Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 153,472 G→T 87.1% intergenic (‑102/+268) yadN ← / ← folK putative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273153,4720GT87.1% 676.4 / 93.5 252intergenic (‑102/+268)yadN/folKputative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base G (20/13);  new base T (115/104);  total (135/117)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

ATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  CP009273/153324‑153618
                                                                                                                                                    |                                                                                                                                                  
   gCCAGCACAAGACCAGACAGTGCAAAAACTAAATTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                                <  2:3434970/150‑1 (MQ=255)
    ccAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAtt                                                                                                                                               <  1:3546295/150‑1 (MQ=255)
      aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                             >  1:1874378/1‑150 (MQ=255)
      aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTATGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                             <  2:1767892/150‑1 (MQ=255)
        cATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGtt                                                                                                                                           <  2:2114640/150‑1 (MQ=255)
             gACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCa                                                                                                                                      <  2:4158967/150‑1 (MQ=255)
             gACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCa                                                                                                                                      >  2:3908441/1‑150 (MQ=255)
              aCCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCaa                                                                                                                                     >  1:1150898/1‑150 (MQ=255)
                 aGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAAtt                                                                                                                                  >  1:3448061/1‑150 (MQ=255)
                 aGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAAtt                                                                                                                                  >  1:4534556/1‑150 (MQ=255)
                     aGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACa                                                                                                                              >  2:885700/1‑150 (MQ=255)
                          aaaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAt                                                                                                                         >  1:3889492/1‑150 (MQ=255)
                          aaaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAt                                                                                                                         >  1:676846/1‑150 (MQ=255)
                          aaaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAt                                                                                                                         <  2:2848847/150‑1 (MQ=255)
                          aaaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAt                                                                                                                         >  2:661736/1‑150 (MQ=255)
                              ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                     <  1:1769354/150‑1 (MQ=255)
                                 aaTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCAt                                                                                                                  <  2:2645660/150‑1 (MQ=255)
                                       ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAACTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                            >  2:3769224/1‑150 (MQ=255)
                                       ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                            <  2:1824710/150‑1 (MQ=255)
                                       ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                            >  2:150784/1‑150 (MQ=255)
                                        tAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCg                                                                                                           <  1:1515644/150‑1 (MQ=255)
                                           aCATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCt                                                                                                        >  1:3577179/1‑150 (MQ=255)
                                            cATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTa                                                                                                       >  2:1657112/1‑150 (MQ=255)
                                              tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                     >  1:52643/1‑150 (MQ=255)
                                               aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGGTCATTAGGTCGCCTATtg                                                                                                    >  2:2788040/1‑150 (MQ=255)
                                                aaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgt                                                                                                   >  2:2505446/1‑150 (MQ=255)
                                                 aaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                                  <  1:1676921/150‑1 (MQ=255)
                                                   aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                                <  1:2241462/150‑1 (MQ=255)
                                                   aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                                >  1:3638096/1‑150 (MQ=255)
                                                   aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtgga                                                                                                >  1:4425838/1‑148 (MQ=255)
                                                   aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                                >  2:126995/1‑150 (MQ=255)
                                                    tCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCAc                                                                                               <  2:4471382/150‑1 (MQ=255)
                                                     ccTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACt                                                                                              >  2:3851694/1‑150 (MQ=255)
                                                       tttAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACttt                                                                                            <  1:4224032/150‑1 (MQ=255)
                                                         taataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAg                                                                                          <  1:1657725/150‑1 (MQ=255)
                                                          aataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                         >  1:1168708/1‑150 (MQ=255)
                                                            taaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAg                                                                                       <  1:3341737/150‑1 (MQ=255)
                                                              aaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCt                                                                                     >  1:2950357/1‑150 (MQ=255)
                                                                aTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCttt                                                                                   >  1:872209/1‑150 (MQ=255)
                                                                 ttCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCtttt                                                                                  <  1:1962291/150‑1 (MQ=255)
                                                                  tCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTg                                                                                 >  2:3520604/1‑150 (MQ=255)
                                                                  tCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTg                                                                                 <  2:4168094/150‑1 (MQ=255)
                                                                   ccATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTGTTGTGCACTTTAGAAGCTGTTgt                                                                                >  2:2771921/1‑149 (MQ=255)
                                                                        aGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaa                                                                           <  1:1502776/150‑1 (MQ=255)
