Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F2 I0 R1 122 5985.5 7364544 98.3% 7239346 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.96.0% 754.3 / 21.9 173intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base . (84/82);  total (88/85)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 178 total aligned reads displayed.

ACGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCT  >  NZ_CP022686/4870773‑4871043
                                                                                                                                           |                                                                                                                                   
  gTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcca                                                                                                                                   >  3:113153/1‑139 (MQ=255)
   tCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAGccaa                                                                                                                                  >  1:406427/1‑138 (MQ=255)
    cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  4:96587/1‑140 (MQ=255)
    cTTGCACATCATTGATCAATATTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGATCAACGACACGGCGAACACTGACTGCCGATGAATCCATTTTGCTTCAGGCAAGGGAAAAGAAAATTAGCCTaa                                                                                                                                 >  4:254636/1‑140 (MQ=255)
      tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  6:394346/1‑138 (MQ=255)
      tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 <  6:103421/138‑1 (MQ=255)
      tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTAAACGACACGGCGACCACTGACTGCCGATCAATCCATTGTGCATCAGGCAAGGGAAAATAAAATTAGCCaaa                                                                                                                                 >  5:63756/1‑138 (MQ=255)
       gCACATCATTGATAAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGTCATGGATGATGTTCAACGACACGGCGACCACTGCCTGACGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                             >  3:234663/1‑140 (MQ=255)
             catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttta                                                                                                                       >  1:410014/1‑139 (MQ=255)
             catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  7:845205/1‑140 (MQ=255)
             catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  8:426953/1‑140 (MQ=255)
             catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  2:438088/1‑140 (MQ=255)
              atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                        <  5:739461/138‑1 (MQ=255)
              atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                      <  7:256958/140‑1 (MQ=255)
              atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                      <  2:831433/140‑1 (MQ=255)
               ttGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGAGGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCAGTGTGAATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  5:20058/1‑138 (MQ=255)
                  aTCTATTTTTTCAATATTTAGCTGCTGTAATTAGGGATGGGCATGGTTGTTGTTCTACGACACGGCGACGACTGACTGCTGATGAATCCATTGTGCATAAGGCAAGTGTAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                  <  4:290268/140‑1 (MQ=255)
                  aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                  >  7:438279/1‑140 (MQ=255)
                  aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                  >  8:855140/1‑140 (MQ=255)
                    cAATTTTTTCATTATTTTGCTCCATCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCTACCACTGACTGCCGATGAATCAATTGTGCATCAGCCAAGGGAAAAGAAAATTAG‑CCAAATTATGTTTCATAGt                                                                                                                  >  6:826707/1‑138 (MQ=255)
                    cAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCGTAGt                                                                                                                  <  5:61305/138‑1 (MQ=255)
                    aaaTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGa                                                                                                                <  3:578829/139‑1 (MQ=255)
                       ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                             >  3:453752/1‑140 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                             <  6:618472/138‑1 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  2:454267/140‑1 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  2:729698/140‑1 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCAGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                             >  6:668505/1‑138 (MQ=255)
                             cATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCTATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGc                                                                                                       <  3:784174/140‑1 (MQ=255)
                              attattTTGCTCAAGCAATTACGGTAGTGCATGGATGATGTTCAACTACACGTCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCt                                                                                                      <  1:550857/140‑1 (MQ=255)
                                   tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                 <  2:330471/140‑1 (MQ=255)
                                     tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                               <  7:637732/140‑1 (MQ=255)
                                     tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                 <  6:437769/138‑1 (MQ=255)
                                           aGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                         <  3:362440/140‑1 (MQ=255)
                                            gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCAGCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                        >  7:148784/1‑140 (MQ=255)
                                            gCAATTACGGTAGGGCATGGAGGATGTTCAACGACACGGCGACCACTGACTGCAGATGAATCCATTGTGCATCAGCCAAGGGAAAAGAAAATTAG‑CAAAATGACGTTTCATAGTGAAACATATGCGTTAATGAATGTTcc                                                                                        >  3:557443/1‑140 (MQ=255)
                                              aaTTAAGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGATTTAATGAATGTTcc                                                                                        >  5:455569/1‑138 (MQ=255)
                                                  aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                  <  4:923141/140‑1 (MQ=255)
                                                      tAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAc                                                                              >  1:947516/1‑140 (MQ=255)
                                                        gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGATACATATGCTTTAATGAATGTTCCAGATTGaacc                                                                              >  6:320622/1‑136 (MQ=255)
                                                        gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAc                                                                              >  5:595469/1‑138 (MQ=255)
                                                         ggCATGGATGATGTTCAACGACACTGCGACCACTGACTGCCGATGAATCCATTGTGCATCATGCAAGGGAAAAGAAAATTAG‑CCAAATAAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACttt                                                                           >  1:686725/1‑140 (MQ=255)
                                                          gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtt                                                                            >  6:178458/1‑138 (MQ=255)
                                                             tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGTATCCATTGTGCATCAGGCAAGGGAAAAGAAAAATAG‑CCAAATTAGGTTTCATAGTGAATCATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  1:674450/140‑1 (MQ=255)
                                                             tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  4:825989/140‑1 (MQ=255)
                                                              ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                      >  1:372981/1‑140 (MQ=255)
                                                              ggATGATGTTCAACGACACGGCGACCACTGACTGCCAATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                      >  8:120605/1‑140 (MQ=255)
                                                              ggATGATGTTCAACGACACGGCGACCACTGACTGCAGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATATTGAAACATATGCTTTAATGAAAGTTCCATAATGAAAATTTTACgt                                                                      >  8:246368/1‑140 (MQ=255)
