Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,324 C→A 15.5% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3240CA15.5% 245.2 / 4.0 149P794Q (CCG→CAG) ftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (55/62);  new base A (11/19);  total (68/81)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATC  >  NZ_CP022686/4440185‑4440461
                                                                                                                                           |                                                                                                                                         
        aCCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATc                                                                                                                                   <  6:977854/140‑1 (MQ=255)
              aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCCGCAGCCGCAACATCAACa                                                                                                                               >  1:296856/1‑138 (MQ=255)
               cagcagCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGcc                                                                                                                            <  5:817180/140‑1 (MQ=255)
                agcagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCATCAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                             <  2:144717/138‑1 (MQ=255)
                  cagcCGCAACACCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACAGCCGCTTGCACCCCAGCCGCAGACTCAGAAGCCGCAAAATCACCAGACGcc                                                                                                                         >  6:311629/1‑139 (MQ=17)
                    tccGCAACAACCGGTAGCACCGCAGCATCAATATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGTTGAACCGCAGCCGCAGTAACACCAGCCGCAATATCATCAGACGcaac                                                                                                                       >  5:81971/2‑140 (MQ=18)
                    gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCagcagcagcc                                                                                                                         >  1:978796/1‑137 (MQ=18)
                    gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACACCCGGTTCCGCCGCAGCCGCAGGAGCAGCAGCAGCAAAATCATCAGCCGcc                                                                                                                         >  1:667815/1‑137 (MQ=18)
                    gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCAGcaac                                                                                                                       >  4:822562/1‑140 (MQ=255)
                    gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAAGATCGCCAGACGcc                                                                                                                         >  1:444609/1‑137 (MQ=18)
                    gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGAAGCCGCAGGATCAGCAGCCGCAATATCAGCAGACGccac                                                                                                                       >  4:322536/1‑140 (MQ=21)
                     ccGCAACATCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                      <  5:1151644/140‑1 (MQ=255)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                        <  2:643289/138‑1 (MQ=255)
                       gCAACAACCGGTAGCACTGCAGCAGCAATATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTCAACCACAGCCGCAGTATCAGCTGCCGCAGGAGCCGCAGCCGcaacagc                                                                                                                    >  3:632692/1‑138 (MQ=14)
                       gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTGGCACCGCAGCCGCAGTATCAGCAGCAGCAAAATCAGAAGACGcaaaatc                                                                                                                    >  4:576068/1‑136 (MQ=18)
                       gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAACAACCGGTTGAGCCAAAGCCGCAGGAACAGCAGCAGCCAACTCAGAAGCCGcaacagc                                                                                                                    >  8:942328/1‑138 (MQ=17)
                        caacaaCCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaacaa                                                                                                                     <  2:907822/138‑1 (MQ=255)
                         aacaacCGGTAGAACCGCAGCAGCAATATCCGCAGCCGCAACAACCGGTAGCCCCGCATCCGCAGGAGCAGCAGCAGCAACATCAGGGTGAACCCCCGCCGCAGTATAAGCAGCAGCAAAATCACCCGCACCaacaac                                                                                                                    >  2:265442/1‑138 (MQ=16)
                                ggTATCACCGCAGCAGCAATCTCAGCAGCCGCACCAACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCAc                                                                                                           <  4:739656/140‑1 (MQ=255)
                                  aaGCACCGCCGCAGCAAACTCAGCAGCCGCATCAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCTGTCGCACCGCAGGCGCAGTATCATCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCg                                                                                                         <  5:400238/139‑1 (MQ=21)
                                    gCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                       <  6:741424/140‑1 (MQ=255)
                                      cccGCAGCAGCAATCTCAGCAGCCGCAACAACCGGTAGCACCGCAGACGCAGGATCAGCAGCCGCAACAACCGGTGGCACCGCAGCCGCAGGATCAGCAGCCGCAATAACAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                     <  5:913026/139‑1 (MQ=21)
                                      aCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                       <  1:495442/138‑1 (MQ=255)
                                         gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagc                                                                                                  <  3:547647/140‑1 (MQ=255)
