Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,440,324 C→A 10.8% P794Q (CCG→CAG)  ftsK → DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,440,3240CA10.8% 234.7 / 4.1 130P794Q (CCG→CAG) ftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (54/55);  new base A (8/11);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440185‑4440463
                                                                                                                                           |                                                                                                                                           
aTTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGcc                                                                                                                                             <  6:124857/140‑1 (MQ=255)
 ttgttgAACCTGTTCAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCg                                                                                                                                            >  1:141588/1‑140 (MQ=255)
   gttgAACCTGTACCGCATCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCa                                                                                                                                          <  3:746652/140‑1 (MQ=255)
   gttgAACCTGGACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCa                                                                                                                                          <  1:660590/140‑1 (MQ=255)
     tgAACCTGTACAGCAGCCGCAACAACCGGTATCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAat                                                                                                                                        <  2:765603/140‑1 (MQ=255)
         ccTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCATCAGCAACAACCGGTAGCAACGCAGCAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCa                                                                                                                                    <  4:125473/140‑1 (MQ=255)
            gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCATTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGCACCGCAGCCGCAGTATCGGCAGCGGCAAGATCagaa                                                                                                                                 >  7:70968/1‑137 (MQ=17)
            gTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGAACCGCAGCCGCAGTATCAGCAGCCGAAAAATCAGCa                                                                                                                                 >  5:395678/1‑140 (MQ=18)
              aCAGCAGCCGCAACAACCGCTAGCAGCGCAGCAGCAATATCTGCAGCCGCAACATCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                               <  4:77638/140‑1 (MQ=255)
              aCAGCAGACGCAACAAGCGGTAGCACCGCAGGAGCAATATCAGCAGCCGCATCATCCGGTAGCACCGCAGGAGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGc                                                                                                                               <  6:2809/140‑1 (MQ=21)
                agcagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCATCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCg                                                                                                                             <  4:707750/140‑1 (MQ=255)
                  cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACACCCGGTTGAACCACAGCCGCAGAATACGCAGACGCATTAGAAACAGcgcca                                                                                                                           >  7:568868/1‑136 (MQ=14)
                  cagcCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACAGGTGTCACCGCAGCCGCAGTATCAGCAGCAGCAAACTCAGCAGCCGca                                                                                                                           >  8:792974/1‑140 (MQ=18)
                   agcCGCAACAACAGGTAGCTCCTCTGCATCAATATCAGCAGCCGCATCATACGTTAGCACCGCAGCAGCAGTATCAGCAGCAGCATCAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaa                                                                                                                          <  8:660017/140‑1 (MQ=18)
                   agacGCAACAACCTGTAGCACCGCAGCAGCAAGATCAGCAGCCGCAACAAACTGTAGCACAGCAGCAGCAGTATCAGCAGCAGCAACAACCGGTTGCACAGCATCCGCAGTATCAGCAGCAGCAATATCAACAGCAGcaa                                                                                                                          <  6:721520/137‑1 (MQ=255)
                     ccGCAACAACCGGTAGCACCGCTGCAGCAATAGCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATAAGCAGCCGCAACAACCGGTTGCACAGCAGCCTCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                        <  2:399614/140‑1 (MQ=25)
                     ccGCAACAACCGGTAGCACCGCAGCAGCACTATCAGCAGCCGCAACACCCGGTAGAACCGCAGCCGCAGGATCAGCAGCCGCATCATCCGGTTGCGCCGCAGCCGCCGGAGCAGCAGCCGCATTATCAGCAGACGcctca                                                                                                                        >  6:295990/1‑136 (MQ=18)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACCACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCACCGCTTCCACCACAGCCGCAGTATCAGCAGCCGCAAAATCACCCGCAGccaca                                                                                                                        >  4:455613/1‑140 (MQ=18)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGACCCGCAGCCGCAGTATCAGCAGCCGCAATAGCAACAGCCGcaaca                                                                                                                        >  5:445872/1‑140 (MQ=255)
                     ccGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACAGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGcaaca                                                                                                                        <  8:713122/140‑1 (MQ=255)
                       gCAACACCCGGTAGCACCGCAGCCGCAATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGAATCAGCAGCCGCAACAACCGAGGAGACCACAGCCGCAGGAGCAGCAGCAGCAAAagccgcagccgcctcagc                                                                                                                      >  2:87941/1‑134 (MQ=9)
                       gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCTCAGCCGCAGGATCAGCAGCGGCAAGATCCGCAGCCGccacagc                                                                                                                      >  2:455190/1‑138 (MQ=14)
                       gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCCGTATCAGCAGCAGCAACATCCGGTTGCACCACAGCCGCAGGATCAGCAGCCGCAAGAGCACCAGCCGCcacaac                                                                                                                      >  2:460383/1‑140 (MQ=18)
