Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,365,283 G→T 100% E271* (GAG→TAG)  ycjM → alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,365,2830GT96.7% 685.3 / 12.2 239E271* (GAG→TAG) ycjMalpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base T (125/98);  total (137/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 239 total aligned reads displayed.

GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA  >  CP009273/1365139‑1365428
                                                                                                                                                |                                                                                                                                                 
gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAcc                                                                                                                                              <  1:303278/150‑1 (MQ=255)
 cTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa                                                                                                                                             <  2:747225/150‑1 (MQ=255)
    aGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg                                                                                                                                          <  1:335340/150‑1 (MQ=255)
      tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt                                                                                                                                        <  1:3969930/150‑1 (MQ=255)
        tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTcc                                                                                                                                      <  2:1203514/150‑1 (MQ=255)
        tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATGTTcc                                                                                                                                      >  1:993807/1‑150 (MQ=255)
         gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                     >  2:71933/1‑150 (MQ=255)
         gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                     <  1:1519306/150‑1 (MQ=255)
         gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                     <  2:2013561/150‑1 (MQ=255)
         gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                     <  1:783161/150‑1 (MQ=255)
          tCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGc                                                                                                                                    >  2:1456343/1‑150 (MQ=255)
           ccGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCa                                                                                                                                   <  2:4376760/150‑1 (MQ=255)
             gCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATa                                                                                                                                 <  1:402615/150‑1 (MQ=255)
              ccTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa                                                                                                                                >  1:4289286/1‑150 (MQ=255)
                    tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaa                                                                                                                          >  2:871839/1‑150 (MQ=255)
                     gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaac                                                                                                                         >  1:1697531/1‑150 (MQ=255)
                      ccGTTGGCTTTATTTGGAAATAGCCGGGATCAAGCTGCATCCATCTTGAAAAAACTCATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACATATACCAATGTTCCTCATAAAGacaaca                                                                                                                        <  2:3921719/150‑1 (MQ=255)
                       cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACAt                                                                                                                       <  1:4684331/150‑1 (MQ=255)
                         ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACTAGACCAAAGTTCCGCATAAAGACAACAATg                                                                                                                     >  1:529621/1‑150 (MQ=255)
                          tGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTTATCCTTACCGAGACCAAAGTTCCGCAAAAAGACAACAtttc                                                                                                                    >  1:659686/1‑148 (MQ=255)
                               ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACt                                                                                                               <  1:3175957/150‑1 (MQ=255)
                               ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACt                                                                                                               <  1:1676120/150‑1 (MQ=255)
                                 aTGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACttt                                                                                                             <  2:4131315/150‑1 (MQ=255)
                                    tgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTTgc                                                                                                          >  1:2275110/1‑150 (MQ=255)
                                       aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTTTTTATAAACTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATACAACATTGCTTAATTTGGCGCa                                                                                                       >  1:2046002/1‑150 (MQ=255)
                                       aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCTAGACCCATGTTACGCATAAACAAAACAAAGCTTAATTTGGCGCa                                                                                                       >  2:3653946/1‑150 (MQ=255)
                                       aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATAATTACCTAGACCAAATTTACGCATAAAGACAACAATGCTTACTTTTGCGCa                                                                                                       >  1:4428726/1‑150 (MQ=255)
                                        aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                      <  1:1320915/150‑1 (MQ=255)
                                        aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                      >  1:1830739/1‑150 (MQ=255)
                                             ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGAt                                                                                                 <  1:3609409/150‑1 (MQ=255)
                                                ggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAc                                                                                              <  1:1112834/150‑1 (MQ=255)
                                                  aaCAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                            <  2:4532694/150‑1 (MQ=255)
                                                    cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                          <  1:4097898/150‑1 (MQ=255)
                                                    cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                          >  2:4315324/1‑150 (MQ=255)
                                                    cAAGCTGCATACATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                          <  2:2740935/150‑1 (MQ=255)
                                                     aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                         >  2:1394090/1‑150 (MQ=255)
                                                        cTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGcaca                                                                                      <  1:2770802/150‑1 (MQ=255)
                                                        cTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTATACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGcaca                                                                                      >  2:4471315/1‑150 (MQ=255)
                                                         tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACAATGATTAATTTGGCGCAGGCGATGACGAAGCACat                                                                                     >  2:1615886/1‑150 (MQ=255)
