Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,365,283	G→T	90.9%	E271* (GAG→TAG)	ycjM →	alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,365,283	0	G	T	90.9%	575.4 / 42.2	227	E271* (GAG→TAG)	ycjM	alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base G (18/4); new base T (95/99); total (124/103)
Fisher's exact test for biased strand distribution p-value = 3.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

GGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAA  >  CP009273/1365136‑1365429

  tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGAc                                                                                                                                                >  2:1890086/1‑150 (MQ=255)

   gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAGGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAcc                                                                                                                                               <  1:1724853/150‑1 (MQ=255)

   gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACCCCTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAcc                                                                                                                                               <  2:4382684/150‑1 (MQ=255)

   gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAcc                                                                                                                                               >  1:1611874/1‑150 (MQ=255)

   gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCAAGAcc                                                                                                                                               >  1:2188414/1‑150 (MQ=255)

    cTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACCGTGATCATTAACTAGAccc                                                                                                                                              >  2:4167252/1‑149 (MQ=255)

      gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                            <  2:3313707/150‑1 (MQ=255)

      gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                            <  2:4233395/150‑1 (MQ=255)

      gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                            <  1:1782847/150‑1 (MQ=255)

      gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAAt                                                                                                                                            <  2:450734/150‑1 (MQ=255)

      gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAAt                                                                                                                                            >  2:4484245/1‑150 (MQ=255)

       aGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg                                                                                                                                           <  2:2419/150‑1 (MQ=255)

       aGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg                                                                                                                                           <  2:2860298/150‑1 (MQ=255)

         tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt                                                                                                                                         >  1:1845949/1‑150 (MQ=255)

          aTGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTc                                                                                                                                        >  1:2502948/1‑150 (MQ=255)

           tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTcc                                                                                                                                       <  1:165313/150‑1 (MQ=255)

               cGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCCAAGTTCCGcca                                                                                                                                   >  1:2372890/1‑148 (MQ=255)

                    ggATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGGTTCGCCACGGACACTGATCAATAACAACACCAAAGTTCCGCAaaaaaa                                                                                                                              >  1:3415797/1‑148 (MQ=255)

                    ggATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGa                                                                                                                              <  2:823385/150‑1 (MQ=255)

                        gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaac                                                                                                                          >  2:2480047/1‑150 (MQ=255)

                          cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGGTCATTAACAATACCAAAGTTACGCATAAATaaaacaa                                                                                                                        >  1:3059554/1‑149 (MQ=255)

                          cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATAATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAt                                                                                                                        >  2:3994173/1‑150 (MQ=255)

                            ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTg                                                                                                                      <  1:1071020/150‑1 (MQ=255)

                                 tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAc                                                                                                                 <  2:517266/150‑1 (MQ=255)

                                 tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAc                                                                                                                 <  1:441823/150‑1 (MQ=255)

                                  ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACt                                                                                                                <  1:2291355/150‑1 (MQ=255)

                                    aTGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTAAttt                                                                                                              >  1:3946897/1‑150 (MQ=255)

                                     tgtgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTg                                                                                                             <  2:4314294/150‑1 (MQ=255)

                                        ggAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgcg                                                                                                          <  1:1219140/150‑1 (MQ=255)

                                            agagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAgg                                                                                                      >  1:495123/1‑150 (MQ=255)

                                             gagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGc                                                                                                     <  2:223802/150‑1 (MQ=255)

                                             gagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGc                                                                                                     <  1:2992336/150‑1 (MQ=255)

                                                ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGAt                                                                                                  <  1:2337669/150‑1 (MQ=255)

                                                 cGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCGTACATTGGCGCAGGCGATg                                                                                                 >  1:2094312/1‑150 (MQ=255)

                                                    gAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACg                                                                                              >  1:1010624/1‑150 (MQ=255)

                                                    gAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACg                                                                                              <  2:1833769/150‑1 (MQ=255)

                                                      aCAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGaa                                                                                            >  1:1877976/1‑150 (MQ=255)

                                                       cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                           <  1:2430786/150‑1 (MQ=255)

                                                       cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                           <  1:1750140/150‑1 (MQ=255)

                                                        aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                          >  2:2164473/1‑150 (MQ=255)

