Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F309 I0 R1	1697	197.2	6627756	95.7%	6342762	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,365,283	G→T	100%	E271* (GAG→TAG)	ycjM →	alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,365,283	0	G	T	99.3%	558.6 / ‑5.7	190	E271* (GAG→TAG)	ycjM	alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base G (1/0); major base T (93/91); minor base A (5/0); total (99/91)
Fisher's exact test for biased strand distribution p-value = 6.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365139‑1365426

gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga                                                                                                                                               <  2:2722740/148‑1 (MQ=255)

   gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa                                                                                                                                            <  1:2451731/148‑1 (MQ=255)

     gTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                          <  2:937952/148‑1 (MQ=255)

      tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg                                                                                                                                         <  1:2750604/148‑1 (MQ=255)

        tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt                                                                                                                                       <  2:205633/148‑1 (MQ=255)

               cTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATa                                                                                                                                <  1:2807653/148‑1 (MQ=255)

               cTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATa                                                                                                                                <  1:2807639/148‑1 (MQ=255)

                tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa                                                                                                                               <  1:1886705/148‑1 (MQ=255)

                tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa                                                                                                                               <  1:2729614/148‑1 (MQ=255)

                  gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTTTTGATAAAGTTGCGCCACGTACAGTGATCATTAACAATAACAAAGTTACGCATAAAg                                                                                                                             >  1:2222816/1‑148 (MQ=255)

                  gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACTTTTCGCCACGTACAGTTATAATTAACTAGAACAAAGTTACGCAAAAAg                                                                                                                             >  1:561189/1‑148 (MQ=255)

                     gCCGTTGGCTTTATGTGGAGAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac‑a                                                                                                                         <  2:3286614/148‑1 (MQ=255)

                     gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac‑a                                                                                                                         <  1:265819/148‑1 (MQ=255)

                       cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac‑aac                                                                                                                       <  1:1033449/148‑1 (MQ=255)

                         ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAt                                                                                                                     >  2:2667706/1‑148 (MQ=255)

                         ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAt                                                                                                                     <  2:2745854/148‑1 (MQ=255)

                         ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAt                                                                                                                     >  2:2489937/1‑148 (MQ=255)

                            gCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGc                                                                                                                  >  2:1353227/1‑148 (MQ=255)

                             cTTTATGTGGAAAGAGCCGGGAACAAGCTGAATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTAATTATAAAGTTTCCCCAGGTACAGTGATCAATACCTAGACCAATTTTACGCATAAATAA‑AAAATTTCt                                                                                                                 >  2:1691119/1‑148 (MQ=255)

                              tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCtt                                                                                                                <  2:3171498/148‑1 (MQ=255)

                               ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTa                                                                                                               <  1:2650133/148‑1 (MQ=255)

                               ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTa                                                                                                               <  1:2650804/148‑1 (MQ=255)

                                tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTAc                                                                                                              <  1:2285602/148‑1 (MQ=255)

                                       aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGGACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTGACTTTGgcg                                                                                                       >  1:910642/1‑148 (MQ=255)

                                           agCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAgg                                                                                                   <  2:127127/148‑1 (MQ=255)

                                           agCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAgg                                                                                                   <  2:126172/148‑1 (MQ=255)

                                             ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCg                                                                                                 >  2:834534/1‑148 (MQ=255)

                                              cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGCTCATTACCTAGACCAATTTTCCGCATAAATAC‑AACATTGCTTAATTTGGCGCAGGCGa                                                                                                >  1:2980780/1‑148 (MQ=255)

                                              cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGa                                                                                                <  2:2994515/148‑1 (MQ=255)

                                                      aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                        >  2:3180249/1‑148 (MQ=255)

                                                      aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                        >  1:2726096/1‑148 (MQ=255)

                                                      aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                        <  2:2001532/148‑1 (MQ=255)

                                                        cTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGca                                                                                      <  1:2592348/148‑1 (MQ=255)

                                                          gCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGcaca                                                                                    <  1:3166428/148‑1 (MQ=255)

                                                            atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata                                                                                  <  1:2124054/148‑1 (MQ=255)

                                                            atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata                                                                                  <  1:2124131/148‑1 (MQ=255)

                                                            atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata                                                                                  <  2:1454857/148‑1 (MQ=255)

                                                              ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATg                                                                                <  2:1627520/148‑1 (MQ=255)

