Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,712,763 A→C 16.4% A52A (GCT→GCG)  yceB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,712,7630AC16.4% 149.6 / 2.1 90A52A (GCT→GCG) yceBlipoprotein
Reads supporting (aligned to +/- strand):  ref base A (59/13);  new base C (2/15);  total (62/28)
Fisher's exact test for biased strand distribution p-value = 9.29e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAA  >  NZ_CP022686/4712624‑4712902
                                                                                                                                              |                                                                                                                                           
tCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTACCC‑TTATTCGGCTCGTCGCGACCAATTT‑GGCTGG‑TCAGGGTTGTCAGAACAATATGGGCGTCa                                                                                                                                             >  8:24395/1‑140 (MQ=255)
tCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTAATGTCCAGATTGGCGTCACCCGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAAATT‑TGCGGG‑TCAGGTTTGTCAGAACAATATGGGCGTCa                                                                                                                                             >  5:115923/1‑140 (MQ=255)
      tctgTTTCTTGGTGGTTTTCGGGCTACCGAACAGGGTGTTAATGTCCATTTGGGCGGATCTGGTTTGGGTAACC‑TTATTGGGCTCTTCGCTACAATTTG‑GGCTGG‑TCTGGTTTGTCATAAAAATATGGGCGTCAGCCACg                                                                                                                                       <  2:925107/137‑1 (MQ=255)
         gTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGc                                                                                                                                      >  4:314714/1‑138 (MQ=255)
         gTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTAAGAACAATATGGGCGTCAGCCACGc                                                                                                                                      >  4:309325/1‑138 (MQ=255)
          tttCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTACCC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACACTATGGGCGTCCGCCACGcc                                                                                                                                     >  4:928254/1‑138 (MQ=255)
          tttCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCgg                                                                                                                                   >  8:437890/1‑140 (MQ=255)
              aTGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTaa                                                                                                                               >  1:940047/1‑140 (MQ=255)
                    gCTTTCTGGCTACCGCACAGGGAGTTGATGTCCAGATTGGCGTCTCCGGTTAGGGTATGC‑TTTTTCGGCTGGTCGCGGCCCATTT‑GGGTGG‑TCATGTTTTTGAGAACAAGATGGGCGGGAGCCACGCCGGGTAAACCGat                                                                                                                         >  8:552893/1‑140 (MQ=255)
                    gCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAAAATGGGCGTCAGCCACGCCCGGTAAACCGat                                                                                                                         >  7:1054769/1‑140 (MQ=255)
                               aCCTAACAGGTTGTTAATGTCCATTTTGGCGCGTCGGGTTTGGTTAACT‑TTATTCGGCTCTTCGCCACAATTTG‑GGCTGG‑TATGGTTTGCCATAAAAATATGGGGGTCCCCCACGCCGGGTAAACCGATATCTTTTgaga                                                                                                              <  2:607483/140‑1 (MQ=255)
                                ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  8:246193/1‑140 (MQ=255)
                                ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TAATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGCACAATATGGGCGTCAGCCACGCCGGGTAATCCGATAACTTTTGAGaa                                                                                                             >  7:896146/1‑140 (MQ=255)
                                  gAACAGGGAGTTCGTGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTATTCGCGACGAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  4:199969/1‑138 (MQ=255)
                                       gggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGTGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattat                                                                                                        >  4:6411/1‑138 (MQ=255)
                                        ggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                                                                                     >  8:353462/1‑140 (MQ=255)
                                        ggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                                                                                     >  8:3525/1‑140 (MQ=255)
                                          aGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                                                                                     >  3:682967/1‑138 (MQ=255)
                                                 gTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCTACCAATTT‑GGCTGG‑TAAGCTTATTCACAACAATAATGGCGTCAGCCACGACGGGAAAACCCATATCTATTGATAAATTATTATCTTTCGc                                                                                              >  3:560257/1‑138 (MQ=255)
                                                 gTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGc                                                                                              >  3:560266/1‑138 (MQ=255)
                                                    cAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGCCCAATTT‑GGCTGG‑GCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCCGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCg                                                                                         >  5:1051693/1‑140 (MQ=255)
                                                    cAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGCCCAATAT‑GGCTGG‑TCAGGTTAGTCAGAACAATAAGGGCGAAAGCCACGCCCGGTAAACCGCTCTCTTTTGAGAAATTATTATGTTTAGCAAGCg                                                                                         >  6:171118/1‑140 (MQ=255)
                                                      gATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGCCCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                       >  2:469372/1‑140 (MQ=255)
                                                      gATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCg                                                                                         >  3:657191/1‑138 (MQ=255)
                                                      gATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                       >  8:44739/1‑140 (MQ=255)
                                                      gATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAAAATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTAGCAAGCGAc                                                                                       >  6:431474/1‑140 (MQ=255)
                                                       aTTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑ACAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAcg                                                                                      >  2:681103/1‑139 (MQ=255)
                                                        ttGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                       >  3:950693/1‑138 (MQ=255)
