Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,605,579 T→G 15.4% T56P (ACG→CCG)  CIW80_RS23010 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,605,5790TG15.4% 194.3 / 3.5 120T56P (ACG→CCG) CIW80_RS23010hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (4/92);  new base G (9/12);  total (16/104)
Fisher's exact test for biased strand distribution p-value = 1.66e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

AAAAACCGCCAGCATGACTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACTTAAT  >  NZ_CP022686/4605442‑4605717
                                                                                                                                         |                                                                                                                                              
aaaaaCCGCCAGCATGACTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCt                                                                                                                                              <  8:527304/140‑1 (MQ=255)
     ccGCCAGCATGACTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGc                                                                                                                                           <  3:1028968/138‑1 (MQ=255)
     ccGCCAGCATGACTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGa                                                                                                                                         <  6:810736/140‑1 (MQ=255)
           gCATGACTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGc                                                                                                                                   <  2:376661/140‑1 (MQ=255)
                aCTTCGTTAACCACTCCCTGCTAAAAAAGCAGAAACTGGTACGTTATCAACACAAAACTGCAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTggcg                                                                                                                              <  8:529905/140‑1 (MQ=255)
                aCTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTgg                                                                                                                                <  4:916858/138‑1 (MQ=255)
                aCTGCGTAAACCACTACCGGGAAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTggcg                                                                                                                              <  8:387232/140‑1 (MQ=255)
                    cGTTAACCACTCCCGGGTAAAACAGCAGTAACTGGAACGTTTTCAACAGAAAACTGGAGACACACAATACAATCCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTggcgcagg                                                                                                                          <  6:327297/140‑1 (MQ=255)
                    cGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTggcgcagg                                                                                                                          <  2:670940/140‑1 (MQ=255)
                    cGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTggcgcagg                                                                                                                          <  2:39417/140‑1 (MQ=255)
                      ttAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGGGTGGGGTCGGGGCTggcgcaaggg                                                                                                                        >  8:249590/1‑136 (MQ=255)
                        aaCCACTACCGGGTAAAACAGCAGTAACTGGGACGGTTTCAACAGCACACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGGCTGGCGACGGGGCTggcgcaggc‑g                                                                                                                       <  3:24862/138‑1 (MQ=255)
                        aaCCACAACAGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTggcgcaggc‑g                                                                                                                       <  4:81753/138‑1 (MQ=255)
                          ccccTACCGCGTAAAAACTCAGTAACTGGTTCGGTTTCAACCTTAAACTGGTTTCTCACAATACAATGCCCACAACAGGACCGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAAt                                                                                                                   <  6:901482/137‑1 (MQ=255)
                               cccGGGTAAACGAGCAGTAACGGGGACGTTATCAAGAGCAACCTGGAGACACACAATACAATGCCCACAACACCAACGATTTTTGTGACGACCTGGAGCGGCCCCGGCTGGAGACGGGGCTGGCGCAGGC‑GCAATAAAAg                                                                                                              <  6:293931/139‑1 (MQ=255)
                                ccGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGCGGGGGCCGGGGCGGGGGCCGGG‑GGCATaaaaaa                                                                                                             >  7:263091/1‑138 (MQ=255)
                                 cGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAg                                                                                                              <  3:99679/138‑1 (MQ=255)
                                   ggTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGCTTGGGCCCGGGCGGGCGCCGGG‑GCAATAAAAACaca                                                                                                          >  7:271261/1‑138 (MQ=255)
                                    gTAAAACAGCAGGAACTGGTACGTTTTCAACAGAAAACTGGAGACACCCAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACa                                                                                                         <  6:562121/140‑1 (MQ=255)
                                     tAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCTCTGGGGACGGGGCTGGCGCACGC‑GCAATAAAATCAAc                                                                                                          >  4:990859/1‑138 (MQ=255)
                                     tAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCTCGCGCGCCCGGGGTGGCGCACGG‑GCCAAAAAAACaaaac                                                                                                        >  2:655837/1‑137 (MQ=255)
                                          cagcagTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAgcg‑c                                                                                                    <  4:436685/138‑1 (MQ=255)