                                                                 tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTACGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATtgtg                                                                             >  2:1021350/1‑148 (MQ=255)

                                                                  cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATATCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtgt                                                                            <  2:406372/148‑1 (MQ=255)

                                                                  cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtgt                                                                            <  1:1407682/148‑1 (MQ=255)

                                                                    ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACGTTGGCGCCGGCGATGACGAAGCACAGATGGTGTAc                                                                          >  2:2811329/1‑148 (MQ=255)

                                                                     gAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAcc                                                                         >  1:3025398/1‑148 (MQ=255)

                                                                       aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTACCAg                                                                       >  2:507234/1‑148 (MQ=255)

                                                                        aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCACGTACAGTGATCATTACCTATACCAAAGTTCCCCAAAAATAC‑AACAATTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                      >  1:2921795/1‑148 (MQ=255)

                                                                        aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                      <  1:906469/148‑1 (MQ=255)

                                                                        aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGAAAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCCGt                                                                      >  2:1475729/1‑148 (MQ=255)

                                                                        aaaaCACATCTGAGTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                      <  2:1999329/148‑1 (MQ=255)

                                                                         aaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                     <  1:355728/148‑1 (MQ=255)

                                                                          aaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtc                                                                    <  2:1849747/148‑1 (MQ=255)

                                                                           acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTTCCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtct                                                                   >  2:2581641/1‑148 (MQ=255)

                                                                           acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtct                                                                   <  2:2837166/148‑1 (MQ=255)

                                                                            cacaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGGTCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACCTATGGTGTACCAGTtctc                                                                  >  2:3084137/1‑148 (MQ=255)

                                                                            cacaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtctc                                                                  >  2:1548977/1‑148 (MQ=255)

                                                                            cacaTCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTAAACTGATCATTAACAAGAACAATGTTCCCCATAAAGACAAACATT‑CTTACTTTTGCCCAGGCGATTACGAAGCACATATGGTGTACCAGttata                                                                  >  1:677271/1‑145 (MQ=255)

                                                                              caTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGc                                                                <  2:1744359/148‑1 (MQ=255)

                                                                                tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg                                                              <  2:401253/148‑1 (MQ=255)

                                                                                tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg                                                              <  2:1285255/148‑1 (MQ=255)

                                                                                tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGAC‑AACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg                                                              >  2:1496464/1‑148 (MQ=255)

                                                                                      tATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATTGTGTACCAGTTCTCGCTGCCGCCa                                                        >  1:527870/1‑148 (MQ=255)

                                                                                      tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATGATTACCTAGACCAATGTTCCGCATAAAGAA‑AACAATGCTTACTTTGGCGCAGGCGCTGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCa                                                        >  1:2404661/1‑148 (MQ=255)

                                                                                      tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCa                                                        >  2:1086377/1‑148 (MQ=255)

                                                                                         cAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAA‑AACATTTCTTAATTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTg                                                     >  1:2678201/1‑148 (MQ=255)

                                                                                            aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGTCGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtg                                                  >  2:1218597/1‑148 (MQ=255)

                                                                                            aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtg                                                  <  1:1967298/148‑1 (MQ=255)

                                                                                              tGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgct                                                >  2:2438882/1‑148 (MQ=255)

                                                                                                   cGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATg                                           <  2:2846606/148‑1 (MQ=255)

                                                                                                      tCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCgg                                        >  2:1818204/1‑148 (MQ=255)

                                                                                                      tCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCgg                                        >  2:2666365/1‑148 (MQ=255)

                                                                                                            attGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaa                                  >  2:678050/1‑148 (MQ=255)

                                                                                                                aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              >  2:170235/1‑148 (MQ=255)

                                                                                                                aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              >  2:2113784/1‑148 (MQ=255)

                                                                                                                 tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                             >  2:230297/1‑148 (MQ=255)

                                                                                                                 tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                             >  2:2606280/1‑148 (MQ=255)

                                                                                                                 tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                             >  1:1059568/1‑148 (MQ=255)

                                                                                                                  aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGa                            >  2:463898/1‑148 (MQ=255)

                                                                                                                     gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                         <  2:561189/148‑1 (MQ=255)

                                                                                                                     gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                         <  1:62635/148‑1 (MQ=255)

