Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F224 I0 R1	1457	284.0	9290224	95.7%	8890744	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,365,283	G→T	100%	E271* (GAG→TAG)	ycjM →	alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,365,283	0	G	T	98.2%	900.0 / 5.8	279	E271* (GAG→TAG)	ycjM	alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base G (2/3); new base T (144/128); total (148/131)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 281 total aligned reads displayed.

GGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA  >  CP009273/1365136‑1365428

ggTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtag                                                                                                                                                 <  1:3079802/150‑1 (MQ=255)

 gTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga                                                                                                                                                >  1:2628155/1‑150 (MQ=255)

     tGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCaa                                                                                                                                            >  2:1566094/1‑150 (MQ=255)

     tGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCaa                                                                                                                                            >  1:337712/1‑150 (MQ=255)

     tGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCaa                                                                                                                                            >  2:2856958/1‑150 (MQ=255)

     tGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCTAGACCaa                                                                                                                                            >  1:3257942/1‑150 (MQ=255)

      gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                           <  1:3660260/150‑1 (MQ=255)

        gTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGt                                                                                                                                         <  2:1472613/150‑1 (MQ=255)

         tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt                                                                                                                                        <  2:697514/150‑1 (MQ=255)

            gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                     <  1:4157640/150‑1 (MQ=255)

            gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                     <  1:2425640/150‑1 (MQ=255)

             tCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGc                                                                                                                                    >  2:3190629/1‑150 (MQ=255)

             tCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGc                                                                                                                                    <  1:1409845/150‑1 (MQ=255)

              ccGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATTACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCa                                                                                                                                   >  1:47595/1‑150 (MQ=255)

                gCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATa                                                                                                                                 >  1:189361/1‑150 (MQ=255)

                 ccTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa                                                                                                                                <  2:2228240/150‑1 (MQ=255)

                    ggATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGa                                                                                                                             >  2:2484025/1‑150 (MQ=255)

                     gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac                                                                                                                            <  2:3886279/150‑1 (MQ=255)

                          cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAt                                                                                                                       <  2:2400339/150‑1 (MQ=255)

                           gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAtt                                                                                                                      <  1:2822337/150‑1 (MQ=255)

                              ggCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCt                                                                                                                   <  1:1588698/150‑1 (MQ=255)

                                cTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTa                                                                                                                 <  2:2975898/150‑1 (MQ=255)

                                 tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAc                                                                                                                >  1:3954068/1‑150 (MQ=255)

                                   tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACtt                                                                                                              >  2:915156/1‑150 (MQ=255)

                                   tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACtt                                                                                                              <  1:4547741/150‑1 (MQ=255)

                                   tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACtt                                                                                                              <  2:1622950/150‑1 (MQ=255)

                                   tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAAAACATTGCTTACtt                                                                                                              >  2:3028716/1‑150 (MQ=255)

                                     tgtgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTg                                                                                                            <  1:3894927/150‑1 (MQ=255)

                                       tgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgc                                                                                                          <  2:319132/150‑1 (MQ=255)

                                           aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                      >  1:2154166/1‑150 (MQ=255)

                                            agagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAgg                                                                                                     >  1:1033767/1‑150 (MQ=255)

                                                ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGGTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGAt                                                                                                 <  2:3649850/150‑1 (MQ=255)

                                                 cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATg                                                                                                <  1:4483106/150‑1 (MQ=255)

                                                  gggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGa                                                                                               >  1:1554802/1‑150 (MQ=255)

                                                  gggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGa                                                                                               <  1:424/150‑1 (MQ=255)

                                                   ggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAc                                                                                              <  2:3597390/150‑1 (MQ=255)

                                                   ggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAc                                                                                              >  1:4584343/1‑150 (MQ=255)

                                                     aaCAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                            >  1:3864457/1‑150 (MQ=255)

                                                        aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                         >  2:4407767/1‑150 (MQ=255)

                                                         aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGca                                                                                        >  2:259486/1‑150 (MQ=255)

                                                         aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGca                                                                                        <  2:3416859/150‑1 (MQ=255)

                                                         aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGca                                                                                        <  2:3416955/150‑1 (MQ=255)

                                                            tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACat                                                                                     >  1:1032253/1‑150 (MQ=255)

                                                            tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACat                                                                                     <  1:11327/150‑1 (MQ=255)

                                                              catccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGGTTACTTTGGCGCAGGCGATGACGAAGCCCatat                                                                                   >  2:507938/1‑150 (MQ=255)

                                                              catccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACatat                                                                                   >  2:3278677/1‑150 (MQ=255)