                                          cagcagTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCa                                                                                                  <  8:744622/140‑1 (MQ=255)
                                          cagcagTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGCGTGCGCCGGGGCTGGCGCCCGC‑GCAATAAAAGCACCAgccc                                                                                                     >  4:665933/1‑136 (MQ=255)
                                             cagTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCACAGGGGGCGGGGCGGGGGCACGC‑GGCCTAAAAACAACACCG‑cccaca                                                                                               >  5:586266/1‑136 (MQ=255)
                                              tgTAAGTGGTTAGTTTTCAACATTAAACTGTAGACACACAATACATTGCCCACCACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATc                                                                                                <  3:113788/137‑1 (MQ=255)
                                              tgTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAg                                                                                              <  2:204588/139‑1 (MQ=255)
                                              agTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCAGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAg                                                                                              <  6:487460/140‑1 (MQ=255)
                                                  aCTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGATTGGGGCGGGGCGGGGGCCGGC‑GGAATAAAAGCAAACGCG‑CCCTccggcgc                                                                                          >  6:272305/1‑134 (MQ=255)
                                                   cTGGTACGTTTTCAAGAGAACACTGGTCACACTCAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTTGCGCCGCCCCGGCTGGCGACGGGGCGGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑a                                                                                        <  5:441789/140‑1 (MQ=255)
                                                   cTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑a                                                                                        <  8:377602/140‑1 (MQ=255)
                                                   cTGGGACCTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACCACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCgg                                                                                           <  3:647487/138‑1 (MQ=255)
                                                        aCGTTTTCCAGAGAAATGTGGAGACACACAATACAATGCCCACAACAGCAAGGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGTCGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGc                                                                                   <  5:737793/140‑1 (MQ=255)
                                                        aCGTTTTCACCAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAAATAGTTTTGTGACGCCCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCACTAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGc                                                                                   <  1:617744/140‑1 (MQ=255)
                                                         cGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCTCTGGCGCCGGGGCTGGCGCAGGC‑GCAATAAAAGCCACCGCG‑CCCTCAGCGGC‑cacac                                                                                    >  3:471070/1‑137 (MQ=255)
                                                         cGTTTGAAACAGAAAACTGGAGACACACAATACAATCCCCACAACAGCAACGATTTTTGTGTCGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCTCAGGC‑TCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGcc                                                                                  <  2:651652/140‑1 (MQ=255)
                                                            tttCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGt                                                                               <  7:80366/140‑1 (MQ=255)
                                                            tgtCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGTTTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCTCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGt                                                                               <  5:463657/138‑1 (MQ=255)
                                                             ttCTACAGAAAACTGGAGACACACAATACAATGACCATAACAGCCACGAGTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCTACAGCG‑CCATCAGAGGC‑AACAGCCAg                                                                                <  3:327957/138‑1 (MQ=255)
                                                             ttCAACAGAAAACTGGAGACACACAATCCAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTg                                                                              <  8:474000/140‑1 (MQ=255)
                                                             ttCAACAGAAAACTGGAGACACACAATACAATGCCCACAACATCAAGTATTTTTGTGACGACCTGGAGCGGCGCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTg                                                                              <  7:629990/140‑1 (MQ=255)
                                                             ttCAACAGAAAACTGGAGACAAACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑ACATCAGCGGC‑AACAGCCAGTg                                                                              <  7:73779/140‑1 (MQ=255)
                                                               cAACAGAAAACTGGCGACACACAATACAATGCCCACACCCGCAACGATTTTTGGGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCa                                                                            <  1:290076/140‑1 (MQ=255)
                                                               cAACAGAAAACTGGAGACACACACTACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTg                                                                              <  4:402490/138‑1 (MQ=255)
                                                               cAACAGAAAACTGGAGACACACAATACACTGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCa                                                                            <  6:834006/140‑1 (MQ=255)
                                                               cAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCACCGATTTTTGTGACGACCTGGAGCGGCCCCGGCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGGGCa                                                                            <  2:941883/140‑1 (MQ=255)
