Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,368,372 T→G 13.5% S37R (AGC→CGC)  ybiY ← glycyl‑radical enzyme activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,368,3720TG13.5% 339.7 / 8.5 169S37R (AGC→CGC) ybiYglycyl‑radical enzyme activating protein
Reads supporting (aligned to +/- strand):  ref base T (60/77);  new base G (25/3);  total (89/80)
Fisher's exact test for biased strand distribution p-value = 6.62e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

CGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGTTCAAA  >  NZ_CP022686/4368235‑4368504
                                                                                                                                         |                                                                                                                                       
cGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAGTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTGATAAAGCAGATCCTTCGTGCGGGCGCGGCgtt                                                                                                                                       >  2:66949/1‑140 (MQ=255)
cGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACCGCACATCCTGCGTGCGGGCGCGGCgtt                                                                                                                                       >  2:961197/1‑140 (MQ=255)
 ggggTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTc                                                                                                                                      <  1:39990/140‑1 (MQ=255)
  gggTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCCTGCGGGCGCGGCttt                                                                                                                                       >  3:989196/1‑138 (MQ=255)
         cTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCt                                                                                                                              <  7:48546/140‑1 (MQ=255)
             tCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGCTCCAGCCTGCGGGCGCGGCTTTCCGGGGTCAGacc                                                                                                                          >  2:682157/1‑139 (MQ=255)
               ccGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTACGGTGCGGTCTTATCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTAATACAACACAAGCTACGTGCGGGCGCAGGTTTACGCGttaatcca                                                                                                                          >  4:357771/1‑132 (MQ=255)
                cGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGCGTCGTGGGTCCTACAGCAGCTCCTGCGGGCGGGCGCCGCTGTCCGGGGTCTGaccc                                                                                                                         >  4:229962/1‑136 (MQ=255)
                    taaTAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTAAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCgg                                                                                                                  <  6:78071/139‑1 (MQ=255)
                           cAAACAATTCAACGCGCGGCCAATCACTTCCTCTGCGGACTGAGCGCACTGCTTACAGCCTGCCACTCAGAGTCGTGGGTCATACACCATATCCTGCGTGCGGGCGCGGCTTTCCGGTGTCTGACAC‑CAGCGTCAGCCCa                                                                                                           <  8:639001/140‑1 (MQ=255)
                              aCCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCATCTCCAGCGTGCGGGCGCCGCGTTACGGGGTCTGACCC‑CCGCCGAAGCCCACCg                                                                                                        >  7:940843/1‑140 (MQ=255)
                                cATTCAACGCGCGCTCCATCACTTCCGGGGCGGTCTTAGCGCACAGATTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                                                      <  2:1060709/140‑1 (MQ=255)
                                cATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                                                      <  7:31719/140‑1 (MQ=255)
                                cATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                                                      <  5:225927/140‑1 (MQ=255)
                                cATTCAACGCGCGCTCAATCAATTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCTGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGaa                                                                                                      <  8:414350/140‑1 (MQ=255)
                                 aTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCCCAGATTACAGCCTTCCAGACAGAGTCGTGGGTCCTTCAGCAGATCCTGCCTGCGGGCGCGGCTGAGCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAAc                                                                                                     >  5:333165/1‑140 (MQ=255)
                                 aTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGa                                                                                                       >  4:583165/1‑138 (MQ=255)
                                 aTTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAAc                                                                                                     <  5:315965/140‑1 (MQ=255)
                                    cAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACATAGTCGTGGGTCATACACCAGATCCTGCGTGCGGGCGCGGCTTTACGGGGTCTGTCAC‑CACCCGCCGCCCAGCGCACAGc                                                                                                  >  7:718310/1‑140 (MQ=255)
                                      aCGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGc                                                                                                  <  3:843471/138‑1 (MQ=255)
                                         cgcgcgCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATCCAGCAGATCCTGCGTGCGGGCGCGGCGTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                                             <  8:490501/140‑1 (MQ=255)
                                         cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCtt                                                                                               <  3:141698/138‑1 (MQ=255)
                                         cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                                             <  1:1018151/140‑1 (MQ=255)
                                         cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                                             <  2:996572/140‑1 (MQ=255)
