Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.93.7% 785.3 / 37.3 188intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (7/1);  new base . (96/80);  total (105/83)
Fisher's exact test for biased strand distribution p-value = 8.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 193 total aligned reads displayed.

ACGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870773‑4871047
                                                                                                                                           |                                                                                                                                       
aCGTCTTGCACATCATTTATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGTATGATGTTCAACGACACTTCGACCACTGACTGCCGATGAATCCCTTGTGCTTCAGGCAAGGGAAAAGAAAATTAGc                                                                                                                                         <  5:544405/140‑1 (MQ=255)
aCGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGc                                                                                                                                         <  7:321646/140‑1 (MQ=255)
    cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                     >  5:592953/1‑140 (MQ=255)
    cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                     >  7:795108/1‑140 (MQ=255)
    cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTAAGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                     >  7:681893/1‑140 (MQ=255)
      tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                     <  4:244958/138‑1 (MQ=255)
      tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  2:1084303/140‑1 (MQ=255)
      tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  7:588520/140‑1 (MQ=255)
       gCACATCATAGATCAATTTTTTCATTATTTTGCTCCAGCAATAACGTTAGGGAATGGATGATGTTCAACGACACGGCGACAACTGACTGCCGATTAATCCATTGTGCATCCGGCAAGGGAAAAGAAAATTAGccacatta                                                                                                                                  >  2:657312/1‑134 (MQ=255)
             catTGATCAATTTTTTCATTATTTTGCTCCAGCACTTACGGAAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCATGCAAGGGAAAAGAAAATTAG‑CCAAATAAGGttta                                                                                                                           >  7:1022288/1‑139 (MQ=255)
              atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGAGACGGCGACCACTGACTGCCGATGAATCCATTGTGGATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                            <  3:768801/138‑1 (MQ=255)
                  aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                        <  4:623717/138‑1 (MQ=255)
                  aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  6:186423/140‑1 (MQ=255)
                  aTCAATTATTTCATTATTTTTCTCCAGAAATTACGGTAGGGCATGGATGATGATCAACGACACGGCGACAACAGACTGCCGATGAATCCTTTGTGCATCACGCAAGGGAAAAGAAAATTA‑TCCAAATTAGGTATCATAGt                                                                                                                      <  7:445757/140‑1 (MQ=255)
                   taaaTTTTTTCATTATTATGCTCCAGCAATTACGGTAGGGCATGGATGATTTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCATGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAg                                                                                                                       <  3:240398/136‑1 (MQ=255)
                   tCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                     <  5:154914/140‑1 (MQ=255)
                       ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTTCATCAGGCAAGGGAAATGAAAATTAG‑CCAAATTAGTTTTCATAGTGAAAc                                                                                                                 >  2:472153/1‑140 (MQ=255)
                       ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaa                                                                                                                   >  4:42884/1‑138 (MQ=255)
                       ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  5:977756/1‑140 (MQ=255)
                       ttttttCAGTATTTTGCTCCAGCAATTACGGTAGTGCTAGGATCATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCCTCAGGCAACAGAAAAGAAAATTA‑TCCAAATTAGGTTTCATAgagcaaa                                                                                                                 >  7:841159/1‑134 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACTGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  2:1016952/140‑1 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  4:999174/1‑138 (MQ=255)
                         ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTCAAACat                                                                                                               <  2:221904/140‑1 (MQ=255)
                            tCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATg                                                                                                            <  2:492629/140‑1 (MQ=255)
                                     tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCTCTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  2:148073/140‑1 (MQ=255)
                                     tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                     <  3:220951/138‑1 (MQ=255)
                                           aGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                             <  6:571174/140‑1 (MQ=255)
                                            gCAATTTCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCATGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGtt                                                                                              <  4:730709/138‑1 (MQ=255)
                                            gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                            >  5:456584/1‑140 (MQ=255)
                                                  aCGGTCGGGCATGGCTGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                      <  2:277152/140‑1 (MQ=255)
                                                  aCGGTAGTGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                      <  6:240571/140‑1 (MQ=255)
                                                   cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGa                                                                                     <  1:149565/140‑1 (MQ=255)
                                                   cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGa                                                                                     <  6:127312/140‑1 (MQ=255)
                                                   cGGTAGGGCATGGATGATGTTCAACGACACGGAGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTATCATAGGGAAACATATGCTTTAATGAATGTTCCATATTGa                                                                                     <  8:315174/140‑1 (MQ=255)
                                                      tAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATAATGAAAc                                                                                  >  2:820690/1‑140 (MQ=255)
                                                             tGGATGATGTTCAACGACACTGCGACCACTGACAGCCGATGCAGCCATTGGGCATCAGGCAAGGGAAAAGAATATTAG‑CAAAATTAGGATTCATAGTGAAACATATGATTTAATGAATTTTCCAGATTGAAACTTTTACg                                                                           >  7:249241/1‑140 (MQ=255)
                                                             tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                             >  3:282148/1‑138 (MQ=255)
                                                             tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  8:681893/140‑1 (MQ=255)
                                                             tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           >  1:162316/1‑140 (MQ=255)
                                                              ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaa                                                                                                                   >  5:289186/1‑99 (MQ=255)
                                                              ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaa                                                                                                                   <  6:289186/99‑1 (MQ=255)
                                                                atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                        >  5:179898/1‑140 (MQ=255)
                                                                atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                          >  3:830776/1‑138 (MQ=255)
                                                                atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                          >  4:92471/1‑138 (MQ=255)
                                                                   atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTTAATCTTTTACGTGTAtt                                                                     >  1:369559/1‑140 (MQ=255)
                                                                   atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  7:302475/1‑140 (MQ=255)
                                                                   atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  2:381208/1‑140 (MQ=255)
                                                                   atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  2:94821/1‑140 (MQ=255)
                                                                    tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCAGAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  1:820752/1‑140 (MQ=255)
                                                                      ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCAGCAGGCAAGGGAAAAGAAAATTA‑TCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAATTTTACGTGTATTatt                                                                  >  5:633155/1‑138 (MQ=255)
                                                                         aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  1:461209/1‑140 (MQ=255)