                                                               cAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCa                                                                            <  5:951145/140‑1 (MQ=255)
                                                               cAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCa                                                                            <  2:691312/140‑1 (MQ=255)
                                                               cAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGAGTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTg                                                                              <  3:1070595/138‑1 (MQ=255)
                                                                 ccAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCCGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  1:1040211/139‑1 (MQ=255)
                                                                 aCAGAAACCTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  6:184364/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGCGACACACCATCCAATGCCCCCAACCGCAACGATTTTTGTGACGACCTGGCGCGGCCCCGGCTGGCGACGGGGCTGGCGCAGGC‑GCAATAACAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  1:72047/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAATACAATGCCCACACCAGCAACGATTTGTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  7:507161/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  8:284947/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  5:502526/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  7:288686/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  1:317861/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGGGACGACCGGGAGCGGCCCCGGCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  5:1000270/140‑1 (MQ=255)
                                                                 aCAGAAAACTGGAGACACACAAAACAATGCCCACAAAAGCAACTATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAgg                                                                          <  1:289017/140‑1 (MQ=255)
                                                                      aaaCTGGAGACACACAATCCAATGCCCACAACAGCAACGATTTTTGTCACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGtt                                                                     <  2:922180/140‑1 (MQ=255)
                                                                            gagaCACACAATCCAATGCCCCCAACAGCAACGATTTTTGGGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CAc                                                              <  1:643212/140‑1 (MQ=255)
                                                                            gagaCACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑c                                                                <  3:425677/138‑1 (MQ=255)
                                                                            gagaCACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CAc                                                              <  7:522948/140‑1 (MQ=255)
                                                                            gagaCACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CAc                                                              <  2:1000675/140‑1 (MQ=255)
                                                                            gagaCACACAATACAAAGCCCACAACAGCAACGATTTTGGAGACGACCTGGAGCGGCCCCGGCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑c                                                                <  3:326583/138‑1 (MQ=255)
                                                                              gaCACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGCTGGGGCCGGGGGTGGCGCCCGC‑GCCAAAAAACCAACCCCG‑CCATCAGCGGC‑AACAACCAGTGCAGGCGGTTATgacc                                                               >  4:404176/1‑135 (MQ=255)
                                                                                      atacaatGCCCACAACAGCAACGCTTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTa                                                      <  3:482618/138‑1 (MQ=255)
                                                                                      atacaatGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTa                                                      <  4:534246/138‑1 (MQ=255)
                                                                                          aatCCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGa                                                <  1:555162/140‑1 (MQ=255)
                                                                                            tGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCTCTGGGGCCGGGGCTTGCGACGGC‑GCCATAAAATCAAAACCC‑CCATAACCGGC‑AAAAACCAAGGACGGCAGTTATT‑CACCAAACCAAGCCAAAtt                                              >  6:556481/1‑140 (MQ=255)
                                                                                                ccccacaGCAACTATTTTTTTGTCGACCGGGAGCGGCCCCGTTTGGCGACGGGGCTGGCGCATGC‑GCTATAAAAGCAACAGCG‑GCATCAGCTGC‑AACAGCCAGTGCAGGATGTTATG‑AACCAAACCTAGCCATATTaaaa                                          <  8:814544/136‑1 (MQ=255)
                                                                                                cacaACAGCAACGATTTTTGTGACTACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCATGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTaa                                            <  4:860920/138‑1 (MQ=255)
                                                                                                 acaacaGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGCGGGGGCCCGGGGGGGCGCCCGCGGCAATAAAA‑CAACAGCC‑CCCAAAGCGGCAAACACCCCG‑GGAGGGGGTTTTG‑AACCCAACCAAGCCAGaattaa                                           >  4:998969/1‑133 (MQ=255)
                                                                                                 acaacaGCAACGATTTTTGTGACGACCTGGAGCGGCCCCCGCAGGGGCCCGGGGAGGCGCCAGC‑GCAAAAAAATCAACAACGCCCAACA‑CGGC‑CACACCCCCGGCACGCTTGTTTG‑CACCCAACCAAACCCGATTaaa                                           >  4:99108/1‑138 (MQ=255)
                                                                                                         aaCGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGa                                 <  2:1058287/140‑1 (MQ=255)