                                         cgcgcTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATACAGACGCTCCTGCGTGCGGGCGCGGCGTTCCGGGGTCTTCCAC‑CCGCGGCAGCCCCGCGCACAGCCTTTa                                                                                             >  6:848798/1‑140 (MQ=255)
                                         cgcgcTCAATCACTTCCGGTGCGGGCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTa                                                                                             <  5:216142/140‑1 (MQ=255)
                                                aaTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATa                                                                                      <  8:995686/140‑1 (MQ=255)
                                                aaTCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGGCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATa                                                                                      <  2:1011751/140‑1 (MQ=255)
                                                    aCTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACg                                                                                    <  4:822210/138‑1 (MQ=255)
                                                    aCTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGGGGGTCATACAGCAGATCCTGCGGGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                  <  8:239430/140‑1 (MQ=255)
                                                     attCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGa                                                                                   <  3:550607/137‑1 (MQ=255)
                                                      ttCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGCATAAACCAGATACAGCGTGCCGGCGCCGCGTTCCGGGGTCTGACAC‑CCCCGGCACCCCAGCCAACAGGCATTAAAAAATACGCCCg                                                                                >  5:522100/1‑140 (MQ=255)
                                                        ccGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTAATAAAGCATCTCCTGGGTGGGGGCGCGGCTTTACGGGTGATGAAAA‑CCTCGTCAGACCCACGAAAAGCCTTTAAGAAATAAGAcactg                                                                              >  6:996682/1‑136 (MQ=255)
                                                          ggTGCGGTCTTTGCGCACAGCTTAAAGACTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CTGCGGCAGCCAAGCGAACAGCCTTTAAGAAATACGACCGTGCg                                                                            <  5:187776/140‑1 (MQ=255)
                                                          ggTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCg                                                                            <  1:708011/140‑1 (MQ=255)
                                                               ggTCTTAGCGCACAGCTTCCAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAc                                                                       <  6:932439/140‑1 (MQ=255)
                                                                gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCGTTCCGGGTTCTG‑CACACAGCGGCAGCCCCGCGCACCGCCTTTTATAAAAAAGACCGTGCGGCTa                                                                        >  3:677316/1‑138 (MQ=255)
                                                                gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCGGCGTGCGGGCGCGGCGTTCCGGGTTCTGACAC‑CAGCGGCCGCCCCGCGCACAGCCTTTAAGAAATACGCCCGGGCGGCTAc‑c                                                                     >  2:252519/1‑140 (MQ=255)
                                                                gTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGGAATACACGAGATACAGCGTGGCGGCGCGGCTTTCCGGGTTATTACAC‑CACAGTGAGCCCAGCGAACACCCTTCAAGAAATAAGACCGGTCGGAttac                                                                      >  6:68600/1‑137 (MQ=255)
                                                                     tgcgcACAGCTTACTGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGc                                                                <  1:278751/139‑1 (MQ=255)
                                                                         cacaGCTTACAGCCTTCCAGACTGAGGCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCca                                                              <  3:206385/138‑1 (MQ=255)
                                                                             gCTTACAGCCTTCCAGACAGAGTCGTGGGTGGTACAGCAGATCCTGCGTGCGGGCGCGGCGTTGCGGGTTCTGGGAC‑CAGCGGCCGCCCCGCGCACAGCCGGTAAGAAAAACGGCCGTGGGGCTTCGCGGGGcctaatgg                                                         >  2:762378/1‑134 (MQ=255)
                                                                             gCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACCGCAGATACTGCGTGCGGGCGCGGCATTACGGGTTCTGACAC‑CCGCGGCAGCCCAGCGCACAGCCGTTAAGAAATACGAACGTGCGGATAC‑CGGGGCcataaa                                                          >  4:74071/1‑135 (MQ=255)
                                                                             gCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTACGGGTTGTGACAC‑CAGCGGCAGCCCAGCGCACAGCCGTTAAGAAATACGCCCGTGCGGATAC‑CGGGGCCATC‑AAgg                                                       >  8:851949/1‑140 (MQ=255)
                                                                             gCTTACAGCCTTCCAGACAGAGTCGTGGGTCAAACAGCAGATCCTGCGGGCGGGCGCGGCTTTCCGGGTTCTGTCAC‑CACCGGCAGCCCAGCGAACCGCCTTTAAGAAATAAGACCGTGCGGATAC‑CGGGGGcatc‑aa                                                         >  4:795758/1‑137 (MQ=255)
                                                                              cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATggg                                                      <  1:16414/140‑1 (MQ=255)
                                                                              cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATggg                                                      <  1:737975/140‑1 (MQ=255)
                                                                              cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATg                                                        <  3:168568/138‑1 (MQ=255)
                                                                              cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑AAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATggg                                                      <  5:66356/140‑1 (MQ=255)
                                                                              cTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTGCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATggg                                                      <  5:66696/140‑1 (MQ=255)
