Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F78 I0 R1	626	241.3	10389380	77.7%	8072548	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,365,283	G→T	81.0%	E271* (GAG→TAG)	ycjM →	alpha amylase catalytic domain family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,365,283	0	G	T	81.0%	467.4 / 137.4	247	E271* (GAG→TAG)	ycjM	alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand): ref base G (13/34); new base T (100/94); total (119/128)
Fisher's exact test for biased strand distribution p-value = 3.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

Only 100 of 247 total aligned reads displayed.

TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAA  >  CP009273/1365138‑1365431

tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAc                                                                                                                                                  >  1:3394674/1‑150 (MQ=255)

tGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGACTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAc                                                                                                                                                  >  1:696403/1‑150 (MQ=255)

    gAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAt                                                                                                                                              >  2:1473215/1‑150 (MQ=255)

       tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGTAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGtt                                                                                                                                           <  1:3721230/150‑1 (MQ=255)

       tATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGtt                                                                                                                                           <  2:4115985/150‑1 (MQ=255)

        aTGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGGACAGTGATCATTACCTAGACCAATGTTc                                                                                                                                          >  2:121129/1‑150 (MQ=255)

         tGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTcc                                                                                                                                         >  1:656063/1‑150 (MQ=255)

          gTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCg                                                                                                                                        >  2:4443380/1‑150 (MQ=255)

            ccGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCa                                                                                                                                      <  1:3730973/150‑1 (MQ=255)

             cGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCAt                                                                                                                                     <  2:2581600/150‑1 (MQ=255)

               ccTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATaa                                                                                                                                   <  2:510609/150‑1 (MQ=255)

                 tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                                 <  1:2438706/150‑1 (MQ=255)

                 tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAg                                                                                                                                 <  2:1198030/150‑1 (MQ=255)

                 tGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAg                                                                                                                                 >  1:1560120/1‑150 (MQ=255)

                  ggATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGNTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATAACAATGTTACGCATAAAGa                                                                                                                                >  1:4786401/1‑150 (MQ=255)

                   gATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGac                                                                                                                               <  2:1901869/150‑1 (MQ=255)

                    aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                              <  2:2929214/150‑1 (MQ=255)

                    aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                              <  2:291864/150‑1 (MQ=255)

                    aTGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGaca                                                                                                                              <  2:2979034/150‑1 (MQ=255)

                     tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaa                                                                                                                             <  1:4505124/150‑1 (MQ=255)

                     tGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaa                                                                                                                             <  1:1916857/150‑1 (MQ=255)

                      gCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGacaac                                                                                                                            >  2:4200222/1‑150 (MQ=255)

                       ccGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGacaaca                                                                                                                           <  1:778999/150‑1 (MQ=255)

                         gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAtt                                                                                                                         >  1:5013849/1‑150 (MQ=255)

                         gTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAtt                                                                                                                         >  2:234592/1‑150 (MQ=255)

                            ggCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATAACAAGACCAATGTTACGCATAAAGACAACATTGCt                                                                                                                      >  1:1364662/1‑150 (MQ=255)

                                ttATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACt                                                                                                                  >  2:1073145/1‑150 (MQ=255)

                                 tATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACtt                                                                                                                 <  1:1073145/150‑1 (MQ=255)

                                   tgtgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTg                                                                                                               <  2:2295567/150‑1 (MQ=255)

                                     tgGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGgc                                                                                                             <  1:1031646/150‑1 (MQ=255)

                                         aaGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAg                                                                                                         >  2:3693869/1‑150 (MQ=255)

                                             gCCGGGTACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCTCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGcta                                                                                                     <  2:396246/150‑3 (MQ=255)

                                             gCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGa                                                                                                     <  1:2044071/150‑1 (MQ=255)

                                              ccGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGAt                                                                                                    <  2:1569583/150‑1 (MQ=255)

                                                 ggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAc                                                                                                 <  2:4857990/150‑1 (MQ=255)