                       gCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGcaacagc                                                                                                                      >  2:15007/1‑138 (MQ=255)
                              ccGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATACGCAGCCGCAAAAGCCGCAGCCGCCACAACCGGATGc                                                                                                               >  6:144495/1‑140 (MQ=18)
                               cGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCa                                                                                                              >  1:339850/1‑140 (MQ=255)
                                 gTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTCCACCGCAGCCGCAGTATCCGCAGCCGCAAAATCAGGAGCCGCCACAACCggagccacc                                                                                                            >  2:144512/1‑133 (MQ=18)
                                    ccACCGCCGCAGAAATATATTCCGCAGCAACAACCGCTATCACCGCAGACGCAGTCTCAGCAGCCTCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCTATATCAACAGCCGCAACAACCGGTTGCACCgca                                                                                                         <  2:91882/139‑1 (MQ=21)
                                     cACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCAGCAGCAGCATAATCAGCAGCCGCAACAGCCGGAAGCCCCgcag                                                                                                        >  4:47806/1‑140 (MQ=18)
                                      cccGCAGCAGCAAAATCACCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCgcagc                                                                                                       <  2:780496/139‑1 (MQ=255)
                                          cagcagcaATATCAGCAGCCGCAACACCCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCAGGTTGGCCCTCAGCCGCAGTATCAGCAGCCTCAATATCAGCCGCCGCAACAACCGGATGAACCgccgcagcc                                                                                                   >  8:613630/1‑139 (MQ=21)
                                          cagcagcaATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAACAGCCGCAACAACCGGTTGCACCgcagcagca                                                                                                   >  6:108411/1‑140 (MQ=255)
                                                caATATCAGCAGCCGCAACAACCGCTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCGATATca                                                                                             <  7:56157/140‑1 (MQ=255)
                                                aaaTATCACCAGCAGGAACATCAGGAATCACCGCCGCCGCATTATCATCAGGCGCAAGAACCTGTTGCACCGCAGGCGCAGTATCTGCAGCCTCATTATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATca                                                                                             <  2:804093/139‑1 (MQ=21)
                                                  ttcgcagcagCCGGAACAACCGGAAGGACCGCAGCCGCAGGCTCAGCAGCCGCAACATCCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagc                                                                                           <  8:554980/136‑1 (MQ=21)
                                                  atatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACCACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCATCAGCAGCAACAACCGGTTGCACCGCAGCAGCAAAATcagc                                                                                           >  5:613187/1‑140 (MQ=21)
                                                  atatCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATcagc                                                                                           >  1:170458/1‑140 (MQ=255)
                                                     tCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAAAATCAACAGCCGCAACAACCGGTTGCACCGCAGCCGCAATATCagcagc                                                                                        >  5:575577/1‑140 (MQ=255)
                                                       agcagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGca                                                                                                                           >  7:95776/1‑103 (MQ=255)
                                                         cagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGTATCCGCAGCAGCATAATCAGCAGCCGCCACAACCGGATGGCCAGCAGCTGCAAGATcagccgaccca                                                                                    >  7:158152/1‑133 (MQ=17)
                                                         cagcCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCa                                                                                                                                          <  8:627448/86‑1 (MQ=255)
                                                          agcCGCAACAACCGGTAGCACCGCATCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaa                                                                                   <  5:840671/140‑1 (MQ=255)
                                                          agcCGCAACAACCGGTAGCACCGCAGCCGCAGAATCAGCAGCCGCAACAGCCGGTTGCACCGCAGCCGCAGAATCAGCAGCAGCAAAATCAGCAGCCGCCACATCCGGATGCACCGCAGCAGCAAAATCAGCCGCCccaa                                                                                   >  1:290968/1‑140 (MQ=18)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  5:224260/140‑1 (MQ=255)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  2:339850/140‑1 (MQ=255)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  6:573186/140‑1 (MQ=255)
                                                            ccGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGGAGCACCACAGCCGCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCacaaca                                                                                 <  7:703920/140‑1 (MQ=255)
                                                             cGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAg                                                                                >  2:666265/1‑140 (MQ=255)
                                                                       ggTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCATCAGCCGCAACAACCGGTTGAACCGCAGCTGCATAATCAGCAGACACAACAGCCGGTTacac                                                                      >  1:644894/1‑140 (MQ=25)
                                                                       ggTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTacac                                                                      <  5:122692/140‑1 (MQ=255)
