Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F2 I0 R1 233 6461.8 9239822 98.3% 9082745 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,324 C→A 11.5% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3240CA11.5% 263.2 / 2.7 146P794Q (CCG→CAG) ftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (63/59);  new base A (11/11);  total (75/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440185‑4440463
                                                                                                                                           |                                                                                                                                           
 ttgttgAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCg                                                                                                                                            >  1:275424/1‑140 (MQ=255)
            gTACAGCAGCCGCAACAACCGGTAGCAACGCAGGAGCAATATCAGCAGCCGCAACAACCGGTAGAACCGATGCCGCAGTATCAGCAGCCGCAACTACCGGTTGCTCCGCAGCCGCAGTATCAGCAGCCGCAATATCAACa                                                                                                                                 <  3:9792/140‑1 (MQ=25)
             tACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCTCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGATCCTCAGCCGCAGTATCAGCAGCAGCATAATCACCAg                                                                                                                                >  3:297572/1‑140 (MQ=18)
              aCAGCAGCCGCAACAACCCGTAGAACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCACTATCAGCAGCAGCAACAACCGCTTGCCACGCAGCCGCAGCATCAGCAGCCGAAAAATCagccgc                                                                                                                               >  2:1036469/1‑135 (MQ=18)
                  cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCCAAGCCGCAGTATCCGCTGCAGCAAAATCAGCAGCCGca                                                                                                                           >  7:710331/1‑140 (MQ=17)
                   agcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                          <  7:73079/140‑1 (MQ=255)
                    gcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGAAGCCGCAGTATCAGCAGCCGCAATATCACAAGCCGcaac                                                                                                                         >  1:445573/1‑140 (MQ=255)
                     ccGCAACAACCGGTAGCACCTCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAGTATCAGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                        <  2:771219/140‑1 (MQ=255)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGcaaca                                                                                                                        >  3:958595/1‑140 (MQ=255)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGAAACCGCAGCCGCAATATCACCAGCCGcaaca                                                                                                                        >  4:348151/1‑140 (MQ=255)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGACGCAGTATCAGCAGCCGCAATATCAACAGACGcaaca                                                                                                                        <  7:112798/140‑1 (MQ=255)
                       gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCAGCAACAACCGGTAGCACCTCATCCGCAGTATCAGCAGCCGCAACAACCGGTTTCACCGCAGCCGCAGTAGCAGCAGACGCAATATCATCAGCCGcaacatc                                                                                                                      >  4:342200/1‑138 (MQ=21)
                         aacaacCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTGGACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGCTGGAGCCGCAGCCGGAGAAACAGCAGCGGCATAATCCGAAGCCGccacatc                                                                                                                      >  6:961373/1‑136 (MQ=9)
                             agctgTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGACGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTg                                                                                                                <  2:469468/136‑1 (MQ=255)
                                ggTAGCACCGCAGGAGCATTATCTGCATCCGCAACAAGCGGTAGCACCGCAGCAGCAGTCTCAGCAGCCGCAACAACCGGTTGGACCGCAGCCGCAGTATCAGCAGCCGCATTATCAACAGCCGCAACAACCGGTTGc                                                                                                               <  5:753200/138‑1 (MQ=18)
                                   aGCTCCGCAGCAGCAATCTCACCAGCCGCAACAACCGGAAGCTCCGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATAAGCAGACGCAATATCAAAAGCCGCACCAACCGGTTGCACCgc                                                                                                          <  2:192934/140‑1 (MQ=18)
                                   aGCACCGCAGCAGCAAGATCAGCAGCCGCAACAACCGGAAGCACCGCATCCGCAGTCTCAGCAGCCTCAACATCCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgc                                                                                                          <  2:161094/140‑1 (MQ=25)
                                      aCCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                       <  8:55917/140‑1 (MQ=255)
                                        cGCAGCAGCAATATCAGCAGCCGCAACAACCTGTAGCACCGCAGACGCAGTATCAGCAGCCGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagcag                                                                                                     <  1:399888/140‑1 (MQ=255)
                                          cagcagcaATATCAGCAGCCGCAACAACCGGTAGCCCCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATAAGCAGGAGCCGCCACAACCGGTAGCACCgcagcagcc                                                                                                   >  4:258993/1‑139 (MQ=18)
                                           gtcagcaATATCTGCAGCCGGATGAATCGGTAGGACCGCAGCAGCAGTCTGAGCAGCCGCATCATCCGGTTGCACCGCAGCCGCAGTATCATGAGGCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCaa                                                                                                  <  8:445398/138‑1 (MQ=21)
                                           agcagcaATCTCAGCAGCCGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCaa                                                                                                  <  1:30975/140‑1 (MQ=255)
