Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,365,283 G→T 100% E271* (GAG→TAG)  ycjM → alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,365,2830GT99.2% 479.5 / ‑5.6 179E271* (GAG→TAG) ycjMalpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base T (98/74);  minor base A (5/0);  total (104/75)
Fisher's exact test for biased strand distribution p-value = 7.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

GTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365137‑1365426
                                                                                                                                                  |                                                                                                                                               
gTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCga                                                                                                                                                >  1:1590000/1‑148 (MQ=255)
 tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtag                                                                                                                                               <  1:1796695/148‑1 (MQ=255)
 tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtag                                                                                                                                               <  1:1796694/148‑1 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGTAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACTCATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga                                                                                                                                              <  2:2793321/148‑1 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCtaga                                                                                                                                              <  2:3120732/148‑1 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCCGGTACAGTGATAATTACCtaga                                                                                                                                              >  2:1870440/1‑148 (MQ=255)
  gCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACtaga                                                                                                                                              >  1:3330312/1‑148 (MQ=255)
     gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCa                                                                                                                                           >  2:2520145/1‑148 (MQ=255)
       gTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                         <  1:1744851/148‑1 (MQ=255)
        tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATTAAGTTTGGCCAGGTAAAGTGATAAATAGCTAGAGCAAtt                                                                                                                                        >  1:1518521/1‑147 (MQ=255)
        tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATg                                                                                                                                        >  2:664740/1‑148 (MQ=255)
         aTGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGt                                                                                                                                       >  1:2215466/1‑148 (MQ=255)
          tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGtt                                                                                                                                      <  1:2969371/148‑1 (MQ=255)
           gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTc                                                                                                                                     >  1:1404353/1‑148 (MQ=255)
              cGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCTAGACCCATGTTACGc                                                                                                                                  >  1:618163/1‑148 (MQ=255)
                    gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                            <  2:67304/148‑1 (MQ=255)
                    gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                            <  1:815541/148‑1 (MQ=255)
                     aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGa                                                                                                                           <  2:2068357/148‑1 (MQ=255)
                      tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac                                                                                                                          <  2:2641012/148‑1 (MQ=255)
                       gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                         >  2:3013414/1‑148 (MQ=255)
                       gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                         <  1:51066/148‑1 (MQ=255)
                         cGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGGTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaac                                                                                                                       <  1:3060116/148‑1 (MQ=255)
                          gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaaca                                                                                                                      >  2:2180882/1‑148 (MQ=255)
                           ttGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAt                                                                                                                     <  2:1361790/148‑1 (MQ=255)
                               cTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCt                                                                                                                 <  2:1552455/148‑1 (MQ=255)
                               cTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCt                                                                                                                 <  2:1552861/148‑1 (MQ=255)
                                tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCtt                                                                                                                >  1:1182977/1‑148 (MQ=255)
                                tttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCACGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCtt                                                                                                                >  1:659598/1‑148 (MQ=255)
                                 ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTa                                                                                                               <  1:1072393/148‑1 (MQ=255)
                                   aTGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAATACAACAATGCTTACt                                                                                                             >  1:2006190/1‑148 (MQ=255)
                                     gtgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACttt                                                                                                           <  1:2988117/148‑1 (MQ=255)
                                      tgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTg                                                                                                          <  2:3479754/148‑1 (MQ=255)
                                       ggAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTgg                                                                                                         >  2:2435042/1‑148 (MQ=255)
                                        gAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgc                                                                                                        >  1:848515/1‑148 (MQ=255)
                                         aaaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTACGCCTAAAGACAACAATGCTTACTTTGgcg                                                                                                       >  1:75418/1‑148 (MQ=255)
                                          aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACCTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgcgc                                                                                                      >  2:1380651/1‑148 (MQ=255)
                                           agagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAAATTGCTTACTTTTGCGCa                                                                                                     >  1:1369575/1‑148 (MQ=255)
                                            gagCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                    >  2:3282542/1‑148 (MQ=255)
