Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 153,472 G→T 94.8% intergenic (‑102/+268) yadN ← / ← folK putative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273153,4720GT94.8% 779.0 / 31.2 248intergenic (‑102/+268)yadN/folKputative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base G (9/4);  new base T (119/115);  total (129/119)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 251 total aligned reads displayed.

TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGG  >  CP009273/153325‑153616
                                                                                                                                                   |                                                                                                                                                
tCGCCATCATAAGACCAGACAGTGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTat                                                                                                                                                <  2:1168279/150‑1 (MQ=255)
 cGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATtata                                                                                                                                               <  1:822242/150‑1 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                              >  2:4370781/1‑150 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                              >  2:591585/1‑150 (MQ=255)
   ccAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAtt                                                                                                                                             >  2:3321842/1‑150 (MQ=255)
    cAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAttt                                                                                                                                            >  1:3548225/1‑150 (MQ=255)
    cAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAttt                                                                                                                                            <  1:4090235/150‑1 (MQ=255)
     aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                           >  2:2899477/1‑150 (MQ=255)
     aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                           >  2:2900355/1‑150 (MQ=255)
     aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                           >  1:2268249/1‑150 (MQ=255)
        aTAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTa                                                                                                                                        >  2:2769570/1‑150 (MQ=255)
             aCCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCaa                                                                                                                                   <  2:129098/150‑1 (MQ=255)
                aGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAAtt                                                                                                                                >  1:4010002/1‑150 (MQ=255)
                aGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAAtt                                                                                                                                >  2:3933524/1‑150 (MQ=255)
                  aCAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTa                                                                                                                              <  1:4613276/150‑1 (MQ=255)
                   cAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTAc                                                                                                                             <  1:3919822/150‑1 (MQ=255)
                   cAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTAc                                                                                                                             <  2:3976238/150‑1 (MQ=255)
                          aaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAtt                                                                                                                      >  1:490411/1‑150 (MQ=255)
                          aaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAtt                                                                                                                      >  1:3590528/1‑150 (MQ=255)
                           aaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTg                                                                                                                     <  2:2577439/150‑1 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                   <  1:4224499/150‑1 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                   >  1:2315108/1‑150 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                   >  1:252023/1‑150 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAAAGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                   >  1:2172399/1‑150 (MQ=255)
                                     tttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGt                                                                                                           <  1:2917892/150‑1 (MQ=255)
                                      ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                          <  2:4538453/150‑1 (MQ=255)
                                      ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                          >  1:1367152/1‑150 (MQ=255)
                                      ttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTc                                                                                                          <  1:4376937/150‑1 (MQ=255)
                                       tAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCg                                                                                                         >  1:3449875/1‑150 (MQ=255)
                                          aCATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAAGTAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTTCAACCATTGTTCATTTGGTCGCCt                                                                                                      >  2:4232132/1‑150 (MQ=255)
                                           cATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTa                                                                                                     >  1:961291/1‑150 (MQ=255)
                                            aTAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAt                                                                                                    >  2:1556194/1‑150 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                   >  2:87570/1‑150 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                   <  1:3799182/150‑1 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                   <  2:4403293/150‑1 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                   >  2:3127289/1‑150 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAGtg                                                                                                  >  2:3912109/1‑150 (MQ=255)
                                                aaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAATAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                                <  1:3025105/150‑1 (MQ=255)
                                                  aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                              <  2:1102454/150‑1 (MQ=255)
                                                    ccTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACt                                                                                            >  1:3488714/1‑150 (MQ=255)
                                                       ttAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTa                                                                                         <  1:151183/150‑1 (MQ=255)
                                                        taataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAg                                                                                        >  2:547926/1‑150 (MQ=255)
                                                         aataaACTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                       <  2:1787920/150‑1 (MQ=255)