                                                         tGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACt                                                                                      >  4:465794/1‑138 (MQ=255)
                                                              cctcCGGTTCGGGAAACC‑TTATTCGGCTCTTCGCCCCAAATTT‑GGCTGG‑GCAGGTTTGTCAGAAAAATATGGGGTGCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTa                                                                                 <  4:405915/137‑1 (MQ=255)
                                                                      ttggttAACC‑TTTTTCGGTGCTGCGCTACATATTT‑GGCTGG‑TTTGTTTTTTCCTAACAATAGGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTg                                                                         <  3:804238/133‑1 (MQ=255)
                                                                      ttggttAACA‑TTTTGCGGCTCTTCCCCACCATTTG‑GGCTGG‑TCTGGTTTGTCCAAAAATTTGGGGCGCCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                                                       <  7:3525/135‑1 (MQ=255)
                                                                      tcggtaaaCA‑TTTTTCGTCGCTGCGCTACAAATTG‑GGCTGG‑GCTGGTTTGTAACAAAAATAGGGGCGTCCCCCACGCCGGGTTAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                                                       <  7:354993/134‑1 (MQ=255)
                                                                       ttggTAACCATTATTCGGCTATTCGCTACAAATTG‑GGCTGT‑GCATGTTTGTCACAAAAATCTGGGCTGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTg                                                                         <  3:809486/136‑1 (MQ=255)
                                                                         gggaaCC‑TTATCCGGAACGTCCCTACAAATTT‑GCCTTC‑GCAGGTTTGGCACAAATATTGGGGGTTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc                                                                      <  3:154702/135‑1 (MQ=255)
                                                                         gcaaccc‑TTATGGGGCCCTCCGCTCCAAATTG‑GCCGGG‑TCCGGTTGGCCAGAACACTAGGGGCGACCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                    <  6:108667/135‑1 (MQ=255)
                                                                           tAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                    >  4:764412/1‑138 (MQ=255)
                                                                            tatcgttATTCGGCGTTGCGAGACCAATTT‑GCAGGG‑GCAGGTTTGTCAGAACAATTTGGGGGTCCGCCACGCCGTGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                  <  6:853038/135‑1 (MQ=255)
                                                                             ccc‑TTTTGCGGCGCGTCGCGACCTATTC‑GGCTGG‑TCAGGTTTGCACGAAAAATATGGGTGGCAGCCACGCCGGGTAAACCGATATCTTTTGAGATATTCTTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                <  6:272051/139‑1 (MQ=255)
                                                                             aCC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGGGAt                                                                >  7:273578/1‑140 (MQ=255)
                                                                              ccttttttCGGGTCGTCGGGACTAATTT‑GGCTGG‑TCAGTTTTGGCTTAACAATTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTGTTGATCGGTg                                                                  <  4:892144/132‑1 (MQ=255)
                                                                              cc‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               >  6:925624/1‑140 (MQ=255)
                                                                              cc‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTAGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               >  7:1089355/1‑140 (MQ=255)
                                                                              cc‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGCGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGGAAGCGACTGGTTAAGTTCTTGTTGGGTGATg                                                               >  1:759056/1‑140 (MQ=255)
                                                                              cc‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAAAATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAGTTCTTGTTCGGTGATg                                                               >  6:925626/1‑140 (MQ=255)
                                                                              atattATTCGCCGCTTGGCGAAAATTTT‑GGGTGG‑ACTGTTTTTAAATAAAATTAGGGGCGCCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                  <  3:314714/135‑1 (MQ=255)
                                                                              acattATGCGCCTGTACGCGAACTATTT‑GGCGGG‑TCTGTTGTGTCACAAAAATTGGGGCGGCCTCCACGCCGGGTAAACCGATATCTTTTGAGAAATAATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                <  5:306046/137‑1 (MQ=255)
                                                                               ctttATGCGGCGTTTCGCCACCATTTG‑GGCTGG‑TCAGTTTTTTCAGAAAAATTTGGGGGTGAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               <  8:409751/138‑1 (MQ=255)
                                                                                 ttATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               >  4:156906/1‑138 (MQ=255)
                                                                                  tATTCGGCTCTTCGCGACAAATTT‑GGCGGG‑TCAGGTTTGACAGAAAAATATGGGCGGCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtg                                                            <  2:51880/140‑1 (MQ=255)
                                                                                   aTTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtgt                                                           <  8:334096/140‑1 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtgtt                                                          >  2:790894/1‑139 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtgtt                                                          >  1:510653/1‑139 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTAGGTGATGGTGTa                                                          >  5:676062/1‑140 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGGAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  7:420483/1‑140 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGCTGG‑GCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTAGCAAGCGACTGGTTAATTTCTTGTTCGGTGAGGGTGTa                                                          >  6:1078102/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGGGGCGGCCCCGCCGGTTAACCCGTTTAGTTTTTATAATTATTTTTTTTTCGCAACCGCGTGGTTAATTTGTTGTTGGGGGATGGTGTATTggg                                                     >  2:177958/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGTTGATGGTGTATTggg                                                     >  1:758578/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGAAAACCCAGATCTTATGAGAAATTAATATGTTTCGCAAGAGACTGGTTAATTTCTTGTTCGGTGATGgtgctgtctc                                                     >  7:952085/1‑133 (MQ=255)
                                                                                           ctTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  3:177201/1‑138 (MQ=255)
                                                                                             tCGCTAACAATTT‑TGCTGT‑TCTGTTTTTTCACAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                                                 <  6:652109/140‑1 (MQ=255)
                                                                                             gctccaCCATTTT‑GCCTGG‑GCAGGTTTGACACAAAAATATGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                                                 <  2:921532/135‑1 (MQ=255)
                                                                                              cgcgACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGt                                                >  5:389211/1‑140 (MQ=255)