                                             agCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAAAACAATTCTTAATTTTGCGCAgg                                                                                                   >  1:1018038/1‑148 (MQ=255)
                                               ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCg                                                                                                 <  2:2970381/148‑1 (MQ=255)
                                                      cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                          <  2:3330305/148‑1 (MQ=255)
                                                      cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                          <  2:844860/148‑1 (MQ=255)
                                                      cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGa                                                                                          <  2:968614/148‑1 (MQ=255)
                                                       aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGaa                                                                                         <  2:2635260/148‑1 (MQ=255)
                                                        aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                        >  1:2646699/1‑148 (MQ=255)
                                                         gCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                       <  2:2279067/148‑1 (MQ=255)
                                                           tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGcac                                                                                     <  1:998250/148‑1 (MQ=255)
                                                            gCATACATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACTGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAATTTGTCGCAGGCGATGACTAAGcaca                                                                                    <  2:615316/148‑1 (MQ=255)
                                                               tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACatat                                                                                 >  2:2749971/1‑148 (MQ=255)
                                                               tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATTTTCCGCATAAAGAAAACATTGCTTAATTTGGCGCATGCGATTACGAAGCACatat                                                                                 >  1:2774519/1‑148 (MQ=255)
                                                                ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCACGTACAGTGATCATTACCTAGACCAATGTTACGCAAAAAGACAACATTGCTTACTTTGGCGCCGGCGATGACGAAGCACATATg                                                                                >  1:1661085/1‑148 (MQ=255)
                                                                 catcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATgg                                                                               <  1:422243/148‑1 (MQ=255)
                                                                   tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGAAAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGgtg                                                                             >  2:351511/1‑148 (MQ=255)
                                                                   tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTAAAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtg                                                                             >  2:437402/1‑148 (MQ=255)
                                                                      ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAc                                                                          >  1:2084979/1‑148 (MQ=255)
                                                                      ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACCTATGGTGTAc                                                                          >  2:3101591/1‑148 (MQ=255)
                                                                         aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAg                                                                       >  2:2316344/1‑148 (MQ=255)
                                                                          aaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                      <  2:1206288/148‑1 (MQ=255)
                                                                               acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                 >  2:2913600/1‑148 (MQ=255)
                                                                               acaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                 <  2:610775/148‑1 (MQ=255)
                                                                                   cTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgc                                                             <  1:2407370/148‑1 (MQ=255)
                                                                                   cTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGGGATCATTAACAAGAACAATTTTACGCATAAAGACAACATTGCTTACTTTGGCGCCGGCGCTGACGAAGCACATATGGTGTACCCGTTCTCGCTgc                                                             >  2:2565700/1‑148 (MQ=255)
                                                                                     gATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccg                                                           >  2:2336806/1‑148 (MQ=255)
                                                                                      aTTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgc                                                          <  2:796713/148‑1 (MQ=255)
                                                                                       ttATCAAACTGTTACGGTCGAGTATTTATAAAGTTTCGCCAGGTACAGTGATAATTACCAAGAACAAAGTTACGCATAAAGACAAAAATTCTTACTTTTGCGCAGGCGATGACGAAGCACAAAAGGTGTACCAGTTCTAGCAgccccc                                                         >  1:2712004/1‑148 (MQ=255)
                                                                                        tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCa                                                        <  1:2305117/148‑1 (MQ=255)
                                                                                          tCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACt                                                      >  1:823950/1‑148 (MQ=255)
                                                                                             aaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGt                                                   >  2:1260071/1‑148 (MQ=255)
                                                                                              aCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCAAGACCAATTTTACGCATAAATACAACAATTCTTAATTTGGCGCAGGCGATGACGAAGCACATATTGTGTAACAGTTCTCGCTGCCGCCACAGGtg                                                  >  1:451070/1‑148 (MQ=255)
                                                                                                tGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgct                                                >  2:147582/1‑148 (MQ=255)
                                                                                                  ttACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATTTTACGCATAAAGACAACAATTCTTACTTTTGCGCAGGCGATGACGAAGCACAAATGGTGTACCAGTTCTAGCTGCCGCCACAGGtgctgc                                              >  2:911309/1‑148 (MQ=255)
                                                                                                    aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCTGGCGATGACGAAGCACATTTGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCAt                                            <  2:2699490/148‑1 (MQ=255)
                                                                                                    aCGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCAt                                            <  2:887961/148‑1 (MQ=255)
                                                                                                     cGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGGTCTCGCTGCCGCCACTGGTGCTGCATg                                           >  2:600467/1‑148 (MQ=255)
                                                                                                                 gATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                               <  1:3249824/148‑1 (MQ=255)
                                                                                                                  aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              >  2:2384516/1‑148 (MQ=255)
