Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 153,472 G→T 93.2% intergenic (‑102/+268) yadN ← / ← folK putative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273153,4720GT93.2% 843.6 / 48.8 273intergenic (‑102/+268)yadN/folKputative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base G (7/12);  new base T (119/135);  total (126/147)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 274 total aligned reads displayed.

TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  CP009273/153325‑153618
                                                                                                                                                   |                                                                                                                                                  
tCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTat                                                                                                                                                  <  2:55994/150‑1 (MQ=255)
tCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTat                                                                                                                                                  <  1:3426017/150‑1 (MQ=255)
  gCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTatat                                                                                                                                                <  2:3215497/150‑1 (MQ=255)
   ccAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATAtt                                                                                                                                               <  1:886026/150‑1 (MQ=255)
     aGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTg                                                                                                                                             >  2:2451192/1‑150 (MQ=255)
       cATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGtt                                                                                                                                           <  2:3053899/150‑1 (MQ=255)
       cATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGtt                                                                                                                                           <  1:4682834/150‑1 (MQ=255)
         tAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTAc                                                                                                                                         <  1:1124889/150‑1 (MQ=255)
          aaGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTAcc                                                                                                                                        <  2:5112386/150‑1 (MQ=255)
           aGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTAccc                                                                                                                                       >  2:1052089/1‑150 (MQ=255)
            gACCACACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCa                                                                                                                                      <  2:3784325/150‑1 (MQ=255)
             aCCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCaa                                                                                                                                     >  2:3496090/1‑150 (MQ=255)
             aCCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCaa                                                                                                                                     >  2:3268895/1‑150 (MQ=255)
               cAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAAt                                                                                                                                   >  2:4499872/1‑150 (MQ=255)
                  aCAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTa                                                                                                                                >  2:3279744/1‑150 (MQ=255)
                        cAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCa                                                                                                                          >  1:2695931/1‑150 (MQ=255)
                         aaaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAt                                                                                                                         >  1:2255497/1‑150 (MQ=255)
                          aaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAtt                                                                                                                        >  2:2662248/1‑150 (MQ=255)
                          aaaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAtt                                                                                                                        >  1:1642422/1‑150 (MQ=255)
                           aaCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTg                                                                                                                       <  1:3483546/150‑1 (MQ=255)
                            aCCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGt                                                                                                                      >  1:179028/1‑150 (MQ=255)
                             ccTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGtt                                                                                                                     <  1:332378/150‑1 (MQ=255)
                              cTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTc                                                                                                                    >  2:1840861/1‑150 (MQ=255)
                                aaTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCAt                                                                                                                  >  1:4538307/1‑150 (MQ=255)
                                aaTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCAt                                                                                                                  >  1:2720080/1‑150 (MQ=255)
                                 aTTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCAtt                                                                                                                 >  2:3857308/1‑150 (MQ=255)
                                   tttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAg                                                                                                               <  1:54285/150‑1 (MQ=255)
                                   tttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAg                                                                                                               <  2:3358614/150‑1 (MQ=255)
                                    ttttAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAGTGTTCATTAgg                                                                                                              >  1:2126160/1‑150 (MQ=255)
                                         gACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGcc                                                                                                         <  2:4454942/150‑1 (MQ=255)
                                         gACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGcc                                                                                                         <  1:3170469/150‑1 (MQ=255)
                                         gACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGcc                                                                                                         >  1:610431/1‑150 (MQ=255)
                                           cATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTa                                                                                                       >  2:2738220/1‑150 (MQ=255)
                                            aTAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAt                                                                                                      <  2:4000283/150‑1 (MQ=255)
                                            aTAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAt                                                                                                      >  1:3582018/1‑150 (MQ=255)
                                            aTAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAt                                                                                                      <  1:2839949/150‑1 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                     <  1:5147418/150‑1 (MQ=255)
                                             tAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAtt                                                                                                     <  2:2021717/150‑1 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    >  1:2188127/1‑150 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    >  1:4370254/1‑150 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    <  1:4022486/150‑1 (MQ=255)
                                              aaaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtg                                                                                                    >  1:3874842/1‑150 (MQ=255)
                                               aaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgt                                                                                                   >  2:787149/1‑150 (MQ=255)
                                               aaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgt                                                                                                   <  1:4758734/150‑1 (MQ=255)
                                               aaaaTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATtgt                                                                                                   <  1:3287663/150‑1 (MQ=255)
                                                  aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                                <  2:2225674/150‑1 (MQ=255)
                                                  aTCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCa                                                                                                <  2:1661743/150‑1 (MQ=255)
