Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F36 I0 R1 179 8076.5 8499890 98.3% 8355391 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,324 C→A 14.3% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3240CA14.3% 218.3 / 2.1 119P794Q (CCG→CAG) ftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (39/53);  new base A (13/10);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440188‑4440463
                                                                                                                                        |                                                                                                                                           
gttgAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCa                                                                                                                                          <  3:620578/140‑1 (MQ=255)
     acatGTACAGCAGCCGCAACAACCGGTAGCACCGCATCAGCAATCTCAGCAGCCGCAACAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCTCAGCCGCAGTATCAGCAGCCGCAATATc                                                                                                                                     <  3:769353/137‑1 (MQ=21)
         gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAACAGCCGGTTGAACCTCAGCCGCAGTATCCGCAGCAGCAACATCAACa                                                                                                                                 >  2:357549/1‑140 (MQ=17)
         gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCAATAgcagca                                                                                                                                 >  1:224634/1‑140 (MQ=255)
          tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCACAGCCGCAGTATCAGCAGCGGCAAAATccgcag                                                                                                                                >  4:470021/1‑135 (MQ=17)
          tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGATGAACCGCAGCCGCAGTATCAGCAGCAGCATAATCagaag                                                                                                                                >  3:766393/1‑136 (MQ=18)
           aCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                               <  4:167059/140‑1 (MQ=255)
             atcagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCg                                                                                                                             <  1:176125/138‑1 (MQ=255)
               cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCTCAACAACCGGTACCACCGCAGCCTCAGTATCAGCAGCCGCAACAACAGGTTGAACCACAGCCGCAGGAAAAGCAGCATCAAAATcagcagatgca                                                                                                                           >  4:159296/1‑135 (MQ=14)
               cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTTCCCCGCAGCCGCAGTATCAGCAGCCGCAAAATCAgcagcagca                                                                                                                           >  2:203689/1‑140 (MQ=21)
               cagcCGCAACAACCGGTAGAACCGCAGCAGCAATATCAGCAGCCTCAACATCCGGTAGAACAGCAGCCGCAGTATCAGCAGCCTCAAAACCCGCTTCAGCCCCAGCCGCATGATCAGCAGCAGCAAAATCAgcagcagca                                                                                                                           >  4:296121/1‑140 (MQ=9)
                agcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                          <  3:39176/140‑1 (MQ=255)
                agcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                          <  2:175201/140‑1 (MQ=255)
                 gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCCCAGCCCCAGAATCAGCAGCCGCAAAATCACCAGCCGcaaa                                                                                                                         >  3:719983/1‑139 (MQ=18)
                 gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGAACCTCAGCCGCAGGATCATCAGCGGCAAAATCAGCAGCAGcaaa                                                                                                                         >  6:155488/1‑139 (MQ=17)
                 gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGGAGACGCAGCAGCAAACTCAGGAGCCGca                                                                                                                           >  8:778368/1‑138 (MQ=14)
                 gcCGCAACAACCGGTAGCACCGCAGCAGAAATATCAGCAGCCGCAACACCCGGAAGCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGGAACCGCAGCGTCAACATCACCAgcagcata                                                                                                                         >  2:150912/1‑138 (MQ=14)
                  ccGCAACAACCGGTATCACCGCAGCAGCAATATCAGCAGCCTCAACAAACGGTAGCACCTCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                        <  5:973662/140‑1 (MQ=21)
                  ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCTGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                        <  5:866180/140‑1 (MQ=255)
                  ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                        <  3:1023104/140‑1 (MQ=255)
                    gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGAACCGCAGCCGCAGTATCAGCAGCAGCAATATCATCAGCCGCcacaac                                                                                                                      >  3:697094/1‑140 (MQ=255)
                        ccacctctAGCTCCGCAGCAGCAAGATCAGCAGCCGCATCAACCTGTATCACCGCAGCAGAATGATCAGCAGCCGGAACAACCTCATGCACCGCAGCCGCCGGATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGt                                                                                                                  <  4:69243/133‑1 (MQ=255)
                           ccGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGtt                                                                                                                 <  7:20563/138‑1 (MQ=255)
