Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F13 I0 R1 230 5035.0 9125430 98.4% 8979423 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.92.4% 742.1 / 52.5 183intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (11/1);  new base . (99/70);  total (110/73)
Fisher's exact test for biased strand distribution p-value = 2.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 184 total aligned reads displayed.

CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCT  >  NZ_CP022686/4870777‑4871043
                                                                                                                                       |                                                                                                                                   
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  4:511196/1‑140 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGCTGAATCAATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcaaaa                                                                                                                                 >  5:254364/1‑136 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 <  5:716663/140‑1 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  2:398099/1‑140 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  5:254360/1‑140 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCCGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  8:720108/1‑138 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  7:1021288/1‑138 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                              <  5:124731/140‑1 (MQ=255)
   gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                             >  4:116559/1‑140 (MQ=255)
   gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTCCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                             >  1:282588/1‑140 (MQ=255)
       atcatTGATCAATTTTTTCATTATTTTCCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATCAATCCATTGTGAATCAGGCAAGGGAAAAGAAAATAAG‑CCAAATTAggat                                                                                                                         >  4:369317/1‑138 (MQ=255)
        tcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGTGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                        <  3:234327/140‑1 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  4:835009/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATAAGGTTTc                                                                                                                       >  4:858294/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CAAAATTAGGTTTc                                                                                                                       >  6:133364/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATCATGTTCAACGACACGGCGACCACTGACTGCCGATGAAACCATTGTGCATCAGGCAACGGAAAAGAAAATTAG‑CCAACTTAGGTTTc                                                                                                                       >  4:557207/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTACAGCAATTACGGTAGGGCATGGATTATGTTCAACGACAAGGCGACCACTGACTTCCGATGAATCAATTGTGAATCATCCAAGGGAAAAGAAAATTAG‑CAAAATTATGttag                                                                                                                       >  6:576334/1‑138 (MQ=255)
          atTGATGAATTTTTTAATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACTACACGGCGTCCTCTGACTGCCGATGACTCCATTGTGCATCAGGCTAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                      <  1:754247/140‑1 (MQ=255)
          atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                      <  2:80899/140‑1 (MQ=255)
           ttGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGAACACTGACTGCCGATGAATCCAATGTGCATCAGGCATGGGAAAAGAAAATTAG‑CCAAATAAGGttta                                                                                                                       >  7:843741/1‑137 (MQ=255)
            tGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                    <  6:733459/140‑1 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                             >  3:656988/1‑140 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGTCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATTAATCAATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                             >  7:269429/1‑138 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                             <  7:1065521/138‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  1:157016/140‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  4:710068/140‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCAGGGATGATGTTCAACGACACGGCGACACCTGACTGCCGATGAATCCATTGTTCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTTAAAc                                                                                                             >  8:278533/1‑138 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGAATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATTAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑ACAAATTAGGTTTCATAGTGAAAc                                                                                                             >  8:194885/1‑138 (MQ=255)
                     tttgCATTATTTTGCTCCACCAATTACGGTAGGGCATCGATGATGTTCAACTACACGGCGAACACTGACTGCCTATGAATCCATTGTGCATCAGGCAAGGGAAAAGAATATTCG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  1:322016/140‑1 (MQ=255)
                          attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATg                                                                                                        <  8:1021288/138‑1 (MQ=255)
                               tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                 <  1:426628/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                               <  6:847922/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                               >  1:86046/1‑140 (MQ=255)
                                      ccgCAATTACGGGAGGGCCTGGATGATCTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCCTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGtt                                                                                          <  2:113067/138‑1 (MQ=255)
                                      cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAACTAGGTTTCATAGGGAAACATATGCTTTAATGAATGtt                                                                                          <  4:942043/140‑1 (MQ=255)
                                      cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCAATGTGCATCATGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGGTTCATAGTGAAACATATGCTTTAATGAATGtt                                                                                          <  4:1088039/140‑1 (MQ=255)
                                       aGCAATTACGGTAGGTCATGGATGATGTTCAACGACACGGCGACCACTGCCTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                         <  2:612119/140‑1 (MQ=255)
                                       aGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGt                                                                                           <  7:929339/138‑1 (MQ=255)
                                          aaTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                        >  8:515045/1‑138 (MQ=255)
                                                 gTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGaaa                                                                               >  4:503749/1‑140 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                               <  6:221218/121‑1 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                 <  6:468853/119‑1 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                 >  5:468853/1‑119 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtt                                                                            >  5:567562/1‑140 (MQ=255)
                                                      gCATGGATGATGTTCAACGACACGGCGTCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtt                                                                            >  8:741910/1‑138 (MQ=255)
                                                      gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATTAATGTTCCATATTGAAACtt                                                                            >  8:1057032/1‑138 (MQ=255)
                                                         tGGATGATGTTCTACGACAAGGCGATCACTGACTTACGATGTATAGATTGTGCATCAGGCTAGGTTTATGTAAATTAG‑CCAAATTTGGTTTCATAGTGAATCATATGCTTTAATGAATGTGCCATATTGAAACTTTTACg                                                                       <  6:659907/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  5:85841/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTATACg                                                                       >  2:82831/1‑140 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                      >  3:805112/1‑140 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                      >  1:297028/1‑140 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                      <  1:1102596/140‑1 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgg                                                                      >  3:786248/1‑139 (MQ=255)