                                                           catccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACatat                                                                                   >  2:2611003/1‑150 (MQ=255)
                                                            atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATg                                                                                  <  2:17301/150‑1 (MQ=255)
                                                            atccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATg                                                                                  <  2:16274/150‑1 (MQ=255)
                                                              ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgt                                                                                <  1:3091106/150‑1 (MQ=255)
                                                               catcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtg                                                                               <  2:4174356/150‑1 (MQ=255)
                                                                  cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAc                                                                            <  2:108954/150‑1 (MQ=255)
                                                                   tGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAcc                                                                           <  2:1846312/150‑1 (MQ=255)
                                                                   tGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAcc                                                                           <  2:1968040/150‑1 (MQ=255)
                                                                     gAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAg                                                                         >  2:1459178/1‑150 (MQ=255)
                                                                       aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                       <  1:383421/150‑1 (MQ=255)
                                                                       aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                       >  2:3879536/1‑150 (MQ=255)
                                                                        aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtc                                                                      >  2:4724240/1‑150 (MQ=255)
                                                                        aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtc                                                                      >  2:625388/1‑150 (MQ=255)
                                                                          aaCACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGAACAATGTTACGCATAAAGACAACATTGCTTAATTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtctc                                                                    >  1:2689592/1‑150 (MQ=255)
                                                                           acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                   <  2:3735039/150‑1 (MQ=255)
                                                                           acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                   >  2:4659896/1‑150 (MQ=255)
                                                                              caTCTGATTATCAAACTGTTACGGTCGAGGATTGAAAACGTTGCGCCAGGTACAGTGATCAATACCTAGACCAATGTTCCGCATAAATACAACATTGCTTACTTTTGCGCAGGCGATGACGACGCACATATGGTGTAACAGTTCTCGcag                                                                >  1:1934592/1‑148 (MQ=255)
                                                                                 cTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCAGGTACAGTGATAATTACCTAGACCAAATTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACAAAAGGTGTAACACTTATCGCAgccg                                                             >  1:938628/1‑150 (MQ=255)
                                                                                  tGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTAAAGTGATAATTACCTATAACAAAGTTACGCATAAAGACAACAATGCTTACTTTTGCGCAGGCGATTACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                            >  1:1480549/1‑150 (MQ=255)
                                                                                  tGATTATCAAACTGTTACGGTCGAGTTTTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCTTACTTTTGCGCAGGCGATGACGAAGCACAAATTGTGTACCAGTTATCGCTgccgc                                                            >  1:1661032/1‑150 (MQ=255)
                                                                                   gATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAATACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgcc                                                           >  1:3168861/1‑150 (MQ=255)
                                                                                        tCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTgg                                                      <  1:270056/150‑1 (MQ=255)
                                                                                        tCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTgg                                                      <  1:270824/150‑1 (MQ=255)
                                                                                          aaaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtg                                                    >  2:3305484/1‑150 (MQ=255)
                                                                                            aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgct                                                  <  1:173711/150‑1 (MQ=255)
                                                                                             cTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgctg                                                 <  2:4236946/150‑1 (MQ=255)
                                                                                               gTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCa                                               <  1:4319938/150‑1 (MQ=255)
                                                                                                  aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGc                                            <  1:3741138/150‑1 (MQ=255)
                                                                                                  aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGc                                            <  1:3502023/150‑1 (MQ=255)
                                                                                                    ggTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTCTCGCTGCCGCCACTGGTGCTGCATGCgg                                          >  2:1979386/1‑150 (MQ=255)
                                                                                                     gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                         >  1:3189107/1‑150 (MQ=255)
                                                                                                       cGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGc                                       >  1:283426/1‑150 (MQ=255)
                                                                                                        gATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCa                                      >  1:1600604/1‑150 (MQ=255)
                                                                                                        gATTATTGATAACGATGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCa                                      >  2:3506339/1‑150 (MQ=255)
                                                                                                         attattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaa                                     <  2:2327470/150‑1 (MQ=255)
                                                                                                          ttattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaa                                    >  2:2281349/1‑150 (MQ=255)
                                                                                                            attGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCCTAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaaa                                  >  2:2577954/1‑150 (MQ=255)
                                                                                                             ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                 <  1:115167/150‑1 (MQ=255)