                               aaGCACCGCAGCAGCAATATCACCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGGATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCg                                                                                                           <  4:368759/139‑1 (MQ=255)
                                aGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCATCCGCAAAACCCGGTTGCACAGCACCAGCAGTATCAGCAGCCGGAATATCACGAGCCGGAACAACCGGTTGCGcc                                                                                                            >  7:89287/1‑138 (MQ=18)
                                   tgcGCAGCAGCATGATCAGCAGCCGCATCAACCGGAAGCACCGCAGCAGCAGGATCAGCAGCAGCATCAACCTGATGCACCGCAGCCTCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                       <  2:914973/138‑1 (MQ=21)
                                      gcagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagc                                                                                                    >  1:928112/1‑140 (MQ=255)
                                       cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGGATCCGCCTCAGCAATATCCACAGCAGCGACAGCCCGATGGACGgcagcagca                                                                                                   >  6:56647/1‑140 (MQ=18)
                                       cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcagca                                                                                                   <  1:375592/140‑1 (MQ=255)
                                                aatCAGCAGCCGCAACACCCGGTAGCTCCGCATCAGCTGTATCAGCAGGAGCAACTACAGGTTGCACCGCAGCCGGAGTATCAGCAGCCGCTTTATCAACAGCCGCAACTACCTGTTGCACCGCAGCAGCAATATcagca                                                                                          <  6:113977/139‑1 (MQ=255)
                                                  tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCTGCAGCAATATCagcagc                                                                                        <  4:724495/140‑1 (MQ=255)
                                                  tCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCAGCAATATCAACAGCCGCCACAACCGGTTGCACATCAGCAGCATTATCggcagc                                                                                        >  6:975992/1‑140 (MQ=18)
                                                    agcagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGACGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCca                                                                                      <  3:409928/140‑1 (MQ=255)
                                                         ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCTGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  1:518389/140‑1 (MQ=255)
                                                         ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCAGCAAAATCAACAGCCGCCACAGCCGGTTGCACCGCAGCAGCAATATCAGCAGCCccaaca                                                                                 >  3:642983/1‑140 (MQ=25)
                                                         ccGCAACAACAGGTAGCACCGCAGACGCATTATCAGCAGCCTCATCAACAGGTTTCACCTCAGCAGCAGTATCAGCAGCATCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  4:380204/140‑1 (MQ=25)
                                                           ggatcacccGGTATCTCAGCAGCAGCATGATCAGCAGCCGCAACAACCGCTTGCACCGCAGCCTCAGTATCAGGAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGc                                                                               <  6:270695/133‑1 (MQ=25)
                                                           gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCCCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  7:211988/138‑1 (MQ=255)
                                                            caacaaCCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAg                                                                                <  7:883360/138‑1 (MQ=255)
                                                                      tAGGACCGCAGCCTCAGTATCAGCAGCCGCATCAACCTTTTTCTACGCAGCCGCAGTATCTTCTTCCGCAATATAAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                      <  7:28688/138‑1 (MQ=21)
                                                                       aGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCAACAACAGCCGGTTacccc                                                                     >  7:20760/1‑135 (MQ=255)
                                                                         cACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAGCAGCCGCAACATCCGGATGCACCGCAGAAGCAATATCAGCAGCCGCAACAGCCGGATACAcatcag                                                                 >  1:111867/1‑140 (MQ=25)
                                                                          aacgcatccgcagTCGCTGCAGCAGCATCGACCTCATGCACCGCTGACTCAGTCTAAGCAGCCTCAATTACATCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  6:56246/138‑1 (MQ=12)
                                                                          aCCGCATCCACAGGATCAGCAGCCGCCATAACCCGATTCTGCACCGACATAGTAAAATCAGCCTCAATAACATCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  1:1049994/140‑1 (MQ=9)
                                                                          aCCGCATCAGCATTATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGGATCAGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  4:83702/140‑1 (MQ=37)
                                                                          aCCGCAGACTCAGTATCAGCAGCCGCATCAACCGCTTGCACAGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  6:990605/140‑1 (MQ=37)
                                                                          aCCGCAGACGCATGATCTGCAGCAGCATCAACCTGATGCACCGCAGACGCAGTATAAGCAGCCTCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  4:497552/140‑1 (MQ=25)
                                                                           ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCa                                                                                                                                          <  1:917182/65‑1 (MQ=255)
