Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,324 C→A 11.4% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3240CA11.4% 304.4 / 3.7 170P794Q (CCG→CAG) ftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (64/77);  new base A (10/17);  total (76/94)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440186‑4440463
                                                                                                                                          |                                                                                                                                           
ttgttgAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTGGCACCGCAGCCGCAGTATAAGCAGCCTCAAAAGCCGCTTGAACGTAAGCCGCAGTATCCGCAGCCg                                                                                                                                            >  1:859860/1‑140 (MQ=18)
ttgttgAACCTGTACAGCAGCCGCAACAACCGCTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGCTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCg                                                                                                                                            <  8:968632/140‑1 (MQ=255)
  gttgATCCTGTACAGCATCCGCAACAACCGGTAGCTCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACAGGTTGCACCGCAGCCGCAGTATCAGCAGCCg                                                                                                                                            <  4:413930/138‑1 (MQ=255)
  gttgAACCTGTACAGCAGCCTCACCAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCa                                                                                                                                          <  5:449900/140‑1 (MQ=255)
    tgAAGCTGTACAGCAGGCGCATCAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAat                                                                                                                                        <  6:999607/140‑1 (MQ=255)
     gAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAAACGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAata                                                                                                                                       >  7:8826/1‑140 (MQ=255)
            tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTGGCACCGCAGCCGCAGTATCAGCAGCCGCATCACCCGGTTCAACCGCAGCCGCAGGCTCCGCAGCCGCATAATcagcag                                                                                                                                >  5:783115/1‑140 (MQ=17)
             actgcaggCGAAAGATCCGGTATCACAGATGCAGGAATATCAGCAGCCGCTACTAAGGGTAGATCGGGAGCCGCAGTATCTGCAGCCGCAACTACCGGTTGAACCGCATCCGCTGTATCAGCAGCCGCAATATCAACAga                                                                                                                               <  6:1160027/137‑2 (MQ=255)
                cagacagCAACAACCGGTAGCACCGCATCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGc                                                                                                                            <  8:698891/134‑1 (MQ=255)
                 cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCTGCAGCAGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAAAATCAACAGCCGca                                                                                                                           >  8:35292/1‑140 (MQ=21)
                 cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGGATCCGCAGCCGCAACAACCGGTTGCACCTCAGCCGCAGAGTCCGCCGCCGCAAGATCAGCAGCCGcc                                                                                                                           >  5:1215461/1‑139 (MQ=17)
                 cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCg                                                                                                                             <  3:183834/138‑1 (MQ=255)
                   gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGAACCGCAGACGCAGTATCTGCAGCCGCAACAACCGGTTGCTCAGCAGCCGCAGTATCAGCAGCAGCAATATCAGCCGCCGcaac                                                                                                                         >  1:1296318/1‑140 (MQ=21)
                               ggTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCTCCGCAGCAGCAGTCTCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCAc                                                                                                             <  8:474315/140‑1 (MQ=255)
                                   gctccGCAGCAGCAATATCAGCAGCCGCAACAACCTGTAGGACCGCATCAGCAGTCTCAGCAGGCGCAACAACCTGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                         <  5:1059439/137‑1 (MQ=21)
                                      ccGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACAACCGGGTGACCCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGCACCATCCGGTGGCGCCgcag                                                                                                        >  4:8719/1‑138 (MQ=18)
                                       cGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCCACATCCGGTTGCACCGCAGCCGCAGTATCCGCTGCGGCTATATCAGCAGCCGCAACATCCGGTGGCCCCgcagcag                                                                                                     >  8:1126157/1‑140 (MQ=21)
                                       cGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCTCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATAAGCAGCATCAGAACACGGTTGCACAgcagaag                                                                                                     >  7:289535/1‑140 (MQ=17)
                                         cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCCGCCGCAGTATCAGCAGCAGCAATATCCAAAGCCGCAACAACCGGTTGCCCCgcagcagca                                                                                                   >  5:294916/1‑140 (MQ=21)
                                         cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATCTCAACAGCCGCAACAACCGGTAGCACCgcagcagca                                                                                                   >  6:586918/1‑140 (MQ=255)
                                         cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCATCAGCCGCAACATCCGGTGGCACCgcagctgca                                                                                                   >  8:193153/1‑140 (MQ=21)
                                                        cagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCTACATCCGGTTGCACCGCAGCCGCAGAATCCGCACCCGCAATAACAACAGCCGCCTCACCCGGATGCACCTCAGAAGCAAAATCAGCGGccc                                                                                      >  3:1251023/1‑137 (MQ=18)
