Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F86 I0 R1	593	297.4	9515568	94.4%	8982696	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	235,036	G→T	23.1%	S63Y (TCC→TAC)	ykfM ←	lethality reduction protein, putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	235,036	0	G	T	23.1%	129.2 / 4.8	77	S63Y (TCC→TAC)	ykfM	lethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (40/14); new base T (2/20); total (42/35)
Fisher's exact test for biased strand distribution p-value = 2.41e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAA  >  CP009273/234889‑235180

gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                                <  1:1997792/150‑1 (MQ=255)

       aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACa                                                                                                                                         >  1:3824134/1‑150 (MQ=255)

       aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAATGAAAACGACa                                                                                                                                         >  1:3822867/1‑150 (MQ=255)

        aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGAAAt                                                                                                                                        >  1:3925225/1‑150 (MQ=255)

           tGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGTTTAATAATGATTTTATTATAGTAATTAAAATTAAATTAATATTTAAAAAAGAAAAAGACAGGaa                                                                                                                                     >  1:4432166/1‑150 (MQ=255)

                     aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAAATATTTTATTATAGTAAGTAAAATTAAATGAATAATTAAAAATGAAAAAGACATTAAAACGAAGcac                                                                                                                           >  1:4636579/1‑150 (MQ=255)

                     aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAACGAAGcac                                                                                                                           >  2:768390/1‑150 (MQ=255)

                       aGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATTATTTTATTATATTAAGTAAAATTAAAATAATATTTAAAAATGAAAAACAAAAGAAAACAAAGCACAg                                                                                                                         >  1:586973/1‑150 (MQ=255)

                         atATTAGTAAAAATATAATTTTTAGCTTTATTTTTTTGTCTGTGGCTAAATTTACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCTTAATTAAAATTAAATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                                       <  2:1373169/149‑1 (MQ=255)

                          tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAATAAAGATTTTATTATCGTAAGTAAAATTAAAAGAATATTTAAAAAGGAAAAAGACAAGAAAAAGAATCACAGAAt                                                                                                                      >  1:308908/1‑150 (MQ=255)

                             tattAAAAATATATGTGTTCGCTTTTTTGTTTTGTCTGTGGCTAATTTGACTACCTTGTTTTTTTTTATTAAGAATGTTTTTTTTATCGTATTTAAATTTACATTAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                                                   <  1:3149441/147‑1 (MQ=255)

                             tAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAACaa                                                                                                                   >  2:2023860/1‑150 (MQ=255)

                              aGTAAAAATATAAGAGTTCGCTGTTTTTTTATTTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                                  <  2:1387707/150‑1 (MQ=255)

                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAAATATTTTATTATAGTAAGTAAAAATAAAAGAATATTTTAAAAGTAAAAAGACAGGAAAAAGAAGCACACaaaaaaaca                                                                                                                >  1:3324496/1‑143 (MQ=255)

                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTTTTATAGTAAGTAAAAATAAATTAATAATTAAAAAAGAAAAACACATGAAACCCAAGCCCACaaaaaaaaa                                                                                                                >  1:3324330/1‑143 (MQ=255)

                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAAAGATTTTATTAAAGTAATTAAAATAAAATTAAAAATTAAAAAAGAAAAAGAAAAGAAACCCCAGCACACAATAAACaa                                                                                                                >  1:3324376/1‑149 (MQ=255)

                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATaaaaca                                                                                                                >  2:4026525/1‑147 (MQ=255)

                                 aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATTAAACCGAAGCACAGAATAAACaat                                                                                                               >  2:515249/1‑148 (MQ=255)

                                     atatAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAATAAAATTTAAAAAGGAAAAACACAATAAACCCAAGCACACAATAAACAAtcaac                                                                                                           >  2:2040779/1‑147 (MQ=255)

                                      tataATATTTAGCTGTTTTTTTATGTCTGTGGCTAATTTGAATACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAAGAAAAATTACATGAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                                                          <  1:3563025/150‑1 (MQ=255)

                                       ataAGTGTTAGCTGTTTTGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                                         <  2:2188873/150‑1 (MQ=255)

                                                  ttgttttgtttTGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATCGTATGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                              <  2:918805/149‑1 (MQ=255)

                                                  cTGTTTTTTTATGTCTGTGGCGAAATTTACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCGTAAGTAAATTTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                              <  2:918818/150‑1 (MQ=255)

                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAATCTCCAATCATAAAAAAtt                                                                                             >  1:3096015/1‑150 (MQ=255)

                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATTAAAACGAAGCACAGAATCAACAATCTCCAAaaataaaataat                                                                                             >  1:3096028/1‑148 (MQ=255)

                                                     tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACCGAATAAACATTCTCCAATCATAAAATAttta                                                                                           >  2:1216259/1‑149 (MQ=255)

                                                     tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGTAACCGAAGCACAGAATCAAAATTATACAAACATAAAATAtttg                                                                                           >  1:2245048/1‑149 (MQ=255)

                                                     atttGTTATTTCTGTGGCGAAATTTAATACATTCTTTTTTTTTATTAAGAATTATTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                           <  2:3702073/148‑1 (MQ=255)

                                                        tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATAAAAGAATATTTAAAAATTAAAACGACAATAAACCGAAGCACACAATCAAAAATATCCAAGCAAAAAATAattaccg                                                                                        >  1:3265384/1‑146 (MQ=255)

                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTATAAATATTTTTTTATAGTAAATAAAATTACAATAAAATTTAAAAATGAAAAAGACATGAAAACGAAACACAGAATAAAAAATATACAAACAAAAAAAATTTAAGTgg                                                                                      >  1:1391296/1‑150 (MQ=255)