                                                 ggAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGAc                                                                                                 >  2:4555081/1‑150 (MQ=255)

                                                    aCAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGTTAATTACCTAGACCAATGTTCCGCATAAAGACAACATAGCTTACTTTGGCGCAGGCGATGACCaa                                                                                              >  1:4404666/1‑150 (MQ=255)

                                                     cAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAg                                                                                             <  2:3162083/150‑1 (MQ=255)

                                                      aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                            >  2:3989286/1‑150 (MQ=255)

                                                      aaGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGc                                                                                            >  1:3446005/1‑150 (MQ=255)

                                                       aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGca                                                                                           <  1:3064168/150‑1 (MQ=255)

                                                       aGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGca                                                                                           <  1:2544346/150‑1 (MQ=255)

                                                          tGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACat                                                                                        >  1:4299971/1‑150 (MQ=255)

                                                           gCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACata                                                                                       <  1:2569653/150‑1 (MQ=255)

                                                              tccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATgg                                                                                    <  1:4440648/150‑1 (MQ=255)

                                                               ccatcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgt                                                                                   <  1:3838921/150‑1 (MQ=255)

                                                                 atcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTGTTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGgtgt                                                                                 >  2:2144528/1‑150 (MQ=255)

                                                                  tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTa                                                                                >  2:224597/1‑150 (MQ=255)

                                                                  tcTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAAATTGCTTACTTTTGCGCAGGCGATGTCGAAGCACATATGGTGTa                                                                                >  1:2424931/1‑150 (MQ=255)

                                                                   cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATAATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACATTGGCGCAGGCGATGACGAAGCACATATGGTTTaa                                                                               >  1:1887364/1‑149 (MQ=255)

                                                                   cTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTAAAGTGATCATTACCTAGAACAAATTTACGCAAAAAGACAACATTGCTTACTTTGGCGCACGCGATGACGAAGCACATATGGTGTAc                                                                               >  1:1536619/1‑150 (MQ=255)

                                                                     ggAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCa                                                                             <  2:3210812/150‑1 (MQ=255)

                                                                       aaaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTAAAGTGATCATTACCTAGAACAATGTTACGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACAGATGGTGTACCAGt                                                                           >  2:1028135/1‑150 (MQ=255)

                                                                       aaaaaaCACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATAAAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGt                                                                           >  1:2642072/1‑150 (MQ=255)

                                                                        aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                          <  2:2867531/150‑1 (MQ=255)

                                                                        aaaaaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGtt                                                                          <  2:597032/150‑1 (MQ=255)

                                                                          aaaCACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCGAGAACAATGTTACGCATAAAGACAAAATTGCTTAATTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtct                                                                        >  1:1994539/1‑150 (MQ=255)

                                                                           aaCACATCTGTTTATCAATCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATTAAGACAACATTGCTTACTTTGGCGCTGGCGATGACGAAGCACATTTGGTGTACCAGTtctc                                                                       <  1:4188889/150‑1 (MQ=255)

                                                                           aaCACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTtctc                                                                       <  1:3026473/150‑1 (MQ=255)

                                                                            acacATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCg                                                                      <  2:1272316/150‑1 (MQ=255)

                                                                             cacaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGc                                                                     <  2:1115805/150‑1 (MQ=255)

                                                                             cacaTCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTAGc                                                                     >  2:3620557/1‑150 (MQ=255)

                                                                                 tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACTGTGATCATTACCTAGACCAATGGTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgcc                                                                 <  2:1749611/150‑1 (MQ=255)

                                                                                 tCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgcc                                                                 <  2:1690282/150‑1 (MQ=255)

                                                                                    gATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgcc                                                              <  2:3927189/150‑1 (MQ=255)

                                                                                    gATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTgccgcc                                                              <  2:3975091/150‑1 (MQ=255)

                                                                                      ttATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCAc                                                            >  2:797967/1‑150 (MQ=255)