                                                         agcCGCAACATGAGGTAGCAGCGCAGACGCATGAGCAGCTGCAGCAACTTCCGCTTGCACCGCAGCAGCAGTATCAGGAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  5:334055/140‑1 (MQ=21)
                                                         agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   >  7:1120199/1‑140 (MQ=255)
                                                           ccGCAACATCCGGTAGCTCCGCAGACGCATTATCAGCAGCATCAACAACCTGTTGCACCGCAGCCGCAGGATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  4:460954/138‑1 (MQ=25)
                                                           ccGCAACAACAGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  3:437314/138‑1 (MQ=255)
                                                           cagCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCTGTTGCACCGCAGCCGCAGTATCAGCAGGCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  7:1071955/138‑1 (MQ=255)
                                                           cagCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  5:1273742/138‑1 (MQ=255)
                                                            cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCTCCTCAGCCGCAGTATCCGCAGCCGCAAAATCATCAGACGCAACATCCGGTGTCACCTCAGCAGCAATAACAGCACCCacgacat                                                                                >  2:864531/1‑139 (MQ=18)
                                                             gCAACAACCGGAAGCACCGCAGCCGCAGTAGCAGCAGCCTCAACTACCGGTTGCACAGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                               <  2:560575/140‑1 (MQ=37)
                                                              caacaaCCGGTAGCACCGCAGCCGCAGTATCCGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCAGCATAATCAGCAGCCGCCACAACCGGATCACCCGAAGCAGCAAACTCCGCAGCCACAACAg                                                                                >  4:971028/1‑138 (MQ=17)
                                                               atcaacCGGAAGCACCGCAGCCGCAGGCTCAGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCg                                                                             <  7:7383/138‑1 (MQ=37)
                                                                     cGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGATGCACCGCAGCAGCAATATCAGCCGCCACAACAGCCGGGTaca                                                                       >  7:302524/1‑140 (MQ=255)
                                                                         aGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCATCAGCCACAACAAGCGGTTGCACCGCAGCAGCAATCTAAGCAGACACAACAGACGGTTACGcagg                                                                   >  8:671485/1‑139 (MQ=21)
                                                                           cacaGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagc                                                                   >  4:894674/1‑138 (MQ=255)
                                                                            cccgcagcagaagTATCAGCAGCCGCAAGATCAGGATGATCCGCAGCCGCAGTCTCTGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTATACagcagc                                                                <  6:1004649/139‑1 (MQ=25)
                                                                            aCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagca                                                                  <  3:66676/138‑1 (MQ=255)
                                                                            aCCGCAGCCGCAGGATCAGCAGCCGCAACAACCGGTGGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagca                                                                  <  3:1059039/138‑1 (MQ=255)
                                                                             cctctgccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGACGCAGTATCAGCAGGAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                               <  6:1252502/135‑1 (MQ=37)
                                                                             ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcagcag                                                                 <  4:1104356/138‑1 (MQ=255)
                                                                              cgcatacgcagTATCAGCAGCCGCAACATCCGTCTGCACCGCAGACTAAGTATCAGCAGCCGCAATATCAACAGGCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  3:588748/132‑1 (MQ=37)
                                                                              cgcagccgcagTCTCAGCAGCCGCACCCACCGGACGCACCGCAGCCGCAGTAACAGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                              <  5:1085010/140‑1 (MQ=37)
                                                                              cgcagccgcagTCTCAGCAGCCGCAACATCAGGTTGCACCGCAGACGCAGTATCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  3:130453/138‑1 (MQ=38)
                                                                              cgcagccgcagGATCAGCAGGAGCATGTACCTGATGCACCGCAGCCGCAGTCTCAGCAGCATCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                              <  2:522622/140‑1 (MQ=25)
                                                                                cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                            >  2:1158528/1‑140 (MQ=255)
                                                                                cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATAAGCAGCCGCAAAAGCATCATCAGCAACAGCCGGATGACCCTCAGCAGCAATATCCGAAGCCGCAACAGCCTGTGACGCagcagcagcc                                                            >  8:1167136/1‑139 (MQ=16)
                                                                                  gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAAGATCTGCAGCCGCCTCATCCGGATGCACCGCAGCAGCAATATAAGACGCCTCAACAGCAGGAGACGCAGCAGCCggt                                                            >  4:838855/1‑136 (MQ=18)
                                                                                      cagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCACCGGCCGCAACAACCGGTTGCCCCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGGTACACAGCAGCCGCAGGATca                                                      >  1:544514/1‑140 (MQ=37)
                                                                                        gTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATAAGCAGCAGCAACAACCGGTAGCACCGCAGCAGCAAAATCCGCAGCCACAACAGCCGGTTCACCAGCAGCCGCAGTATccgc                                                    >  7:94351/1‑140 (MQ=25)