                                                           gatgatGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGATTGTTCCTTATTGAAACTTTTACg                                                                       >  8:822168/1‑138 (MQ=255)
                                                            atgatgTTCACCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTACTGAATGTTCCATATTGAAACTTTTACgt                                                                      >  7:665579/1‑138 (MQ=255)
                                                            atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                    >  1:854541/1‑140 (MQ=255)
                                                            atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                    >  3:45337/1‑140 (MQ=255)
                                                              gatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                    >  8:80087/1‑138 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  2:466942/1‑140 (MQ=255)
                                                                 gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                               >  1:799276/1‑140 (MQ=255)
                                                                  ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                              >  2:686377/1‑140 (MQ=255)
                                                                     aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                           >  6:561119/1‑140 (MQ=255)
                                                                         acacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTa                                                         >  7:145436/1‑138 (MQ=255)
                                                                               cGACCACTGACTGCCGATGAATCCATTGTGCATAAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGATACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                 >  2:256552/1‑140 (MQ=255)
                                                                               cGACCACTGACTGCCGATGAAGAGATAGTGCATCAGGCAAGGGAAAATAAAATAAG‑CCAAAATACGTGTCATACTGAAACATATGCTTTAATGAATGATCCATATTGAAACTTTTACGTGTATTAATACTTAAAAATGCg                                                 <  2:745601/140‑1 (MQ=255)
                                                                                  ccACTGACTGCAGATTAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTATATGAATGTTACATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGc                                              <  3:698592/140‑1 (MQ=255)
                                                                                        actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAac                                        >  6:7616/1‑140 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  2:420004/140‑1 (MQ=255)
                                                                                               aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAATGCGAGCCGGAACTCCtttt                                 >  1:782876/1‑140 (MQ=255)
                                                                                                  aaTCCATTGTGCATCAGGCAAGGGAAAAGAAAAATAG‑CCAAATTAGGTATCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGACGGAACACCTTTTGTc                              >  2:361456/1‑140 (MQ=255)
                                                                                                    tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                            >  3:963771/1‑140 (MQ=255)
                                                                                                      cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                          >  4:59257/1‑140 (MQ=255)
                                                                                                      cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                          >  1:1006256/1‑140 (MQ=255)
                                                                                                        ttGCGCCTCAGCCAAGGGCAAAGAAAATTAG‑CCAAAATAGGTTTAATAGTCAAACATATGCTTTAATGAATGTTCCATAATGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                          <  8:137747/138‑1 (MQ=255)
                                                                                                         tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  5:188668/92‑1 (MQ=255)
                                                                                                         tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       >  6:188668/1‑92 (MQ=255)
                                                                                                         tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAggg                       >  5:332666/1‑140 (MQ=255)
                                                                                                         tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAg                         >  7:844042/1‑138 (MQ=255)
                                                                                                         tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTAATAGTTAAACATATGATTTATTGATTGTTCCATATTGCAACGTGTACGTTTAATAAGACTTAATATTGCGAGAAGGAACACGTTTTTTCAGAAggg                       >  4:85661/1‑140 (MQ=255)
                                                                                                            gcagcaGGAAAGGGATAAGAAAATTAG‑CCAAATTATGTTTCATAGTGATACATATGCTTTAATGAATGTTACATATTGAAACTTTTAAGTGTATTAATACTTATAATTGCGAGCCGGAAAACCTTTTGTCATAAGGGATg                    <  6:394871/140‑1 (MQ=255)
                                                                                                            gCATGAGGCAAGGGAAAAGAAACTT‑CTCTAAATTAGGTTTCATAGTGATACATATGCTTTAATGAATGTTCCATTTTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACCCCTATTGTCATAAGGGATg                    <  5:845967/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGATAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  4:910115/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  1:594916/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  3:482652/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAATAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                      <  7:94485/138‑1 (MQ=255)
                                                                                                             cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGc                   >  3:142780/1‑140 (MQ=255)
                                                                                                              aTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACAAATTCATTAATGAATATTCCAAATTGAAAATTATAGCTGAATTAATACTTAAAATTGCGAGCCGGAACACATGTTGTCATAAGGCATGCg                  >  4:69147/1‑140 (MQ=255)
                                                                                                               tCAGGCTAGGGAAAAGAAAATTAG‑CCAAATTAGGATTCATAGAGAAACATATGCTTTAATGAATGTTACATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAGATTGTGTCATAATGGATGc                   >  8:329190/1‑138 (MQ=255)
                                                                                                                cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                >  2:26622/1‑140 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    >  2:855698/1‑134 (MQ=255)
                                                                                                                    cAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              >  7:204382/1‑138 (MQ=255)
                                                                                                                     aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATg           >  6:328333/1‑140 (MQ=255)
                                                                                                                       gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg         >  3:610220/1‑140 (MQ=255)
                                                                                                                       gggAAAAGAAAATTAG‑CCAAATTAGGTTTAATAGTGAAACATAAGCTTAAATGAAAGAACAATATAGAAAATTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAAAAGGCATGCGGGATATGAg         >  6:885615/1‑140 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg       <  3:1114116/140‑1 (MQ=255)
                                                                                                                          aaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGt        >  8:408287/1‑138 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg    >  1:968244/1‑140 (MQ=255)
                                                                                                                             agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc   >  4:400721/1‑140 (MQ=255)
                                                                                                                             agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc   <  3:255632/140‑1 (MQ=255)
                                                                                                                              gaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCt  <  5:312832/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCT  >  NZ_CP022686/4870777‑4871043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A