                                                                                                         aaCGATTTTTGCGACGACCTGGAGCGGGCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTCGCCAGATTAAAAAAGGCCAGa                                 <  1:125628/140‑1 (MQ=255)
                                                                                                          aCGATTTTTGTGACGACCTGGAGCGGCCCCCCAGGTCGCCCGGGCTGGCGCACGC‑GCCAAAAAAACAACAACG‑CCAACAGCCGC‑AACACCCACGGCAGGAGGAGGTGACACCAAACC‑AGCCACATGAAAAAATGcgcc                                  >  4:294188/1‑135 (MQ=255)
                                                                                                           cGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGa                                 <  4:1109383/138‑1 (MQ=255)
                                                                                                           agATTTTTGTGACGACCTGGAGATGACCCGTATGTCGAAGTGGCTGGCGCAGGC‑GCAATAAAAGAAACAGAG‑CCATAAGCGGG‑AACAGCCAGTGCAGGCTGTTATT‑CACCAAACATAGCCTTATTAAAAAATGACAGACg                               <  8:274498/139‑1 (MQ=255)
                                                                                                             ttgtttGTGACGACCTGGTGCGGCCCCGTCGGGCGACGGGGCTGGCGCATGC‑GAAATAAAAGCAACAGCG‑CCATGAGCGGC‑AACAGCCAGTGCTGGCTGTTATG‑CACCATACCTAGCCAGATTAAAAAAGGCCAGACg                               <  4:907026/135‑1 (MQ=255)
                                                                                                             attattGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGag                             <  8:876323/140‑1 (MQ=255)
                                                                                                             aTTTTTGTGACGCCCTGGAGCGGCCCCGTCTCGCGACGGGGCTGGCGCAGGC‑GCAAAAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGag                             <  2:684070/140‑1 (MQ=255)
                                                                                                             aTTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCTTGAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACg                               <  4:970502/138‑1 (MQ=255)
                                                                                                             aTTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGag                             <  1:645433/140‑1 (MQ=255)
                                                                                                             aTTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCGGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGag                             <  6:897024/140‑1 (MQ=255)
                                                                                                             aTTTTTGTGACGACCTGGAGCGGCCCCGGCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGag                             <  6:1057127/140‑1 (MQ=255)
                                                                                                             aTTTTTGTGACGACCTGGAGCGGCCCCGGCTGGCGACGGGGCGGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGag                             <  2:876872/140‑1 (MQ=255)
                                                                                                                tttGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGagcag                          <  7:1007046/140‑1 (MQ=255)
                                                                                                                tttGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGagc                            <  4:215359/138‑1 (MQ=255)
                                                                                                                        gacCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATc                  <  8:691879/140‑1 (MQ=255)
                                                                                                                            gggAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCATACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCg                <  4:1110575/137‑1 (MQ=255)
                                                                                                                                gCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGc          <  8:118182/140‑1 (MQ=255)
                                                                                                                                    ccccGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCaa        <  3:404176/138‑1 (MQ=255)
                                                                                                                                    ccccGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCaa        <  3:121708/138‑1 (MQ=255)
                                                                                                                                    ccccGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACt      <  7:745133/140‑1 (MQ=255)
                                                                                                                                    ccccGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACt      <  7:312759/140‑1 (MQ=255)
                                                                                                                                      ccGTCTGGCGACGGGGCTGGCGCATGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCATCAACt      <  4:1040787/138‑1 (MQ=255)
                                                                                                                                      ccGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACTTa    <  6:855163/140‑1 (MQ=255)
                                                                                                                                      ccGGCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACTTa    <  6:21367/140‑1 (MQ=255)
                                                                                                                                        gTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGTC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACTTAat  <  8:76372/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                              
AAAAACCGCCAGCATGACTGCGTTAACCACTACCGGGTAAAACAGCAGTAACTGGTACGTTTTCAACAGAAAACTGGAGACACACAATACAATGCCCACAACAGCAACGATTTTTGTGACGACCTGGAGCGGCCCCGTCTGGCGACGGGGCTGGCGCAGGC‑GCAATAAAAGCAACAGCG‑CCATCAGCGGC‑AACAGCCAGTGCAGGCTGTTATG‑CACCAAACCTAGCCAGATTAAAAAAGGCCAGACGAGCAGCAATAATCCGGTCAGCAACTTAAT  >  NZ_CP022686/4605442‑4605717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A