Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 153,472 G→T 92.7% intergenic (‑102/+268) yadN ← / ← folK putative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273153,4720GT92.7% 447.1 / 24.3 150intergenic (‑102/+268)yadN/folKputative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base G (3/8);  new base T (66/73);  total (69/81)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 151 total aligned reads displayed.

TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGG  >  CP009273/153325‑153615
                                                                                                                                                   |                                                                                                                                               
tCGCCAGCATAAGACCAGACAGGGCAAAACCAAATTTTTTAGACATAAAAATCCTTAAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTat                                                                                                                                               <  2:1669005/150‑1 (MQ=255)
 cGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATtata                                                                                                                                              <  1:2033133/150‑1 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                             <  1:3806624/150‑1 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                             <  2:2470842/150‑1 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                             <  1:1841220/150‑1 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAACTCCATTAGTCAGACTTCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                             <  1:3720643/150‑1 (MQ=255)
   ccAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAtt                                                                                                                                            <  2:3147635/150‑1 (MQ=255)
   ccAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAtt                                                                                                                                            <  2:2577824/150‑1 (MQ=255)
    cAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAttt                                                                                                                                           <  2:2592569/150‑1 (MQ=255)
    cAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAttt                                                                                                                                           <  2:417785/150‑1 (MQ=255)
     aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                          <  1:824464/150‑1 (MQ=255)
     aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                          <  1:824571/150‑1 (MQ=255)
     aGCAAAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTg                                                                                                                                          <  1:1518340/150‑1 (MQ=255)
            gACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCa                                                                                                                                   <  1:840742/150‑1 (MQ=255)
             aCCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCaa                                                                                                                                  >  1:4165980/1‑150 (MQ=255)
             aCCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCaa                                                                                                                                  <  2:3527768/150‑1 (MQ=255)
                aGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAAtt                                                                                                                               <  2:3331909/150‑1 (MQ=255)
                    aGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACa                                                                                                                           >  1:2276620/1‑150 (MQ=255)
                       gCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACAGGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAAcc                                                                                                                        >  2:3965932/1‑150 (MQ=255)
                           aaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTg                                                                                                                    <  1:1740925/150‑1 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                  >  1:2877533/1‑150 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                  >  2:3620614/1‑150 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTACATTAGTCAGACTAAATGTTTGAAGAATGACTATTCATTACACAAATAGGAGAAACAAATGTTAGATATTAATGTGCAATTATATTTGTTAACCAAATTTACAACCAATgat                                                                                                                  >  1:3321730/1‑148 (MQ=255)
                              cTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTc                                                                                                                 <  2:2964695/150‑1 (MQ=255)
                              cTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTc                                                                                                                 >  2:1554757/1‑150 (MQ=255)
                                   tttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAg                                                                                                            <  1:75302/150‑1 (MQ=255)
                                    ttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAgg                                                                                                           >  2:807865/1‑150 (MQ=255)
                                           cATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTa                                                                                                    >  2:3842771/1‑150 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAACTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                  <  2:4323182/150‑1 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                 >  2:1477919/1‑150 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                 >  2:3668158/1‑150 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGACGCCTATtg                                                                                                 >  1:3526618/1‑150 (MQ=255)
                                                aaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgtg                                                                                               >  1:135510/1‑150 (MQ=255)
                                                 aaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGc                                                                                              <  2:3069487/150‑1 (MQ=255)
                                                 aaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGc                                                                                              <  2:3069474/150‑1 (MQ=255)
                                                  aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                             <  1:4236150/150‑1 (MQ=255)
                                                   tCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCAc                                                                                            <  1:3592140/150‑1 (MQ=255)
                                                         aataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                      >  2:4344796/1‑150 (MQ=255)
                                                         aataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGa                                                                                      <  2:3489350/150‑1 (MQ=255)
                                                          ataaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGaa                                                                                     <  1:3068606/150‑1 (MQ=255)
                                                           taaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAg                                                                                    <  1:3629252/150‑1 (MQ=255)
                                                           taaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAg                                                                                    <  2:3837208/150‑1 (MQ=255)
                                                            aaaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGc                                                                                   <  1:2833403/150‑1 (MQ=255)
                                                             aaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCt                                                                                  >  2:173846/1‑150 (MQ=255)
                                                             aaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCt                                                                                  <  2:1287907/150‑1 (MQ=255)
                                                              aaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCtt                                                                                 >  1:2722511/1‑150 (MQ=255)
                                                               aTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCttt                                                                                >  1:1902195/1‑150 (MQ=255)
                                                                    aTTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAc                                                                           >  1:477511/1‑150 (MQ=255)