                                            gcagcaATATCAGCAGCCGCAACAACCGGTAGCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATGTCAGCTGCCTCCACAAGCGGTGGCGCCgcagctgcc                                                                                                   >  6:567220/1‑137 (MQ=17)
                                              agcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCATAATCAAAAGCCGCAACAACCGGGTGCACCGCAGCAGCaaa                                                                                                 >  5:721529/1‑137 (MQ=21)
                                               gcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCACCCGCCGGTTGCACCGCAGCAGCAATATc                                                                                              >  7:655561/1‑140 (MQ=255)
                                                caATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTGTCAGCAGCAGCAAGATCAGCTGCCGCGACATCCGGTAGGCCCTCAGCGGCAAGATca                                                                                             >  1:501454/1‑140 (MQ=17)
                                                  atatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCACCGGTTCCACCGCAGACGCAGTAACAGCAGCCGCATTATCCACATCCGCCACAACCGGTGTCACCGCAGCATCAATATcagc                                                                                           >  3:1013691/1‑140 (MQ=18)
                                                   tatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagca                                                                                          <  3:182946/140‑1 (MQ=255)
                                                     tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCagcagc                                                                                        <  8:241971/140‑1 (MQ=255)
                                                       agcagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAAAATCAACAGCCGCAACATCCGGATGCACCGCAGCAGCAATATCAGCAGccc                                                                                      >  1:292060/1‑139 (MQ=21)
                                                          agcCGCAACATCCGGTAGCACCGCATCCGCATTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGGCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  1:932435/140‑1 (MQ=255)
                                                          agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCCGCcac                                                                                     >  5:579352/1‑138 (MQ=255)
                                                          agcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACAGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  1:1000775/140‑1 (MQ=255)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  1:1194048/140‑1 (MQ=255)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  5:353717/138‑1 (MQ=255)
                                                            ccGCAACAACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  4:431081/140‑1 (MQ=255)
                                                            ccGCAACAACCGGAAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  3:241694/140‑1 (MQ=255)
                                                             cGCAACAACCGGTAGCACCGCAGCCGCAGGATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGAGCAACAGCCTCAACAGCCGGTTGGCCAGCAGCTGGAATTACAGCAGCGCCAACAg                                                                                >  2:376309/1‑140 (MQ=17)
                                                              gCAACAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  5:939950/138‑1 (MQ=255)
                                                              gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAata                                                                                                                                       <  5:230503/84‑1 (MQ=255)
                                                              gCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAata                                                                                                                                       >  6:230503/1‑84 (MQ=255)
                                                                       ggTAGCACCGCCGCCGCAGTATCAGCAGCCGCAACAAACGGGTGCACCGCAGCCGCAGTATCAGCAGCCTCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTac                                                                        <  5:1079485/138‑1 (MQ=255)
                                                                        gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGAAGCAATATCAGCAGCCACAACAGCCGGTTAcaca                                                                     >  3:52353/1‑140 (MQ=255)
                                                                              ccgcagccgcagTATCAGCAGCAGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                               <  1:720156/140‑1 (MQ=255)
                                                                               cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCTCAGCCGCAGTATCCGCCGAATCAATATCAACATCAGCCACAACCGCTTGCACCGCAGCAGCAATATCATCAGCACCAACAGCCGGTAAAAcagcagaag                                                              >  8:1004375/1‑137 (MQ=21)
                                                                               cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACAAGAAGCAACAACCGGATGCCCCGCAGCAGCATTATCAGCAGCCACAACAGCCGGTTAGACAGCAGccc                                                              >  8:108298/1‑139 (MQ=25)
                                                                               cgaagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCATACGCAAAATCACCATCAGCAACAAACGGATGCCCCTCAGCATCAATATCAGAACACCCAAAAGCCTGATACCCATAAGCCg                                                              >  8:500591/4‑140 (MQ=12)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCCGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCCGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                            >  1:959012/1‑140 (MQ=255)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATAAGCAGCCGCAAGATCAGCAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGACACCACAGCCGGATACTCAGAAgcagca                                                            >  2:152274/1‑140 (MQ=25)
                                                                                  agccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCACAAGCAGCAACAACCGGATCCACCGCAGCAGCAATAACAGAAGCCACAACAGCCGGATACACAGAagcagc                                                             >  5:14073/1‑138 (MQ=21)