                                                                                                             ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                 <  2:659686/150‑1 (MQ=255)
                                                                                                             ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                 <  2:16961/150‑1 (MQ=255)
                                                                                                             ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                 <  2:3033029/150‑1 (MQ=255)
                                                                                                              tGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                                <  2:2291715/150‑1 (MQ=255)
                                                                                                                aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGa                              >  1:1208914/1‑150 (MQ=255)
                                                                                                                 tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGaa                             >  1:1349813/1‑150 (MQ=255)
                                                                                                                 tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGaa                             >  1:1701052/1‑150 (MQ=255)
                                                                                                                  aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                            >  2:632984/1‑150 (MQ=255)
                                                                                                                  aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                            <  1:871839/150‑1 (MQ=255)
                                                                                                                   aCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                           >  1:3706863/1‑150 (MQ=255)
                                                                                                                   aCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                           >  1:2503666/1‑150 (MQ=255)
                                                                                                                     gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                         >  2:250588/1‑150 (MQ=255)
                                                                                                                       tGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGa                       <  2:182609/150‑1 (MQ=255)
                                                                                                                             aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                 >  2:3054872/1‑150 (MQ=255)
                                                                                                                             aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                 >  1:2208133/1‑150 (MQ=255)
                                                                                                                                tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTGCTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg              <  2:299472/150‑1 (MQ=255)
                                                                                                                                tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg              <  2:2625222/150‑1 (MQ=255)
                                                                                                                                tACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg              >  1:3488194/1‑150 (MQ=255)
                                                                                                                                 aCAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtgt             >  2:1782430/1‑150 (MQ=255)
                                                                                                                                    gTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg          >  2:3380291/1‑150 (MQ=255)
                                                                                                                                         cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc     <  1:1802668/150‑1 (MQ=255)
                                                                                                                                         cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGAGTGGgc     >  1:116896/1‑150 (MQ=255)
                                                                                                                                            tACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCa  >  1:3034410/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA  >  CP009273/1365139‑1365428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA  >  CP009273/1365134‑1365430
                                                                                                                                                     |                                                                                                                                                   
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCT                                                                                                                                                     <  SRR19973082.3758876/150‑1 (MQ=60)
     GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACC                                                                                                                                                <  SRR19973082.305430/150‑1 (MQ=60)
     GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACC                                                                                                                                                <  SRR19973082.305432/150‑1 (MQ=60)
        GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                             <  SRR19973082.4568882/150‑1 (MQ=60)
         AGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATG                                                                                                                                            <  SRR19973082.337601/150‑1 (MQ=60)
          GTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGT                                                                                                                                           >  SRR19973082.2341885/1‑150 (MQ=60)
           TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                          <  SRR19973082.3994042/150‑1 (MQ=60)
             TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATGTTCC                                                                                                                                        >  SRR19973082.1000739/1‑150 (MQ=60)
              GTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACCTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCC                                                                                                                                       >  SRR19973082.3094003/1‑150 (MQ=60)
              GTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCG                                                                                                                                       <  SRR19973082.788174/150‑1 (MQ=60)
              GTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCG                                                                                                                                       <  SRR19973082.1529383/150‑1 (MQ=60)
                CCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                     >  SRR19973082.3905467/1‑150 (MQ=60)
                CCGCCTGGATGCCGTTGGCTGTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                     >  SRR19973082.1500969/1‑150 (MQ=60)
                  GCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATA                                                                                                                                   <  SRR19973082.405009/150‑1 (MQ=60)
                  GCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATA                                                                                                                                   <  SRR19973082.405142/150‑1 (MQ=60)
                  GCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATA                                                                                                                                   <  SRR19973082.1619081/150‑1 (MQ=60)