                                                                                               cgctacAATTT‑GGGGGGTTCTGGTTTGTCACAAAAATTTGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGt                                                <  6:421865/135‑1 (MQ=255)
                                                                                                   acAATTTGGGCTGT‑TCTTGTTTTTAATAAAATTATGGGGGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAt                                            <  5:1039456/139‑1 (MQ=255)
                                                                                                     atttt‑GGTTGG‑TCTGGTTGGGCATAACAATTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAtt                                           <  4:682967/136‑1 (MQ=255)
                                                                                                           ggttGG‑TCCGGTTTGACCTAAAAATCGGGGCGCCCCCCACGCCGGGTAAACCGATCTCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCa                                    <  6:115923/137‑1 (MQ=255)
                                                                                                            gCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAATCCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCaa                                   >  6:223799/1‑140 (MQ=255)
                                                                                                                g‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               >  8:588360/1‑140 (MQ=255)
                                                                                                                g‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               >  1:496079/1‑140 (MQ=255)
                                                                                                                 gtaagttttGACATAAAATTAGGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               <  1:844568/134‑1 (MQ=255)
                                                                                                                   cAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               >  4:62184/1‑138 (MQ=255)
                                                                                                                           tCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     >  7:750338/1‑140 (MQ=255)
                                                                                                                           tCAGAACAATATGGGCGTCAGCCACGCCGGCTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTCATGGTGTATTCGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     >  8:1063370/1‑140 (MQ=255)
                                                                                                                           tCAGAACAATATGGGCGTCAGCCAATCCGGGTAAACCGAAATCTATTGAGAAATTATTATGTTTAGCAAGCGACTGGTTAATTTCATGTTCTGTGATGGTGTATTGGGTGATTTGATTACCGCCAACGAGCAGGCCTCTg                     >  7:750337/1‑140 (MQ=255)
                                                                                                                           acccaaaaaTATGGGCGGCCGCCACGCCGTGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     <  2:848071/133‑1 (MQ=255)
                                                                                                                             aGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     >  4:966519/1‑138 (MQ=255)
                                                                                                                              aaaaaaTATGGGCGGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACg                  <  7:246193/136‑1 (MQ=255)
                                                                                                                                aaaaTTTGGGCTCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACg                  <  3:520490/136‑1 (MQ=255)
                                                                                                                                    tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCTGGCCTCTGACGATCact            >  5:11608/1‑138 (MQ=255)
                                                                                                                                    tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  5:794239/1‑140 (MQ=255)
                                                                                                                                    tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  1:30913/1‑140 (MQ=255)
                                                                                                                                         gCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc       >  1:306788/1‑140 (MQ=255)
                                                                                                                                           gTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAg                            <  3:1120483/117‑1 (MQ=255)
                                                                                                                                           gTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAg                            >  4:1120483/1‑117 (MQ=255)
                                                                                                                                            tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  1:1097600/1‑140 (MQ=255)
                                                                                                                                            tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  5:944518/1‑140 (MQ=255)
                                                                                                                                            tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  5:889064/1‑140 (MQ=255)
                                                                                                                                            tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  1:615993/1‑140 (MQ=255)
                                                                                                                                             cAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTTTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACTCGCAGGCCACTGACGATCAATGCAGCgg     >  3:876900/1‑138 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGTTGAGTTGATTACATCCAACGAGCAGGCCACTGACTATCAATGCAGAGGCaa  >  1:1073362/1‑140 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGc                          <  8:1017741/116‑1 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGc                          >  7:1017741/1‑116 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  3:861399/1‑138 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  3:362525/1‑138 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  8:1049535/1‑140 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  7:996192/1‑140 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  8:775215/1‑140 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGGGTATTGGGTGAGTTGATTACAGCCCACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  5:119051/1‑140 (MQ=255)
                                                                                                                                              aGCCACGCCGGGTAAACCGATATCTTTAGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGGGCGGTGATGGTGTATTGGGTGAGTTGCTTACAGCCAACGAGCAGGCCACTGACCAACAATGCAGCGGCaa  >  6:1036745/1‑140 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGCTGG‑TCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAA  >  NZ_CP022686/4712624‑4712902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A