                                                                           cAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaatta                                                                        >  2:3942489/1‑150 (MQ=255)
                                                                                tACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAAttt                                                                   >  2:3926980/1‑150 (MQ=255)
                                                                                    tGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACtt                                                               <  2:1464032/150‑1 (MQ=255)
                                                                                    tGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACtt                                                               <  2:831930/150‑1 (MQ=255)
                                                                                    tGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACtt                                                               >  1:4258249/1‑150 (MQ=255)
                                                                                     gTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTa                                                              <  1:3196090/150‑1 (MQ=255)
                                                                                     gTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAAGGAGCAATTATATTTGTTACCCAAATTTACAACCAGTGTTCTTTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTa                                                              <  1:869347/150‑1 (MQ=255)
                                                                                      tttGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTaa                                                             <  1:3407196/150‑1 (MQ=255)
                                                                                       ttGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAt                                                            <  2:3448061/150‑1 (MQ=255)
                                                                                        tGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAtt                                                           <  2:218213/150‑1 (MQ=255)
                                                                                            gaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCaaa                                                       >  1:1334741/1‑150 (MQ=255)
                                                                                               tGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAAtt                                                    >  2:3580316/1‑150 (MQ=255)
                                                                                                gACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTa                                                   >  2:1012187/1‑150 (MQ=255)
                                                                                                 aCTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTaa                                                  >  1:225184/1‑150 (MQ=255)
                                                                                                  cTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAg                                                 <  1:2824521/150‑1 (MQ=255)
                                                                                                     ttCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaa                                              >  2:565177/1‑150 (MQ=255)
                                                                                                       cATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTATGTaaaa                                            >  2:3069388/1‑150 (MQ=255)
                                                                                                       cATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaaa                                            <  2:75215/150‑1 (MQ=255)
                                                                                                        aTGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaaaa                                           <  2:1021861/150‑1 (MQ=255)
                                                                                                          gACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATa                                         >  1:15760/1‑150 (MQ=255)
                                                                                                          gACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATa                                         >  2:3211785/1‑150 (MQ=255)
                                                                                                          gACACAAATAGCAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAATGTGCACTTTAGAAGATTTTGAACAAATAAAATTTACAAAATTCAAAATTAAGTAAAAATa                                         >  2:3211931/1‑150 (MQ=255)
                                                                                                            cacaAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAg                                       <  1:390906/150‑1 (MQ=255)
                                                                                                             acaAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGt                                      >  2:2528436/1‑150 (MQ=255)
                                                                                                             acaAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGt                                      >  2:1229973/1‑150 (MQ=255)
                                                                                                              caAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                     >  2:699689/1‑150 (MQ=255)
                                                                                                              caAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                     >  1:349347/1‑150 (MQ=255)
                                                                                                               aaaTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTc                                    >  1:2070744/1‑150 (MQ=255)
                                                                                                               aaaTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTc                                    >  1:3682987/1‑150 (MQ=255)
                                                                                                               aaaTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTc                                    >  1:884998/1‑150 (MQ=255)
                                                                                                               aaaTAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTc                                    <  1:2603434/150‑1 (MQ=255)
                                                                                                               aaaTACGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTc                                    <  2:263053/150‑1 (MQ=255)
                                                                                                                aaTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTca                                   <  2:272801/150‑1 (MQ=255)
                                                                                                                aaTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTca                                   <  1:661720/150‑1 (MQ=255)
                                                                                                                 atatGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACACAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcac                                  <  2:495182/150‑1 (MQ=255)
                                                                                                                 aTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTAGTTACCCAAATTTACAACCATTGTTCATTACGTCGCCTATTGAGCACTATAGAAGCATTAGAACAAATTAAATTTACTTAATTCAAAATTAAGAAAAAATAAGTacac                                  >  1:2624707/1‑150 (MQ=255)
                                                                                                                 aTAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcac                                  <  2:2759558/150‑1 (MQ=255)
                                                                                                                  tAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcaca                                 >  2:211969/1‑150 (MQ=255)
                                                                                                                  tAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcaca                                 <  1:4320037/150‑1 (MQ=255)
                                                                                                                      agaAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTg                             <  2:2243246/150‑1 (MQ=255)
                                                                                                                      agaAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTg                             >  2:2354288/1‑150 (MQ=255)
                                                                                                                          aCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                         >  2:375639/1‑150 (MQ=255)