                                                                                                                     gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                         <  2:2222816/148‑1 (MQ=255)

                                                                                                                         cgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGa                     <  1:3013230/148‑1 (MQ=255)

                                                                                                                             aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcg                 <  2:264033/148‑1 (MQ=255)

                                                                                                                              ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcgc                >  2:2793006/1‑148 (MQ=255)

                                                                                                                              ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcgc                >  2:1640071/1‑148 (MQ=255)

                                                                                                                               gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               >  2:2067134/1‑148 (MQ=255)

                                                                                                                               gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               <  1:1310237/148‑1 (MQ=255)

                                                                                                                               gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCACAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               <  1:2489942/148‑1 (MQ=255)

                                                                                                                                tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt              <  2:2981725/148‑1 (MQ=255)

                                                                                                                                tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt              <  1:2941450/148‑1 (MQ=255)

                                                                                                                                 aCAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt             >  2:2295261/1‑148 (MQ=255)

                                                                                                                                      gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  1:101592/1‑148 (MQ=255)

                                                                                                                                      gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        <  1:2112949/148‑1 (MQ=255)

                                                                                                                                      gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  2:2197800/1‑148 (MQ=255)

                                                                                                                                      gATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  2:2101527/1‑148 (MQ=255)

                                                                                                                                       aTCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGt       >  2:733953/1‑148 (MQ=255)

                                                                                                                                         cATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg     >  1:287153/1‑148 (MQ=255)

                                                                                                                                         cATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg     >  1:3061664/1‑148 (MQ=255)

                                                                                                                                          aTTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTggg    >  1:1124035/1‑148 (MQ=255)

                                                                                                                                            tACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  2:3124102/1‑148 (MQ=255)

                                                                                                                                            tACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  2:711715/1‑148 (MQ=255)

                                                                                                                                            tACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGNACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  1:1677893/1‑148 (MQ=255)

GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365139‑1365426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365142‑1365432

GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                                <  SRR19973070.2467769/150‑1 (MQ=60)

  GTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGT                                                                                                                                              <  SRR19973070.1731834/150‑1 (MQ=60)

   TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                             <  SRR19973070.2768273/150‑1 (MQ=60)

   TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                             <  SRR19973070.2768244/150‑1 (MQ=60)

        CCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                        >  SRR19973070.531526/1‑150 (MQ=60)

            CTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAA                                                                                                                                    <  SRR19973070.2825802/150‑1 (MQ=60)

            CTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAA                                                                                                                                    <  SRR19973070.2825788/150‑1 (MQ=60)

            CTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAA                                                                                                                                    <  SRR19973070.2825708/150‑1 (MQ=60)

             TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                   <  SRR19973070.2747210/150‑1 (MQ=60)

             TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                   <  SRR19973070.1899513/150‑1 (MQ=60)

               GATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC                                                                                                                                 <  SRR19973070.2216308/150‑1 (MQ=60)

                  GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AAC                                                                                                                             <  SRR19973070.267581/150‑1 (MQ=60)

                  GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AAC                                                                                                                             <  SRR19973070.267567/150‑1 (MQ=60)

                    CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAT                                                                                                                           <  SRR19973070.1040758/150‑1 (MQ=60)

                     GTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATAC‑AACATT                                                                                                                          >  SRR19973070.1062177/1‑150 (MQ=60)

                     GTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATT                                                                                                                          <  SRR19973070.2441362/150‑1 (MQ=60)

                      TTGGCTTTATGTGGAAAGAGCCGTGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTG                                                                                                                         <  SRR19973070.2534121/150‑1 (MQ=60)

                            TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACT                                                                                                                   <  SRR19973070.2668052/150‑1 (MQ=60)

                            TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACT                                                                                                                   <  SRR19973070.2667530/150‑1 (MQ=60)

                            TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACT                                                                                                                   <  SRR19973070.2667378/150‑1 (MQ=60)

                             TATGTGGAAAGAGCCGGTAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTT                                                                                                                  <  SRR19973070.2683807/150‑1 (MQ=60)

                             TATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTT                                                                                                                  <  SRR19973070.2300804/150‑1 (MQ=60)

                             TATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTT                                                                                                                  <  SRR19973070.2990163/150‑1 (MQ=60)

                                  GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGGACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTGACTTTGGCG                                                                                                             >  SRR19973070.917112/1‑150 (MQ=60)

