Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F2 I0 R1 117 5184.3 6857682 98.3% 6741101 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.92.7% 617.0 / 43.9 151intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (10/0);  new base . (75/65);  total (85/66)
Fisher's exact test for biased strand distribution p-value = 5.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 151 total aligned reads displayed.

CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTT  >  NZ_CP022686/4870777‑4871046
                                                                                                                                       |                                                                                                                                      
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  5:700643/1‑140 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTAGATCCAGCAATTACGGTAGGGCATGGATCATGTTCAACGACACGGCGACCACTGCCTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  3:782031/1‑140 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  8:336127/1‑138 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  7:799366/1‑138 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                 <  4:397821/140‑1 (MQ=255)
   gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                                >  3:427941/1‑140 (MQ=255)
    cacaTCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGTGCATGGATGATGTTCAACGACACGGCGACCACTGTCTGCCGATGAATCCATTGTGCAACAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                 <  2:73000/138‑1 (MQ=255)
     acaTCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAgg                                                                                                                              >  3:610454/1‑140 (MQ=255)
     acaTCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATAAG‑CCAAATTa                                                                                                                                >  8:170150/1‑138 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttta                                                                                                                          >  3:584107/1‑139 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttta                                                                                                                          >  3:528648/1‑139 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  5:637456/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  6:351554/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGAGGATGTTCAACGACAAGGCGACAACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  6:333013/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCAAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGATAAGAAAATTAG‑CAAAATTAGTTTTc                                                                                                                          >  5:224248/1‑140 (MQ=255)
          atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                           <  7:804140/138‑1 (MQ=255)
           ttGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  2:774612/1‑138 (MQ=255)
           ttGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCAGTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGtata                                                                                                                          >  8:288919/1‑135 (MQ=255)
            tGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                       >  6:791303/1‑140 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCTTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                       <  1:645026/138‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                       <  1:259841/138‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     <  5:238744/140‑1 (MQ=255)
                cAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCTGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     >  7:742994/1‑138 (MQ=255)
                cAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     >  8:244713/1‑138 (MQ=255)
                cAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCAGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     >  1:469300/1‑138 (MQ=255)
                cAATTTTTTCATTATATTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTTCTGATGAATCCATTTTGCATCAGTCAAGTGAAAAGAAAATTAG‑CCAAATAAGGATTCATAGt                                                                                                                     >  7:742992/1‑138 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                >  6:203226/1‑140 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCAGGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGCCTCCAGAGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CGAAATAAGGATTCATAGTGCAAc                                                                                                                >  6:413646/1‑140 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGAGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                <  1:411258/138‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                  <  6:422792/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                  <  6:637456/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                    <  8:526738/138‑1 (MQ=255)
                                      cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGtt                                                                                             <  3:26664/140‑1 (MQ=255)
                                       aGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGt                                                                                              <  2:12208/138‑1 (MQ=255)
                                        gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                           >  3:657891/1‑140 (MQ=255)
                                              aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                     <  6:747970/140‑1 (MQ=255)
                                               cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTTACTGCAGATGAATCAATTGTGCATCAGTCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTACATAtt                                                                                      >  8:754931/1‑138 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTGTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAc                                                                                 >  1:211138/1‑138 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAc                                                                                 >  2:567305/1‑138 (MQ=255)
                                                     ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                              >  5:835511/1‑140 (MQ=255)
                                                      gCATGGATGATGTTCACCGACACGGCGCCCACGGACTGCCGATGAATCCATAGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGTTTTCATAGAGAAACATATGCTTTAAAGAATGTTCCATATAGAAAAtt                                                                               >  2:412209/1‑138 (MQ=255)
                                                       cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                           >  3:719816/1‑93 (MQ=255)
                                                       cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                           <  4:719816/93‑1 (MQ=255)
                                                       cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                              <  7:469427/138‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            <  2:24693/138‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  5:351554/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAAATATGCTTTAATGAATGTTCCAGATTGAAACTGTTACg                                                                          >  4:522140/1‑140 (MQ=255)
                                                         tGGATGATGGTCACCGCCACGGCGACCACTTACTGCCGATGAATCCATTGTGCCTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAATCATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            <  8:540512/138‑1 (MQ=255)
                                                           gatgatGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          >  2:700955/1‑138 (MQ=255)
                                                            atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                       >  2:396913/1‑92 (MQ=255)
                                                            atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCGATATTGAAACTTTTACgt                                                                         >  2:867282/1‑138 (MQ=255)
                                                               atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATAAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGCTTTCAAAGTGAAACATATGCTTTAATTAATGATCCATATTGAAACTTATAAGTGTAtt                                                                    >  4:493208/1‑140 (MQ=255)
                                                                 gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                    >  1:321732/1‑138 (MQ=255)
                                                                 gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTACATATTGAAACTTTTACGTGTAtt                                                                    >  7:368573/1‑138 (MQ=255)