                                                               atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATg                                                                                  >  2:2677620/1‑150 (MQ=255)

                                                               atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATg                                                                                  <  1:2756161/150‑1 (MQ=255)

                                                                tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATgg                                                                                 <  2:4025886/150‑1 (MQ=255)

                                                                  catcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtg                                                                               >  2:2824108/1‑150 (MQ=255)

                                                                      tGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAcc                                                                           <  1:3146844/150‑1 (MQ=255)

                                                                      tGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAcc                                                                           >  2:1359641/1‑150 (MQ=255)

                                                                        gAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAg                                                                         >  2:4311960/1‑150 (MQ=255)

                                                                          aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                       >  2:210193/1‑150 (MQ=255)

                                                                          aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                       >  2:3900352/1‑150 (MQ=255)

                                                                                acttCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCt                                                                 <  2:721504/147‑1 (MQ=255)

                                                                                acaTCTTATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATAATTAACTAGACCAATTTTCCGCATAAAGACAACATTTCTTACATTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCt                                                                 >  2:2432269/1‑150 (MQ=255)

                                                                                acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCt                                                                 >  1:1460766/1‑150 (MQ=255)

                                                                                acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCt                                                                 <  1:1314570/150‑1 (MQ=255)

                                                                                acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCt                                                                 <  2:1972737/150‑1 (MQ=255)

                                                                                  aTCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTCTCGCTga                                                               >  1:1657533/1‑149 (MQ=255)

                                                                                    cTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAATTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccg                                                             >  1:2319915/1‑150 (MQ=255)

                                                                                     tGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                            >  2:4229138/1‑150 (MQ=255)

                                                                                     tGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                            >  1:1775247/1‑150 (MQ=255)

                                                                                          aTCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACATATTGTGTAACAGTTCTCGCTGCCGCCACTg                                                       >  1:1162644/1‑150 (MQ=255)

                                                                                            cAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGt                                                     >  1:2691838/1‑150 (MQ=255)

                                                                                            cAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGt                                                     <  2:1236058/150‑1 (MQ=255)

                                                                                               aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgct                                                  >  1:3733379/1‑150 (MQ=255)

                                                                                                   ttACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCAt                                              <  2:4110034/150‑1 (MQ=255)

                                                                                                   ttACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCAt                                              <  2:1104350/150‑1 (MQ=255)

                                                                                                    tACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATg                                             <  1:4101636/150‑1 (MQ=255)

                                                                                                          cGATTATTGATAACGTTGCGCCAGGTACAGNGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGc                                       >  1:4285032/1‑150 (MQ=255)

                                                                                                           gATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCa                                      >  1:64557/1‑150 (MQ=255)

                                                                                                            attattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaa                                     >  2:586661/1‑150 (MQ=255)

                                                                                                             ttattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaa                                    >  1:176814/1‑150 (MQ=255)

                                                                                                               attGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaaa                                  >  2:428638/1‑150 (MQ=255)

                                                                                                               attGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaaa                                  >  1:909575/1‑150 (MQ=255)

                                                                                                                  gATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                               <  2:1957015/150‑1 (MQ=255)

                                                                                                                    tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGaa                             <  1:3321900/150‑1 (MQ=255)

                                                                                                                     aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                            >  2:2453518/1‑150 (MQ=255)

                                                                                                                     aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                            <  2:1247213/150‑1 (MQ=255)

                                                                                                                     aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                            <  2:3150450/150‑1 (MQ=255)

                                                                                                                      aCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                           <  2:3849869/150‑1 (MQ=255)

                                                                                                                          tGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGa                       >  2:2048910/1‑150 (MQ=255)

                                                                                                                              ccAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcg                   >  2:3742000/1‑150 (MQ=255)

                                                                                                                              ccAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcg                   <  2:3547302/150‑1 (MQ=255)

                                                                                                                              ccAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcg                   <  2:1029395/150‑1 (MQ=255)

                                                                                                                                aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATACAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                 <  2:337712/150‑1 (MQ=255)

                                                                                                                                aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                 >  1:356289/1‑150 (MQ=255)

                                                                                                                                 ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt                >  1:2873427/1‑150 (MQ=255)

                                                                                                                                  gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt               <  2:1497853/150‑1 (MQ=255)

                                                                                                                                   tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg              <  1:2824079/150‑1 (MQ=255)

                                                                                                                                   tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg              >  2:4397691/1‑150 (MQ=255)

                                                                                                                                    aCAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtgt             >  2:3231787/1‑150 (MQ=255)

                                                                                                                                    aCAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtgt             <  2:1136328/150‑1 (MQ=255)