                                                                                                                            aaaTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTg                       <  2:3092953/150‑1 (MQ=255)
                                                                                                                              aTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGt                     >  2:3323797/1‑150 (MQ=255)
                                                                                                                              aTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGt                     >  2:1043426/1‑150 (MQ=255)
                                                                                                                                  tAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAgg                 <  2:3063161/150‑1 (MQ=255)
                                                                                                                                    gATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGt               >  2:2044914/1‑150 (MQ=255)
                                                                                                                                     atatTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTCGAAGCTTTTGAACAAATTAAATGTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTa              <  1:3840154/150‑1 (MQ=255)
                                                                                                                                     atatTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTa              <  1:1242495/150‑1 (MQ=255)
                                                                                                                                      tatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGtct             >  2:1276789/1‑148 (MQ=255)
                                                                                                                                      tatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGtct             >  2:4373479/1‑148 (MQ=255)
                                                                                                                                        ttAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATgg           >  2:1689388/1‑150 (MQ=255)
                                                                                                                                          aaTGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGgag         <  1:3071947/150‑1 (MQ=255)
                                                                                                                                            tGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTCTGGAGAt       >  1:2894474/1‑150 (MQ=255)
                                                                                                                                              aGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATgg     <  1:1721234/150‑1 (MQ=255)
                                                                                                                                                cAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGa   <  1:3908499/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  CP009273/153324‑153618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  CP009273/153327‑153618
                                                                                                                                                 |                                                                                                                                                  
GCCATCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAT                                                                                                                                                <  SRR19973080.2770254/150‑1 (MQ=60)
 CCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATT                                                                                                                                               <  SRR19973080.4360312/150‑1 (MQ=60)
 CCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATT                                                                                                                                               <  SRR19973080.3565911/150‑1 (MQ=60)
 CCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATT                                                                                                                                               >  SRR19973080.3526806/1‑150 (MQ=60)
  CAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTT                                                                                                                                              <  SRR19973080.4302690/150‑1 (MQ=60)
   AGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                             <  SRR19973080.3128309/150‑1 (MQ=60)
   AGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                             >  SRR19973080.1884834/1‑150 (MQ=60)
      ATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTA                                                                                                                                          >  SRR19973080.2617719/1‑150 (MQ=60)
        AAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACC                                                                                                                                        >  SRR19973080.1247920/1‑150 (MQ=60)
         AGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCC                                                                                                                                       >  SRR19973080.877063/1‑150 (MQ=60)
           ACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAA                                                                                                                                     >  SRR19973080.1157682/1‑150 (MQ=60)
              AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                  >  SRR19973080.3466899/1‑150 (MQ=60)
              AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                  >  SRR19973080.3466982/1‑150 (MQ=60)
              AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                  >  SRR19973080.4558963/1‑150 (MQ=60)
              AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                  >  SRR19973080.4558767/1‑150 (MQ=60)
              AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                  >  SRR19973080.3466887/1‑150 (MQ=60)
                  AGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACA                                                                                                                              >  SRR19973080.2109340/1‑150 (MQ=60)
                  AGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTAAA                                                                                                                              >  SRR19973080.126799/1‑150 (MQ=60)
                       AAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAT                                                                                                                         >  SRR19973080.680865/1‑150 (MQ=60)
                       AAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAT                                                                                                                         >  SRR19973080.2902012/1‑150 (MQ=60)
                       AAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAT                                                                                                                         >  SRR19973080.3910929/1‑150 (MQ=60)
                           CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                     <  SRR19973080.1779512/150‑1 (MQ=60)
                           CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                     <  SRR19973080.1779517/150‑1 (MQ=60)
                                     TAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCG                                                                                                           <  SRR19973080.1523522/150‑1 (MQ=60)
                                     TAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCG                                                                                                           <  SRR19973080.1524328/150‑1 (MQ=60)
                                        ACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCT                                                                                                        >  SRR19973080.3596968/1‑150 (MQ=60)
                                        ACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCT                                                                                                        >  SRR19973080.3597377/1‑150 (MQ=60)
                                        ACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACAAAAATTTACAACCATAGTTCATTAGTTCGCCT                                                                                                        >  SRR19973080.3596947/1‑150 (MQ=60)
                                           TAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATT                                                                                                     >  SRR19973080.53171/1‑150 (MQ=60)
                                           TAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATT                                                                                                     >  SRR19973080.53023/1‑150 (MQ=60)
                                            AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                    >  SRR19973080.1758869/1‑150 (MQ=60)