                                                                              cagccgcagTATCAGCAGCCTCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGACGCAACAACCGGTTGCACCGCAGCAGCAATATCCGCAGCCACAACAGCCGGTTACTCATCAGCCGCa                                                            >  5:1018506/1‑140 (MQ=37)
                                                                              cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCATCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACCCAGCAGCCGcc                                                            >  4:313933/1‑139 (MQ=255)
                                                                              cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCATCATCCGGTGGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACACagcagcagcc                                                            >  2:842633/1‑139 (MQ=37)
                                                                              cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                            >  6:990678/1‑140 (MQ=255)
                                                                              cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACATCAGCCGCa                                                            >  6:847777/1‑140 (MQ=255)
                                                                                gccgcagTATCAGAAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTTCACCGCAGCAGCAAAATCAGCATCCACAACAGCCGGATACACAGCAGCCGCa                                                            >  7:786359/1‑138 (MQ=38)
                                                                                    cagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATca                                                      <  1:943567/140‑1 (MQ=255)
                                                                                      ctctCAGCAGCCGCAACAACCGCACGCACCGCAGCCGCAGGCTAAGCAGCAGCATTATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATcagc                                                    <  4:58398/137‑1 (MQ=21)
                                                                                              agcCGGAATTACCTGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAGCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCaa                                            <  3:579463/140‑1 (MQ=18)
                                                                                              agcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCAGGAGCAGCAACATCCGGTTGCCCCGCAGCAGCATTATCAGCAGCCGCCACAGCCGGTTAGACAGCAGCCGCAGTATCCGCCGCCGCaa                                            >  4:859494/1‑140 (MQ=25)
                                                                                               gcCGCAACAACCGGTTTCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACACACGGTTGCACCGCAGAAGCATGATCAGCAGCCTCAACAGCCGGTTACCCAGCAGCAGCAGTTTCAGCCGCCGCAAc                                           >  6:392097/1‑140 (MQ=37)
                                                                                               gcCGCAACAACCGGCTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCCCCGCAGCAGCATGATCAGCCGCCGCGCCAGCCGGGTCCCCAGCTGCCGCACTATCAGCAGCCGCatc                                           >  2:139297/1‑138 (MQ=25)
                                                                                                ccGCAACTACCGGCTGCACCGCAGCCTCAGTATCATCAGCCGCAAGATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  1:701813/140‑1 (MQ=21)
                                                                                                ccGCAACAACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  5:502338/140‑1 (MQ=255)
                                                                                                cagCATCAACCTGTTGCTCCGCATCAGAAGTATCAGCAGCAGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  2:421200/138‑1 (MQ=18)
                                                                                                 cGCAAGAACCTGTAGCACCGCAGCAGCAGTCTCTGCAGCCGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         <  1:427968/140‑1 (MQ=21)
                                                                                                 agCAACAACAGGATTCACCGCAGCCGCAGTATCAGCAGCATCAATATCAACAGCCTCAACAACCGCTTGCACCGCAGCAGCATTATCAGCAGCCACAACAGACGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         <  1:694572/139‑1 (MQ=21)
                                                                                                   caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCCGCAGCAATATCAGCCGCCCCAACAGCCGGGTACACCGCAGCCGCAGTATCCGCAGCCGCAAccg                                         >  8:896182/1‑136 (MQ=38)
                                                                                                   caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                       <  2:2489/140‑1 (MQ=255)
                                                                                                             ttGCCCCGCAGACGACGGAACAGCAGCACCAAGATCAACACCCGCAACACCCGGATGCACCGCAGCATCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCTCAACAGCCGGTTGTACca                             <  5:624918/140‑1 (MQ=18)
                                                                                                             ttGCACCGCAGCCTCAGGAGCAGCAGCAGCAATTTCAACAGCCGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAc                               <  7:94586/138‑1 (MQ=21)
                                                                                                             ttGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                             >  5:825835/1‑140 (MQ=255)
                                                                                                              tGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                            <  4:888504/140‑1 (MQ=255)
                                                                                                              agCACCGCAGCCGCAGTCTCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCCGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                            <  4:297172/139‑1 (MQ=255)