                                                                               ttACAGCCTTCCAGACAGAGTCGTGGGGGATAAAGCAGATCCTGCGTGCGGGGGCGGCGTTCCGGGTTATGAAAC‑CACCCGCAGCCCCGCGAAACGCCATTTAGAAATAAGCCCGTGCGGCTAC‑CCGGGCCCTC‑AAgg                                                       >  4:206385/1‑138 (MQ=255)
                                                                                   aGCCTTCCAGACAGAGGCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAg                                                 <  1:879312/140‑1 (MQ=255)
                                                                                     ccTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTa                                               <  2:410337/140‑1 (MQ=255)
                                                                                        tCCAGACAGTGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGGTTTCCGGGGTCGGACCC‑CAGCGGCCGGCCAGCGCCCCTCCTGTAAGTAATACGACCGTGCGGATAC‑CGGGGGCGGC‑ATGGGGGGAGTAGCg                                            >  1:115098/1‑140 (MQ=255)
                                                                                        tCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCGTTACCGGTTATGACAC‑CCGCGGCCGCCCCGCGAAACGCCGTTAAGAAATACGACCGTGCGGATAA‑CGGGGCCATC‑ATGGGTCGCGGAGCg                                            >  6:924641/1‑140 (MQ=255)
                                                                                        tCCAGACAGAGTCGTGGGGCCAAAAGCACATCCTGCGTGCGGGGCCGGCGTGACGGGGTGAGCAAC‑CCCCGGCCGCCCCCCGAAAACCCGTTAAGAAAAAAGACCGGGCGGATTA‑CCGGGCCATCAATGGGT‑GAGTAgag                                            >  8:566733/1‑138 (MQ=255)
                                                                                          cagacagaGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTACGGGGTCTGACCC‑CAGCGGCCGCCCAGCGCACAGCCTTTAAGAAATACGCCCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCg                                            >  4:741811/1‑138 (MQ=255)
                                                                                          cagacagaGTCGTGGGTCATACAGCAGATCCAGCGTGCGGGCGCGGCTTTCCGGGTTCTGACCC‑CAGCGGCCGCCCAGCGAACAGCCGTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCCTC‑ATGGGTCGAGTAGCg                                            >  4:973314/1‑138 (MQ=255)
                                                                                          cagacagaGTCGTGGGTCATACAGCAGAAACAGCGTGCGGGCGCGGCTTTCCGGGTTATGACCC‑CCGCGGCCGCCCAGCGAACAGCCAGTATGAAAAACGACCGGGCGGCTAC‑CCGGGGCCTC‑AGGGGGCGAGTAGCg                                            >  4:462373/1‑138 (MQ=255)
                                                                                               agagTCGTGGGTCATACAGCAGAGCCTGCGTGCTGGCGCGGATTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑AGGGGTCGAGGAGCGTTGAata                                     <  6:203839/140‑1 (MQ=255)
                                                                                                 tgTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt                                   <  1:852021/139‑1 (MQ=255)
                                                                                                 tgTCGGGGGGCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACCC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt                                   <  7:554016/139‑1 (MQ=255)
                                                                                                 agTCGTGGGTGATACAGCAGATCCTGCGTGGGGGCGCGGCGTTGCGGGGTGTGACCC‑CAGCGGCCGCCCCGCGAACAGCCGTTTAGAAATACGACCGGGCGGATAC‑CGGGGCCCTC‑AAGGGTCGAGTTGCGGTGAATAtt                                   >  6:310093/1‑140 (MQ=255)
                                                                                                 agTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACCC‑CACCGGCAGCCCCGCGCACAGCCTTTAAGAAATACGACCGTGCGGGTAC‑CCGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt                                   >  8:534538/1‑140 (MQ=255)
                                                                                                 agTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTGCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAGACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAata                                     <  3:303068/138‑1 (MQ=255)
                                                                                                 agTCGTGGGGGCTAAACCAGATCCAGCGTGCGGGCGCGGCTTTCCGGGGTCTGGCAC‑CCGCGGCCGCCCAGCGAACAGCCTGTAAGAAATACGACCGTTGGGATAC‑CGGGGCCCTC‑ATGGGTCGAGTAGCCTTGAATAtt                                   >  8:365708/1‑140 (MQ=255)
                                                                                                 agTCGAGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CTGGGCCATC‑ATGGGTCGAGTAGCGTTGAATAtt                                   >  6:537212/1‑140 (MQ=255)
                                                                                                    cGTGGGGCATACAGCAGAACCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTg                                  <  3:1005721/138‑1 (MQ=255)
                                                                                                           cATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑Cgggg                                                                 >  5:879460/1‑101 (MQ=255)
                                                                                                           cATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑Cgggg                                                                 <  6:879460/101‑1 (MQ=255)
                                                                                                           cATAAAGCAGATACTGCGTGCGGGCGCGGCTTTACGGGGTCTGACAC‑CAGCGGCCGCCCAGCGAACATCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑CTGGGTAGAGTAGCGTTGAATATTGAAaataatt                         >  1:1093482/1‑139 (MQ=255)
                                                                                                             tACAGCAGATCCTGCGTGCGGGCGCGTCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGCCCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAATTCATAGt                       >  2:311266/1‑140 (MQ=255)
                                                                                                             tACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGCCAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGGTGAATATTGAAAATCATAGt                       >  5:953580/1‑140 (MQ=255)
                                                                                                             tACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       <  1:1006990/140‑1 (MQ=255)