                                                                  ttCATCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCTTATTGAAACTTTTACGTGTAttt                                                                   >  1:701795/1‑137 (MQ=255)
                                                                  ttCAACGACACGGCGACCACTGACTGCCGAAGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                   >  7:253081/1‑138 (MQ=255)
                                                                     aaCGCCACGGCGCCCACTCAATGCCGATGAATCCATTGTGCATAAGGCAAGGTAAAAG‑AAAGTAACCCAAGTTAGGTTTCTTAGTGAAACATATGCTTTAATCAATGTACAAAATTGAAACTTTTAGGTttattattagt                                                              >  6:779230/1‑138 (MQ=255)
                                                                            cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaca                                                         >  8:414384/1‑136 (MQ=255)
                                                                              gCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAtt                                                       >  1:388232/1‑138 (MQ=255)
                                                                               cgcccACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTg                                                      >  8:261705/4‑138 (MQ=255)
                                                                                gACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCgc                                                   >  5:680491/1‑139 (MQ=255)
                                                                                   cACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                  >  7:265383/1‑138 (MQ=255)
                                                                                     ctgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCgg                                              >  4:220650/1‑140 (MQ=255)
                                                                                      tgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCg                                               >  1:74929/1‑138 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                            <  6:799065/140‑1 (MQ=255)
                                                                                        acttCCGATGAATCCATTGTGCATCAGGCAACGGAAAAGAAAATTA‑CCCAAAATAGGTAACATAGTGAAACATATGCTTAAATGAATGATCCATATTGAAACTTTTACGTGAATTAATAATTAAAAATGCCAGCCGCAac                                           >  4:834344/1‑140 (MQ=255)
                                                                                        actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                              <  6:505840/73‑1 (MQ=255)
                                                                                        actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGa                                             <  1:841298/138‑1 (MQ=255)
                                                                                             ctaTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGTTTTCTTAGTGAAACATATGCTTTCATGAATGTTCCATATTGAATCTGTTACGTGTATTTATACTTAAAATTGCGAGCCGGAACAcc                                        <  7:125845/136‑1 (MQ=255)
                                                                                             cGATGACTCCATTGTGCATCAGGCAAGGGAAAAGAACATTCG‑CCAAATTAGGTTTCATAGGGACACATATGCTTTAATGAATGTTCCATATTGAACCGTTTACGTGTATTACTACTTAAAATTGCGCGCCGGaccaccgg                                      >  6:814439/1‑138 (MQ=255)
                                                                                             cGATGAATCCCTATTGCATCAGGCAAGTGCAAAGAAAAATAG‑CCAACTTCGGTTTCATAGTGAAACATATGCTTTACTGACTGATCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                        <  8:615778/138‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAATGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATTAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                        <  7:113912/138‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAACCATATGCTTTACTGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                        <  1:189617/138‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATTTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                        <  7:662437/138‑1 (MQ=255)
                                                                                                 gAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGt                                  <  4:16992/140‑1 (MQ=255)
                                                                                                      cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                               >  8:3950/1‑138 (MQ=255)
                                                                                                        ttGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAgg                           <  4:491433/140‑1 (MQ=255)
                                                                                                          gtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                                 <  6:146785/100‑1 (MQ=255)
                                                                                                           tgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAggg                          >  1:798886/1‑138 (MQ=255)
                                                                                                           tgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATCTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCATTTGTCATAAggg                          >  8:124458/1‑138 (MQ=255)
                                                                                                            gcctgaGGCAAGGGAAAAGAAAATTAG‑CCTAATTATGTTTGATAGTGAAACATATGCTTTAATGATTTTTCCATATTGTAACTTTTACGTGTATTTATTCTTAAAATTGCGAGCCTGAACACCTTTTGTCATACGGGAtt                       <  3:257491/135‑2 (MQ=255)
                                                                                                            gCATCAGGCAAGTGAAAAGAAATTTAG‑CCAAATTAGGTTTAATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  1:260260/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGTTTTCATAGTGAAACATATGCTTTAATGATTGTTCCATATTGATACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  7:90690/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATTCTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  7:544834/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  7:543266/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  8:597475/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  5:835383/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  6:704910/140‑1 (MQ=255)
                                                                                                             cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGc                      >  5:79303/1‑140 (MQ=255)
                                                                                                                  ggaaaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTTTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                   <  7:94793/135‑1 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                 <  5:777645/140‑1 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTGTAATGAATGTTCCATATTGAACCTTTTAAGTCTATTAATACTTAAAATTGCGAGCCGGAACACGTTTTGTCAAAAGGGATGCGGGat                 >  4:219402/1‑140 (MQ=255)
                                                                                                                    cAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                 >  1:750464/1‑138 (MQ=255)
                                                                                                                     aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATg              >  3:600744/1‑140 (MQ=255)
                                                                                                                     aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATg              >  3:437670/1‑140 (MQ=255)
                                                                                                                       gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGACCACCTTTTGTCATAAGGGATGCGGGATATGAg            >  5:472489/1‑140 (MQ=255)
                                                                                                                       gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGatatt              >  7:708217/1‑137 (MQ=255)
                                                                                                                             agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc      >  4:522544/1‑140 (MQ=255)
                                                                                                                              taaaaTTAG‑CCAAATTATGTGTAATAGTGATACATATGCTTTAATGATTGTTCCTTATTGAAACTTTTACGTGTATTAATACTTAAAATTGTGAGCAGGAACAGCTTTTGTCATAAGGGTTGCGGGATATGAGTGgcg       <  2:518262/137‑1 (MQ=255)
                                                                                                                                 aaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtttt  >  4:319077/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTT  >  NZ_CP022686/4870777‑4871046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A