                                                      tttAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACttt                                                                                            <  2:3621272/150‑1 (MQ=255)
                                                       ttAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTa                                                                                           >  2:3764143/1‑150 (MQ=255)
                                                           taaAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAg                                                                                       >  2:1000113/1‑150 (MQ=255)
                                                            aaaaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGc                                                                                      <  2:670737/150‑1 (MQ=255)
                                                              aaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCtt                                                                                    >  2:1582426/1‑150 (MQ=255)
                                                              aaTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCtt                                                                                    >  1:4113523/1‑150 (MQ=255)
                                                               aTTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCttt                                                                                   <  1:1011208/150‑1 (MQ=255)
                                                                  ccATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGa                                                                                >  1:2783408/1‑150 (MQ=255)
                                                                  ccATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGa                                                                                >  2:4850655/1‑150 (MQ=255)
                                                                   cATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGaa                                                                               <  1:1887802/150‑1 (MQ=255)
                                                                   cATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTGTAGAAGCTTTTGaa                                                                               >  1:3558931/1‑150 (MQ=255)
                                                                    aTTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAc                                                                              <  2:1212608/150‑1 (MQ=255)
                                                                     ttAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACa                                                                             <  1:698218/150‑1 (MQ=255)
                                                                      tAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaa                                                                            >  1:3158857/1‑150 (MQ=255)
                                                                      tAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaa                                                                            <  2:3778050/150‑1 (MQ=255)
                                                                      tAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaa                                                                            <  2:315383/150‑1 (MQ=255)
                                                                       actCAGACAACATGACCGAAGAATGACTATTCAGGACACCACTAGGAGCAACAACTGTTAGATATTACTGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaa                                                                           <  2:3697464/148‑1 (MQ=255)
                                                                        gTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaat                                                                          >  2:4297925/1‑150 (MQ=255)
                                                                        gTCAGACGACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaat                                                                          <  1:4508806/150‑1 (MQ=255)
                                                                           aGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaattaa                                                                       <  2:3983291/150‑1 (MQ=255)
                                                                             aCTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACaaattaaat                                                                     >  2:309188/1‑150 (MQ=255)
                                                                                aCATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTa                                                                  >  1:2079639/1‑150 (MQ=255)
                                                                                     tttGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTaa                                                             <  2:4164321/150‑1 (MQ=255)
                                                                                         aagaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCa                                                         >  2:4739859/1‑150 (MQ=255)
                                                                                          agaaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCaa                                                        <  2:2255497/150‑1 (MQ=255)
                                                                                            aaTGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCaaaa                                                      <  1:3077316/150‑1 (MQ=255)
                                                                                                aCTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTaa                                                  <  2:138966/150‑1 (MQ=255)
                                                                                                     tCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTaaa                                             >  1:434005/1‑150 (MQ=255)
                                                                                                        tGACACATATAGGAGAAACAAATGTTAGATATTTATGAGCAATTATATTTTTTACCCAAATTTACAACCATTGTTCATTAGGTCTCCTATTGTGCACTTTAGAAGCTTTTGAAAAAATTAAATTTACTTAATTCAAAATTAAGTAAAAAt                                          <  2:4788927/150‑1 (MQ=255)
                                                                                                         gACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATa                                         >  1:5045364/1‑150 (MQ=255)
                                                                                                           cacaAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAg                                       >  2:213180/1‑150 (MQ=255)
                                                                                                            acaAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGt                                      <  1:1187118/150‑1 (MQ=255)
                                                                                                             caAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                     >  2:2500865/1‑150 (MQ=255)
                                                                                                             caAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGtt                                     <  1:4851089/150‑1 (MQ=255)
                                                                                                                aTAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcac                                  <  2:4810469/150‑1 (MQ=255)
                                                                                                                 tAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcaca                                 <  2:1693786/150‑1 (MQ=255)
                                                                                                                 tAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTcaca                                 >  1:4924521/1‑150 (MQ=255)
                                                                                                                  aGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACaa                                <  1:787149/150‑1 (MQ=255)
                                                                                                                  aGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACaa                                <  1:3863436/150‑1 (MQ=255)
                                                                                                                   ggAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAg                               >  1:1434038/1‑150 (MQ=255)
                                                                                                                           aaaTGTAAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTg                       >  1:1543425/1‑150 (MQ=255)
                                                                                                                            aaTGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTgg                      >  1:4666765/1‑150 (MQ=255)
                                                                                                                             aTGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGt                     >  1:3648681/1‑150 (MQ=255)
                                                                                                                                ttAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAg                  >  1:2203598/1‑150 (MQ=255)
                                                                                                                                    atatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTa              >  1:2014201/1‑150 (MQ=255)
                                                                                                                                     tatTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAt             >  1:3242029/1‑150 (MQ=255)
                                                                                                                                      atTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATg            >  1:4263547/1‑150 (MQ=255)