                                                           cTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTGTTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCCGGCGATGACGAAGcaca                                                                                       >  1:3775660/1‑150 (MQ=255)

                                                                tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTAATGATAACCTTGCGCCACGTACACTGATCAATAACAAGACCAATGTTCCGCAAAAAGACAACAATTCTTACTTTTGCGCAGGCGATGACGAAGCACATAAgg                                                                                  >  1:4308148/1‑150 (MQ=255)

                                                                  catcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTTCGCCACGTACACTGATCATTACCAAGAACAAATTTACCCAAAAAGACAACAATTCGTACATTTGCGCAGGCGATGACCAAGcacaaaaagtg                                                                                >  1:2692389/1‑143 (MQ=255)

                                                                     cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAc                                                                             >  1:2246265/1‑150 (MQ=255)

                                                                      tGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTAcc                                                                            >  2:3379987/1‑150 (MQ=255)

                                                                        gAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAg                                                                          >  2:1324787/1‑150 (MQ=255)

                                                                        gAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATCATTAACTAGACCAATGTTACGCATAAAGACAACCTTGCTTACTTTGGCGCAGGCGATGACGCAGCACATATGGTGTACCAg                                                                          >  2:36773/1‑150 (MQ=255)

                                                                         aaaaaaCACATCTGATTATCAAACTGTTACGGTCGATAATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                         >  2:1003302/1‑150 (MQ=255)

                                                                          aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                        >  2:3085323/1‑150 (MQ=255)

                                                                           aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtc                                                                       <  1:401822/150‑1 (MQ=255)

                                                                           aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtc                                                                       <  1:2667354/150‑1 (MQ=255)

                                                                            aaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtct                                                                      <  1:1049183/150‑1 (MQ=255)

                                                                                acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCt                                                                  >  2:655056/1‑150 (MQ=255)

                                                                                 caTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg                                                                 >  2:3702760/1‑150 (MQ=255)

                                                                                 caTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTg                                                                 <  1:1863497/150‑1 (MQ=255)

                                                                                 caTCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTTCGCCAGGTACAGTGATCAATACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACCTAAGGTGTACCAGTTCTCGCTg                                                                 >  1:3253388/1‑150 (MQ=255)

                                                                                  aTCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCAGGTACAGTGATAATTAACGAGACCAATGTTCCGCATAAAGAAAACATTTCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgc                                                                >  1:3244404/1‑150 (MQ=255)

                                                                                   tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTAGCTgcc                                                               >  2:1399483/1‑150 (MQ=255)

                                                                                   tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgcc                                                               <  1:771406/150‑1 (MQ=255)

                                                                                   tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgcc                                                               <  1:243997/150‑1 (MQ=255)

                                                                                         tATCAAACTGTTACGGTCGATTATTTATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACAAATGGTGTACCAGTTCTCGCTTCCGCCAca                                                         >  2:29163/1‑149 (MQ=255)

                                                                                         tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACt                                                         <  2:3577848/150‑1 (MQ=255)

                                                                                         tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACt                                                         <  1:1788205/150‑1 (MQ=255)

                                                                                           tCAAACTGTTACGGTCGATTATTGATAACCTTGCGCCAGGGACAGTGATCAATACCTAGAACAATGTTCCGCATAAACACAACATTGCTTACTTTGGCGCATGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTgg                                                       >  2:4444743/1‑150 (MQ=255)

                                                                                            cAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGt                                                      <  1:2252062/150‑1 (MQ=255)

                                                                                                cTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgctg                                                  <  1:1633592/150‑1 (MQ=255)

                                                                                                    tACGGTCGATTATTGATAACGTTGCGCCAGGTACCGTGATCATTACCTAGACCAATGTTCCGCATAAATACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACAGGTGCTGCATg                                              >  2:3368170/1‑150 (MQ=255)

                                                                                                       ggTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCgg                                           >  1:1423942/1‑150 (MQ=255)

                                                                                                        gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                          <  2:1612884/150‑1 (MQ=255)

                                                                                                        gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                          >  1:2360320/1‑150 (MQ=255)

                                                                                                        gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                          >  1:195255/1‑150 (MQ=255)

                                                                                                        gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                          <  2:1612846/150‑1 (MQ=255)