                                         gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGACGCAAAATCATCAGCCGCAACAACCGGATGCACCgcagcagc                                                                                                  >  5:299384/1‑140 (MQ=21)
                                          cagccgcaATAGCAGCAGCCGCATCAACCGGTAGCACCGCAGCCGCATTATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCTCCGTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagca                                                                                                 <  8:975752/140‑1 (MQ=21)
                                          cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGGATCCGCAGCGGCATAATCACCAGCCGCAACAGCCGGATGCCCCgcagctgca                                                                                                 >  4:1179704/1‑140 (MQ=18)
                                          cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGATGCACCgcagcagca                                                                                                 >  6:536010/1‑140 (MQ=255)
                                          cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCCCCGCAGCCGCAGAATCAGCAGCCGCAATATCATCAGACGCAACAACCGGGTGCCCCgcagcag                                                                                                   >  2:602846/1‑138 (MQ=21)
                                          cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACAGCAGCCGCAGTATCACCAGCCGCAAAATCATCAGCCGCCACAACCGGAAGCAACacagcagca                                                                                                 >  4:1083521/1‑140 (MQ=18)
                                          cagcagcaAAATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTAGCACAGCAGCCGCAGTATACCCACCCGCATAAACAACAGACCCCACAACCGGATGCACCtcagcagca                                                                                                 >  4:391713/1‑140 (MQ=17)
                                           agcagcaATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTCTCAGCTGCAGCAATATCATCATCCGCCAAATCCCGATGGACCgcagcagc                                                                                                  >  1:1151255/1‑138 (MQ=18)
                                                caATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                           <  3:400588/140‑1 (MQ=255)
                                                aaaTCTCAGCAGCCGCAACAACCGGAAGCACCGCAGCCGAAGTATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                           <  6:932303/139‑1 (MQ=255)
                                                aaaTATCAGCAGCCGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGGCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                           <  3:500264/139‑1 (MQ=255)
                                                  atatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagc                                                                                         <  5:1114673/140‑1 (MQ=255)
                                                       agcagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCGGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCTGCATACTCAGCAGccc                                                                                    >  5:475982/1‑139 (MQ=21)
                                                          agcCGCAACATCCGGTAGCACCGCAGCCGAAGTATCAGCAGCAGCAACAACCGGTGGCACCGCAGCCGCAGTATCATGAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCcac                                                                                   <  2:577965/138‑1 (MQ=25)
                                                           gcCGCAACAACCGGTAGCACCGCATCCGCAGTATCCGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCCGCAATATCACGAGCCGCAACAACCGGTGGCACCGCAGCCGCAATCTCAGCCGCCacaac                                                                                >  5:904206/1‑140 (MQ=21)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                               <  3:1008247/140‑1 (MQ=255)
                                                            ccGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                               <  8:802998/140‑1 (MQ=255)
                                                            catcaacaaCAGGTAGCTCCGCAGACGCAGTATCAGCAGCCGCATCTACCGGTTGCACCGCTGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                 <  2:46436/135‑1 (MQ=25)
                                                              gCATCAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                             <  4:973802/140‑1 (MQ=255)
                                                               caacaaCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGcc                                                                            <  7:434319/140‑1 (MQ=255)
                                                                 actaacGGTAGCAACGCCTCAGCAGTAGCAGCAGCAGCATCTTCCGGTTGCACCGCTGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCgg                                                                          <  7:1034344/135‑1 (MQ=25)
                                                                  caacCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGt                                                                         <  3:850345/140‑1 (MQ=255)
                                                                     ccGGAAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTac                                                                      <  3:522047/140‑1 (MQ=255)
                                                                             aCCGCAGACGCAGTATCAGCAGCCGCAACTTCCGGATGCACCGCAGCCTCAGAAACAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                              <  6:173732/140‑1 (MQ=37)
                                                                             aCCGCAGAAGCATGATCAGCAGCCTGAAGAACCTGATGCACCGCAGCCGCAGGCTCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACGGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                              <  5:661219/140‑1 (MQ=25)
                                                                              ccgcagccgcacTAGCTGCAGCCGGAACAACCGGATGCACCGCAGCCGCAGTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                             <  4:272702/140‑1 (MQ=37)