                   CCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAA                                                                                                                                  <  SRR19973082.3723161/150‑1 (MQ=60)
                   CCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAA                                                                                                                                  >  SRR19973082.4315262/1‑150 (MQ=60)
                      GGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGA                                                                                                                               <  SRR19973082.3825777/150‑1 (MQ=60)
                          GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAATACAAC                                                                                                                           >  SRR19973082.1708815/1‑150 (MQ=60)
                            CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACAT                                                                                                                         <  SRR19973082.4712662/150‑1 (MQ=60)
                            CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACAT                                                                                                                         <  SRR19973082.4712592/150‑1 (MQ=60)
                              TTGGCTTTATGTGGAATGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                       <  SRR19973082.1940728/150‑1 (MQ=60)
                              TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                       <  SRR19973082.1232285/150‑1 (MQ=60)
                              TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                       <  SRR19973082.4338889/150‑1 (MQ=60)
                              TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACTAGACCAAAGTTCCGCATAAAGACAACAATG                                                                                                                       >  SRR19973082.533419/1‑150 (MQ=60)
                               TGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATAATTACCTAGACCAATGTTCCGCATAAAGAAAACATTGC                                                                                                                      >  SRR19973082.3051687/1‑150 (MQ=60)
                               TGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTTATCCTTACCGAGACCAAAGTTCCGCAAAAAGACAACATTTC                                                                                                                      >  SRR19973082.664238/1‑150 (MQ=60)
                               TGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACCTTTCGCCAGGTACAGTTATCATTACCTAGACCAAAGTTCCGCAaaaataaaacaattc                                                                                                                      >  SRR19973082.17206/1‑135 (MQ=60)
                                GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCAATAACAATGTTCCGCATAAATACAAAATTTCT                                                                                                                     >  SRR19973082.3478146/1‑150 (MQ=60)
                                GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATAATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCT                                                                                                                     >  SRR19973082.2712816/1‑150 (MQ=60)
                                  CTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCTTA                                                                                                                   >  SRR19973082.4127158/1‑150 (MQ=60)
                                    TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACT                                                                                                                 <  SRR19973082.3195132/150‑1 (MQ=60)
                                    TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACT                                                                                                                 <  SRR19973082.1687274/150‑1 (MQ=60)
                                    TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACT                                                                                                                 <  SRR19973082.3195444/150‑1 (MQ=60)
                                      ATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTT                                                                                                               <  SRR19973082.1562526/150‑1 (MQ=60)
                                        GTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGG                                                                                                             <  SRR19973082.3029749/150‑1 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                            <  SRR19973082.1469006/150‑1 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTTGC                                                                                                            >  SRR19973082.2289336/1‑150 (MQ=60)
                                            AAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTTTTTATAAACTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATACAACATTGCTTAATTTGGCGCA                                                                                                         >  SRR19973082.2058988/1‑150 (MQ=60)
                                            AAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATAATTACCTAGACCAAATTTACGCATAAAGACAACAATGCTTACTTTTGCGCA                                                                                                         >  SRR19973082.4455557/1‑150 (MQ=60)
                                             AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                        <  SRR19973082.1330041/150‑1 (MQ=60)
                                             AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                        <  SRR19973082.1330291/150‑1 (MQ=60)
                                             AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                        >  SRR19973082.1842693/1‑150 (MQ=60)
                                             AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                        >  SRR19973082.1841592/1‑150 (MQ=60)
                                                  CCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCAGCATAAAGAAAACATTGCTTACTTTGGCGCAGGCGAT                                                                                                   >  SRR19973082.2704875/1‑150 (MQ=60)
                                                  CCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGAT                                                                                                   <  SRR19973082.3631523/150‑1 (MQ=60)
                                                    GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGTTAACGTTTCGCCAGGTACAGTGTTCATTACCAAGACCCATGTTACGCATAAAGACAAAAATGCGTAATTTTGCGCAGGCGATGA                                                                                                 >  SRR19973082.4098932/1‑150 (MQ=60)
                                                     GGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAC                                                                                                <  SRR19973082.2029496/150‑1 (MQ=60)
                                                     GGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAC                                                                                                <  SRR19973082.1120317/150‑1 (MQ=60)
                                                         CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAG                                                                                            <  SRR19973082.4123006/150‑1 (MQ=60)
                                                          AAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGC                                                                                           <  SRR19973082.4252943/150‑1 (MQ=60)
                                                           AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGAACAAAGTTCCGCATAAAGACAACATTTCTTACTTTTGCGCAGGCGATGTCGAAGCA                                                                                          >  SRR19973082.611403/1‑150 (MQ=60)
                                                             CTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACA                                                                                        <  SRR19973082.2787520/150‑1 (MQ=60)