                                                                                                         tCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTg                                         <  2:3799358/150‑1 (MQ=255)

                                                                                                              tattGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaa                                    >  1:1468605/1‑150 (MQ=255)

                                                                                                                ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                  <  2:2380020/150‑1 (MQ=255)

                                                                                                                ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                  <  2:107879/150‑1 (MQ=255)

                                                                                                                 tGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                                 <  1:773849/150‑1 (MQ=255)

                                                                                                                    tAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGaa                              >  2:1953762/1‑150 (MQ=255)

                                                                                                                     aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                             >  1:1058743/1‑150 (MQ=255)

                                                                                                                      aCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                            <  2:451717/150‑1 (MQ=255)

                                                                                                                        gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                          >  2:559370/1‑150 (MQ=255)

                                                                                                                        gTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                          <  2:213666/150‑1 (MQ=255)

                                                                                                                         ttGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTg                         >  2:2588545/1‑150 (MQ=255)

                                                                                                                           gcgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAg                       <  1:203856/150‑1 (MQ=255)

                                                                                                                           gcgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAg                       <  2:4230148/150‑1 (MQ=255)

                                                                                                                           gcgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAg                       <  1:4246507/150‑1 (MQ=255)

                                                                                                                             gcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgc                     >  2:1490614/1‑150 (MQ=255)

                                                                                                                              ccAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcg                    >  2:1436275/1‑150 (MQ=255)

                                                                                                                                aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                  >  2:3491839/1‑150 (MQ=255)

                                                                                                                                aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                  >  1:1559822/1‑150 (MQ=255)

                                                                                                                                aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                  >  1:4130428/1‑150 (MQ=255)

                                                                                                                                aGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt                  <  1:285434/150‑1 (MQ=255)

                                                                                                                                 ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt                 <  1:3085323/150‑1 (MQ=255)

                                                                                                                                        tGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGt          >  1:2600288/1‑150 (MQ=255)

                                                                                                                                            cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc      >  2:399996/1‑150 (MQ=255)

                                                                                                                                            cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc      >  2:200812/1‑150 (MQ=255)

                                                                                                                                             aTTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg     <  1:2120514/150‑1 (MQ=255)

                                                                                                                                               tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCa   >  2:2817751/1‑150 (MQ=255)

                                                                                                                                               tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCa   >  2:4226166/1‑150 (MQ=255)

                                                                                                                                                aCCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCATGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCaa  <  1:2452230/150‑1 (MQ=255)

GGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAA  >  CP009273/1365136‑1365429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365135‑1365432

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                      <  SRR19973080.1389835/150‑1 (MQ=60)

 GGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAG                                                                                                                                                     <  SRR19973080.160327/150‑1 (MQ=60)

    GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAGGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACC                                                                                                                                                  <  SRR19973080.1734836/150‑1 (MQ=60)

    GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACC                                                                                                                                                  >  SRR19973080.2200252/1‑150 (MQ=60)

    GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACC                                                                                                                                                  >  SRR19973080.1621299/1‑150 (MQ=60)

    GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACACTGATCATTACCGAGACC                                                                                                                                                  >  SRR19973080.4301256/1‑150 (MQ=60)

    GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCAAGACC                                                                                                                                                  >  SRR19973080.2200580/1‑150 (MQ=60)

     CTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCA                                                                                                                                                 <  SRR19973080.1467072/150‑1 (MQ=60)

       GAGTATGTCCGCCTTGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                               <  SRR19973080.1794244/150‑1 (MQ=60)

       GAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAT                                                                                                                                               <  SRR19973080.1793038/150‑1 (MQ=60)

        AGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAG                                                                                                                                              >  SRR19973080.214853/1‑150 (MQ=60)

          TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                            >  SRR19973080.1856321/1‑150 (MQ=60)

          TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                            >  SRR19973080.1856330/1‑150 (MQ=60)

           ATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTC                                                                                                                                           >  SRR19973080.2516761/1‑150 (MQ=60)

           ATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTC                                                                                                                                           >  SRR19973080.1739400/1‑150 (MQ=60)

           ATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTC                                                                                                                                           >  SRR19973080.2516520/1‑150 (MQ=60)

            TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCC                                                                                                                                          <  SRR19973080.166628/150‑1 (MQ=60)

               CCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                       <  SRR19973080.959258/150‑1 (MQ=60)

                CGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCcaagttccgcca                                                                                                                                      >  SRR19973080.2386085/1‑138 (MQ=60)

                CGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACCTTGCGCCAGGGACAGTGATCCTTACCTAGACCAATGTTCCGCAA                                                                                                                                      >  SRR19973080.2386047/1‑150 (MQ=60)

                   CTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCCGGTACACTGATAATTACCTAGACCAATTTTCCGCAAAAA                                                                                                                                   >  SRR19973080.1649155/1‑150 (MQ=60)

                          CCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACA                                                                                                                            <  SRR19973080.1333419/150‑1 (MQ=60)

                           CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCAAAAAGACAACAT                                                                                                                           >  SRR19973080.3264115/1‑150 (MQ=60)

                           CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCTATACCAAATTTACGCATAAAGACAACAG                                                                                                                           >  SRR19973080.3076189/1‑150 (MQ=60)

                            GTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATT                                                                                                                          <  SRR19973080.1779581/150‑1 (MQ=60)

                             TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                         <  SRR19973080.1075068/150‑1 (MQ=60)

                             TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                         <  SRR19973080.1077256/150‑1 (MQ=60)

                             TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                         <  SRR19973080.515911/150‑1 (MQ=60)

                             TTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTG                                                                                                                         <  SRR19973080.2420485/150‑1 (MQ=60)

                                GCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTT                                                                                                                      <  SRR19973080.4249108/150‑1 (MQ=60)

                                GCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTT                                                                                                                      <  SRR19973080.4249459/150‑1 (MQ=60)

                                  TTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAC                                                                                                                    <  SRR19973080.444576/150‑1 (MQ=60)

                                   TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACT                                                                                                                   <  SRR19973080.25913/150‑1 (MQ=60)

                                   TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACT                                                                                                                   <  SRR19973080.2303973/150‑1 (MQ=60)

                                     ATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTTCGCCAGGTAAAGTGATCATTACCAAGACCAATGTTCCGCATAAAGACAACATTTCTTACTTT                                                                                                                 >  SRR19973080.3958006/1‑150 (MQ=60)

                                     ATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTAATTT                                                                                                                 >  SRR19973080.3968640/1‑150 (MQ=60)

                                         GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCG                                                                                                             <  SRR19973080.1226511/150‑1 (MQ=60)

                                         GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCG                                                                                                             <  SRR19973080.1226503/150‑1 (MQ=60)

                                            AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                          >  SRR19973080.1089739/1‑150 (MQ=60)

                                             AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGG                                                                                                         >  SRR19973080.498273/1‑150 (MQ=60)

                                             AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGG                                                                                                         <  SRR19973080.195148/150‑1 (MQ=60)

                                             AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGG                                                                                                         <  SRR19973080.1558461/150‑1 (MQ=60)

                                              GAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGC                                                                                                        <  SRR19973080.3008460/150‑1 (MQ=60)

                                               AGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCG                                                                                                       <  SRR19973080.1822366/150‑1 (MQ=60)

                                                 CCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGAT                                                                                                     <  SRR19973080.2350669/150‑1 (MQ=60)

                                                  CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                                    <  SRR19973080.2043789/150‑1 (MQ=60)

                                                  CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCGTACATTGGCGCAGGCGATG                                                                                                    >  SRR19973080.2106137/1‑150 (MQ=60)

                                                   GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCCAAGTTCCGCATAAAGACAACAATGCTTACTTTTGCGCAGGCGATGT                                                                                                   >  SRR19973080.3208716/1‑150 (MQ=60)

                                                    GGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAC                                                                                                  <  SRR19973080.2519211/150‑1 (MQ=60)

                                                     GAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACGTTGGCGCAGGCGATGACG                                                                                                 >  SRR19973080.1016666/1‑150 (MQ=60)

                                                       ACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAA                                                                                               >  SRR19973080.1888108/1‑150 (MQ=60)

                                                       ACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAA                                                                                               >  SRR19973080.3407964/1‑150 (MQ=60)

                                                       ACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAA                                                                                               <  SRR19973080.3969436/150‑1 (MQ=60)