                                     AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAATGCTTACTTTGGCGCAG                                                                                                          >  SRR19973070.52651/1‑150 (MQ=60)

                                         GCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGCTCATTACCTAGACCAATTTTCCGCATAAATAC‑AACATTGCTTAATTTGGCGCAGGCGA                                                                                                      >  SRR19973070.2999908/1‑150 (MQ=60)

                                          CCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACAATGCTTACTTTTGCGCAGGCGAT                                                                                                     >  SRR19973070.2844602/1‑150 (MQ=60)

                                          CCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGGACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGGTTAATTTGGCGCAGGCGAT                                                                                                     >  SRR19973070.915727/1‑150 (MQ=60)

                                           CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    <  SRR19973070.2743824/150‑1 (MQ=60)

                                           CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    <  SRR19973070.1081378/150‑1 (MQ=60)

                                               AACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGA                                                                                                >  SRR19973070.3000857/1‑150 (MQ=60)

                                                 CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAG                                                                                              >  SRR19973070.2743681/1‑150 (MQ=60)

                                                 CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGGGATCAATACCAAGACCAAAGTTCCGCAAAAAGAC‑AACATTTCGTACGTTGGCGCAGGCGATtacgacc                                                                                              >  SRR19973070.2743662/1‑143 (MQ=60)

                                                     CTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACA                                                                                          <  SRR19973070.2609288/150‑1 (MQ=60)

                                                       GCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATA                                                                                        <  SRR19973070.3186919/150‑1 (MQ=60)

                                                       GCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATA                                                                                        <  SRR19973070.3186904/150‑1 (MQ=60)

                                                         ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                      <  SRR19973070.2138327/150‑1 (MQ=60)

                                                         ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                      <  SRR19973070.2138267/150‑1 (MQ=60)

                                                         ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                      <  SRR19973070.2138345/150‑1 (MQ=60)

                                                         ATACATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                      <  SRR19973070.2138066/150‑1 (MQ=60)

                                                               CTGGAAAAAACACATCTGGTTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTAAAGTGATCATTAACTATACCAAAGTTCCGCATAAATAA‑AACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTAA                                                                                >  SRR19973070.307604/1‑150 (MQ=60)

                                                               CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAC                                                                                <  SRR19973070.1417250/150‑1 (MQ=60)

                                                                TGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACC                                                                               >  SRR19973070.3044670/1‑150 (MQ=60)

                                                                   AAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCACGTACAGTGATCATTACCTATACCAAAGTTCCCCAAAAATAC‑AACAATTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGT                                                                            >  SRR19973070.2940661/1‑150 (MQ=60)

                                                                   AAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGAC‑AACATTTCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGT                                                                            >  SRR19973070.2940611/1‑150 (MQ=60)

                                                                    AAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATAC‑AACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTT                                                                           >  SRR19973070.3311361/1‑150 (MQ=60)

                                                                    AAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAACAATGTTCCGCATAAATAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTT                                                                           >  SRR19973070.1664228/1‑150 (MQ=60)

                                                                     AAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTC                                                                          <  SRR19973070.912904/150‑1 (MQ=60)

                                                                      AAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCT                                                                         <  SRR19973070.358313/150‑1 (MQ=60)

                                                                       AACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAAAGTTGCGCCAGGTAAAGTGATAATTACCAAGAACAATTTTACGCATAAAGAC‑AACAATGCTTAATTTTTCGCAGGCGATGACGAACCACAAATGGTGTAacacttcta                                                                        >  SRR19973070.682213/1‑141 (MQ=60)

                                                                       AACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTAAACTGATCATTAACAAGAACAATGTTCCCCATAAAGACAAACATT‑CTTACTTTTGCCCAGGCGATTACGAAGCACATATGGTGTACCAGTTata                                                                        >  SRR19973070.682100/1‑147 (MQ=60)

                                                                              CTGATTATCAAACTGTTACGGTCGATTTTTTATAAAGTTTCGCCAGGTACAGTGATACTTACCAAGACCAAAGTTCCGCATAAAGAC‑AAAATTGCTTAATTTGGGGCAGGCGATGACGAAGCACAAATTGTGTACCAGTTatagcggccc                                                                 >  SRR19973070.2341191/1‑140 (MQ=60)