                                            AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                    >  SRR19973080.1758019/1‑150 (MQ=60)
                                             AAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGT                                                                                                   >  SRR19973080.779709/1‑150 (MQ=60)
                                              AAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTG                                                                                                  <  SRR19973080.1686712/150‑1 (MQ=60)
                                                ATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCA                                                                                                >  SRR19973080.3658189/1‑150 (MQ=60)
                                                ATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCA                                                                                                <  SRR19973080.2253758/150‑1 (MQ=60)
                                                ATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCA                                                                                                <  SRR19973080.2253799/150‑1 (MQ=60)
                                                ATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGGA                                                                                                >  SRR19973080.4449893/1‑150 (MQ=60)
                                                  CCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACT                                                                                              >  SRR19973080.1218032/1‑150 (MQ=60)
                                                    TTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTT                                                                                            <  SRR19973080.4247022/150‑1 (MQ=60)
                                                    TTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTT                                                                                            <  SRR19973080.4247023/150‑1 (MQ=60)
                                                     TTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTA                                                                                           >  SRR19973080.88971/1‑150 (MQ=60)
                                                      TAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAG                                                                                          <  SRR19973080.1667423/150‑1 (MQ=60)
                                                      TAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAG                                                                                          <  SRR19973080.1667413/150‑1 (MQ=60)
                                                      TAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAG                                                                                          <  SRR19973080.1667433/150‑1 (MQ=60)
                                                       CATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTGCGATATTAATGAGCACTTATATGTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGA                                                                                         >  SRR19973080.1175533/1‑150 (MQ=60)
                                                       AATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGA                                                                                         >  SRR19973080.1175662/1‑150 (MQ=60)
                                                        ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                        >  SRR19973080.4265290/1‑150 (MQ=60)
                                                         TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                       <  SRR19973080.3414521/150‑1 (MQ=60)
                                                         TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                       <  SRR19973080.3885024/150‑1 (MQ=60)
                                                         TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                       <  SRR19973080.3359927/150‑1 (MQ=60)
                                                         TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                       <  SRR19973080.3359929/150‑1 (MQ=60)
                                                           AAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCT                                                                                     >  SRR19973080.2966361/1‑150 (MQ=60)
                                                           AAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCT                                                                                     >  SRR19973080.2966250/1‑150 (MQ=60)
                                                             ATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTT                                                                                   >  SRR19973080.877529/1‑150 (MQ=60)
                                                             ATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTT                                                                                   >  SRR19973080.877711/1‑150 (MQ=60)
                                                              TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                  <  SRR19973080.1973420/150‑1 (MQ=60)
                                                                 CATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAA                                                                               >  SRR19973080.2371321/1‑150 (MQ=60)
                                                                   TTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACA                                                                             <  SRR19973080.1567362/150‑1 (MQ=60)
                                                                    TAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAA                                                                            >  SRR19973080.1664800/1‑150 (MQ=60)
                                                                     AGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAA                                                                           <  SRR19973080.1511330/150‑1 (MQ=60)
                                                                             TACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTT                                                                   <  SRR19973080.276472/150‑1 (MQ=60)
                                                                              ACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTA                                                                  >  SRR19973080.3683798/1‑150 (MQ=60)
                                                                                 TGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTT                                                               >  SRR19973080.4281475/1‑150 (MQ=60)
                                                                                  GTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTA                                                              <  SRR19973080.3213727/150‑1 (MQ=60)
                                                                                  GTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTA                                                              <  SRR19973080.874634/150‑1 (MQ=60)
                                                                                  GTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTA                                                              <  SRR19973080.3744256/150‑1 (MQ=60)
                                                                                  GTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTA                                                              <  SRR19973080.3152959/150‑1 (MQ=60)
                                                                                  GTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAAGGAGCAATTATATTTGTTACCCAAATTTACAACCAGTGTTCTTTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTA                                                              <  SRR19973080.874646/150‑1 (MQ=60)
                                                                                   TTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAA                                                             <  SRR19973080.3425643/150‑1 (MQ=60)
                                                                                    TTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAT                                                            >  SRR19973080.627812/1‑150 (MQ=60)
                                                                                     TGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATT                                                           <  SRR19973080.3042645/150‑1 (MQ=60)
                                                                                         GAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAA                                                       >  SRR19973080.1342789/1‑150 (MQ=60)
                                                                                            TGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACTACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATT                                                    <  SRR19973080.4133230/150‑1 (MQ=60)