                                                                                       tATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACt                                                           <  1:2857767/150‑1 (MQ=255)

                                                                                        aTCAAACTGTTACGGTCGAGTATTTATAACGTTTCGCCAGGTACAGTGATCAATACCTAGACCAATGTTCCGCATAAAGACAACAATTCTTAAATTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTATCGCTGCCGCCCCTg                                                          >  1:1480606/1‑150 (MQ=255)

                                                                                          cAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTAACAGTTCTCGCTGCCGCCACTGGt                                                        >  2:875323/1‑150 (MQ=255)

                                                                                          cAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGt                                                        >  1:3410195/1‑150 (MQ=255)

                                                                                            aaCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTTCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgc                                                      >  2:1529545/1‑150 (MQ=255)

                                                                                             aCTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTAAACTGATCATTACCTATACCAATTTTCCGCAAAAAGAAAACCTTGCTTACTTTTGCGCAGGCGATGACGAAGCACAAATGGTGTAACAGTTATAGCAGCCCCCAcatgttct                                                     >  1:3213027/1‑143 (MQ=255)

                                                                                              cTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGtgctg                                                    <  2:4334423/150‑1 (MQ=255)

                                                                                                  tACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATg                                                >  2:2958709/1‑150 (MQ=255)

                                                                                                    cGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCTAGACCAATGTTCCGCAAAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCg                                              >  1:891439/1‑150 (MQ=255)

                                                                                                      gTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGt                                            <  2:2047113/150‑1 (MQ=255)

                                                                                                         gATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCa                                         >  2:3264611/1‑150 (MQ=255)

                                                                                                          attattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaa                                        <  2:5178540/150‑1 (MQ=255)

                                                                                                            tattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCacaa                                      >  2:4658596/1‑150 (MQ=255)

                                                                                                            tattGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCaaaa                                      <  1:3665427/150‑1 (MQ=255)

                                                                                                              ttGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAAc                                    >  2:4735240/1‑150 (MQ=255)

                                                                                                                gATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                                  >  2:2501428/1‑150 (MQ=255)

                                                                                                                gATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAg                                  >  2:1854191/1‑150 (MQ=255)

                                                                                                                   aaCGTTGCGCCAGGTACAGTGATCATTACCTAGAACAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGATGCATGCGGTGCAAAAACAGAAc                               <  2:2799964/150‑1 (MQ=255)

                                                                                                                   aaCGTTGCGCCAGGTACAGTGATCATGACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAc                               <  1:94403/150‑1 (MQ=255)

                                                                                                                    aCGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACg                              <  2:4620869/150‑1 (MQ=255)

                                                                                                                     cGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                             >  1:1235673/1‑150 (MQ=255)

                                                                                                                     cGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGt                             >  2:992888/1‑150 (MQ=255)

                                                                                                                      gTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGtt                            <  2:5026143/150‑1 (MQ=255)

                                                                                                                       ttGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTg                           <  2:4157558/150‑1 (MQ=255)

                                                                                                                          cgcCAGGTACAGTGGTCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAC‑TTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgc                       >  2:1433131/1‑150 (MQ=255)

                                                                                                                           gcCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGgc                       <  1:3542281/150‑1 (MQ=255)

                                                                                                                               ggTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt                   >  2:226388/1‑150 (MQ=255)

                                                                                                                               ggTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCtt                   >  2:436312/1‑150 (MQ=255)

                                                                                                                                gTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCttt                  <  2:4049005/150‑1 (MQ=255)

                                                                                                                                   cAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTtgtg               <  1:2799498/150‑1 (MQ=255)

                                                                                                                                       gATCATTACCTAGACCAATGTTCCGCATAAAGACAACAGTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTg           <  2:1564890/150‑1 (MQ=255)

                                                                                                                                          cATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgc        <  1:4678218/150‑1 (MQ=255)