                                                          ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGaa                                                                                      <  2:1501341/150‑1 (MQ=255)
                                                          ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGaa                                                                                      <  1:2750229/150‑1 (MQ=255)
                                                          ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGaa                                                                                      <  1:3198965/150‑1 (MQ=255)
                                                          ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGaa                                                                                      <  2:2670679/150‑1 (MQ=255)
                                                          ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGaa                                                                                      <  1:3198960/150‑1 (MQ=255)
                                                           taaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACAATTCAAGACACAAATAGCAGAAACAAAGCTAACATATAAATGAGCAATAAAATTTGATAAACAAATTTACAAACATTGATAATAAGGACGCCTATTGAGAACTTTAGAAg                                                                                     >  1:713441/1‑150 (MQ=255)
                                                            aaaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATTACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGc                                                                                    >  1:1078843/1‑150 (MQ=255)
                                                             aaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCt                                                                                   >  2:3469511/1‑150 (MQ=255)
                                                              aaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCtt                                                                                  <  1:1703741/150‑1 (MQ=255)
                                                               aTTCCATTAGTCAGACTACTTGTTTGAAGAATGACTATTCTTGACACCAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTTTTACCCAAATTTACAACCATTGTTCTTTAGGTCGCCTATTGTGCACTTTAGAAGCttt                                                                                 <  1:1345852/150‑1 (MQ=255)
                                                               aTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCttt                                                                                 >  2:3238372/1‑150 (MQ=255)
                                                               aTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCAAGAAACAAATAGGAGAAACAAATGATAGATATTAATGAGAAATTATATTTGTTACCCAAAATTACAACCAGTGTTCATTAGGTCGCCTATTTTGCACTATAGAAGCtta                                                                                 >  2:1682684/1‑149 (MQ=255)
                                                                ttCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCtttt                                                                                <  1:2866090/150‑1 (MQ=255)
                                                                 tCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTg                                                                               <  2:235794/150‑1 (MQ=255)
                                                                  ccATTAGTCAGACGACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGa                                                                              <  2:753624/150‑1 (MQ=255)
                                                                   cATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGaa                                                                             >  2:383679/1‑150 (MQ=255)
                                                                       aGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaa                                                                         >  2:3137293/1‑150 (MQ=255)
                                                                        gTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaat                                                                        <  1:4016511/150‑1 (MQ=255)
                                                                         tCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaatt                                                                       <  2:4381880/150‑1 (MQ=255)
                                                                         tCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaatt                                                                       >  1:2515913/1‑150 (MQ=255)
                                                                         tCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaatt                                                                       >  1:932528/1‑150 (MQ=255)
                                                                           aGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaattaa                                                                     >  1:1988886/1‑150 (MQ=255)
                                                                               tACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAAttt                                                                 >  1:936349/1‑150 (MQ=255)
                                                                                aCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTa                                                                <  2:2185778/150‑1 (MQ=255)
                                                                                 cATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAc                                                               <  1:3839548/150‑1 (MQ=255)
                                                                                  aTGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACt                                                              >  1:1957424/1‑150 (MQ=255)
                                                                                       tGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAtt                                                         <  2:3713968/150‑1 (MQ=255)
                                                                                        gaagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTc                                                        <  1:33857/150‑1 (MQ=255)
                                                                                        gaagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTc                                                        <  2:2441720/150‑1 (MQ=255)
                                                                                        gaagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTc                                                        <  1:4553372/150‑1 (MQ=255)
                                                                                         aagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCa                                                       >  1:12560/1‑150 (MQ=255)
                                                                                            aaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCaaaa                                                    <  1:3932196/150‑1 (MQ=255)
                                                                                                aCTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTaa                                                <  2:2967590/150‑1 (MQ=255)
                                                                                                   aTTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTGTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTa                                             >  2:3063705/1‑150 (MQ=255)
                                                                                                    ttCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaa                                            >  2:1119889/1‑150 (MQ=255)
                                                                                                      cATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaaa                                          <  1:1548217/150‑1 (MQ=255)
                                                                                                        tGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAAt                                        >  1:1720584/1‑150 (MQ=255)