                                                                                           tCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCTCCAGCCGCAACAACCGGTTGCACCGCAGCAGCATTATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGGATCagccgc                                                 >  1:183220/1‑140 (MQ=25)
                                                                                           tCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGACGCAATATCACCAGACGCAACAACCGGATTCAACGCAGCAGCAAAATCAGCAGCAACAACAGCCGGTTACACAGCAGCCGCAGCATCcgccgc                                                 >  5:1110757/1‑140 (MQ=25)
                                                                                              ccagcagCAACCACCTGATGCTCCGCACCGGCACTATCAGCAGCAGCAATATCATCAGCCTCCACACCCGGATGCAACGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTCCACAGCAGCCGCAGTATCAGCAGcc                                                <  4:606719/137‑1 (MQ=17)
                                                                                                 gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGAACCGCAGCCGCATGATCAGCAGCCACATCAGCCGGTTAGCCAGCAGGGCCAGTAACCTCCGCCGCaaa                                           >  6:215082/1‑139 (MQ=21)
                                                                                                 gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                           >  5:1242225/1‑140 (MQ=255)
                                                                                                 gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCCCCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                           >  7:453196/1‑140 (MQ=255)
                                                                                                 gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTACACAGCAGCCGCATAATCAGCAGCCGCAAc                                           >  5:534996/1‑140 (MQ=255)
                                                                                                 gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                           >  7:350546/1‑140 (MQ=255)
                                                                                                  ccGCAAGATCCGGTTGCACCGCAGCCGCAGCATCAGCAGCAGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  7:101585/140‑1 (MQ=21)
                                                                                                  ccGCAAAAACCGGTTGCACCTCAGCCGCAGGATCAGCAGCAGCAAAATCAAAATCCGCAAAAACCGGTTGCACCGCATCAGCAATATCAGCAGCAACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  7:671485/140‑1 (MQ=21)
                                                                                                  cagCAACAACAGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCATCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  1:1053296/138‑1 (MQ=255)
                                                                                                   cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAAGATCAGCAGCCGCCACAGCCGGTTACACAGCAGCCGCAGTAACAGCAGCCGCata                                           >  3:770989/1‑136 (MQ=38)
                                                                                                   cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCCGCAGCCGCAACAg                                         >  5:559985/1‑140 (MQ=255)
                                                                                                    gctacatcCTGTTGAACCTCAGCCGCAGTCTCATCATCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTCCACAGCAGCCGCAGTATCAGCAGCCGCAACAGc                                        <  2:1178447/137‑1 (MQ=18)
                                                                                                     catgaacCTGATGCACCGCAGCCGCAGCCTCTGCAGCAGCAATATCATCAGCCGCAACAACCGTTTGCACCGCAGCAGCATTATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGcc                                       <  8:338294/136‑1 (MQ=255)
                                                                                                     catcaacCGGTTGCACCGCAGACGCAGTCTCTGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         <  3:1069319/135‑1 (MQ=21)
                                                                                                     caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                             <  3:1126259/86‑1 (MQ=255)
                                                                                                     caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGGTACCCAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         >  4:1139365/1‑138 (MQ=255)
                                                                                                     caacaaCCGGAAGCACCGCAGCCGCAGGCTCATCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                       <  1:188270/140‑1 (MQ=21)
                                                                                                                tGCGCCGCAGCCGCAGTAGCAGCAGCCGCAATATCAACCGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGCTTACACAGCAGCCGCAGTATCAGCAGACGCACCAGCCGGTTGtccaac                            >  6:16705/1‑135 (MQ=21)
                                                                                                                tGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  6:774381/104‑1 (MQ=255)
                                                                                                                tGCACCGCAGCCGCAGAATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTAGCACCCCAGCAGCAATATCAGCAGACACAACAGCCGGGTACCCAGCAGCCGCAGTATCAGCACCCGCATAAGCCGTGTGTACaac                            >  2:1018804/1‑140 (MQ=18)
                                                                                                                 gCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                             >  4:649283/1‑138 (MQ=255)
                                                                                                                  cccctcagccgcagCCTCAGCATCACCCTTATCATCACCCGCAACAACCGGATGCACCGCAGCAGCAATATCACCAGCCACAACACCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                          <  8:373918/135‑1 (MQ=17)
                                                                                                                  cACCGCCGCCGCAGTCTCAGCAGCCGCATGATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACACCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                          <  8:1173504/140‑1 (MQ=21)
                                                                                                                  cACCGCAGCCGAAGTCTCAGCAGCCTCAATATCCACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                            <  4:948302/138‑1 (MQ=255)