                                                                              ccgcagcagcagTCTCAGCAGCAGCAACAACCGCTCGCACCGCAGCCGAAGAATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                             <  3:1074652/140‑1 (MQ=37)
                                                                               cgcatccgcagTAGCAGCAGCCGCATCAACCGGTTGCACCGCAGCCGCAGTCTCAGCAGCAGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                            <  3:317038/140‑1 (MQ=37)
                                                                               cgcagccgcagTATCAGCAGCCGCAACCACCGGTGGCACCGCAGCTGCAGTATCTTCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                            <  7:426599/140‑1 (MQ=37)
                                                                                 cagccgcagTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACGCAGAAgcagca                                                          >  8:734414/1‑140 (MQ=37)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAAAATCCGCAGCCACAACAGCCGGTTACACAGCAGCCGcc                                                          >  8:331352/1‑139 (MQ=255)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCCGCCGCAATATCAACAGCCGCAACACCCGGTTGCCCCGCAGCAGCAATATCAGCCGCCCCAACAGCCGGTTCCACAGCAGCCGcc                                                          >  7:820314/1‑139 (MQ=37)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                            >  1:1027541/1‑138 (MQ=255)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                            >  1:138587/1‑138 (MQ=255)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAAACAGCAGCCGCa                                                          >  5:1047884/1‑140 (MQ=255)
                                                                                   gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGCCACATCCGGAGGGACCGCAGCAGCATTATCAGAAGCCTCAACAGCCGGTTACAcagcagcagcaga                                                        >  6:1063221/1‑139 (MQ=21)
                                                                                      gcagTATCAGCAGCCGCAACAACCGGATTAACCGCAGCCGCAGTATAAGCAGCCGCAATATCACAAGAAGCAAAAACAGGATGCACCGCAGAAGCAAAATCAGCAGACACAACAGCCGATTCCACAgcagcagcacaatc                                                     >  8:911646/1‑135 (MQ=19)
                                                                                         gTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGATGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGGTACACAGCAGACGCAGTATcc                                                    >  1:791018/1‑137 (MQ=37)
                                                                                            tCAGCCGCAGCAAGAACCGGATGCACCGCAGCCGCAGGATCAGCAGCATCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATcagca                                                 <  2:1068735/138‑1 (MQ=21)
                                                                                            ccagcagcagCAACATCCGCTGGAACCTCATCAGCAGTCTCAGCAGGATCAATATCAACATCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCAAAACAGCCGGTTACACAGCAGCAGCAGTATCagcagc                                               <  4:10423/139‑1 (MQ=18)
                                                                                              agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCg                                             >  7:244702/1‑140 (MQ=255)
                                                                                                cagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                              <  6:896960/121‑1 (MQ=255)
                                                                                                cagcCGCAACAACCGGATGCACCGCAGCCGCAGTATCTGCAGCAGCAATATCAACATCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCg                                             <  1:715548/138‑1 (MQ=25)
                                                                                                  gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCacc                                         >  3:1161450/1‑138 (MQ=255)
                                                                                                   cagCAAGAACCGGACTCACCGCCGCCGCATGCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                        <  7:1096965/138‑1 (MQ=18)
                                                                                                    cGCAACAACCGGTTGCACCGCCGCCGCAGTATCCGCAGCCGCAATATCACCAGCCGCAACAACCGGTGGCACCGCCGCAGCAATATCAGCATCCGCAACAGCCGGTTACCCAGAAGCCGCAGGATCCGCAGCCGCCACAg                                       >  6:852062/1‑140 (MQ=25)
                                                                                                    cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAAAAGCCGCAACAACCGGTTTCACCGCCGCAGCAATATCAGAAGCCACAACAGCCGGTTCCACAGCAGCCGCAGTAGCCGCAGCCGCAAc                                         >  2:755698/1‑138 (MQ=37)
                                                                                                     gCAACATCCGCTCGCAGCGCAGCCGCAGTCTCAGCAGCAGCAATATCAACAGCCTCAACAACAGGTTGCACCTCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                      <  4:361745/140‑1 (MQ=21)
                                                                                                      caagcacCGCTAGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                       <  2:378698/133‑1 (MQ=18)
                                                                                                      caacaaCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAAGATCACGAGCAGCAACAACCGGTTGCCCCGCAGGAGCATTATCCGCCGCCCCAACAGCCGGATCCACAGCAGCGGCAGAAGCCGCAGCCGCAagag                                       >  2:718911/1‑138 (MQ=19)
                                                                                                      caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                       >  1:311841/1‑138 (MQ=255)
                                                                                                      caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                     >  4:616740/1‑140 (MQ=255)