                                                                         aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  1:949233/1‑140 (MQ=255)
                                                                           cGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGATACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTa                                                             >  2:351655/1‑140 (MQ=255)
                                                                           cGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  3:460327/1‑138 (MQ=255)
                                                                              cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGATTGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  8:281382/1‑140 (MQ=255)
                                                                                cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATAtt                                                                                       <  4:787459/109‑1 (MQ=255)
                                                                                cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGATACTTTTACGTGTATTAATTCTTaaaa                                                          >  3:271442/1‑138 (MQ=255)
                                                                                 ggCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTg                                                       <  6:8216/140‑1 (MQ=255)
                                                                                  gCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAttt                                                        >  4:494643/1‑138 (MQ=255)
                                                                                   cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                     <  8:415828/140‑1 (MQ=255)
                                                                                   cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                     <  2:1126597/140‑1 (MQ=255)
                                                                                           gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCgg                                               <  3:474576/138‑1 (MQ=255)
                                                                                           gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                             <  6:865908/140‑1 (MQ=255)
                                                                                            actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGa                                              <  4:1092116/138‑1 (MQ=255)
                                                                                            actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAac                                            >  8:121690/1‑140 (MQ=255)
                                                                                             ctgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAAATAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCAGATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGac                                             >  3:1072130/1‑137 (MQ=255)
                                                                                                ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                        >  1:1052483/1‑140 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                         <  4:591507/138‑1 (MQ=255)
                                                                                                 cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  6:209492/140‑1 (MQ=255)
                                                                                                   aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtttt                                     <  6:1040565/140‑1 (MQ=255)
                                                                                                     gAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtttt                                     >  3:721615/1‑138 (MQ=255)
                                                                                                     gAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGt                                   <  5:33129/140‑1 (MQ=255)
                                                                                                        tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                <  6:592953/140‑1 (MQ=255)
                                                                                                        tCCATTGTGCATCAGGCAAGGCAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTAACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                >  7:201455/1‑140 (MQ=255)
                                                                                                             tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAggg                           >  8:55048/1‑140 (MQ=255)
                                                                                                                tcATCAGGCAAGGGAAAAGAACATTAG‑CCAAATTAGGGTTCTTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGTATCACCTTTTGTCATAAGGGa                          <  4:470870/137‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTTAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                          <  3:630146/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CGAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  5:119089/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCTAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  6:45205/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                          <  3:450368/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                          <  3:196783/138‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  6:52294/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:126329/140‑1 (MQ=255)
                                                                                                                gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCAAAGTGAAACATATGCTTTTATGAATGTTCCATATTGAAACTTTTACGTGATTTAATACTTAAAATTGCGATCCGGAACACCTTTTGTCTTAAGGGATg                        <  1:749234/140‑1 (MQ=255)
                                                                                                                 cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGAt                         >  4:349895/1‑138 (MQ=255)
                                                                                                                   tCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACTTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTATGTCATAAGGGATGc                       >  3:977525/1‑138 (MQ=255)
                                                                                                                   tCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCgg                     <  1:241052/140‑1 (MQ=255)
                                                                                                                    cAGGCAAGGGAAAATAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCg                      >  4:56243/1‑138 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCGTATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       >  1:1141737/1‑119 (MQ=255)
                                                                                                                      ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                  >  2:593586/1‑140 (MQ=255)
                                                                                                                        cAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGCGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                  >  4:1078653/1‑138 (MQ=255)
                                                                                                                         aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGCACACCTTTTGTCATAAGGGATGCGGGATATg               >  2:264048/1‑140 (MQ=255)
                                                                                                                           gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg             >  7:755762/1‑140 (MQ=255)
                                                                                                                           gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTATTACGTGTATTAATACTTAAAATTGCTAGCCGGAACACCTTTTTTCATAAGGGATGCGGGATATg               >  3:892110/1‑138 (MQ=255)
                                                                                                                             gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTTCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  8:208590/140‑1 (MQ=255)
                                                                                                                             gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg             <  4:486706/138‑1 (MQ=255)
                                                                                                                                aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg        >  8:748946/1‑140 (MQ=255)
                                                                                                                                     aaTTAG‑CCAAATTAGGTTTCATAGTGAACCATATGCTTTAAGGAATGTTCCATATTGAAACTTTAACGTGTAATAATAATAAAAATTGCGAGCCGGACCACCAATTGTCATAAGGGATGCGGGATATGAGTGGCGCtatt   >  1:1003473/1‑140 (MQ=255)
                                                                                                                                     aaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGAGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTAATAAGGGATGCGGGATATGAGTGGCGCtttt   >  5:560446/1‑140 (MQ=255)
                                                                                                                                      aTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGATTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTGTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTa  >  7:889665/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
ACGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870773‑4871047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A