                                                                                                             caAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                   <  1:4505656/150‑1 (MQ=255)
                                                                                                                aTAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcac                                >  2:3258816/1‑150 (MQ=255)
                                                                                                                   ggAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAg                             >  1:3026128/1‑150 (MQ=255)
                                                                                                                    gagaAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGt                            <  2:3548225/150‑1 (MQ=255)
                                                                                                                     agaAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTg                           <  2:3336467/150‑1 (MQ=255)
                                                                                                                     agaAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTg                           <  2:1345558/150‑1 (MQ=255)
                                                                                                                        aaCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCaa                        <  1:618078/150‑1 (MQ=255)
                                                                                                                         aCAAATGTTAGATAATAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                       <  2:3590095/150‑1 (MQ=255)
                                                                                                                           aaaTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTg                     >  2:2007760/1‑150 (MQ=255)
                                                                                                                               gTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTa                 >  1:2405478/1‑150 (MQ=255)
                                                                                                                                  aGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAggg              >  2:973520/1‑150 (MQ=255)
                                                                                                                                    atatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGATGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTGACAAGTGCGATTGGTTAGGGTa            >  2:4077165/1‑150 (MQ=255)
                                                                                                                                     tatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGAACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt           >  1:912344/1‑150 (MQ=255)
                                                                                                                                        tAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGga        >  1:1154676/1‑150 (MQ=255)
                                                                                                                                          aTGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGgaga      <  1:1038945/150‑1 (MQ=255)
                                                                                                                                           tGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGAGCAATTGGTTAGGGTATGGAGAt     >  1:2609278/1‑150 (MQ=255)
                                                                                                                                            gAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATg    <  1:3366787/150‑1 (MQ=255)
                                                                                                                                              gCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATggg  <  2:1407127/150‑1 (MQ=255)
                                                                                                                                              gCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATggg  >  2:86645/1‑150 (MQ=255)
                                                                                                                                              gCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTGAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATggg  <  2:4473420/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGG  >  CP009273/153325‑153616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGG  >  CP009273/153323‑153616
                                                                                                                                                     |                                                                                                                                                
CATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCGTGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATT                                                                                                                                                  >  SRR19973073.2432382/1‑150 (MQ=60)
CATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATT                                                                                                                                                  >  SRR19973073.253532/1‑150 (MQ=60)
  TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTAT                                                                                                                                                <  SRR19973073.2569078/150‑1 (MQ=60)
  TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTAT                                                                                                                                                <  SRR19973073.2569128/150‑1 (MQ=60)
   CGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATA                                                                                                                                               <  SRR19973073.823920/150‑1 (MQ=60)
   CGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATA                                                                                                                                               <  SRR19973073.823906/150‑1 (MQ=60)
   CGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATA                                                                                                                                               <  SRR19973073.4088810/150‑1 (MQ=60)
    GCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAT                                                                                                                                              >  SRR19973073.4435802/1‑150 (MQ=60)
      CAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTT                                                                                                                                            >  SRR19973073.3554893/1‑150 (MQ=60)
      CAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTT                                                                                                                                            >  SRR19973073.3555335/1‑150 (MQ=60)
      CAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTT                                                                                                                                            <  SRR19973073.4098419/150‑1 (MQ=60)
       AGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                           >  SRR19973073.2272893/1‑150 (MQ=60)
         CATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTT                                                                                                                                         >  SRR19973073.2936315/1‑150 (MQ=60)
         CATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTT                                                                                                                                         >  SRR19973073.2936333/1‑150 (MQ=60)
           TAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTAC                                                                                                                                       >  SRR19973073.1789828/1‑150 (MQ=60)