                                                                    aTTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAc                                                                           >  1:479195/1‑150 (MQ=255)
                                                                    aTTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAc                                                                           >  2:351999/1‑150 (MQ=255)
                                                                         tCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaatt                                                                      <  2:4007564/150‑1 (MQ=255)
                                                                         tCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaatt                                                                      >  1:4032149/1‑150 (MQ=255)
                                                                             aCTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaattaaat                                                                  <  1:934888/150‑1 (MQ=255)
                                                                                aCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTa                                                               >  1:3200551/1‑150 (MQ=255)
                                                                                 cATGTTTGAAGAATGACTATTCATGACACAAATAGTAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAc                                                              <  1:3022261/150‑1 (MQ=255)
                                                                                   tGTTTTAAGAATGTCTATTCATGACTCAAATAGGAGAAACAAATGTTAGATATTAATGATCAATTATATTTGTTACCCAAATTTACAACCATTTTTCATTAGTTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACtt                                                            <  2:1549123/150‑1 (MQ=255)
                                                                                   tGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACtt                                                            <  1:3668158/150‑1 (MQ=255)
                                                                                   tGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACtt                                                            <  2:1551421/150‑1 (MQ=255)
                                                                                    gTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTa                                                           <  2:3768501/150‑1 (MQ=255)
                                                                                      ttGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAATTTTACTTAAt                                                         >  2:3487778/1‑150 (MQ=255)
                                                                                       tGAAGAATGACGATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAtt                                                        <  2:598838/150‑1 (MQ=255)
                                                                                        gaagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTc                                                       <  1:3040983/150‑1 (MQ=255)
                                                                                        gaagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTc                                                       >  2:3825231/1‑150 (MQ=255)
                                                                                        gaagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTc                                                       >  2:1969607/1‑150 (MQ=255)
                                                                                         aagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCa                                                      <  2:1075983/150‑1 (MQ=255)
                                                                                              tGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAAtt                                                 >  1:1559890/1‑150 (MQ=255)
                                                                                              tGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAAtt                                                 <  2:135553/150‑1 (MQ=255)
                                                                                              tGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATGAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAAtt                                                 <  1:3513497/150‑1 (MQ=255)
                                                                                                aCTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTaa                                               >  2:660905/1‑150 (MQ=255)
                                                                                                 cTATTCATGACACAAATAGGAGAAACAAATTTTAGATATTAATGAGCATTGATATTTGTTACCCAAATTTACAACAATTGTTAATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAg                                              <  2:4263786/150‑1 (MQ=255)
                                                                                                    ttCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaa                                           >  1:1106440/1‑150 (MQ=255)
                                                                                                     tCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaa                                          >  2:3147979/1‑150 (MQ=255)
                                                                                                      cATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaaa                                         <  2:1445951/150‑1 (MQ=255)
                                                                                                       aTGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaaaa                                        >  2:1875972/1‑150 (MQ=255)
                                                                                                         gACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATa                                      >  2:2296837/1‑150 (MQ=255)
                                                                                                          acacAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATaa                                     <  1:807865/150‑1 (MQ=255)
                                                                                                             caAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTTTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTTAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                  >  2:67000/1‑150 (MQ=255)
                                                                                                               aaTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTca                                >  2:3306821/1‑150 (MQ=255)
                                                                                                                ctAGGAGACACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAACTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcac                               <  2:260492/149‑1 (MQ=255)
                                                                                                                 tAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcaca                              >  1:3993271/1‑150 (MQ=255)
                                                                                                                  aGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACaa                             >  1:4140881/1‑150 (MQ=255)
                                                                                                                  aGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAATTAATTCAAAATTAATTAAAAATAATTTCaaaa                             >  2:2890541/1‑150 (MQ=255)
                                                                                                                      gaAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGc                         <  1:3174359/150‑1 (MQ=255)
                                                                                                                      gaAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGc                         <  2:2060596/150‑1 (MQ=255)
                                                                                                                        aaCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCaa                       >  1:142035/1‑150 (MQ=255)
                                                                                                                         aCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                      >  1:3722815/1‑150 (MQ=255)
                                                                                                                         aCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                      <  1:356578/150‑1 (MQ=255)
                                                                                                                         aCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                      >  1:1690577/1‑150 (MQ=255)
                                                                                                                         aCAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAt                      <  1:2528830/150‑1 (MQ=255)
                                                                                                                           aaaTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTg                    >  1:36344/1‑150 (MQ=255)
                                                                                                                            aaTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTgg                   >  2:607799/1‑150 (MQ=255)
                                                                                                                               gTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTa                <  2:1167765/150‑1 (MQ=255)