                                                                                                                  aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              >  2:1627260/1‑148 (MQ=255)
                                                                                                                  aTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACa                              <  2:133996/148‑1 (MQ=255)
                                                                                                                    aaCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGa                            <  1:120093/148‑1 (MQ=255)
                                                                                                                      cGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                          <  1:1307943/148‑1 (MQ=255)
                                                                                                                        ttGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                        >  1:1117749/1‑148 (MQ=255)
                                                                                                                        ttGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                        >  1:2895086/1‑148 (MQ=255)
                                                                                                                         tGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                       >  1:2521354/1‑148 (MQ=255)
                                                                                                                          gcgcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTg                      >  2:3501591/1‑148 (MQ=255)
                                                                                                                            gcCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAg                    <  2:848515/148‑1 (MQ=255)
                                                                                                                              cAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgc                  >  1:856008/1‑148 (MQ=255)
                                                                                                                                ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgcgc                >  1:1465528/1‑148 (MQ=255)
                                                                                                                                 gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               >  2:394028/1‑148 (MQ=255)
                                                                                                                                 gTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCt               <  1:1260071/148‑1 (MQ=255)
                                                                                                                                    cAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtg            <  1:665104/148‑1 (MQ=255)
                                                                                                                                       tGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGc         >  1:2140446/1‑148 (MQ=255)
                                                                                                                                       tGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGc         >  2:3142578/1‑148 (MQ=255)
                                                                                                                                        gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  2:743098/1‑148 (MQ=255)
                                                                                                                                        gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        <  2:1590000/148‑1 (MQ=255)
                                                                                                                                        gATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCg        >  2:1150127/1‑148 (MQ=255)
                                                                                                                                           cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTgg     >  1:2832504/1‑148 (MQ=255)
                                                                                                                                             ttACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc   >  1:2804652/1‑148 (MQ=255)
                                                                                                                                              tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  2:2160180/1‑148 (MQ=255)
                                                                                                                                              tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  1:903956/1‑148 (MQ=255)
                                                                                                                                              tACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg  >  1:1974769/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365137‑1365426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365134‑1365426
                                                                                                                                                     |                                                                                                                                               
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCT                                                                                                                                                 >  SRR19973066.1049360/1‑150 (MQ=60)
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCT                                                                                                                                                 <  SRR19973066.1154033/150‑1 (MQ=60)
 AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCGA                                                                                                                                                >  SRR19973066.1601505/1‑150 (MQ=60)
 AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCAA                                                                                                                                                >  SRR19973066.1601498/1‑150 (MQ=60)
   GTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACTAGA                                                                                                                                              >  SRR19973066.3352451/1‑150 (MQ=60)
    TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAC                                                                                                                                             <  SRR19973066.1809470/150‑1 (MQ=60)
    TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAC                                                                                                                                             <  SRR19973066.1809471/150‑1 (MQ=60)
          GTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGT                                                                                                                                       <  SRR19973066.1757412/150‑1 (MQ=60)
          GTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGT                                                                                                                                       >  SRR19973066.2230803/1‑150 (MQ=60)
            ATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTC                                                                                                                                     >  SRR19973066.1414459/1‑150 (MQ=60)
             TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCC                                                                                                                                    <  SRR19973066.2683544/150‑1 (MQ=60)
             TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCC                                                                                                                                    <  SRR19973066.2989004/150‑1 (MQ=60)
             TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCC                                                                                                                                    >  SRR19973066.1363737/1‑150 (MQ=60)
               TCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCTAGACCCATGTTACGC                                                                                                                                  >  SRR19973066.622523/1‑150 (MQ=60)