                  AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                >  SRR19973073.47463/1‑150 (MQ=60)
                  AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                >  SRR19973073.4018006/1‑150 (MQ=60)
                  AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATT                                                                                                                                >  SRR19973073.1688938/1‑150 (MQ=60)
                    ACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTA                                                                                                                              <  SRR19973073.4622483/150‑1 (MQ=60)
                     CAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTAC                                                                                                                             <  SRR19973073.3927620/150‑1 (MQ=60)
                     CAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTAC                                                                                                                             >  SRR19973073.1988655/1‑150 (MQ=60)
                      AGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACA                                                                                                                            <  SRR19973073.3693700/150‑1 (MQ=60)
                         GCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACC                                                                                                                         <  SRR19973073.399865/150‑1 (MQ=60)
                            AAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATT                                                                                                                      >  SRR19973073.491455/1‑150 (MQ=60)
                            AAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATT                                                                                                                      >  SRR19973073.3597727/1‑150 (MQ=60)
                              ACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGT                                                                                                                    >  SRR19973073.689239/1‑150 (MQ=60)
                              ACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGT                                                                                                                    >  SRR19973073.3417553/1‑150 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   >  SRR19973073.908830/1‑150 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   >  SRR19973073.252579/1‑150 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   >  SRR19973073.252591/1‑150 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   <  SRR19973073.4232786/150‑1 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   >  SRR19973073.908895/1‑150 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   <  SRR19973073.4232874/150‑1 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   >  SRR19973073.2319822/1‑150 (MQ=60)
                               CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAAAGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                   >  SRR19973073.2176936/1‑150 (MQ=60)
                                       TTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGT                                                                                                           <  SRR19973073.2923860/150‑1 (MQ=60)
                                        TTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTC                                                                                                          >  SRR19973073.1370054/1‑150 (MQ=60)
                                        TTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTC                                                                                                          <  SRR19973073.4385717/150‑1 (MQ=60)
                                         TAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCG                                                                                                         >  SRR19973073.3456562/1‑150 (MQ=60)
                                         TAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCG                                                                                                         >  SRR19973073.3456747/1‑150 (MQ=60)
                                         TAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCG                                                                                                         >  SRR19973073.3456791/1‑150 (MQ=60)
                                             CATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTA                                                                                                     >  SRR19973073.963303/1‑150 (MQ=60)
                                              ATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAT                                                                                                    <  SRR19973073.1366600/150‑1 (MQ=60)
                                               TAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATT                                                                                                   >  SRR19973073.3373172/1‑150 (MQ=60)
                                               TAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATT                                                                                                   <  SRR19973073.3806772/150‑1 (MQ=60)
                                                AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                  <  SRR19973073.1048348/150‑1 (MQ=60)
                                                  AAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAATAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTG                                                                                                <  SRR19973073.3031228/150‑1 (MQ=60)
                                                  AAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTG                                                                                                <  SRR19973073.3031351/150‑1 (MQ=60)
                                                     TCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCAC                                                                                             <  SRR19973073.2895471/150‑1 (MQ=60)
                                                      CCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACT                                                                                            >  SRR19973073.3495665/1‑150 (MQ=60)
                                                         TTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTA                                                                                         <  SRR19973073.4252363/150‑1 (MQ=60)
                                                         TTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTA                                                                                         >  SRR19973073.3381726/1‑150 (MQ=60)
                                                         TTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTA                                                                                         <  SRR19973073.151545/150‑1 (MQ=60)
                                                          TAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAGTGTTCATTAGGTCGCCTATTGTGCACTTTAG                                                                                        >  SRR19973073.2405269/1‑150 (MQ=60)
                                                          TAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAG                                                                                        <  SRR19973073.1528507/150‑1 (MQ=60)
                                                           AATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGA                                                                                       <  SRR19973073.286431/150‑1 (MQ=60)
                                                            ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                      >  SRR19973073.3736587/1‑150 (MQ=60)
                                                            ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                      <  SRR19973073.3205424/150‑1 (MQ=60)