                                                                                                                                           aTTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGgcg       >  1:302580/1‑150 (MQ=255)

TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAAA  >  CP009273/1365138‑1365431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA  >  CP009273/1365134‑1365430

AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACACTGATCATTACCT                                                                                                                                                     >  SRR19973083.4667147/1‑150 (MQ=60)

AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAAAGTTTCGCCAGGTACAGTGATCATTACCT                                                                                                                                                     >  SRR19973083.4081932/1‑150 (MQ=60)

AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAATATTGATAACGTTGCGCCAGGTACAGTGATCATTACCT                                                                                                                                                     >  SRR19973083.4666861/1‑150 (MQ=60)

 AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                    <  SRR19973083.2235536/150‑1 (MQ=60)

 AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                    >  SRR19973083.4098151/1‑150 (MQ=60)

 AGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTA                                                                                                                                                    <  SRR19973083.4830273/150‑1 (MQ=60)

    TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAC                                                                                                                                                 >  SRR19973083.3410381/1‑150 (MQ=60)

    TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAC                                                                                                                                                 >  SRR19973083.699854/1‑150 (MQ=60)

    TGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGACTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGAC                                                                                                                                                 >  SRR19973083.699872/1‑150 (MQ=60)

     GCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACC                                                                                                                                                <  SRR19973083.3743639/150‑1 (MQ=60)

       TGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTAACGAGACCAA                                                                                                                                              >  SRR19973083.49425/1‑150 (MQ=60)

           TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGTAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                          <  SRR19973083.3738148/150‑1 (MQ=60)

           TATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTT                                                                                                                                          <  SRR19973083.3738150/150‑1 (MQ=60)

             TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTAC                                                                                                                                        >  SRR19973083.658657/1‑150 (MQ=60)

             TGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCC                                                                                                                                        >  SRR19973083.659353/1‑150 (MQ=60)

                CCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCA                                                                                                                                     <  SRR19973083.3747911/150‑1 (MQ=60)

                    CTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAA                                                                                                                                 >  SRR19973083.3426980/1‑150 (MQ=60)

                     TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAG                                                                                                                                <  SRR19973083.2451215/150‑1 (MQ=60)

                     TGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAG                                                                                                                                >  SRR19973083.1567955/1‑150 (MQ=60)

                      GGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGNTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTATAACAATGTTACGCATAAAGA                                                                                                                               >  SRR19973083.4806850/1‑150 (MQ=60)

                      GGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGA                                                                                                                               >  SRR19973083.4285310/1‑150 (MQ=60)

                         TGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAA                                                                                                                            <  SRR19973083.4524676/150‑1 (MQ=60)

                         TGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAA                                                                                                                            <  SRR19973083.1926620/150‑1 (MQ=60)

                          GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAC                                                                                                                           <  SRR19973083.2586299/150‑1 (MQ=60)

                          GCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAC                                                                                                                           <  SRR19973083.2586304/150‑1 (MQ=60)

                           CCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACA                                                                                                                          <  SRR19973083.782787/150‑1 (MQ=60)

                            CGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACAT                                                                                                                         <  SRR19973083.4450512/150‑1 (MQ=60)

                             GTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATT                                                                                                                        >  SRR19973083.5035355/1‑150 (MQ=60)

                               TGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGC                                                                                                                      >  SRR19973083.341904/1‑150 (MQ=60)

                                GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCT                                                                                                                     >  SRR19973083.3047314/1‑150 (MQ=60)

                                GGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATAACAAGACCAATGTTACGCATAAAGACAACATTGCT                                                                                                                     >  SRR19973083.1371401/1‑150 (MQ=60)

                                   TTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTAC                                                                                                                  <  SRR19973083.2284215/150‑1 (MQ=60)

                                     TATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTT                                                                                                                <  SRR19973083.1078551/150‑1 (MQ=60)

                                         TGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGC                                                                                                            <  SRR19973083.1036816/150‑1 (MQ=60)