                       GATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAC                                                                                                                          <  SRR19973066.821518/150‑1 (MQ=60)
                          GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAC                                                                                                                       <  SRR19973066.2599246/150‑1 (MQ=60)
                          GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAC                                                                                                                       <  SRR19973066.51722/150‑1 (MQ=60)
                            CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGGTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAT                                                                                                                     <  SRR19973066.3080582/150‑1 (MQ=60)
                                GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCT                                                                                                                 <  SRR19973066.251003/150‑1 (MQ=60)
                                 GCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTT                                                                                                                >  SRR19973066.1191285/1‑150 (MQ=60)
                                 GCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTT                                                                                                                >  SRR19973066.1190631/1‑150 (MQ=60)
                                 GCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCACGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTT                                                                                                                >  SRR19973066.664416/1‑150 (MQ=60)
                                    TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAATACAACAATGCTTACT                                                                                                             >  SRR19973066.2020267/1‑150 (MQ=60)
                                    TTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACT                                                                                                             <  SRR19973066.1080251/150‑1 (MQ=60)
                                        GTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGG                                                                                                         <  SRR19973066.3007882/150‑1 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATACCAATGTTCCGCATAAAGACAACAATGCTTACTTTGGC                                                                                                        >  SRR19973066.92913/1‑150 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                        <  SRR19973066.2295171/150‑1 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                        <  SRR19973066.508643/150‑1 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                        >  SRR19973066.854735/1‑150 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                        >  SRR19973066.854661/1‑150 (MQ=60)
                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                        >  SRR19973066.854585/1‑150 (MQ=60)
                                          GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTACGCCTAAAGACAACAATGCTTACTTTGGCG                                                                                                       >  SRR19973066.76303/1‑150 (MQ=60)
                                          GGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATACAACAATGCTTACATTTGCG                                                                                                       >  SRR19973066.76284/1‑150 (MQ=60)
                                            AAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGTTAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAAATTGCTTACTTTTGCGCA                                                                                                     >  SRR19973066.1379483/1‑150 (MQ=60)
                                              AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGAAAACAATTCTTAATTTTGCGCAGG                                                                                                   >  SRR19973066.1025531/1‑150 (MQ=60)
                                              AGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGG                                                                                                   <  SRR19973066.817177/150‑1 (MQ=60)
                                                   CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                              <  SRR19973066.1459396/150‑1 (MQ=60)
                                                    GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGGTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGT                                                                                             >  SRR19973066.1754581/1‑150 (MQ=60)
                                                       AACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGGTGCGCCAGGTACAGTGGTCATTTCCGAGACCCATGTTGCGCATAAAGACAACAATGGTTACGTTGGCGCAGGCGATGACGT                                                                                          >  SRR19973066.3004050/1‑150 (MQ=60)
                                                         CAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAG                                                                                        >  SRR19973066.2664480/1‑150 (MQ=60)
                                                           AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGACAACAATGCTTACATTGGCGCAGGCGATGACGAAGCA                                                                                      >  SRR19973066.1617783/1‑150 (MQ=60)
                                                             CTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACA                                                                                    >  SRR19973066.866249/1‑150 (MQ=60)
                                                              TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                   <  SRR19973066.380835/150‑1 (MQ=60)
                                                              TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                   <  SRR19973066.1005603/150‑1 (MQ=60)
                                                                CATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATTTTCCGCATAAAGAAAACATTGCTTAATTTGGCGCATGCGATTACGAAGCACATAT                                                                                 >  SRR19973066.2793198/1‑150 (MQ=60)
                                                                CATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTTATAAAGTTTCGCCAGGTACAGTGATCATTAACTAGACCAATGTTACGCATAAAGACAACATTTCTTAATTTGGCGCAGGCGTTGACGAATCACCTAT                                                                                 >  SRR19973066.2793204/1‑150 (MQ=60)
                                                                CATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATAATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGCTGACGAAGCACATAT                                                                                 >  SRR19973066.2926620/1‑150 (MQ=60)
                                                                 ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCACGTACAGTGATCATTACCTAGACCAATGTTACGCAAAAAGACAACATTGCTTACTTTGGCGCCGGCGATGACGAAGCACATATG                                                                                >  SRR19973066.1673189/1‑150 (MQ=60)
                                                                    CATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTG                                                                             <  SRR19973066.425524/150‑1 (MQ=60)
                                                                       CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGTTCATTACCTATACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAC                                                                          >  SRR19973066.2098653/1‑150 (MQ=60)
                                                                       CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAC                                                                          >  SRR19973066.2099359/1‑150 (MQ=60)
                                                                       CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCAAAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAC                                                                          >  SRR19973066.2099383/1‑150 (MQ=60)
                                                                           AAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACCGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGT                                                                      >  SRR19973066.1926673/1‑150 (MQ=60)