                                                                                   gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAAAAGCCGCAACAACCGGTGGCACCGCAGCAGCAATATCCGACGCCCCAACAGCAGTATACTCAGCAGCCGCa                                                            >  5:603362/1‑138 (MQ=25)
                                                                                              agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCACTATAAGCAGCCg                                               >  2:993549/1‑140 (MQ=255)
                                                                                               gcagcCGCAACAACCTGTTGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGc                                              <  1:518047/140‑1 (MQ=255)
                                                                                                 agcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCACCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAAAAACAGAAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCCGCAGcccc                                              >  5:187821/1‑136 (MQ=38)
                                                                                                  gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCAGCCGCAAc                                           >  1:152957/1‑140 (MQ=255)
                                                                                                  gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                           >  7:314503/1‑140 (MQ=255)
                                                                                                   ccGCAACAACCGGATGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACa                                          <  1:797009/140‑1 (MQ=255)
                                                                                                    ctcaagcacCTGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAGATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         <  4:758292/133‑1 (MQ=255)
                                                                                                    cGCATCAACCGGATGCACCGCAGCCGCAGTCTCAGCAGCAGCAATATCACCAGCCGCAACAACCGGCTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         <  1:1026407/140‑1 (MQ=21)
                                                                                                    cGCAACAACCGGTTGCCCCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCACCACCCGGTTGCACCGCAGCAGCAATAACAGCCGCCACCACAGCCGGTTACACAGCAGCCGCCGTAGCAGCAGCCGCACCAg                                         >  4:230319/1‑140 (MQ=37)
                                                                                                    cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCTTCAGCCGCAACAACCGGTTGCACCGCAGCTGCAATATCAGCATCCACAACAGCCGGGTACACAGCAGCCGCAGGATCAGCCGCCGCAACAg                                         >  3:870700/1‑140 (MQ=38)
                                                                                                    cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGGTCCCCAGCAGCCGCAGGCTCCGCCGCCGCAAc                                           >  6:622676/1‑138 (MQ=25)
                                                                                                      caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAg                                         >  5:887169/1‑138 (MQ=255)
                                                                                                      caacaaCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                       >  4:1173190/1‑140 (MQ=255)
                                                                                                        acaacCGGTTGCACCGCCGCCGCAGTATCAGCAGCCGCAATATCAACAGCAGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGTATAAACAGCAGACGCAGGATCAGCAGCCGCAACAGcc                                       >  5:745743/1‑138 (MQ=37)
                                                                                                         ctacCGGAAGCACCGCAGCCGCATCCTCAGCAGCCTCAATATCAACAGCCGCAACAACCGCTTGCACAGCAGCAGCAATATCATCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCg                                      <  6:588929/136‑1 (MQ=21)
                                                                                                         caacAGGATGCACCGCAGACTCAGTATCAGCAGCAGCAATAACAACAGCAGCACCAAACGGTAGCAACGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCAGCAGTATCAGCAGCCGCAACAGCCGGt                                    <  4:1029342/140‑1 (MQ=18)
                                                                                                              ggTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCCACAGCAGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTACACAGCAGCAGCAGTATCAGCCGCCGCAACAGCCGGTTGTAc                               >  4:309242/1‑140 (MQ=25)
                                                                                                               gTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTAcc                              >  3:1125908/1‑140 (MQ=255)
                                                                                                                acgCACCGCAGCTGAAGGCTCTGCAGCCGCATTATCACCAGCCGCAACAACCGGTTGCACCTCAGCAGCAATATCAGCAGACACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACca                             <  4:1079655/138‑1 (MQ=18)
                                                                                                                   cACCGCAGCCGCAGTATCAGCAGCCGCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                          <  1:253299/140‑1 (MQ=255)
                                                                                                                   cACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGACGCAACAGCCGGTTGTTCcac                            >  5:724759/1‑138 (MQ=255)
                                                                                                                    aCCGCAGCCGCATGAAAAGCAGCATCATTATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGc                         <  8:914781/140‑1 (MQ=18)
                                                                                                                     ccgcatcagaagCATCAGCAGCCGCATTATCATCAGCCGCACCATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                        <  8:142548/140‑1 (MQ=18)
                                                                                                                     ccgcagccgcttCATCTTCAGCCTCATTCTCAACAGCCTCATCACCCGGAGGCAAGGCAGCAGCATTATCAGCAGCCGCACCAGCCGGTTACACAGCAGCCTCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAg                          <  6:121908/138‑1 (MQ=12)