                                                                                                                   cccgcagccgcagTCTCAGCAGCCGCACTAACACCACCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCACCAGCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACcaca                           <  4:261187/137‑1 (MQ=21)
                                                                                                                   aCCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGc                         >  8:282586/1‑140 (MQ=255)
                                                                                                                    ccgcagccgcagTATCAGCAGCCTCATTATCAACAGCCGCAACAACCGTTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                        <  2:758511/140‑1 (MQ=255)
                                                                                                                    ccgcagccgcagTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCCCAGcc                        >  7:566502/1‑140 (MQ=255)
                                                                                                                    ccgcagccgcagTATCAGCAGCCGCAAAATCAACAGCCGCAACAACCGCTTGCACCCCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCTCAGTATCCGCAGCCGCACCAGACGGATGAACCACAGcc                        >  1:162861/1‑140 (MQ=25)
                                                                                                                     cgccgtcgccgTCTCAGCTGGCGCAATTTCAACAGCCGCATCCTCCGGCCGCACCGCAGCAGCAATATCAGCAGCCCCAACACCCGGTTTCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGc                         <  4:572597/132‑1 (MQ=14)
                                                                                                                     cgcagcagcatGCTCAGCAGCCTCAAGTTCAACAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                       <  1:677832/140‑1 (MQ=18)
                                                                                                                     cgcagcagcagGATCAGCAGCAGCATTATCACCACCCGCATCATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                       <  1:677828/140‑1 (MQ=18)
                                                                                                                      gcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGTACCACAGCCGc                      >  5:824680/1‑140 (MQ=255)
                                                                                                                      gcagcagcagTCTCTGCAGCCGCAATATCAACAGCCGCAAATACCGGTTGCACCGCAGCAGCAATCTAAGCAGCCACAACAGCCGGATACACAGCAGCCGCAGTATCAGCAGCCTCAACAGCCGGTTGTACCACAGCCGc                      <  7:155521/140‑1 (MQ=21)
                                                                                                                       cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGAACCGCAGCCGCAGTATCAGCAGCCACAACAGCCGGTTAAGCAGCATCCGCAGAGTCAGCCGCCGCAACAGCCGGTTGGACCACAGCCgaa                     >  1:316763/1‑138 (MQ=21)
                                                                                                                       cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTGCCACAGCCGCa                     >  6:268236/1‑140 (MQ=255)
                                                                                                                         gccgcagTATCAGCAGCCGCAATATCAACAGCCTCAACATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     <  3:652815/138‑1 (MQ=255)
                                                                                                                         gccgcagGATTCTCAGCCGCATTATCACCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   <  8:812364/140‑1 (MQ=18)
                                                                                                                         ccagcagTATCAGCAGCCTCAAGATCATCAGCCGCAACAACCGGTTGCACAGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   <  2:1342669/137‑1 (MQ=18)
                                                                                                                              agTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATa                <  4:209379/138‑1 (MQ=255)
                                                                                                                                   ctgcagcCTCAAGATCACCTGCCGCACCAACCGGAGGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGAAACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTg           <  4:1005448/136‑1 (MQ=18)
                                                                                                                                   aagcagcCGCAATATCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt         <  6:828028/139‑1 (MQ=255)
                                                                                                                                      cagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa      >  2:287795/1‑140 (MQ=255)
                                                                                                                                        gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  7:868426/1‑140 (MQ=255)
                                                                                                                                          cGCAATATCACCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGTTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  <  1:1027849/140‑1 (MQ=255)
                                                                                                                                          cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACGCTGCTTCATCCg  >  8:1045792/1‑140 (MQ=255)
                                                                                                                                          cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCCGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  5:712980/1‑140 (MQ=255)
                                                                                                                                          cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTCCCACAGCCGCAGGATACCCTGCTTCATc    >  4:1276309/1‑138 (MQ=255)
                                                                                                                                          cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGAATCAGCAGCCGCAACAGCCGGTTGTACCACAGACTCAGGATACCCTGCTTCATc    >  3:908433/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440186‑4440463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A