                                                                                                                                         gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTg        <  1:1519981/150‑1 (MQ=255)

                                                                                                                                         gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTg        <  1:1425692/150‑1 (MQ=255)

                                                                                                                                          aTCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg       >  1:344101/1‑150 (MQ=255)

                                                                                                                                              ttACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcgc   <  2:4584343/150‑1 (MQ=255)

                                                                                                                                              ttACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcgc   <  2:108611/150‑1 (MQ=255)

GGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA  >  CP009273/1365136‑1365428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365135‑1365432

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                      <  SRR19973072.2563751/150‑1 (MQ=60)

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                      <  SRR19973072.2563206/150‑1 (MQ=60)

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                      <  SRR19973072.1915654/150‑1 (MQ=60)

 GGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAG                                                                                                                                                     <  SRR19973072.3086302/150‑1 (MQ=60)

  GTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGA                                                                                                                                                    >  SRR19973072.2633802/1‑150 (MQ=60)

      TGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAA                                                                                                                                                >  SRR19973072.338467/1‑150 (MQ=60)

      TGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCTAGACCAA                                                                                                                                                >  SRR19973072.3264859/1‑150 (MQ=60)

      TGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAA                                                                                                                                                >  SRR19973072.2344992/1‑150 (MQ=60)

       GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTGCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                               <  SRR19973072.1576574/150‑1 (MQ=60)

       GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                               <  SRR19973072.3668536/150‑1 (MQ=60)

       GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                               <  SRR19973072.3667970/150‑1 (MQ=60)

       GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                               <  SRR19973072.4261983/150‑1 (MQ=60)

          TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                            <  SRR19973072.2950512/150‑1 (MQ=60)

           ATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTC                                                                                                                                           >  SRR19973072.553989/1‑150 (MQ=60)

             GTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCG                                                                                                                                         <  SRR19973072.4166356/150‑1 (MQ=60)

             GTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCG                                                                                                                                         <  SRR19973072.2430896/150‑1 (MQ=60)

             GTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCG                                                                                                                                         <  SRR19973072.2473307/150‑1 (MQ=60)

              TCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGC                                                                                                                                        <  SRR19973072.1412915/150‑1 (MQ=60)

               CCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATTACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                       >  SRR19973072.47721/1‑150 (MQ=60)

               CCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                       >  SRR19973072.47588/1‑150 (MQ=60)

                 GCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATA                                                                                                                                     >  SRR19973072.189842/1‑150 (MQ=60)

                 GCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATA                                                                                                                                     <  SRR19973072.3397230/150‑1 (MQ=60)

                 GCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATA                                                                                                                                     <  SRR19973072.3929757/150‑1 (MQ=60)

                    TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                  >  SRR19973072.2100139/1‑150 (MQ=60)

                    TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                  >  SRR19973072.291577/1‑150 (MQ=60)

                    TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                  <  SRR19973072.1236850/150‑1 (MQ=60)

                    TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                  >  SRR19973072.3969782/1‑150 (MQ=60)

                           CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAT                                                                                                                           >  SRR19973072.1896169/1‑150 (MQ=60)

                            GTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATT                                                                                                                          <  SRR19973072.2828386/150‑1 (MQ=60)

                            GTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATT                                                                                                                          <  SRR19973072.2828391/150‑1 (MQ=60)

                             TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                         >  SRR19973072.1501048/1‑150 (MQ=60)

                               GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCT                                                                                                                       <  SRR19973072.1592110/150‑1 (MQ=60)

                               GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCT                                                                                                                       >  SRR19973072.4338240/1‑150 (MQ=60)

                                 CTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTA                                                                                                                     >  SRR19973072.3370538/1‑150 (MQ=60)

                                 CTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTA                                                                                                                     >  SRR19973072.3370430/1‑150 (MQ=60)

                                 CTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTA                                                                                                                     >  SRR19973072.2208866/1‑150 (MQ=60)

                                  TTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAC                                                                                                                    >  SRR19973072.3962332/1‑150 (MQ=60)

                                    TATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTT                                                                                                                  <  SRR19973072.16406/150‑1 (MQ=60)

                                    TATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTT                                                                                                                  <  SRR19973072.4557329/150‑1 (MQ=60)

                                      TGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTG                                                                                                                <  SRR19973072.3903018/150‑1 (MQ=60)

                                      TGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTG                                                                                                                <  SRR19973072.3903036/150‑1 (MQ=60)

                                         GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCG                                                                                                             >  SRR19973072.2639298/1‑150 (MQ=60)