                                                                                                             tACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       >  5:347912/1‑140 (MQ=255)
                                                                                                               cagcagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACTC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTg                     <  5:796386/140‑1 (MQ=255)
                                                                                                               cagcagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACTACCGTGCGGATAC‑CGTGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       >  4:831008/1‑138 (MQ=255)
                                                                                                               cagcagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       >  4:610985/1‑138 (MQ=255)
                                                                                                               cagcagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       <  4:769918/138‑1 (MQ=255)
                                                                                                               cagcagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTg                     <  2:533639/140‑1 (MQ=255)
                                                                                                               cagcagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTg                     <  2:603031/140‑1 (MQ=255)
                                                                                                               cagcagATCCTGCGGGCGGGCGCGGCTTTCCGGGTGCTGCCAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTg                     <  6:121112/140‑1 (MQ=255)
                                                                                                                  cagATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACCC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGCTAC‑CGGGGCCAGC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCct                  >  7:102233/1‑140 (MQ=255)
                                                                                                                   agATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGACCAGCCATTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTAGAATATTGAAAAACATAGTTGccac                 >  6:254740/1‑138 (MQ=255)
                                                                                                                    gATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCCGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCctct                >  6:317675/1‑140 (MQ=255)
                                                                                                                    gATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCctcg                >  2:386511/1‑139 (MQ=255)
                                                                                                                    gATCCTGCGTGCGGGCGCGGCTGTCCGGGTTCTGACCC‑CAGCGGCAGCCCCGCGAACCGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCctct                >  8:835371/1‑140 (MQ=255)
                                                                                                                       ccTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTATg             >  5:701093/1‑140 (MQ=255)
                                                                                                                         tgcgtgcgGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTg             >  3:788894/1‑138 (MQ=255)
                                                                                                                            gtgcgGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGc                                                                                                  >  3:451428/1‑52 (MQ=255)
                                                                                                                              gcgGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       >  3:457024/1‑123 (MQ=255)
                                                                                                                              gcgGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGt                       <  4:457024/123‑1 (MQ=255)
                                                                                                                              gcgGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATAGAAAATCATAGTTGCCTCTGTGTTTCg        >  3:1060384/1‑138 (MQ=255)
                                                                                                                               cgGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGtta     >  1:551030/1‑139 (MQ=255)
                                                                                                                                gggCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACATCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGTTCa    <  8:472685/140‑1 (MQ=255)
                                                                                                                                gggCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGTTCa    <  2:169763/140‑1 (MQ=255)
                                                                                                                                 ggCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGCACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGTCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGTTCaa   >  6:49668/1‑140 (MQ=255)
                                                                                                                                 ggCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                  >  7:973591/1‑63 (MQ=255)
                                                                                                                                 ggCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGAc                                                                                  <  8:973591/63‑1 (MQ=255)
                                                                                                                                  gctcgGCTTTCCGGTTTCTGACAC‑CAGCGGCAGCCAAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGTTCaaa  <  8:170761/137‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAACGCGCGCTCAATCACTTCCGGTGCGGTCTTAGCGCACAGCTTACAGCCTTCCAGACAGAGTCGTGGGTCATACAGCAGATCCTGCGTGCGGGCGCGGCTTTCCGGGTTCTGACAC‑CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATAC‑CGGGGCCATC‑ATGGGTCGAGTAGCGTTGAATATTGAAAATCATAGTTGCCTCTCTGTTTCGTTCAAA  >  NZ_CP022686/4368235‑4368504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A