Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.95.6% 670.6 / 14.9 156intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base . (76/73);  total (80/76)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 160 total aligned reads displayed.

CGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTT  >  NZ_CP022686/4870774‑4871046
                                                                                                                                          |                                                                                                                                      
cGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcc                                                                                                                                       <  5:255095/140‑1 (MQ=255)
   cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTTGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  5:966088/1‑140 (MQ=255)
   cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  7:963301/1‑140 (MQ=255)
   cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  1:781339/1‑140 (MQ=255)
   cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGGTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcca                                                                                                                                      <  3:426555/138‑2 (MQ=255)
     tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGAAGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  4:1009588/1‑138 (MQ=255)
      gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGCCACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcaaaatta                                                                                                                                 >  1:372468/1‑133 (MQ=255)
            catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  6:1118950/1‑140 (MQ=255)
            catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  2:840829/1‑140 (MQ=255)
            catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  8:108894/1‑140 (MQ=255)
               tGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                         >  4:440969/1‑138 (MQ=255)
                 aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                       <  4:571766/138‑1 (MQ=255)
                 aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     <  6:887188/140‑1 (MQ=255)
                  tCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATTATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                    <  6:750681/140‑1 (MQ=255)
                  tCAAGTTTTTCATTATTTTGCTCCATCAATTACGGGAGGGCATGGATGATGTTCAACGAAACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                    <  8:137312/140‑1 (MQ=255)
                      ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                >  6:607354/1‑140 (MQ=255)
                       tttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACa                                                                                                               >  2:1022271/1‑140 (MQ=255)
                        ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                <  4:227403/138‑1 (MQ=255)
                        ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                <  3:543694/138‑1 (MQ=255)
                             attattTTGCTCCTGCAATTACGGTAGGGCATGGATGATGTTCATCGACTCGGCGACCACTGACTGCCGATGAATCAATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATg                                                                                                           <  3:399729/138‑1 (MQ=255)
                             attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCt                                                                                                         <  2:960306/140‑1 (MQ=255)
                             attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCt                                                                                                         <  2:323977/140‑1 (MQ=255)
                                  tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                    <  5:936944/140‑1 (MQ=255)
                                    tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                    <  4:828443/138‑1 (MQ=255)
                                    tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                    <  4:921278/138‑1 (MQ=255)
                                    tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGAAACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                    <  3:708766/138‑1 (MQ=255)
                                          aGCAATTACTGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                            <  8:494871/140‑1 (MQ=255)
                                           gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAATAAATTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                           >  6:821935/1‑140 (MQ=255)
                                           gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                           >  1:676216/1‑140 (MQ=255)
                                                  cGGTAGGGCATGGATTATGATCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGa                                                                                    <  7:1046331/140‑1 (MQ=255)
                                                        ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                              >  1:576501/1‑140 (MQ=255)
                                                         gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATACATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑ACAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGataatt                                                                               >  3:305254/1‑133 (MQ=255)
                                                          cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                              >  3:765313/1‑138 (MQ=255)
                                                          cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            >  2:191604/1‑140 (MQ=255)
                                                          cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            >  6:546809/1‑140 (MQ=255)
                                                            tGGTTGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCGATTGTGCATCAGGCAAGGGATAAGAAAATTCG‑GCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  1:32610/140‑1 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  2:880600/140‑1 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  5:371704/140‑1 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  1:92186/140‑1 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTGTAATGAATGTTCCATATTGAAACTTTTa                                                                            >  4:1045200/1‑138 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGGTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  8:799463/140‑1 (MQ=255)
                                                            gggATGATGTTCAACGACACGGCGACCACTGACTGCCGCTGAATGCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  6:349268/139‑1 (MQ=255)
                                                               atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                       >  6:955265/1‑140 (MQ=255)
                                                                  atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                    >  2:892392/1‑140 (MQ=255)
                                                                   tgTTCAACTACCCGGCTACCACTGACTGCAGAAGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTA‑TGCAAATTCGGTATCATAGTGAAACTTATGCTTTTATGATTGTTCCATATTTAAACTTTTACGTGTATTa                                                                   <  2:734282/140‑1 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCTTATAGAAACTTTTACGTGTATTa                                                                   >  7:410223/1‑140 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGACGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATAAGCTTTAATGAATGTTCCATATTGATACTTTTACGTGTATTa                                                                   >  1:311017/1‑140 (MQ=255)
                                                                     ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTttatta                                                                   >  4:120688/1‑138 (MQ=255)
                                                                      tCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                                  >  4:939047/1‑138 (MQ=255)
                                                                      tCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                                  >  3:1147085/1‑138 (MQ=255)
                                                                       cAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                              <  2:140344/93‑1 (MQ=255)
                                                                       cAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                              >  1:140344/1‑93 (MQ=255)
                                                                        aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                              >  6:584888/1‑140 (MQ=255)
                                                                        aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                              >  1:215221/1‑140 (MQ=255)
                                                                         aCGACACGGCGACCACTGACTGCCGATGAATCCATTTTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCGTGAATGAATGGTCCATATTGAAACTTTTAAGTGAATTAATACtt                                                             >  8:544143/1‑140 (MQ=255)