                                         GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCG                                                                                                             >  SRR19973072.2639258/1‑150 (MQ=60)

                                          GAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGC                                                                                                            >  SRR19973072.1847178/1‑150 (MQ=60)

                                           AAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCA                                                                                                           <  SRR19973072.4075680/150‑1 (MQ=60)

                                            AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                          >  SRR19973072.2158854/1‑150 (MQ=60)

                                             AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGG                                                                                                         >  SRR19973072.1036003/1‑150 (MQ=60)

                                             AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGG                                                                                                         >  SRR19973072.1035977/1‑150 (MQ=60)

                                                  CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    <  SRR19973072.4492568/150‑1 (MQ=60)

                                                  CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    <  SRR19973072.4492700/150‑1 (MQ=60)

                                                  CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    <  SRR19973072.349850/150‑1 (MQ=60)

                                                  CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    >  SRR19973072.1140431/1‑150 (MQ=60)

                                                   GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGA                                                                                                   <  SRR19973072.427/150‑1 (MQ=60)

                                                   GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGA                                                                                                   >  SRR19973072.946068/1‑150 (MQ=60)

                                                   GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGA                                                                                                   >  SRR19973072.1558105/1‑150 (MQ=60)

                                                   GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACCTTGCTTACATTGGCGCAGGCGATTA                                                                                                   >  SRR19973072.3912836/1‑150 (MQ=60)

                                                    GGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAC                                                                                                  >  SRR19973072.4593978/1‑150 (MQ=60)

                                                    GGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTTCTTACTTTGGCGCAGGCGATGAC                                                                                                  >  SRR19973072.108880/1‑150 (MQ=60)

                                                      AACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGA                                                                                                >  SRR19973072.3872495/1‑150 (MQ=60)

                                                        CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAG                                                                                              >  SRR19973072.1552556/1‑150 (MQ=60)

                                                         AAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGC                                                                                             >  SRR19973072.3818681/1‑150 (MQ=60)

                                                         AAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGC                                                                                             >  SRR19973072.284196/1‑150 (MQ=60)

                                                             TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                         >  SRR19973072.1034485/1‑150 (MQ=60)

                                                             TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                         <  SRR19973072.4555332/150‑1 (MQ=60)

                                                             TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                         <  SRR19973072.11367/150‑1 (MQ=60)

                                                             TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                         >  SRR19973072.2102268/1‑150 (MQ=60)

                                                                ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                      <  SRR19973072.2762054/150‑1 (MQ=60)

                                                                    ATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGT                                                                                  >  SRR19973072.815215/1‑150 (MQ=60)

                                                                     TCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTA                                                                                 >  SRR19973072.1454201/1‑150 (MQ=60)

                                                                       TGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACC                                                                               >  SRR19973072.1441640/1‑150 (MQ=60)

                                                                       TGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACC                                                                               <  SRR19973072.3153486/150‑1 (MQ=60)

                                                                       TGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACC                                                                               >  SRR19973072.4236442/1‑150 (MQ=60)

                                                                              AACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTC                                                                        >  SRR19973072.3166710/1‑150 (MQ=60)

                                                                              AACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTC                                                                        >  SRR19973072.3166911/1‑150 (MQ=60)

                                                                               ACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCG                                                                       <  SRR19973072.4286910/150‑1 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTAGCT                                                                     >  SRR19973072.130757/1‑150 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     <  SRR19973072.274591/150‑1 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     <  SRR19973072.1095388/150‑1 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     <  SRR19973072.1317441/150‑1 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     <  SRR19973072.274596/150‑1 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     >  SRR19973072.1463904/1‑150 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     <  SRR19973072.1317433/150‑1 (MQ=60)

                                                                                 ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                     <  SRR19973072.1589760/150‑1 (MQ=60)

                                                                                  CATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTACCAGTTCTCGCTG                                                                    >  SRR19973072.93850/1‑150 (MQ=60)

                                                                                   ATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTCTCGCTGA                                                                   >  SRR19973072.1661164/1‑150 (MQ=60)

                                                                                     CTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAATTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCG                                                                 >  SRR19973072.2324879/1‑150 (MQ=60)

                                                                                     CTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCG                                                                 >  SRR19973072.1145353/1‑150 (MQ=60)

                                                                                      TGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGC                                                                >  SRR19973072.1779133/1‑150 (MQ=60)

                                                                                       GATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCC                                                               <  SRR19973072.3107484/150‑1 (MQ=60)

                                                                                       GATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCC                                                               <  SRR19973072.3107418/150‑1 (MQ=60)

                                                                                       GATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCC                                                               >  SRR19973072.690514/1‑150 (MQ=60)