                                                                                              ACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAA                                                  >  SRR19973080.226795/1‑150 (MQ=60)
                                                                                               CTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAG                                                 <  SRR19973080.2840054/150‑1 (MQ=60)
                                                                                                  TTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAA                                              <  SRR19973080.295446/150‑1 (MQ=60)
                                                                                                      TGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAAT                                          <  SRR19973080.3624928/150‑1 (MQ=60)
                                                                                                       GACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATA                                         >  SRR19973080.15949/1‑150 (MQ=60)
                                                                                                         CACAAATATGAGAAACAAATGTTAGATATTAATGAGCACTTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTTATTCAAAATTAAGTAAAAATAAG                                       <  SRR19973080.393164/150‑1 (MQ=60)
                                                                                                         CACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAG                                       <  SRR19973080.393172/150‑1 (MQ=60)
                                                                                                           CAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTT                                     >  SRR19973080.351447/1‑150 (MQ=60)
                                                                                                           CAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTT                                     >  SRR19973080.351398/1‑150 (MQ=60)
                                                                                                            AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTC                                    >  SRR19973080.3703241/1‑150 (MQ=60)
                                                                                                            AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTC                                    >  SRR19973080.890422/1‑150 (MQ=60)
                                                                                                            AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTC                                    >  SRR19973080.1885311/1‑150 (MQ=60)
                                                                                                            AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTC                                    >  SRR19973080.1885771/1‑150 (MQ=60)
                                                                                                            AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTC                                    >  SRR19973080.2082481/1‑150 (MQ=60)
                                                                                                            AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTC                                    <  SRR19973080.2617580/150‑1 (MQ=60)
                                                                                                             AATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCA                                   <  SRR19973080.665699/150‑1 (MQ=60)
                                                                                                             AATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCA                                   <  SRR19973080.665683/150‑1 (MQ=60)
                                                                                                              ATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTAGTTACCCAAATTTACAACCATTGTTCATTACGTCGCCTATTGAGCACTATAGAAGCATTAGAACAAATTAAATTTACTTAATTCAAAATTAAGAAAAAATAAGTACAC                                  >  SRR19973080.2638924/1‑150 (MQ=60)
                                                                                                               TAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACA                                 <  SRR19973080.4343638/150‑1 (MQ=60)
                                                                                                                    GAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGC                            >  SRR19973080.1456791/1‑150 (MQ=60)
                                                                                                                    GAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGC                            >  SRR19973080.3752389/1‑150 (MQ=60)
                                                                                                                      AACAAAGGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAA                          >  SRR19973080.1556844/1‑150 (MQ=60)
                                                                                                                         AAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCACTTG                       >  SRR19973080.2019012/1‑150 (MQ=60)
                                                                                                                          AATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGG                      <  SRR19973080.663090/150‑1 (MQ=60)
                                                                                                                           ATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGT                     >  SRR19973080.3305382/1‑150 (MQ=60)
                                                                                                                             GTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTA                   <  SRR19973080.891129/150‑1 (MQ=60)
                                                                                                                               TAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGG                 >  SRR19973080.1510439/1‑150 (MQ=60)
                                                                                                                                AGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGG                >  SRR19973080.2168719/1‑150 (MQ=60)
                                                                                                                                  ATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTA              >  SRR19973080.3443601/1‑150 (MQ=60)
                                                                                                                                  ATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTCGAAGCTTTTGAACAAATTAAATGTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTA              <  SRR19973080.3861248/150‑1 (MQ=60)
                                                                                                                                  ATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTA              <  SRR19973080.1250018/150‑1 (MQ=60)
                                                                                                                                       AATGATCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAG         <  SRR19973080.4500150/150‑1 (MQ=60)
                                                                                                                                       AATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAG         <  SRR19973080.3088692/150‑1 (MQ=60)
                                                                                                                                         TGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTCTGGAGAT       >  SRR19973080.2910292/1‑150 (MQ=60)
                                                                                                                                         TGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGAT       <  SRR19973080.2464357/150‑1 (MQ=60)
                                                                                                                                           AGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGG     <  SRR19973080.1731199/150‑1 (MQ=60)
                                                                                                                                           AGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGG     <  SRR19973080.1731200/150‑1 (MQ=60)
                                                                                                                                             CAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGA   <  SRR19973080.3930066/150‑1 (MQ=60)
                                                                                                                                             CAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGA   <  SRR19973080.3930045/150‑1 (MQ=60)
                                                                                                                                             CAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGA   <  SRR19973080.3930007/150‑1 (MQ=60)
                                                                                                                                              AATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  <  SRR19973080.4309900/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                  
GCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  CP009273/153327‑153618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