                                                                        gTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGATGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTAcaca                                                                     >  3:213414/1‑140 (MQ=255)
                                                                         tAGCACCGCATCCGAACTATCAGCAGCCGCATCAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcag                                                                    <  2:7018/140‑1 (MQ=255)
                                                                         aaGCACCGCCGCCGCAGTATGAGCAGCCTGAACTTCCCGTTGCACCGCAGCCGCCGCCTCAGCAGCCCCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACAcag                                                                    <  4:386902/139‑1 (MQ=25)
                                                                             aCCGCACCCGCAGTATCAGCAGCCGCAACCACCTGTTGCACCGCAGCCGCCGTCTAATCTTCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACagcagc                                                                <  6:918744/140‑1 (MQ=25)
                                                                              ccgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcc                                                               <  3:480323/140‑1 (MQ=255)
                                                                               cgcagccgcagTATCAGGAGCAGCACCAACCGGTTGAACCGCTGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGcct                                                              <  5:780650/140‑2 (MQ=37)
                                                                               cgcagccgcagTATCAGCAGCCGCAACCACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCCACAGCCGCCACACACGGTGTCCCCGCCGCCGCAATATCAGCGGCCACCACCGCCGGATAGCCCGCAGCCg                                                              >  7:609029/1‑140 (MQ=18)
                                                                               cgcagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGGATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                              <  1:13547/140‑1 (MQ=255)
                                                                                 cagccgcagTATCTGCTGCCTCAACTACCTGTTGCACCGCAGCAGAAGTCTCTTCAGCCGCATTATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                            <  1:601385/140‑1 (MQ=21)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGGACCGCAGCCGCAGGATCAGAAGCCGCATGATCACCATCCGCAACAACCGGTTGCACCGCAGCTGCAAGAGCAGCAGCACCAACAGCCGGGTACACATCAGCCGcc                                                            >  8:542588/1‑139 (MQ=25)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGATACACAgcagcagca                                                            >  4:267026/1‑140 (MQ=255)
                                                                                 cagccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                            >  7:307112/1‑140 (MQ=255)
                                                                                 cagacgaagTAGCAGCAGGCGCAACAACCTGTAGCACCGCAGCCGCAGTATCAGCAGCAGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCa                                                            <  1:253644/140‑1 (MQ=37)
                                                                                   gccgcagTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCCCCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAgg                                                          >  2:39280/1‑139 (MQ=255)
                                                                                              agcagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACAACCGGTGGCACCGCAGCTGCAATATCAGAAGCCGCCACAGCCGGATAAACAGAAGCAGCAGTATCAGCAGccc                                               >  4:260244/1‑139 (MQ=25)
                                                                                                cagcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCGCAACAGCCGGTTACGCAGCAGCAGCAGACTCAGCCGCCGCa                                             >  4:935863/1‑140 (MQ=25)
                                                                                                  gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAAc                                           >  2:838104/1‑140 (MQ=255)
                                                                                                  gcCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATAGCAACAGCCGCAACAGCCGGTTGCACCGCAGCAGCATAATCAGCAGCAACAACAGCCCGTTACACAGCAGCCGCAGTATCCGCAGCCGCaaa                                           >  7:289228/1‑139 (MQ=38)
                                                                                                    cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAGCAGCCGCTAAATCCGGATGCACCGCAGCAGCAATATCAGCAGACACCACAGCCGGGTACACAGCAGCCGCAGCAGCAGCAGCCGCAACAg                                         >  4:655104/1‑140 (MQ=25)
                                                                                                    cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTAGCAGCAGCAGCATCAg                                         >  5:376568/1‑140 (MQ=255)
                                                                                                    cGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGACGGTTACCCAGCAGCAGCAGTATCAGCCGCCGCAACAg                                         >  2:321173/1‑140 (MQ=255)
                                                                                                     gCAACTACCGCATGCACCGCAGCCGAAGTATCAGAAGCCGCAATATCAACACCCTCAACACACGTTTGCAACGCAGCAGCAATATCAGCAGCCACACCAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGc                                        <  8:168472/140‑1 (MQ=21)
                                                                                                      catcaacCGGACGCACCGCAGCCGCCGCATCTGCAGCCGCAATATCAACATCAGCAACACCCTGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGcc                                       <  6:62697/137‑1 (MQ=18)
                                                                                                         caacCGGTTGCACAGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGt                                    <  7:46763/140‑1 (MQ=255)
                                                                                                              ggTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCCACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACACCAGCCGGTTACACAGCAGCCGCAGTATCCGCAGCCGCAACCGCCGGTTGGAc                               >  1:650261/1‑140 (MQ=25)
                                                                                                                 tGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGAAGCCACAACAGCCGGTTACACAGCAGCCGCAGAATCAGCAGCCGCAACAGCCGGATGTACcac                            >  8:721572/1‑140 (MQ=255)