                                                              ggATGAAGTACAACGACACCGCGACCACAGACGGCCGATGAATCCATTGTGCCTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGGTTCATAGTAAAACATATGCTTTAAGGAATGATCCAGATTGAAACGTTTACgg                                                                      >  4:504837/1‑139 (MQ=255)
                                                                atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTATGTTTCATAGTGAAACATATGATTTAATGAATGTTACATATTGAAACTTTTACgt                                                                      >  6:896231/1‑138 (MQ=255)
                                                                  gatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAt                                                                  <  4:637861/140‑1 (MQ=255)
                                                                    tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAt                                                                  <  5:893663/138‑1 (MQ=255)
                                                                    tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  8:587602/1‑140 (MQ=255)
                                                                    tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  1:310993/1‑140 (MQ=255)
                                                                    tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CAAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  1:717916/1‑140 (MQ=255)
                                                                     gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                               >  4:674942/1‑140 (MQ=255)
                                                                      ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGCAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGAATTa                                                                >  6:810529/1‑138 (MQ=255)
                                                                      ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                              >  2:275490/1‑140 (MQ=255)
                                                                      ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAATAGGTTACATAGTGAAACATATTCTTTAATGAATGTTCCATATTGAAACTTATACGTGTATTa                                                                >  5:92712/1‑138 (MQ=255)
                                                                         aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                           >  4:872186/1‑140 (MQ=255)
                                                                         aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                           >  4:787322/1‑140 (MQ=255)
                                                                                     aCCACTGACTGCCGATGAATCCATTGTGCATCAGTCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                               <  1:242449/140‑1 (MQ=255)
                                                                                         ctgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATTAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCgg                                           <  1:605091/140‑1 (MQ=255)
                                                                                            actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAac                                        <  2:146132/140‑1 (MQ=255)
                                                                                             ctgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                         >  5:694537/1‑138 (MQ=255)
                                                                                                ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                    >  8:341441/1‑140 (MQ=255)
                                                                                                ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                    >  1:261217/1‑140 (MQ=255)
                                                                                                ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGAGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTTATACTTAAAATTGCGAGCCGGAACACCt                                    >  3:73765/1‑140 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATCTTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  8:543789/140‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                     <  6:209563/138‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                     <  6:529042/138‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                     <  6:595469/138‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                     <  5:189199/138‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  8:752420/140‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   >  1:910190/1‑140 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  7:494842/140‑1 (MQ=255)
                                                                                                   aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATAAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATAGCGAGCCGGAACACCtttt                                 <  7:355469/140‑1 (MQ=255)
                                                                                                          cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                <  3:937143/102‑1 (MQ=255)
                                                                                                            ttGTGCATCAGGCAAGGGAAAAGAAAATTA‑TCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAgg                        <  8:295872/140‑1 (MQ=255)
                                                                                                            ttGTGCATCAGGAAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                          <  6:300915/138‑1 (MQ=255)
                                                                                                              gtgCATCAGGAAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                      <  2:68167/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGTGAAAATAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  7:572364/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAATAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  2:130667/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTCG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                      <  6:906978/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGACGGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  8:705773/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                      <  5:865692/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                      <  5:269569/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  4:567273/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  1:82952/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTGCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                      <  6:177896/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTGCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  4:558759/140‑1 (MQ=255)
                                                                                                                    cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                >  3:24491/1‑140 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                >  5:170619/1‑138 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              >  1:465543/1‑140 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              <  2:195833/140‑1 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              >  8:227129/1‑140 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              >  2:54246/1‑140 (MQ=255)
                                                                                                                       gCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACCCCTTTTGTCATAAGGGATGCGGGata             >  7:118326/1‑140 (MQ=255)
                                                                                                                         aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGaga             >  6:240307/1‑136 (MQ=255)
                                                                                                                            ggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGt        >  7:289441/1‑140 (MQ=255)
                                                                                                                                 agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc   >  3:349541/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                   
ACGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCT  >  NZ_CP022686/4870773‑4871043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A