                                                              TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTAACGAGACCAATGTTACGCATAAAGACAACATTTCTTAATTTTGCGCAGGCGATGACGAAGCACAA                                                                                       >  SRR19973082.1743626/1‑150 (MQ=60)
                                                               GCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATA                                                                                      <  SRR19973082.2267965/150‑1 (MQ=60)
                                                                   CCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGT                                                                                  <  SRR19973082.3109976/150‑1 (MQ=60)
                                                                        TGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGTTCATTACCAAGAACAATGTTGCGCATAAAGTCAAAAATGATTTCTTTGGGGCAGGCGAAGTCGAATCACAAATGGTGTACC                                                                             >  SRR19973082.2123964/1‑150 (MQ=60)
                                                                         GGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCAAAAAGACAACCTTGCTTACTTTTGCGCAGGCGATGACGAAGCACAAATGGTGTACCA                                                                            >  SRR19973082.1398145/1‑150 (MQ=60)
                                                                            AAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTT                                                                         <  SRR19973082.385856/150‑1 (MQ=60)
                                                                              AAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCT                                                                       <  SRR19973082.2743064/150‑1 (MQ=60)
                                                                               AACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGAACAATGTTACGCATAAAGACAACATTGCTTAATTTTGCGCAGGCGATGACGAAGCACAAATTGTGTACCAGTTCTC                                                                      >  SRR19973082.2706088/1‑150 (MQ=60)
                                                                                 CACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACCTTGCGCCACGTGCAGTGATCATTACCAAGAACAATGTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGAAGAAGCcaaaaatgtgtaacagttctcgc                                                                    >  SRR19973082.3741925/1‑127 (MQ=60)
                                                                                   CATCTGATTATCAAACTGTTACGGTCGAGGATTGAAAACGTTGCGCCAGGTACAGTGATCAATACCTAGACCAATGTTCCGCATAAATACAACATTGCTTACTTTTGCGCAGGCGATGACGACGCACATATGGTGTAACAGTTCTCGCAG                                                                  >  SRR19973082.1946855/1‑150 (MQ=60)
                                                                                     TCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCC                                                                <  SRR19973082.3951284/150‑1 (MQ=60)
                                                                                      CTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCG                                                               <  SRR19973082.3188617/150‑1 (MQ=60)
                                                                                      CTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCAGGTACAGTGATAATTACCTAGACCAAATTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACAaaaggtgtaacacttatcgcagccg                                                               >  SRR19973082.945143/1‑125 (MQ=60)
                                                                                      CTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAAAGTTCCCCAAAAATAAAACAATTAATACTTTTGCGCAGGCGATGACGAAGCACAAATGGTGTAACAGTTCTCGCagccc                                                               >  SRR19973082.1730203/1‑145 (MQ=60)
                                                                                       TGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTAAAGTGATAATTACCTATAACAAAGTTACGCATAAAGACAACAATGCTTACTTTTGCGCAGGCGATTACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGC                                                              >  SRR19973082.1490487/1‑150 (MQ=60)
                                                                                       TGATTATCAAACTGTTACGGTCGAGTTTTGATAACGTTGCGCCCGGTACAGTGATCATTACCTAGACCAAAGTTACGCATAAAGACAACAATTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGC                                                              >  SRR19973082.1672229/1‑150 (MQ=60)
                                                                                       TGATTATCAAACTGTTACGGTCGAGTTTTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCTTACTTTTGCGCAGGCGATGACGAAGCACAAATTGTGTACCAGTTATCGCTGCCGC                                                              >  SRR19973082.1672090/1‑150 (MQ=60)
                                                                                        GATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTAAAGTGATCATTACCTATACCAATTTTACGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTATCGCTGCCGCC                                                             >  SRR19973082.3187907/1‑150 (MQ=60)
                                                                                        GATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAATACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCC                                                             >  SRR19973082.3187939/1‑150 (MQ=60)
                                                                                         ATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCGAGACCAAAGTTCCGCATAAATACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTACCAGTTCTCGCTGCCcccc                                                            >  SRR19973082.1782369/1‑146 (MQ=60)
                                                                                         ATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATAATTAACAAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTCTCGCTGCCGCCA                                                            >  SRR19973082.1688912/1‑150 (MQ=60)
                                                                                             TCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGG                                                        <  SRR19973082.273057/150‑1 (MQ=60)
                                                                                             TCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGG                                                        <  SRR19973082.272839/150‑1 (MQ=60)
                                                                                             TCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGG                                                        <  SRR19973082.272079/150‑1 (MQ=60)
                                                                                             TCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGG                                                        <  SRR19973082.272067/150‑1 (MQ=60)
                                                                                              CAAACTGTTACGGTCGATTTTTGATAAACTTTCGCCACGTACAGTGATAAATACCTAGAACAATGTTCCGCAAAAATACAACAATTCTTAATTTTGCGCAGGCGGTGACGAAGCACAaaatgtgtaccagttatagctggccccccaggg                                                       >  SRR19973082.126617/1‑117 (MQ=24)
                                                                                                AACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGC                                                     <  SRR19973082.797495/150‑1 (MQ=60)
                                                                                                 ACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCT                                                    <  SRR19973082.175201/150‑1 (MQ=60)
                                                                                                   TGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGATCATTAACAAGAACAAATTTACGCAAAAATAAAACAATGCTTAATTTTGCGCAGGCGATTACGAAGCACAAATTGTGTAACAGTTATCGCTGCCGCCACAGGTGCTGC                                                  >  SRR19973082.1386019/1‑150 (MQ=31)
                                                                                                    GTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCA                                                 <  SRR19973082.4346110/150‑1 (MQ=60)
                                                                                                    GTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCA                                                 <  SRR19973082.4346095/150‑1 (MQ=60)