                                                                                                      caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCCCCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCCGCCGCAACAg                                       >  1:593535/1‑138 (MQ=255)
                                                                                                         catccGGTTGCACCGCAGCCGCATCATCAGCATCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGt                                  <  3:1162085/137‑1 (MQ=255)
                                                                                                         catccGGATGCACCGCAGCCTCATGATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACATCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCg                                    <  2:164736/135‑1 (MQ=21)
                                                                                                                aagCACCGCAGCCGCAGACTCCGGAGCAGCATTATCACCAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                           <  8:1042911/138‑1 (MQ=18)
                                                                                                                 agCACCGCAGCCTAAGCATCAGCAGCAGCAATATCATCAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                          <  7:894585/139‑1 (MQ=21)
                                                                                                                  gCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                           >  1:106019/1‑138 (MQ=255)
                                                                                                                   cACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                          >  1:1205546/1‑138 (MQ=255)
                                                                                                                     ccgcagccgcagCAGCAGCAGCCGCAATATCACCAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                      <  8:561046/140‑1 (MQ=255)
                                                                                                                        cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                   >  7:518087/1‑140 (MQ=255)
                                                                                                                          gcctcagGATCAGCAGCCGCAATATCAACAGCTGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                 <  6:489547/140‑1 (MQ=255)
                                                                                                                          gccgctcTATAAGCATCAGCAATATCATCAGGCGCAACAACCGGTTGCACCGCAGCAGCATTATCTGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                 <  3:1174205/140‑1 (MQ=18)
                                                                                                                          gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                   >  1:220945/1‑138 (MQ=255)
                                                                                                                          gccgcagGCTCAGCAGCCGCAATATCATCAGCCTCAACAACCGCATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                 <  4:442754/140‑1 (MQ=21)
                                                                                                                              cagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGGTACACAGCAGCCGCAGTATCCGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc             >  3:1220080/1‑140 (MQ=255)
                                                                                                                               attctCAGCAGCCGCAATATCACCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc            <  8:794514/136‑1 (MQ=255)
                                                                                                                               agtcgcagcagCCGTAATCTCCTCAGCCGCAACAACCGCTTGCAACTCAGCATCATTATCAGCAGCCACAACAGCCGGTTACACAGCCGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGCCGCAGGATAcc            <  6:60303/135‑1 (MQ=18)
                                                                                                                               agcctCAGCAGCCGCAATATCATCAGCCGCACCAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc            <  8:345110/136‑1 (MQ=255)
                                                                                                                                 tctCAGCAGGCGCAAGATCATCAGCCGCAACACCCGGTTGCACAGCAGCAGCAATTTCAGCAGCCTCAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCt          <  4:674633/138‑1 (MQ=18)
                                                                                                                                 caTCCGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc            <  2:401402/137‑1 (MQ=255)
                                                                                                                                        agcagcAATATCACCAGCCTCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTc     <  1:67485/138‑1 (MQ=255)
                                                                                                                                        agcagcAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagc                                                                 >  5:528059/1‑78 (MQ=255)
                                                                                                                                        agcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagc                                                                 <  6:528059/78‑1 (MQ=255)
                                                                                                                                         gcCTCAATATCAACAGCCGCAACCACCGGTTGCACCGCAGCATCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc  <  7:863837/140‑1 (MQ=255)
                                                                                                                                         gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc  >  6:238818/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATC  >  NZ_CP022686/4440185‑4440461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A