                                                           tATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAAATACATGAATATTTAAAAATGAAAACGACAAGAAACCCAAACACAGAAAAAAAATTATACCATCAAAAAAAAATTAAGTGGa                                                                                     >  1:543655/1‑150 (MQ=255)

                                                                gtgggtcGAATTTGACTACTTTCGTTTTTTTGTTTATTATTTATTTTATTATCGTAATTAAATTTAAATTAATATTAAAAATTTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                                <  1:3017023/144‑1 (MQ=255)

                                                                  gtgtctaaaTTTAATACATTTTTTTTTTTGATTAATATTTATTTTATAATCTTAATAAAAATTACATTAATATTTAAAAAGTAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                              <  1:4010823/144‑1 (MQ=255)

                                                                  gtgGCTAAATTTACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                              <  1:4313087/150‑1 (MQ=255)

                                                                   tgGCTAAATTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                                             <  2:3942202/150‑1 (MQ=255)

                                                                                 aCCTTCTTTTTTTTTATTAATATTTTTTTTTTTATCGTAATTAAAATTACATGAATATTTAAAATGGAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                                               <  1:3698351/150‑1 (MQ=255)

                                                                                 aCCTTCTTTTTTTTTATTAATATTTATTTTATTATCGTAATTAAAATTACATGAATATTAAAAAAGTAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                                               <  1:3698285/150‑1 (MQ=255)

                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                              <  2:3512341/150‑1 (MQ=255)

                                                                                   cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                                                             >  1:2535667/1‑150 (MQ=255)

                                                                                      ggTTTTTTTGATTAACAATGATTTTATTATCTTAATTAAAATTACATGAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                          <  2:1297737/149‑1 (MQ=255)

                                                                                      cGTTTTTTTGATAAATAATGATTTTATTATCGTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                          <  2:1297735/150‑1 (MQ=255)

                                                                                             tttaTTAAGATTTATTTTATTATCGTAATTAAATTTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                                   <  1:736427/147‑1 (MQ=255)

                                                                                              tttttAATAATTTTTTTTTTATCTTAATTAAAATTAAATTAATTTTTAAAAAGCAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCgg                                                  <  1:3255109/147‑1 (MQ=255)

                                                                                                 ttAATAATTATTTTATTATCTTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                               <  1:374860/150‑1 (MQ=255)

                                                                                                 ttAATAATGATTTTATTATCTTATTTAAAATTAATTTAATATTTAAAATTGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                               <  1:980298/150‑1 (MQ=255)

                                                                                                 ttAAGATTTATTTTTTTTTCGTAAGTAATTTTATTTTATTATTTAAAATGGAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                               <  1:575641/150‑1 (MQ=255)

                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                               >  1:2474432/1‑150 (MQ=255)

                                                                                                        ttaTTTTATTATCTTAATTAAATTTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                                        <  1:768390/148‑1 (MQ=255)

                                                                                                         taTTTTATTATCTAAATAAAAATTAAATTAATATTTAAAAATTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                                       <  1:2435682/149‑1 (MQ=255)

                                                                                                          aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                                      >  2:2229708/1‑150 (MQ=255)

                                                                                                          aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                                      >  2:397689/1‑150 (MQ=255)

                                                                                                          aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTACCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                                      >  2:2230966/1‑150 (MQ=255)

                                                                                                            tttATTATCGTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCTGGTAAAAAACAaagaa                                    <  2:917912/150‑1 (MQ=255)

                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                                    >  2:3454947/1‑150 (MQ=255)

                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                                    >  1:3382775/1‑150 (MQ=255)

                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                                    >  1:3384961/1‑150 (MQ=255)

                                                                                                                 tatCTTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                               <  1:4670497/150‑1 (MQ=255)

                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                               >  2:2378019/1‑150 (MQ=255)

                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                               >  2:4425491/1‑150 (MQ=255)

                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTACCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                               >  2:4423606/1‑150 (MQ=255)

                                                                                                                    cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                            >  2:2645501/1‑150 (MQ=255)

                                                                                                                       aagtaaAATTACATGAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGc                         <  2:2245048/150‑1 (MQ=255)

                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg                       >  1:4701542/1‑150 (MQ=255)

                                                                                                                              aTTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaa                  <  2:4401921/150‑1 (MQ=255)

                                                                                                                               ttAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa                 <  2:3256182/150‑1 (MQ=255)

                                                                                                                                  cATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaata              >  1:4150733/1‑150 (MQ=255)

                                                                                                                                     gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata           >  2:941377/1‑150 (MQ=255)

                                                                                                                                      aaTATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTTTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAatat          <  1:1069691/150‑1 (MQ=255)

                                                                                                                                      aaTATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTTTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAatat          <  1:1068522/150‑1 (MQ=255)

                                                                                                                                      aaTATTAAAAAAGTAAAACGACAAGAAACCGAAGCACAGAATCAACATTTTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAatat          <  1:321496/150‑1 (MQ=255)

                                                                                                                                       atatTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATg         <  2:2310234/150‑1 (MQ=255)

                                                                                                                                        tatTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATgg        >  1:1539009/1‑150 (MQ=255)

                                                                                                                                         atTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAGTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGc       >  1:4640971/1‑150 (MQ=255)

                                                                                                                                            tAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGACAAATAATATGGCCAt    >  2:3734935/1‑150 (MQ=255)

                                                                                                                                            tAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCAt    >  2:4094883/1‑150 (MQ=255)

                                                                                                                                            tAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCAt    >  2:3734934/1‑150 (MQ=255)

                                                                                                                                              aaaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaa  <  2:586973/150‑1 (MQ=255)

GCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAA  >  CP009273/234889‑235180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A