                                            AAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACTTTGGCGCA                                                                                                         >  SRR19973083.133348/1‑150 (MQ=60)

                                            AAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCA                                                                                                         >  SRR19973083.2761788/1‑150 (MQ=60)

                                             AAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAG                                                                                                        <  SRR19973083.1958758/150‑1 (MQ=60)

                                                 GCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGA                                                                                                    <  SRR19973083.2054617/150‑1 (MQ=60)

                                                 GCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGA                                                                                                    <  SRR19973083.2054507/150‑1 (MQ=60)

                                                   CGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCACACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATG                                                                                                  <  SRR19973083.1589744/150‑1 (MQ=60)

                                                    GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGA                                                                                                 <  SRR19973083.97120/150‑1 (MQ=60)

                                                    GGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCACGTACAGTGATCATTACCAAGACCAAAGTTACGCAAAAAGACAACAaatcgtacaatggagcaggcgaaga                                                                                                 >  SRR19973083.15129/1‑125 (MQ=60)

                                                        ACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAAAGTTTCGCCAGGTACAGTGTTAATTACCTAGACCAATGTTCCGCATAAAGACAACATAGCTTACTTTGGCGCAGGCGATGACCAA                                                                                             >  SRR19973083.4423831/1‑150 (MQ=60)

                                                          AAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGC                                                                                           >  SRR19973083.3461824/1‑150 (MQ=60)

                                                           AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCA                                                                                          <  SRR19973083.3078856/150‑1 (MQ=60)

                                                           AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCA                                                                                          <  SRR19973083.2556666/150‑1 (MQ=60)

                                                           AGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCA                                                                                          <  SRR19973083.2557229/150‑1 (MQ=60)

                                                              TGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACAT                                                                                       >  SRR19973083.4318685/1‑150 (MQ=60)

                                                               GCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATA                                                                                      <  SRR19973083.2582440/150‑1 (MQ=60)

                                                               GCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATA                                                                                      <  SRR19973083.2582620/150‑1 (MQ=60)

                                                                CATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATAGCTTACTTTGGCGCAGGCGATTACGAAGCACATAT                                                                                     >  SRR19973083.3764338/1‑150 (MQ=60)

                                                                 ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAAAGTTCCGCATAAAGACAACAATGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                    >  SRR19973083.1163870/1‑150 (MQ=60)

                                                                 ATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAAAGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATG                                                                                    >  SRR19973083.4504912/1‑150 (MQ=60)

                                                                  TCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGG                                                                                   <  SRR19973083.4459959/150‑1 (MQ=60)

                                                                   CCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGT                                                                                  <  SRR19973083.3856143/150‑1 (MQ=60)

                                                                   CCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGT                                                                                  <  SRR19973083.1734972/150‑1 (MQ=60)

                                                                      TCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAAAATTGCTTACTTTTGCGCAGGCGATGTCGAAGCACATATGGTGTA                                                                               >  SRR19973083.2437375/1‑150 (MQ=60)

                                                                       CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTTCGCCAGGTACAGTGATAATTACCTAGACCAATGTTACGCATAAAGACAACATTGCTTACATTGGCGCAGGCGATGACGAAGCACATATGGTttaa                                                                              >  SRR19973083.1896935/1‑146 (MQ=60)

                                                                       CTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTAAAGTGATCATTACCTAGAACAAATTTACGCAAAAAGACAACATTGCTTACTTTGGCGCACGCGATGACGAAGCACATATGGTGTAC                                                                              >  SRR19973083.1544354/1‑150 (MQ=60)

                                                                          GAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCATTAACGAGACCAATGTTCCGCATAAAGACCACAATGCTTACTTTTGCGCAGGCGATGACGAAGCACAAAAGGTGTACCAG                                                                           >  SRR19973083.935616/1‑150 (MQ=60)

                                                                          GAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGAAAACATTGCTTACTTTGGCGCAGGCGATGAAGAAGCACATATGGTGTACCAG                                                                           >  SRR19973083.4358895/1‑150 (MQ=60)