                                                            ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                      <  SRR19973073.3205423/150‑1 (MQ=60)
                                                            ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                      <  SRR19973073.3205419/150‑1 (MQ=60)
                                                            ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                      <  SRR19973073.2755862/150‑1 (MQ=60)
                                                             TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                     >  SRR19973073.147786/1‑150 (MQ=60)
                                                             TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                     >  SRR19973073.3946023/1‑150 (MQ=60)
                                                             TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACAATTCAAGACACAAATAGCAGAAACAAAGCTAACATATAAATGAGCAATAAAATTTGATAAACAAATTTACAAACATTGATAATAAGGACGCCTATTGAGAACTTTAGAAG                                                                                     >  SRR19973073.714911/1‑150 (MQ=41)
                                                              AAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATTACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGC                                                                                    >  SRR19973073.1081068/1‑150 (MQ=60)
                                                              AAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGC                                                                                    <  SRR19973073.95813/150‑1 (MQ=60)
                                                                AATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTT                                                                                  <  SRR19973073.1707277/150‑1 (MQ=60)
                                                                 ATTCCATTAGTCAGACTACTTGTTTGAAGAATGACTATTCTTGACACCAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTTTTACCCAAATTTACAACCATTGTTCTTTAGGTCGCCTATTGTGCACTTTAGAAGCTTT                                                                                 <  SRR19973073.1348713/150‑1 (MQ=60)
                                                                  TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                <  SRR19973073.3884378/150‑1 (MQ=60)
                                                                  TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                <  SRR19973073.2871952/150‑1 (MQ=60)
                                                                   TCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTCGGTCGCCTATTGTGCACTTTAGAAGCTTTTG                                                                               <  SRR19973073.269235/150‑1 (MQ=60)
                                                                   TCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTG                                                                               <  SRR19973073.191428/150‑1 (MQ=60)
                                                                      ATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAC                                                                            >  SRR19973073.2241903/1‑150 (MQ=60)
                                                                       TTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACA                                                                           <  SRR19973073.1333623/150‑1 (MQ=60)
                                                                          GTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAAT                                                                        <  SRR19973073.4023798/150‑1 (MQ=60)
                                                                          GTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAAT                                                                        <  SRR19973073.4024538/150‑1 (MQ=60)
                                                                           TCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATT                                                                       >  SRR19973073.934473/1‑150 (MQ=60)
                                                                           TCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATT                                                                       >  SRR19973073.934471/1‑150 (MQ=60)
                                                                           TCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATT                                                                       >  SRR19973073.2521117/1‑150 (MQ=60)
                                                                           TCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATT                                                                       >  SRR19973073.2446111/1‑150 (MQ=60)
                                                                            CAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTA                                                                      >  SRR19973073.1578592/1‑150 (MQ=60)
                                                                             AGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAA                                                                     >  SRR19973073.1993006/1‑150 (MQ=60)
                                                                                 TACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTT                                                                 >  SRR19973073.938298/1‑150 (MQ=60)
                                                                                 TACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTT                                                                 >  SRR19973073.938317/1‑150 (MQ=60)
                                                                                   CATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAC                                                               <  SRR19973073.3847096/150‑1 (MQ=60)
                                                                                   CATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAC                                                               <  SRR19973073.3847172/150‑1 (MQ=60)
                                                                                    ATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACT                                                              >  SRR19973073.1961457/1‑150 (MQ=60)
                                                                                     TGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTT                                                             >  SRR19973073.3539681/1‑150 (MQ=60)
                                                                                         TGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATT                                                         >  SRR19973073.4557924/1‑150 (MQ=60)
                                                                                          TAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGATCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGACTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTC                                                        <  SRR19973073.4562722/150‑1 (MQ=60)
                                                                                          GAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTC                                                        <  SRR19973073.4562495/150‑1 (MQ=60)
                                                                                          GAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTC                                                        <  SRR19973073.33957/150‑1 (MQ=60)
                                                                                           AAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCA                                                       >  SRR19973073.12591/1‑150 (MQ=60)
                                                                                           AAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCA                                                       >  SRR19973073.12599/1‑150 (MQ=60)
                                                                                              AATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAA                                                    >  SRR19973073.1150849/1‑150 (MQ=60)