                                                       ACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAA                                                                                               >  SRR19973080.1888436/1‑150 (MQ=60)

                                                        CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAG                                                                                              <  SRR19973080.2444267/150‑1 (MQ=60)

                                                        CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAG                                                                                              <  SRR19973080.1760241/150‑1 (MQ=60)

                                                         AAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGC                                                                                             <  SRR19973080.1936212/150‑1 (MQ=60)

                                                         AAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGC                                                                                             <  SRR19973080.2348780/150‑1 (MQ=60)

                                                          AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTCCGCAAAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCA                                                                                            >  SRR19973080.2793185/1‑150 (MQ=60)

                                                            CTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTGTTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCCGGCGATGACGAAGCACA                                                                                          >  SRR19973080.3796188/1‑150 (MQ=60)

                                                            CTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTGTTGATAACGTTGCGCCAGGTACAGGGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACA                                                                                          >  SRR19973080.221541/1‑150 (MQ=60)

                                                               CATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATAT                                                                                       <  SRR19973080.2453308/150‑1 (MQ=60)

                                                                 TCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTAATGATAACCTTGCGCCACGTACACTGATCAATAACAAGACCAATGTTCCGCAAAAAGACAACAATTCTTACTTTTGCGCAGGCGATGACGAAGCACATAAGG                                                                                     >  SRR19973080.4331693/1‑150 (MQ=60)

                                                                     TCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTA                                                                                 <  SRR19973080.1029653/150‑1 (MQ=60)

                                                                      CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGATCCTTACCTAGACCAAAGTTCCGCCTAAAGACAACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAC                                                                                >  SRR19973080.2259519/1‑150 (MQ=60)

                                                                      CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAA                                                                                >  SRR19973080.2258578/1‑150 (MQ=60)

                                                                         GAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACATTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAG                                                                             >  SRR19973080.1686486/1‑150 (MQ=60)

                                                                            AAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTC                                                                          <  SRR19973080.404221/150‑1 (MQ=60)

                                                                            AAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTC                                                                          <  SRR19973080.2681851/150‑1 (MQ=60)

                                                                             AAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCT                                                                         <  SRR19973080.1055354/150‑1 (MQ=60)

                                                                             AAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCT                                                                         <  SRR19973080.1055415/150‑1 (MQ=60)

                                                                             AAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCT                                                                         <  SRR19973080.1631625/150‑1 (MQ=60)

                                                                                CACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTTCGCCACGTACAGTGATCAATACCAAGACCAAAGTTACGCAAAAAAAAAAAAATGCTTAAATTTGCGCAGGCGCTGACGAAGCACAAATGGTGTAACAGTTatagc                                                                      >  SRR19973080.2690610/1‑145 (MQ=39)

                                                                                  CATCTGATTATCAAACTGTTACGGTCGATTTTTTATAACCTTGAGCCACGTACACTGATAAATAACGAGACCAATGTTACGCATAAATAAAACAATTATTACATTTGCGCAGGCGATGTCGAAGCACAAATTGTGTAACAGTTCTCGCAG                                                                    >  SRR19973080.3059528/1‑150 (MQ=28)

                                                                                  CATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTG                                                                    <  SRR19973080.1873841/150‑1 (MQ=60)

                                                                                  CATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTG                                                                    <  SRR19973080.1873917/150‑1 (MQ=60)

                                                                                  CATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTTCGCCAGGTACAGTGATCAATACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACCTAAGGTGTACCAGTTCTCGCTG                                                                    >  SRR19973080.3271295/1‑150 (MQ=60)

                                                                                   ATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCGAGACCAAAGTTCCGCATAAAGACAACATTTCTTAATTTGGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTATCGCTGC                                                                   >  SRR19973080.3262071/1‑150 (MQ=60)

                                                                                   ATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTTCGCCAGGTACAGTGATAATTAACGAGACCAATGTTCCGCATAAAGAAAACATTTCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGC                                                                   >  SRR19973080.3262267/1‑150 (MQ=60)

                                                                                    TCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCC                                                                  <  SRR19973080.775806/150‑1 (MQ=60)

                                                                                    TCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCC                                                                  <  SRR19973080.775792/150‑1 (MQ=60)