                                                                           AAAAAACACATCTGATTATCAAACTGTTACGGTCGAGTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAATAAAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGT                                                                          >  SRR19973083.2655342/1‑150 (MQ=60)

                                                                             AAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTC                                                                        >  SRR19973083.4553514/1‑150 (MQ=60)

                                                                              AAACACATCTGATTATCAAACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTACAGTGATCATTACCGAGAACAATGTTACGCATAAAGACAAAATTGCTTAATTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCT                                                                       >  SRR19973083.2004737/1‑150 (MQ=60)

                                                                               AACACATCTGTTTATCAATCTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATTTTCCGCATTAAGACAACATTGCTTACTTTGGCGCTGGCGATGACGAAGCACATTTGGTGTACCAGTTCTC                                                                      <  SRR19973083.4207324/150‑1 (MQ=60)

                                                                               AACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTC                                                                      <  SRR19973083.3041000/150‑1 (MQ=60)

                                                                               AACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTC                                                                      <  SRR19973083.3041033/150‑1 (MQ=60)

                                                                                  ACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCT                                                                   <  SRR19973083.1276587/150‑1 (MQ=60)

                                                                                         ATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCA                                                            <  SRR19973083.1917107/150‑1 (MQ=60)

                                                                                          TTATCAAACTGTTACGGTCGATTATTTATAACGTTTCGCCAGGTACAGTGATCAATAACTAGACCAAAGTTACGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATTACGAAGCACATATGGTGTACCAGTTATCGCTGCCGCCAC                                                           >  SRR19973083.1952845/1‑150 (MQ=60)

                                                                                          TTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCAC                                                           <  SRR19973083.1560077/150‑1 (MQ=60)

                                                                                           TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                          <  SRR19973083.2871857/150‑1 (MQ=60)

                                                                                           TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                          <  SRR19973083.3861697/150‑1 (MQ=60)

                                                                                           TATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACT                                                          <  SRR19973083.244641/150‑1 (MQ=60)

                                                                                            ATCAAACTGTTACGGTCGAGTATTTATAACGTTTCGCCAGGTACAGTGATCAATACCTAGACCAATGTTCCGCATAAAGACAACAATTCTTAAATTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTATCGCTGCCGCCCCTG                                                         >  SRR19973083.1488075/1‑150 (MQ=60)

                                                                                              CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCCGGTACAGTGATCATTACCTAGACCAATTTTACGCCTAAAGACAACATTGCTTACATTTGCGCAGGCGATGACGAATCACATATGGTGTACCCGTTCTCGCTGCCCCCACAGGT                                                       >  SRR19973083.3588176/1‑150 (MQ=60)

                                                                                              CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGT                                                       >  SRR19973083.3425938/1‑150 (MQ=60)

                                                                                              CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGT                                                       <  SRR19973083.3365460/150‑1 (MQ=60)

                                                                                              CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCAAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAATCACATATGGTGTACCAGTTCTCGCTGCCGCCACAGGT                                                       >  SRR19973083.782815/1‑150 (MQ=60)

                                                                                              CAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTAACTAGACCAATGTTCCGCATAAAGACAACAATTCTTACTTTGGCGCAGGCGATGACGAAGCACATAAGGTGTACCAGTTCTAGCTGCCGCCACAGGT                                                       >  SRR19973083.3705396/1‑150 (MQ=60)

                                                                                                 ACTGTTACGGTCGATTATTTATAACGTTGCGCCAGGTAAACTGATCATTACCTATACCAATTTTCCGCAAAAAGAAAACCTTGCTTACTTTTGCGCAGGCGATGACGAAGCACAAATGGTGTAACAGTTatagcagcccccacatgttct                                                    >  SRR19973083.3228266/1‑129 (MQ=60)

                                                                                                       ACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGC                                              <  SRR19973083.2492049/150‑1 (MQ=60)