                                                                                                                 tGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGAACCGCAGCAGCAATATCAGCAGCCACAACAGCAGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACcac                            >  5:814458/1‑140 (MQ=255)
                                                                                                                     ccgcagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                        <  4:808950/140‑1 (MQ=255)
                                                                                                                     ccgcagccgaagTCTCAGCAGCAGCAATATCATCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGcc                        <  5:158059/140‑1 (MQ=255)
                                                                                                                       gcagccgcagTATCAGCTGGAGCAATATCACCAGCCTAAACTCCCGGTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCCGCAGCCGCAACAGCCGGTTGTACCACAGCCGc                      <  8:339836/140‑1 (MQ=21)
                                                                                                                       gcagccgcagTATCAGCAGCCGCAATATCACCAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGc                      <  8:339838/140‑1 (MQ=255)
                                                                                                                        cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     >  3:106780/1‑140 (MQ=255)
                                                                                                                        cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATCTCAGCAGCCACAACAGCCGGTTACTCAGCAGCCGCAGTATCAGAAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     >  4:217281/1‑140 (MQ=255)
                                                                                                                        cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCCGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGcc                     >  2:377595/1‑139 (MQ=255)
                                                                                                                        cagccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTAAACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     >  8:718212/1‑140 (MQ=255)
                                                                                                                        cagccgcagTATCAGCAGCAGCAATATCACCAGCCGCAACAACCGCTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCa                     <  7:216470/140‑1 (MQ=18)
                                                                                                                          ggctaattataAGCAGCCTCATTATCATCAGCCGCAACAACCGGTTTCACCGCTGCAGCAATATCAGCAGCCACAACATCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   <  3:543431/133‑1 (MQ=18)
                                                                                                                          gccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   <  8:505491/140‑1 (MQ=255)
                                                                                                                          gcagcagAATCAGCAGCAGCATGTTCATCAGCCGCACCATCAGCTTGCACCGCAGCAGCAATATCAGCAGCCTCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAgg                   <  6:169308/137‑1 (MQ=17)
                                                                                                                           ccgcagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCg                                                              <  4:111118/96‑1 (MQ=255)
                                                                                                                              cagTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACCGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAc               >  6:502742/1‑140 (MQ=255)
                                                                                                                                gTATCAGCAGCCGCAATATCAACACCCGCAACACACGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATAccc             <  4:826089/140‑1 (MQ=255)
                                                                                                                                    catgaggcGCAATTTCAACAGCAGCATCATCCGCTTGCACCGCAGCAGCAATATCAGCAGCCCCAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCt         <  3:848971/133‑1 (MQ=21)
                                                                                                                                     agcagcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCtt        >  1:481368/1‑140 (MQ=255)
                                                                                                                                         gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    <  2:468636/140‑1 (MQ=255)
                                                                                                                                         gcCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    >  1:468636/1‑140 (MQ=255)
                                                                                                                                         gcCGAAATATCAACAGCCGCAACAAACGGTTGCACAGCAGCAGCAATATCAGCAGCCACAACAGCAGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATc    <  3:724606/140‑1 (MQ=255)
                                                                                                                                           cGCATTATCAACAGCCGAATCATCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCATTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  <  1:559929/140‑1 (MQ=25)
                                                                                                                                           cGCAATATCAACAGCCGCAACACCCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCg  >  7:588409/1‑140 (MQ=255)
                                                                                                                                           cGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCCGCAGCCGCAACAGCCGGTGGTACCACAGCCGCAGGATACCCTGCTTCCTCCg  >  8:806082/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATTGTTGAACCTGTACAGCAGCCGCAACAACCGGTAGCACCGCAGCAGCAATATCAGCAGCCGCAACAACCGGTAGCACCGCAGCCGCAGTATCAGCAGCCGCAACAACCGGTTGCACCGCAGCCGCAGTATCAGCAGCCGCAATATCAACAGCCGCAACAACCGGTTGCACCGCAGCAGCAATATCAGCAGCCACAACAGCCGGTTACACAGCAGCCGCAGTATCAGCAGCCGCAACAGCCGGTTGTACCACAGCCGCAGGATACCCTGCTTCATCCG  >  NZ_CP022686/4440185‑4440463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A