGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGgtgt               >  5:933397/1‑138 (MQ=255)
                                                                                                                     ccccccACCCCGAACCCCAGGGGGGGGGGCGGGGGAGGGGGAGCCACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAACCTGTGGCTGATAACTGAGCACTGCTGGTCCGGCGGCTTCGAAGATGCGGGGtt              <  4:600588/134‑1 (MQ=255)
                                                                                                                     caaaCCACACCCAAATCCAGGGGGGGGGGTGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGtt              <  3:636574/137‑1 (MQ=255)
                                                                                                                          cacaCCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  7:637372/1‑140 (MQ=255)
                                                                                                                          cacaCCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  8:585273/1‑140 (MQ=255)
                                                                                                                          cacaCCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  7:550475/1‑140 (MQ=255)
                                                                                                                            ccccGAAATCCCGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       <  6:694477/136‑1 (MQ=255)
                                                                                                                            ccccGAAATCCCGCGGGGGGGTAGGGGTATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       <  6:545494/136‑1 (MQ=255)
                                                                                                                            cacCGACTTCCCGGGGGGGGGGTGGGGTATGGGGAGCATCCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAATCTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       <  6:944443/138‑1 (MQ=255)
                                                                                                                            cacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  5:578021/1‑138 (MQ=255)
                                                                                                                            cacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  1:905948/1‑138 (MQ=255)
                                                                                                                            cacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  3:1009888/1‑138 (MQ=255)
                                                                                                                            cacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGc       >  6:476129/1‑138 (MQ=255)
                                                                                                                              cctaaaTCCCGCGGGTGGGTAGGGGGATGGGGAGCAACCCGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCg     <  5:67360/135‑1 (MQ=255)
                                                                                                                              cctaaaTCCCGCGGGTGGGGCGGGGGTGGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCg     <  2:113085/135‑1 (MQ=255)
                                                                                                                              cccaaaCCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGCGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCg     <  5:189022/135‑1 (MQ=255)
                                                                                                                              ccGAAATCCCGCGGGGGGGGCGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAACTGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCGga   <  7:847936/140‑1 (MQ=255)
                                                                                                                              ccGAAATCCCGCGGGGGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCg     <  3:130515/138‑1 (MQ=255)
                                                                                                                              ccGAAATCCCGCGGGGGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCg     <  5:752811/138‑1 (MQ=255)
                                                                                                                              ccGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCg     <  6:287765/138‑1 (MQ=255)
                                                                                                                               cGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGACCGGCGTCTTCGAAGATGCGGTGTTCGCAGGCCgg    >  6:544513/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CGATCAGCCGTTGTGGGATGAGCGCACGTAGCGCGGTGTCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACTGAAGTAATCACATTAAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAGCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACTTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCAGGCCGGAT  >  NZ_CP022686/4412149‑4412415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A