                                                                                              AATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAA                                                    <  SRR19973073.3940025/150‑1 (MQ=60)
                                                                                              AATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAA                                                    <  SRR19973073.3940020/150‑1 (MQ=60)
                                                                                                TGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATT                                                  <  SRR19973073.4242485/150‑1 (MQ=60)
                                                                                                       TCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAA                                           <  SRR19973073.4638468/150‑1 (MQ=60)
                                                                                                       TCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAA                                           >  SRR19973073.3565665/1‑150 (MQ=60)
                                                                                                        CATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAA                                          <  SRR19973073.1551383/150‑1 (MQ=60)
                                                                                                        CATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAA                                          >  SRR19973073.3265218/1‑150 (MQ=60)
                                                                                                          TGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAAT                                        >  SRR19973073.1724159/1‑150 (MQ=60)
                                                                                                               CAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTT                                   <  SRR19973073.4514709/150‑1 (MQ=60)
                                                                                                                 AATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCA                                 >  SRR19973073.3868771/1‑150 (MQ=60)
                                                                                                                 AATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCA                                 >  SRR19973073.2869993/1‑150 (MQ=60)
                                                                                                                   TAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACA                               <  SRR19973073.937282/150‑1 (MQ=60)
                                                                                                                     GGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAG                             >  SRR19973073.3032286/1‑150 (MQ=60)
                                                                                                                     GGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAG                             >  SRR19973073.3032252/1‑150 (MQ=60)
                                                                                                                          AACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAA                        <  SRR19973073.592851/150‑1 (MQ=60)
                                                                                                                          AACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAA                        <  SRR19973073.619401/150‑1 (MQ=60)
                                                                                                                              AATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGG                    <  SRR19973073.2100645/150‑1 (MQ=60)
                                                                                                                                 GTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTA                 <  SRR19973073.452645/150‑1 (MQ=60)
                                                                                                                                 GTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTA                 >  SRR19973073.2410423/1‑150 (MQ=60)
                                                                                                                                  TTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAG                <  SRR19973073.2023801/150‑1 (MQ=60)
                                                                                                                                      ATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTA            <  SRR19973073.1480808/150‑1 (MQ=60)
                                                                                                                                       TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT           <  SRR19973073.2317927/150‑1 (MQ=60)
                                                                                                                                       TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT           <  SRR19973073.2317806/150‑1 (MQ=60)
                                                                                                                                       TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT           >  SRR19973073.914720/1‑150 (MQ=60)
                                                                                                                                       TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGAACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT           >  SRR19973073.914235/1‑150 (MQ=60)
                                                                                                                                          TAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGA        <  SRR19973073.2477592/150‑1 (MQ=60)
                                                                                                                                          TAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGA        >  SRR19973073.1157016/1‑150 (MQ=60)
                                                                                                                                          TAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGA        >  SRR19973073.1156997/1‑150 (MQ=60)
                                                                                                                                           AATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAG       >  SRR19973073.1340470/1‑150 (MQ=60)
                                                                                                                                            ATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGA      <  SRR19973073.1041114/150‑1 (MQ=60)
                                                                                                                                             TGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGAT     <  SRR19973073.3196970/150‑1 (MQ=60)
                                                                                                                                             TGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGAGCAATTGGTTAGGGTATGGAGAT     >  SRR19973073.2614650/1‑150 (MQ=60)
                                                                                                                                              GAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATG    <  SRR19973073.3373572/150‑1 (MQ=60)
                                                                                                                                               AGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGG   >  SRR19973073.629509/1‑150 (MQ=60)
                                                                                                                                               AGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGG   >  SRR19973073.1718664/1‑150 (MQ=60)
                                                                                                                                                GCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTAACAAGTGCAATTGGTTAGGGTATGGAGATGGG  >  SRR19973073.4082775/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
CATCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGG  >  CP009273/153323‑153616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