                                                                                                                                   gATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGt            <  1:1969607/150‑1 (MQ=255)
                                                                                                                                     tatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt          >  1:1472445/1‑150 (MQ=255)
                                                                                                                                     tatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt          <  1:3753620/150‑1 (MQ=255)
                                                                                                                                      atTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTATTGAACAAAATAAAATTACTTAATTCAAAATTAAGTAAAAATAAGATCACAAGTGCAATTGGGTAGGGTATg         >  1:2457787/1‑150 (MQ=255)
                                                                                                                                          aTGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGgaga     >  1:3082318/1‑150 (MQ=255)
                                                                                                                                           tGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGAt    <  1:3414960/150‑1 (MQ=255)
                                                                                                                                             aGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATgg  >  2:133251/1‑150 (MQ=255)
                                                                                                                                             aGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAGTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATgg  >  2:133343/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGG  >  CP009273/153325‑153615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  >  CP009273/153326‑153621
                                                                                                                                                  |                                                                                                                                                     
CGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATA                                                                                                                                                    <  SRR19973079.2043827/150‑1 (MQ=60)
 GCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAT                                                                                                                                                   <  SRR19973079.3826103/150‑1 (MQ=60)
 GCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAT                                                                                                                                                   <  SRR19973079.1850923/150‑1 (MQ=60)
 GCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAACTCCATTAGTCAGACTTCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAT                                                                                                                                                   <  SRR19973079.3739692/150‑1 (MQ=60)
   CAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTT                                                                                                                                                 <  SRR19973079.3565980/150‑1 (MQ=60)
    AGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                                <  SRR19973079.829396/150‑1 (MQ=60)
    AGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                                <  SRR19973079.829322/150‑1 (MQ=60)
    AGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                                <  SRR19973079.829214/150‑1 (MQ=60)
    AGCAAAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTG                                                                                                                                                <  SRR19973079.1526708/150‑1 (MQ=60)
     GCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGT                                                                                                                                               <  SRR19973079.1783123/150‑1 (MQ=60)
        TAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTAC                                                                                                                                            <  SRR19973079.75174/150‑1 (MQ=60)
           GACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCA                                                                                                                                         <  SRR19973079.845609/150‑1 (MQ=60)
            ACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAA                                                                                                                                        >  SRR19973079.2814770/1‑150 (MQ=60)
            ACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAA                                                                                                                                        >  SRR19973079.4186835/1‑150 (MQ=60)
            ACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAA                                                                                                                                        >  SRR19973079.4187113/1‑150 (MQ=60)
                   AGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACA                                                                                                                                 >  SRR19973079.2288447/1‑150 (MQ=60)
                       CAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATAACACAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCA                                                                                                                             >  SRR19973079.327781/1‑150 (MQ=60)
                          AACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTG                                                                                                                          <  SRR19973079.1750144/150‑1 (MQ=60)
                           ACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGT                                                                                                                         <  SRR19973079.1678301/150‑1 (MQ=60)
                            CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                        >  SRR19973079.2892102/1‑150 (MQ=60)
                            CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTACATTAGTCAGACTAAATGTTTGAAGAATGACTATTCATTACACAAATAGGAGAAACAAATGTTAGATATTAATGTGCAATTATATTTGTTAACCAAATTTACAACCAatgat                                                                                                                        >  SRR19973079.3338711/1‑145 (MQ=60)
                                  TTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAG                                                                                                                  <  SRR19973079.76011/150‑1 (MQ=60)
                                             AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGACGCCTATTG                                                                                                       >  SRR19973079.3544718/1‑150 (MQ=60)
                                               AAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTG                                                                                                     >  SRR19973079.136597/1‑150 (MQ=60)
                                               AAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTG                                                                                                     >  SRR19973079.136640/1‑150 (MQ=60)
                                                 ATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCA                                                                                                   <  SRR19973079.4257583/150‑1 (MQ=60)
                                                  TCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCAC                                                                                                  <  SRR19973079.3610404/150‑1 (MQ=60)
                                                         ATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAA                                                                                           <  SRR19973079.3084493/150‑1 (MQ=60)
                                                          TAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAG                                                                                          <  SRR19973079.3647602/150‑1 (MQ=60)
                                                           AAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGC                                                                                         <  SRR19973079.2847863/150‑1 (MQ=60)
                                                           AAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTAGTTACACAAATTTAAAACCATTGTTCATTAGGTCGCCTATTGTGCAatatataagc                                                                                         >  SRR19973079.1918347/1‑140 (MQ=60)
                                                             AATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTT                                                                                       >  SRR19973079.2736591/1‑150 (MQ=60)
                                                              ATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTT                                                                                      >  SRR19973079.1912325/1‑150 (MQ=60)
                                                               TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                     >  SRR19973079.816049/1‑150 (MQ=60)
                                                                 CCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTATATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGATCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGA                                                                                   >  SRR19973079.3676010/1‑150 (MQ=60)