                                                                                                                cACCCCAGCCGCAGCATCAGCAGCCGCACTAACCACAGCCGCAACATCCTCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                          <  1:588573/140‑1 (MQ=21)
                                                                                                                  ccgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                        <  5:990678/140‑1 (MQ=255)
                                                                                                                  ccgcagccgcagTATCAGCAGCCGCAATATCAACACCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                          <  8:365432/138‑1 (MQ=255)
                                                                                                                   cgctgccgcagTATAAGCATCAGCATTATCATCAGCCTCAACAACAGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                       <  3:687491/140‑1 (MQ=18)
                                                                                                                    gcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGc                      >  1:412839/1‑140 (MQ=255)
                                                                                                                     cagcctcagTCTCTGCTTCCGCATGATCACCAGCCTCAACAACCGGTTGCACCGCAGCAGCACTATCAGCAGCCACAACAGCCGGTTACACATCATCAGCAGTATCAGCAGCAGCAACATCCGGTTGTACCACAGCCg                       <  7:407108/138‑1 (MQ=25)
                                                                                                                     cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 >  4:987276/1‑80 (MQ=255)
                                                                                                                     cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     >  4:1080941/1‑140 (MQ=255)
                                                                                                                      agccgcagTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCCGCCGCAACAGCCGGTTGTACCACAGCCGc                      >  7:421974/1‑138 (MQ=255)
                                                                                                                       gcggcagTATCCTGAGCCGCAATATCATCAGCCCCATCTCCCGTTTGCACCGCAGCAGCATTATCAGCAGCCTCAACAGCCGGTTACACTGCAGCCGCAGTATCAGCAGCCGCACCAGCCGGTTGTACCACAGCCGCAgg                   <  1:158102/137‑1 (MQ=18)
                                                                                                                            attctCAGCATCCTCAATATCACCATACGCAACAACCGGTATCACCGCAGCAGCAATATCATCAGCCCAACCAGCAGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGCCGCAGGATa                <  7:98635/134‑1 (MQ=17)
                                                                                                                            agTATCAGCAGCCGCAATATCAACAGCCGCAACATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc              <  1:751575/140‑1 (MQ=255)
                                                                                                                                tCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACATCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGc          >  3:446907/1‑140 (MQ=255)
                                                                                                                                  agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt        >  5:992614/1‑140 (MQ=255)
                                                                                                                                  agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt        >  1:530883/1‑140 (MQ=255)
                                                                                                                                    cagcCGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACTACAGCCGGTTACACAGCTGCCGCAGTATCACCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa      <  5:305854/140‑1 (MQ=255)
                                                                                                                                      gcCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCa      >  7:928330/1‑138 (MQ=255)
                                                                                                                                      gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  2:606680/63‑1 (MQ=255)
                                                                                                                                      gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  1:532678/1‑140 (MQ=255)
                                                                                                                                      gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  3:592805/1‑140 (MQ=255)
                                                                                                                                      gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGCCGCAGGATACCCAGCTTAATc    >  6:703389/1‑140 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGACGCAACAGCCGGTTGTACCACAGCCGCAGGATCCCCTGCTTCATCCg  >  6:1043847/1‑140 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACGTCCGGTTGTACCACAGCCGCAGGATACCCTGATACATCCg  >  5:228016/1‑140 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTCCCACAGCCGCAGGATACCCTGCTTCATCCg  >  1:42347/1‑140 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc              >  3:975444/1‑128 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  7:677250/1‑138 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  1:277762/1‑140 (MQ=255)
                                                                                                                                        cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  5:703062/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440188‑4440463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A