                                                                                     TCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGTTCATTACCTACACCAATGTTCCCCATAAAGACAACATTGCTTACTTTGGCGCAGTCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCC                                                            >  SRR19973066.220261/1‑150 (MQ=60)
                                                                                      CTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCG                                                           <  SRR19973066.2423659/150‑1 (MQ=60)
                                                                                      CTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCG                                                           <  SRR19973066.1539750/150‑1 (MQ=60)
                                                                                       TGATTATCAAACTGTTACGGTCGAGTATTGTTAAAGTTGCGCCAGGTACAGTTATAATTTCCTATACCAAATTTACGCATAAATACAAAAATGCTTACTTTTGGGCAGGCGATGACGAAGCACAaaatgtgttgcacttctagctgccgc                                                          >  SRR19973066.2120450/1‑124 (MQ=34)
                                                                                        GATTATCAAACTGTTACGGTCGAGTATTTATAAAGTTTCGCCAGGTACAGTGATAATTACCAAGAACAAAGTTACGCATAAAGACAAAAATTCTTACTTTTGCGCAGGCGATGACGAAGCACAAAAGGTGTACCAGTTCTagcagccccc                                                         >  SRR19973066.2730214/1‑140 (MQ=60)
                                                                                         ATTATCAAACTGTTACGGTCGAGTATTTATAACGTTGCGCCAGGGACAGTGATCATTACCTAGACCAAAGTTACGCATAAAGACAACAATGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTATCGCTGCCGCCA                                                        >  SRR19973066.1094252/1‑150 (MQ=60)
                                                                                          TTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCAC                                                       <  SRR19973066.3298152/150‑1 (MQ=60)
                                                                                           TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                      <  SRR19973066.2320903/150‑1 (MQ=60)
                                                                                           TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                      >  SRR19973066.829964/1‑150 (MQ=60)
                                                                                             TCAAACTGTTACGGTCGATTATTGATAAAGTTTCGCCAGGTAACGTGATAAATAACAAGACCAATTTTCCGCAAAAAGACAACATTGCTTAATTTTGCGCAGGCGATTACGAATCACATATGGTGTACAAGTACACGCTGCCGCCACatg                                                    >  SRR19973066.1791999/1‑147 (MQ=57)
                                                                                              CAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATACAACAATGCTTACTTTTGCGCAGGCGATGACGAAGCACAAATTGTGTAACAGTTATCGCTGCCGCCACAGGT                                                   >  SRR19973066.545525/1‑150 (MQ=60)
                                                                                               AAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCAAGACCAATTTTACGCATAAATACAACAATTCTTAATTTGGCGCAGGCGATGACGAAGCACATATTGTGTAACAGTTCTCGCTGCCGCCACAGGTG                                                  >  SRR19973066.454537/1‑150 (MQ=60)
                                                                                                 ACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACAAGAACAATGTTCCGCATAAAGAAAACAATGCTTAATTTGGCGCAGGCGATGACGAAGCACAAATTGTGTACCAGTTCTAGCTGCCGCCACAGGTGCT                                                >  SRR19973066.1193180/1‑150 (MQ=60)
                                                                                                           TCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTG                                      <  SRR19973066.1210554/150‑1 (MQ=60)
                                                                                                                    GATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAG                             <  SRR19973066.3271236/150‑1 (MQ=60)
                                                                                                                    GATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAG                             <  SRR19973066.1487885/150‑1 (MQ=60)
                                                                                                                     ATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGA                            >  SRR19973066.2776606/1‑150 (MQ=60)
                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                          <  SRR19973066.121309/150‑1 (MQ=60)
                                                                                                                         CGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGT                        >  SRR19973066.1125809/1‑150 (MQ=60)
                                                                                                                         CGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGT                        <  SRR19973066.1317431/150‑1 (MQ=60)
                                                                                                                         CGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGT                        >  SRR19973066.2914438/1‑150 (MQ=60)
                                                                                                                         CGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGT                        <  SRR19973066.1317415/150‑1 (MQ=60)
                                                                                                                          GTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTT                       >  SRR19973066.2538501/1‑150 (MQ=60)
                                                                                                                               GCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGC                  >  SRR19973066.862185/1‑150 (MQ=60)
                                                                                                                                 CAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGC                >  SRR19973066.1475873/1‑150 (MQ=60)
                                                                                                                                  AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT               <  SRR19973066.3033493/150‑1 (MQ=60)
                                                                                                                                    GTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTT             <  SRR19973066.1269161/150‑1 (MQ=60)
                                                                                                                                       CAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTG          <  SRR19973066.669953/150‑1 (MQ=60)
                                                                                                                                       CAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTG          <  SRR19973066.669960/150‑1 (MQ=60)
                                                                                                                                        AGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGC         >  SRR19973066.2155299/1‑150 (MQ=60)
                                                                                                                                           GATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTG      <  SRR19973066.3106996/150‑1 (MQ=60)
                                                                                                                                            ATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGG     >  SRR19973066.2851542/1‑150 (MQ=60)
                                                                                                                                            ATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGG     >  SRR19973066.2851468/1‑150 (MQ=60)
                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGC   >  SRR19973066.2823525/1‑150 (MQ=60)
                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  SRR19973066.1989259/1‑150 (MQ=60)
                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  SRR19973066.910334/1‑150 (MQ=60)
                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  SRR19973066.1988725/1‑150 (MQ=60)
                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  SRR19973066.626439/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                               
AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG  >  CP009273/1365134‑1365426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