                                                                   ATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAC                                                                                 >  SRR19973079.482097/1‑150 (MQ=60)
                                                                   ATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAC                                                                                 >  SRR19973079.480404/1‑150 (MQ=60)
                                                                        TCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATT                                                                            >  SRR19973079.4052507/1‑150 (MQ=60)
                                                                            ACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAAT                                                                        <  SRR19973079.940266/150‑1 (MQ=60)
                                                                               ACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTA                                                                     >  SRR19973079.3216879/1‑150 (MQ=60)
                                                                                CATGTTTGAAGAATGACTATTCATGACACAAATAGTAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAC                                                                    <  SRR19973079.3037888/150‑1 (MQ=60)
                                                                                  TGTTTGAAGAATGACTATTGATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTT                                                                  <  SRR19973079.1485991/150‑1 (MQ=60)
                                                                                  TGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTT                                                                  <  SRR19973079.3686845/150‑1 (MQ=60)
                                                                                    TTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAA                                                                <  SRR19973079.2979459/150‑1 (MQ=60)
                                                                                       GAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTC                                                             <  SRR19973079.3056732/150‑1 (MQ=60)
                                                                                       GAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTC                                                             <  SRR19973079.3795968/150‑1 (MQ=60)
                                                                                             TGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATT                                                       <  SRR19973079.3531483/150‑1 (MQ=60)
                                                                                             TGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATT                                                       >  SRR19973079.1568501/1‑150 (MQ=60)
                                                                                             TGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATGAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATT                                                       <  SRR19973079.3531565/150‑1 (MQ=60)
                                                                                                   TTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAA                                                 >  SRR19973079.1112657/1‑150 (MQ=60)
                                                                                                         ACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAA                                           <  SRR19973079.812486/150‑1 (MQ=60)
                                                                                                                TAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACA                                    >  SRR19973079.4013306/1‑150 (MQ=60)
                                                                                                                 AGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAA                                   >  SRR19973079.4161895/1‑150 (MQ=60)
                                                                                                                    AGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTG                                <  SRR19973079.30833/150‑1 (MQ=60)
                                                                                                                     GAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGC                               <  SRR19973079.3190610/150‑1 (MQ=60)
                                                                                                                       AACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAA                             >  SRR19973079.143159/1‑150 (MQ=60)
                                                                                                                        ACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAT                            <  SRR19973079.2541587/150‑1 (MQ=60)
                                                                                                                        ACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAT                            >  SRR19973079.3741854/1‑150 (MQ=60)
                                                                                                                        ACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAT                            >  SRR19973079.3741877/1‑150 (MQ=60)
                                                                                                                        ACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAT                            >  SRR19973079.1699664/1‑150 (MQ=60)
                                                                                                                        ACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAAT                            <  SRR19973079.358504/150‑1 (MQ=60)
                                                                                                                          AAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTG                          <  SRR19973079.4337966/150‑1 (MQ=60)
                                                                                                                          AAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTG                          >  SRR19973079.36745/1‑150 (MQ=60)
                                                                                                                            ATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGT                        >  SRR19973079.2273722/1‑150 (MQ=60)
                                                                                                                                  GATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGT                  <  SRR19973079.1980081/150‑1 (MQ=60)
                                                                                                                                    TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT                >  SRR19973079.1480480/1‑150 (MQ=60)
                                                                                                                                    TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT                <  SRR19973079.3772850/150‑1 (MQ=60)
                                                                                                                                     ATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATG               >  SRR19973079.2470463/1‑150 (MQ=60)
                                                                                                                                     ATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATG               <  SRR19973079.3638934/150‑1 (MQ=60)
                                                                                                                                     ATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTATTGAACAAAATAAAATTACTTAATTCAAAATTAAGTAAAAATAAGATCACAAGTGCAATTGGGTAGGGTATG               >  SRR19973079.2470383/1‑150 (MQ=60)
                                                                                                                                       TAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGA             >  SRR19973079.4021503/1‑150 (MQ=60)
                                                                                                                                         ATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGA           >  SRR19973079.3098274/1‑150 (MQ=60)
                                                                                                                                         ATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGA           >  SRR19973079.3098260/1‑150 (MQ=60)
                                                                                                                                          TGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGAT          <  SRR19973079.3432590/150‑1 (MQ=60)
                                                                                                                                           GAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATG         <  SRR19973079.952371/150‑1 (MQ=60)
                                                                                                                                                ATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATT    <  SRR19973079.257856/150‑1 (MQ=60)
                                                                                                                                                  TATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  <  SRR19973079.3447995/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  >  CP009273/153326‑153621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