                                                                                                                     ccgcagacgcagCATCTGCAGCAGCAATCTCAACAGCAGCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                        <  2:59462/140‑1 (MQ=21)
                                                                                                                      cgcagccgcatTATAAGCAGCAGCAATATCAACAGCCTCAACAACCGGTTGCACCGCAGCCGCAATATCAGCAGCCACACCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCg                       <  4:47365/140‑1 (MQ=21)
                                                                                                                        cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACCACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGcc                     >  2:913292/1‑139 (MQ=255)
                                                                                                                          gccgcagTCTCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     <  6:891804/138‑1 (MQ=255)
                                                                                                                          gccgcagTCTAAGCAGCCGCAATATCAACAGGCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   <  7:597299/140‑1 (MQ=255)
                                                                                                                          gccgcagTATCAGCCGCCGCAATATCAACAGCCGCAACAACCGGATGCTCCGCAGCAGCAATATCAGCAGCCAGCACAGGCGGATACACAGCAGCGGCAGTATCAGCAGCCGCAACAGCCGGTGGTACCACATCCGCacg                   >  8:650127/1‑138 (MQ=21)
                                                                                                                          gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACCCAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGcc                     >  5:243518/1‑137 (MQ=255)
                                                                                                                          gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   >  3:557887/1‑140 (MQ=255)
                                                                                                                           ccgcagGAGCAGCAGCCGCAATATCATCAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGa                  <  1:173823/140‑1 (MQ=255)
                                                                                                                            cgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGAAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGGATCAGCAGCCGCAACAGCCGCTTGTACCACAGCCGCAgg                   >  5:479486/1‑138 (MQ=255)
                                                                                                                               agTAGCAGAAGCAGCATTATCCACATTATCAACAACCGGTGGCACCGCAGCAGCAATCTCAGCAGCCACACCAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAcc              <  4:53210/140‑1 (MQ=18)
                                                                                                                                gTATCAGCATCCGCAATATCAACATCAGCAACAACCGGTGGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc               <  5:334998/138‑1 (MQ=255)
                                                                                                                                   tCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGc          >  3:821457/1‑140 (MQ=255)
                                                                                                                                    gagcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCTCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCTCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt         <  3:706908/139‑1 (MQ=255)
                                                                                                                                       cagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt        >  5:982489/1‑138 (MQ=255)
                                                                                                                                         gcCTCATTATCAACAGCCTCACCAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    <  4:802457/140‑1 (MQ=255)
                                                                                                                                         gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                       >  4:897964/1‑41 (MQ=255)
                                                                                                                                         gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGGTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  2:533229/1‑140 (MQ=255)
                                                                                                                                         gcCCCAATATCAACCGCCGCAACAACCGGTTGCACCGCAGCATCAAAATCACCATCCACAACAGCCGGTTACACAGCAGCAGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  4:93247/1‑140 (MQ=21)
                                                                                                                                         cgcGCAATTTCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTTCACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    <  4:399473/138‑1 (MQ=255)
                                                                                                                                           cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGCTACCCTGCTTCATCCg  >  8:221271/1‑140 (MQ=255)
                                                                                                                                           cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  2:195392/1‑140 (MQ=255)
                                                                                                                                           cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  2:911048/1‑140 (MQ=255)
                                                                                                                                           cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  <  3:1095675/140‑1 (MQ=255)
                                                                                                                                           cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  3:894744/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440185‑4440463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A