                                                                                         TTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCAC                                                             >  SRR19973072.4174997/1‑150 (MQ=60)

                                                                                          TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                            >  SRR19973072.277030/1‑150 (MQ=60)

                                                                                          TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                            >  SRR19973072.277054/1‑150 (MQ=60)

                                                                                           ATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACATATTGTGTAACAGTTCTCGCTGCCGCCACTG                                                           >  SRR19973072.1165071/1‑150 (MQ=60)

                                                                                           ATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTG                                                           <  SRR19973072.4593047/150‑1 (MQ=60)

                                                                                             CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGT                                                         >  SRR19973072.2697566/1‑150 (MQ=60)

                                                                                                ACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCT                                                      >  SRR19973072.3741236/1‑150 (MQ=60)

                                                                                                 CTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTG                                                     >  SRR19973072.1739003/1‑150 (MQ=60)

                                                                                                   GTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACCTATGGTGTACCAGTTCTAGCAGCCGCCACTGGTGCTGCA                                                   >  SRR19973072.4562817/1‑150 (MQ=60)

                                                                                                     TACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATG                                                 <  SRR19973072.1478650/150‑1 (MQ=60)

                                                                                                     TACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATG                                                 <  SRR19973072.1775217/150‑1 (MQ=60)

                                                                                                     TACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATG                                                 <  SRR19973072.4110250/150‑1 (MQ=60)

                                                                                                     TACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATG                                                 <  SRR19973072.4110256/150‑1 (MQ=60)

                                                                                                           CGATTATTGATAACGTTGCGCCAGGTACAGNGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGC                                           >  SRR19973072.4293954/1‑150 (MQ=60)

                                                                                                            GATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCA                                          >  SRR19973072.64730/1‑150 (MQ=60)

                                                                                                            GATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGATCTCGCTGCCGCCACTGGTGCTGCATGAGGTGCA                                          >  SRR19973072.2382892/1‑150 (MQ=60)

                                                                                                              TTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAA                                        >  SRR19973072.177333/1‑150 (MQ=60)

                                                                                                              TTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAA                                        >  SRR19973072.177263/1‑150 (MQ=60)

                                                                                                                ATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAA                                      >  SRR19973072.911542/1‑150 (MQ=60)

                                                                                                                 TTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAC                                     <  SRR19973072.3548872/150‑1 (MQ=60)

                                                                                                                     TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                                 <  SRR19973072.3649759/150‑1 (MQ=60)

                                                                                                                     TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                                 <  SRR19973072.3328798/150‑1 (MQ=60)

                                                                                                                     TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                                 <  SRR19973072.3328954/150‑1 (MQ=60)

                                                                                                                      AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                                <  SRR19973072.594707/150‑1 (MQ=60)

                                                                                                                        CGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGT                              >  SRR19973072.2447916/1‑150 (MQ=60)

                                                                                                                                CAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGC                      >  SRR19973072.3598779/1‑150 (MQ=60)

                                                                                                                                 AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT                     >  SRR19973072.357080/1‑150 (MQ=60)

                                                                                                                                  GGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTT                    >  SRR19973072.2879586/1‑150 (MQ=60)

                                                                                                                                    TACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTG                  <  SRR19973072.2830130/150‑1 (MQ=60)

                                                                                                                                    TACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTG                  <  SRR19973072.2830159/150‑1 (MQ=60)

                                                                                                                                        cgatctATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCG              <  SRR19973072.3893105/144‑1 (MQ=60)

                                                                                                                                        GTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCG              <  SRR19973072.1355855/150‑1 (MQ=60)

                                                                                                                                          GATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTG            <  SRR19973072.1523210/150‑1 (MQ=60)

                                                                                                                                          GATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTG            <  SRR19973072.1428780/150‑1 (MQ=60)

                                                                                                                                          GATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTG            <  SRR19973072.1523278/150‑1 (MQ=60)

                                                                                                                                           ATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGG           >  SRR19973072.344872/1‑150 (MQ=60)

                                                                                                                                           ATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGG           >  SRR19973072.344635/1‑150 (MQ=60)

                                                                                                                                            TCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGG          >  SRR19973072.1416537/1‑150 (MQ=60)

                                                                                                                                                TACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA      >  SRR19973072.3754598/1‑150 (MQ=60)

                                                                                                                                                TACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA      <  SRR19973072.1770780/150‑1 (MQ=60)

                                                                                                                                                TACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA      >  SRR19973072.4333442/1‑150 (MQ=60)

                                                                                                                                                    TAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  SRR19973072.1624989/1‑150 (MQ=60)

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365135‑1365432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`