                                                                             cacGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAATTTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                         >  1:1073633/1‑140 (MQ=255)
                                                                             cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTATACTTGTATTAATACTTaaaa                                                         >  7:616381/1‑140 (MQ=255)
                                                                             cacGGCGACCACTGACTGCCGATGAATCCATTGTGCAGCAGGCAAGGGTAAAGAAAATTAG‑CCAAATTAGGTTCCATAGTGAAACATATGATTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                         >  5:996914/1‑140 (MQ=255)
                                                                               cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                         >  4:211346/1‑138 (MQ=255)
                                                                               cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAtt                                                       >  8:604573/1‑140 (MQ=255)
                                                                                  cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGGAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTATTGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTATAATTg                                                      <  3:176775/138‑1 (MQ=255)
                                                                                  cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                    >  5:684387/1‑140 (MQ=255)
                                                                                    ccccctgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAACAAACTTA‑TCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                  <  5:633546/136‑1 (MQ=255)
                                                                                    aCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                  >  6:34087/1‑140 (MQ=255)
                                                                                     ccACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGa                                                   <  4:1128051/138‑1 (MQ=255)
                                                                                         tgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTATAATTGCGAGCCGGa                                             >  8:109011/1‑140 (MQ=255)
                                                                                          gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                            <  5:183143/140‑1 (MQ=255)
                                                                                          gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                            <  2:1126823/140‑1 (MQ=255)
                                                                                               ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAacac                                         <  4:860734/138‑1 (MQ=255)
                                                                                               ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGACCACCt                                       >  2:515174/1‑140 (MQ=255)
                                                                                                cGATGAATCCTTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                        <  3:830755/138‑1 (MQ=255)
                                                                                                cGATGAATCCATTGTGCATCAGGCAAGGGACAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCAGGAACACCtt                                      <  2:1118204/140‑1 (MQ=255)
                                                                                                 taTGAATCCATTGTGCCTAATGCAAGGGTAAAGAATATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATTTTCCATATTGAAACTTTTACGTGTATTAATACTTAATATTGCGAGCCGTAACACCt                                       <  4:89492/137‑1 (MQ=255)
                                                                                                       tCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                               <  7:319987/140‑1 (MQ=255)
                                                                                                       tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGACCACCTTTTGTCAt                               >  5:583405/1‑140 (MQ=255)
                                                                                                       tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTc                                 <  4:1087431/138‑1 (MQ=255)
                                                                                                       tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                               >  1:787367/1‑140 (MQ=255)
                                                                                                       tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                               >  7:196688/1‑140 (MQ=255)
                                                                                                        cgaTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTTGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCa                                <  4:679645/136‑1 (MQ=255)
                                                                                                        ccATTGTTCATCTGGCAAGGGAAAAGTAAATTAG‑CCAAATTAGGTTTCTTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCTGAACACCTTTTGTCATa                              <  1:923159/140‑1 (MQ=255)
                                                                                                        ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCa                                <  4:690437/138‑1 (MQ=255)
                                                                                                         cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCATATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAATATTGCTAGCCGGAACACCTATTGTCAt                               >  3:845273/1‑138 (MQ=255)
                                                                                                               gcctCAGGCAAGGGAAAAGAAACTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTTATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  2:676551/137‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  7:405486/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGATAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  8:282146/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  4:363608/138‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  6:1089737/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  5:34087/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTACATATTGAAACTTTTTCGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  1:606312/140‑1 (MQ=255)
                                                                                                                     ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                 >  2:623314/1‑140 (MQ=255)
                                                                                                                     ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                 >  8:407954/1‑140 (MQ=255)
                                                                                                                     ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACC‑TTTGTCATAAGGGATGCGGGa                  >  4:162173/1‑138 (MQ=255)
                                                                                                                       cAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                 >  3:1002826/1‑138 (MQ=255)
                                                                                                                          gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATg              >  4:1153004/1‑138 (MQ=255)
                                                                                                                          gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg            >  1:479169/1‑140 (MQ=255)
                                                                                                                           ggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGa             >  3:20010/1‑138 (MQ=255)
                                                                                                                               aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg       >  7:1014977/1‑140 (MQ=255)
                                                                                                                               aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATCTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGTGATGCGGGATATGAGTGgcg       >  7:651801/1‑140 (MQ=255)
                                                                                                                                agaaaaTTAG‑CCAAATTAGGATTCATAGTGAAACATATGCTTTAATGAATGTTCAAAATTGAAACTTTTACGAGTATTAATACTTAAAATTGCGAGCCGGAACACCGTTTGACATAAGGGATGCGGGATATGAGTGgcgg      >  5:805302/1‑139 (MQ=255)
                                                                                                                                    aaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGATCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtttt  >  8:355476/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTT  >  NZ_CP022686/4870774‑4871046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A