                                                                                    TCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCC                                                                  <  SRR19973080.245682/150‑1 (MQ=60)

                                                                                    TCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCC                                                                  <  SRR19973080.246466/150‑1 (MQ=60)

                                                                                         TTATCAAACTGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGATAATTAACAAGACCAAATTTACGCAAAAAGACAACAATGCTTAAATTTGCGCAGGCGATGACCAAGCACAAAAGGTGTAAAAGTTATAGCTGCCGCCAC                                                             >  SRR19973080.397057/1‑150 (MQ=32)

                                                                                          TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                            <  SRR19973080.1798412/150‑1 (MQ=60)

                                                                                           ATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATTACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACAG                                                           >  SRR19973080.2656299/1‑150 (MQ=60)

                                                                                             CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATACAACATTGCTTAATTTGGCGCAGGCGATTACGAAGCACATATGGTGTAACAGTTCTCGCTGCCGCCACTGGT                                                         >  SRR19973080.3764447/1‑150 (MQ=60)

                                                                                             CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGT                                                         <  SRR19973080.2264384/150‑1 (MQ=60)

                                                                                             CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGT                                                         <  SRR19973080.1687657/150‑1 (MQ=60)

                                                                                                 CTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTG                                                     <  SRR19973080.1643160/150‑1 (MQ=60)

                                                                                                     TACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATG                                                 <  SRR19973080.3564423/150‑1 (MQ=60)

                                                                                                       CGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCG                                               >  SRR19973080.4068630/1‑150 (MQ=60)

                                                                                                        GGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGG                                              >  SRR19973080.1432306/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.2275796/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.2373393/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.2420613/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.2275837/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.2742260/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.2373452/1‑150 (MQ=60)

                                                                                                         GTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGT                                             >  SRR19973080.196727/1‑150 (MQ=60)

                                                                                                           CGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGC                                           >  SRR19973080.447938/1‑150 (MQ=60)

                                                                                                           CGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGC                                           >  SRR19973080.445026/1‑150 (MQ=60)

                                                                                                               TATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAA                                       >  SRR19973080.1477061/1‑150 (MQ=60)

                                                                                                                  TGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACA                                    <  SRR19973080.778257/150‑1 (MQ=60)

                                                                                                                  TGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACA                                    <  SRR19973080.4381662/150‑1 (MQ=60)

                                                                                                                   GATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAG                                   >  SRR19973080.1580115/1‑150 (MQ=60)

                                                                                                                    ATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGA                                  >  SRR19973080.2704893/1‑150 (MQ=60)

                                                                                                                      AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                                >  SRR19973080.1064946/1‑150 (MQ=60)

                                                                                                                            GCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAG                          <  SRR19973080.205388/150‑1 (MQ=60)

                                                                                                                            GCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAG                          <  SRR19973080.205370/150‑1 (MQ=60)

                                                                                                                            GCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAG                          <  SRR19973080.4269677/150‑1 (MQ=60)

                                                                                                                                 AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT                     >  SRR19973080.1568885/1‑150 (MQ=60)

                                                                                                                                 AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT                     <  SRR19973080.287283/150‑1 (MQ=60)

                                                                                                                                 AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT                     >  SRR19973080.4152802/1‑150 (MQ=60)

                                                                                                                                  GGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTT                    <  SRR19973080.3102204/150‑1 (MQ=60)

                                                                                                                                         TGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGT             >  SRR19973080.2614427/1‑150 (MQ=60)

                                                                                                                                            TCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGG          >  SRR19973080.2915065/1‑150 (MQ=60)

                                                                                                                                              ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG        <  SRR19973080.2132478/150‑1 (MQ=60)

                                                                                                                                              ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG        <  SRR19973080.2132406/150‑1 (MQ=60)

                                                                                                                                                 ACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCATGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAA     <  SRR19973080.2465816/150‑1 (MQ=60)

                                                                                                                                                  CCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA    >  SRR19973080.2674791/1‑150 (MQ=60)

                                                                                                                                                    TAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  SRR19973080.740135/1‑150 (MQ=60)

                                                                                                                                                    TAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  SRR19973080.2822674/1‑150 (MQ=60)

AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAAC  >  CP009273/1365135‑1365432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`