                                                                                                        CGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTAGCTGCCGCCACTGGTGCTGCATGCG                                             >  SRR19973083.3506863/1‑150 (MQ=60)

                                                                                                        CGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCAATACCTAGACCAATGTTCCGCAAAAAGACAACATTGCTTACTTTTGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCG                                             >  SRR19973083.895656/1‑150 (MQ=60)

                                                                                                         GGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGG                                            >  SRR19973083.775073/1‑150 (MQ=60)

                                                                                                         GGTCGATCATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGG                                            <  SRR19973083.4777589/150‑1 (MQ=60)

                                                                                                               TTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAA                                      >  SRR19973083.4562354/1‑150 (MQ=60)

                                                                                                                TATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAA                                     <  SRR19973083.1072487/150‑1 (MQ=60)

                                                                                                                TATTGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAA                                     <  SRR19973083.3682168/150‑1 (MQ=60)

                                                                                                                   TGATAACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACA                                  <  SRR19973083.1524134/150‑1 (MQ=60)

                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973083.95158/150‑1 (MQ=60)

                                                                                                                       AACGTTGCGCCAGGTACAGTGATCATGACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAAC                              <  SRR19973083.95044/150‑1 (MQ=60)

                                                                                                                         CGTTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGT                            >  SRR19973083.1241726/1‑150 (MQ=60)

                                                                                                                           TTGCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTG                          <  SRR19973083.2035389/150‑1 (MQ=60)

                                                                                                                             GCGCCAGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAG                        <  SRR19973083.4874170/150‑1 (MQ=60)

                                                                                                                               GCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGC                      <  SRR19973083.3558545/150‑1 (MQ=60)

                                                                                                                                  AGGTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCT                   >  SRR19973083.3676787/1‑150 (MQ=60)

                                                                                                                                    GTACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTT                 <  SRR19973083.3190375/150‑1 (MQ=60)

                                                                                                                                      ACAGTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGT               >  SRR19973083.3461575/1‑150 (MQ=60)

                                                                                                                                       CAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTG              <  SRR19973083.2813433/150‑1 (MQ=60)

                                                                                                                                       CAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTG              <  SRR19973083.2813395/150‑1 (MQ=60)

                                                                                                                                        AGTGATCATTACCTAGACCAATGATCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGC             >  SRR19973083.88092/1‑150 (MQ=60)

                                                                                                                                         GTGATCATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCG            >  SRR19973083.4407133/1‑150 (MQ=60)

                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGC       <  SRR19973083.1434512/150‑1 (MQ=60)

                                                                                                                                              CATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGC       <  SRR19973083.4698320/150‑1 (MQ=60)

                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG      >  SRR19973083.304204/1‑150 (MQ=60)

                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG      >  SRR19973083.304134/1‑150 (MQ=60)

                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG      >  SRR19973083.3028314/1‑150 (MQ=60)

                                                                                                                                               ATTACCTAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCG      >  SRR19973083.2929979/1‑150 (MQ=60)

                                                                                                                                                TTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGC     >  SRR19973083.4085582/1‑150 (MQ=60)

                                                                                                                                                   CCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAGCGTTGAGGCGCTTTGTGCGTGGGCGCAAA  <  SRR19973083.338152/150‑1 (MQ=60)

AAGGTGCTGAGTATGTCCGCCTGGATGCCGTTGGCTTTATGTGGAAAGAGCCGGGAACAAGCTGCATCCATCTGGAAAAAACACATCTGATTATCAAACTGTTACGGTCGATTATTGATAACGTTGCGCCAGGTACAGTGATCATTACCGAGACCAATGTTCCGCATAAAGACAACATTGCTTACTTTGGCGCAGGCGATGACGAAGCACATATGGTGTACCAGTTCTCGCTGCCGCCACTGGTGCTGCATGCGGTGCAAAAACAGAACGTTGAGGCGCTTTGTGCGTGGGCGCAAA  >  CP009273/1365134‑1365430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`