                                                                                                    GTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCA                                                 <  SRR19973082.4346012/150‑1 (MQ=60)
                                                                                                       ACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGC                                              <  SRR19973082.3764271/150‑1 (MQ=60)
                                                                                                       ACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGC                                              <  SRR19973082.3523426/150‑1 (MQ=60)
                                                                                                         GGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGG                                            >  SRR19973082.353425/1‑150 (MQ=60)
                                                                                                          GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                           >  SRR19973082.3208210/1‑150 (MQ=60)
                                                                                                          GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                           >  SRR19973082.3208449/1‑150 (MQ=60)
                                                                                                            CGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGC                                         >  SRR19973082.285503/1‑150 (MQ=60)
                                                                                                            CGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGC                                         >  SRR19973082.3364106/1‑150 (MQ=60)
                                                                                                             GATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCA                                        >  SRR19973082.1611153/1‑150 (MQ=60)
                                                                                                             GATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCA                                        >  SRR19973082.1610452/1‑150 (MQ=60)
                                                                                                              ATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAA                                       <  SRR19973082.556371/150‑1 (MQ=60)
                                                                                                              ATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAA                                       >  SRR19973082.1908277/1‑150 (MQ=60)
                                                                                                              ATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAA                                       <  SRR19973082.1466185/150‑1 (MQ=60)
                                                                                                                  TTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAC                                   <  SRR19973082.116243/150‑1 (MQ=60)
                                                                                                                     ATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGA                                >  SRR19973082.1217080/1‑150 (MQ=60)
                                                                                                                      TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                               >  SRR19973082.1712365/1‑150 (MQ=60)
                                                                                                                      TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                               >  SRR19973082.1712306/1‑150 (MQ=60)
                                                                                                                      TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                               >  SRR19973082.1359119/1‑150 (MQ=60)
                                                                                                                      TAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAA                               >  SRR19973082.2808479/1‑150 (MQ=60)
                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973082.877808/150‑1 (MQ=60)
                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973082.878360/150‑1 (MQ=60)
                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973082.966784/150‑1 (MQ=60)
                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973082.1137750/150‑1 (MQ=60)
                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973082.1199618/150‑1 (MQ=60)
                                                                                                                        ACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACG                             >  SRR19973082.2519310/1‑150 (MQ=60)
                                                                                                                        ACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACG                             >  SRR19973082.2519294/1‑150 (MQ=60)
                                                                                                                        ACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACG                             >  SRR19973082.3729796/1‑150 (MQ=60)
                                                                                                                               GCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGC                      >  SRR19973082.855330/1‑150 (MQ=60)
                                                                                                                               GCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGC                      <  SRR19973082.3152218/150‑1 (MQ=60)
                                                                                                                               GCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGC                      <  SRR19973082.770657/150‑1 (MQ=60)
                                                                                                                                  AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT                   >  SRR19973082.2222106/1‑150 (MQ=60)
                                                                                                                                   GGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTT                  >  SRR19973082.2162470/1‑150 (MQ=60)
                                                                                                                                   GGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTT                  <  SRR19973082.1002177/150‑1 (MQ=60)
                                                                                                                                     TACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTG                >  SRR19973082.3509563/1‑150 (MQ=60)
                                                                                                                                     TACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTG                >  SRR19973082.3509638/1‑150 (MQ=60)
                                                                                                                                     TACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGAGTACCAGTACTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTG                >  SRR19973082.3509606/1‑150 (MQ=60)
                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGC       <  SRR19973082.1814500/150‑1 (MQ=60)
                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGC       >  SRR19973082.118051/1‑150 (MQ=60)
                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGC       >  SRR19973082.117900/1‑150 (MQ=60)
                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGAGTGGGC       >  SRR19973082.117982/1‑150 (MQ=60)
                                                                                                                                                 TACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCA    >  SRR19973082.3053076/1‑150 (MQ=60)
                                                                                                                                                   CCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA  >  SRR19973082.3474299/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA  >  CP009273/1365134‑1365430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