                                                                                GATTATCAAACTGTTACGGTCGATTTTTGATAACCTTGCGCCAGGTAACGTGATAATTACCAAGAACAAAGTTCCGCCAAAAGAC‑AAAAATTCTTACTTTGGCGCAGGCGCTTAAGAAGCACAAATGGTGTACCActtctagcttccccc                                                               >  SRR19973070.121829/1‑135 (MQ=41)

                                                                                 ATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATTGTGTACCAGTTCTCGCTGCCGCCA                                                              >  SRR19973070.531435/1‑150 (MQ=60)

                                                                                 ATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATGATTACCTAGACCAATGTTCCGCATAAAGAA‑AACAATGCTTACTTTGGCGCAGGCGCTGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCA                                                              >  SRR19973070.2420520/1‑150 (MQ=60)

                                                                                  TTATCAAACTGTTACGGTCGATTATTTTTAAAGTTTCGCCAGGTACAGTGTTCATTAACTAGACCAATGTTCCGCATTAAGAC‑AAAATTGCTTACTTTTGCGCAGGGGGTGTCGAAGCACATATGGTGTTCCAGTTGTCGCTGGCGCCAC                                                             >  SRR19973070.2348983/1‑150 (MQ=60)

                                                                                   TATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATAATTACCGATACCAAATTTACGCATAAAGAC‑AACATTGCGTACTTTTGCGCAGGCGATGACCAAGCACATATGGTGTAACAGTTCTCGCTGCCGCCACA                                                            >  SRR19973070.1731983/1‑150 (MQ=60)

                                                                                    ATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAA‑AACATTTCTTAATTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTG                                                           >  SRR19973070.2695588/1‑150 (MQ=60)

                                                                                         ACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCT                                                      <  SRR19973070.1980583/150‑1 (MQ=60)

                                                                                         ACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCT                                                      <  SRR19973070.1980975/150‑1 (MQ=60)

                                                                                                CGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATTACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCG                                               >  SRR19973070.1351248/1‑150 (MQ=60)

                                                                                                     GATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCA                                          >  SRR19973070.1317517/1‑150 (MQ=60)

                                                                                                            GATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAG                                   >  SRR19973070.1067002/1‑150 (MQ=60)

                                                                                                            GATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAG                                   <  SRR19973070.3033322/150‑1 (MQ=60)

                                                                                                               AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                                >  SRR19973070.1936039/1‑150 (MQ=60)

                                                                                                                  GTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTT                             <  SRR19973070.63338/150‑1 (MQ=60)

                                                                                                                  GTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTT                             <  SRR19973070.63413/150‑1 (MQ=60)

                                                                                                                      CGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGG                         <  SRR19973070.3032457/150‑1 (MQ=60)

                                                                                                                        CCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCG                       >  SRR19973070.2640224/1‑150 (MQ=60)

                                                                                                                           GGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTT                    >  SRR19973070.2942871/1‑150 (MQ=60)

                                                                                                                            GTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTT                   <  SRR19973070.1319330/150‑1 (MQ=60)

                                                                                                                            GTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCACAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTT                   <  SRR19973070.2506102/150‑1 (MQ=60)

                                                                                                                            GTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCACAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTT                   <  SRR19973070.2506107/150‑1 (MQ=60)

                                                                                                                             TACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTG                  <  SRR19973070.2960404/150‑1 (MQ=60)

                                                                                                                                 GTGATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCG              >  SRR19973070.102562/1‑150 (MQ=60)

                                                                                                                                   GATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTG            <  SRR19973070.2127116/150‑1 (MQ=60)

                                                                                                                                    ATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGG           >  SRR19973070.3081266/1‑150 (MQ=60)

                                                                                                                                    ATCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGG           >  SRR19973070.289081/1‑150 (MQ=60)

                                                                                                                                     TCATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGG          >  SRR19973070.1131752/1‑150 (MQ=60)

                                                                                                                                       ATTACCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGNACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG        >  SRR19973070.1689379/1‑150 (MQ=60)

                                                                                                                                           CCTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA    >  SRR19973070.330108/1‑150 (MQ=60)

                                                                                                                                            CTAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAA   >  SRR19973070.3173891/1‑150 (MQ=60)

                                                                                                                                             TAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  SRR19973070.385016/1‑150 (MQ=60)

GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGAC‑AACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365142‑1365432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`