                                                                                                                                      atTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATg            <  2:4213687/150‑1 (MQ=255)
                                                                                                                                      atTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATg            <  2:1651715/150‑1 (MQ=255)
                                                                                                                                       ttAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATgg           <  2:159325/150‑1 (MQ=255)
                                                                                                                                              gCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATggg    >  1:3282000/1‑150 (MQ=255)
                                                                                                                                               cAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGa   <  2:4370254/150‑1 (MQ=255)
                                                                                                                                                aaTTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAt  <  2:4560903/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAT  >  CP009273/153325‑153618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  >  CP009273/153325‑153621
                                                                                                                                                   |                                                                                                                                                     
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTAT                                                                                                                                                     <  SRR19973077.721254/150‑1 (MQ=60)
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTAT                                                                                                                                                     <  SRR19973077.3432031/150‑1 (MQ=60)
 CGCCAGCATAAGACCAGACAGGTCAAAACCTAATTTTTTAGACATAAAAATACTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAATAATGTCTATTCATTACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATA                                                                                                                                                    <  SRR19973077.3478009/150‑1 (MQ=60)
   CCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATT                                                                                                                                                  <  SRR19973077.887587/150‑1 (MQ=60)
    CAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTT                                                                                                                                                 >  SRR19973077.2084844/1‑150 (MQ=60)
       CATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTT                                                                                                                                              <  SRR19973077.4691096/150‑1 (MQ=60)
       CATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTT                                                                                                                                              <  SRR19973077.4691072/150‑1 (MQ=60)
       CATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTT                                                                                                                                              <  SRR19973077.4691044/150‑1 (MQ=60)
       CATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTT                                                                                                                                              <  SRR19973077.4690933/150‑1 (MQ=60)
        ATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTA                                                                                                                                             <  SRR19973077.4086271/150‑1 (MQ=60)
         TAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTAC                                                                                                                                            <  SRR19973077.1126850/150‑1 (MQ=60)
          AAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACC                                                                                                                                           <  SRR19973077.3762739/150‑1 (MQ=60)
          AAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACC                                                                                                                                           <  SRR19973077.448088/150‑1 (MQ=60)
             ACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACACATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAA                                                                                                                                        <  SRR19973077.3171512/150‑1 (MQ=60)
               CAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAAT                                                                                                                                      <  SRR19973077.1769885/150‑1 (MQ=60)
                AGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATT                                                                                                                                     >  SRR19973077.2025235/1‑150 (MQ=60)
                 GACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTT                                                                                                                                    >  SRR19973077.739089/1‑150 (MQ=60)
                   CAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTAC                                                                                                                                  <  SRR19973077.4282124/150‑1 (MQ=60)
                        CAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCA                                                                                                                             >  SRR19973077.2700734/1‑150 (MQ=60)
                         AAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAT                                                                                                                            >  SRR19973077.2259458/1‑150 (MQ=60)
                          AAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATT                                                                                                                           <  SRR19973077.190215/150‑1 (MQ=60)
                          AAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATT                                                                                                                           >  SRR19973077.1645265/1‑150 (MQ=60)
                           AACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTG                                                                                                                          <  SRR19973077.3489715/150‑1 (MQ=60)
                           AACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTG                                                                                                                          <  SRR19973077.3489718/150‑1 (MQ=60)
                            ACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGT                                                                                                                         >  SRR19973077.179370/1‑150 (MQ=60)
                            ACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGT                                                                                                                         >  SRR19973077.179296/1‑150 (MQ=60)
                             CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                        <  SRR19973077.332967/150‑1 (MQ=60)
                             CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                        <  SRR19973077.3608992/150‑1 (MQ=60)
                             CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                        <  SRR19973077.3574184/150‑1 (MQ=60)
                             CCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTT                                                                                                                        <  SRR19973077.3496974/150‑1 (MQ=60)
                               TAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCA                                                                                                                      >  SRR19973077.2257897/1‑150 (MQ=60)
                                AATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCAT                                                                                                                     >  SRR19973077.4546299/1‑150 (MQ=60)
                                AATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCAT                                                                                                                     >  SRR19973077.4546324/1‑150 (MQ=60)
                                AATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCAT                                                                                                                     >  SRR19973077.2724930/1‑150 (MQ=60)
                                   TTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAG                                                                                                                  <  SRR19973077.54406/150‑1 (MQ=60)
                                   TTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAG                                                                                                                  >  SRR19973077.4545604/1‑150 (MQ=60)
                                   TTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAG                                                                                                                  >  SRR19973077.1821893/1‑150 (MQ=60)
                                    TTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCAGTGTTCATTAGG                                                                                                                 >  SRR19973077.2129838/1‑150 (MQ=60)
                                     TTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGT                                                                                                                >  SRR19973077.5042389/1‑150 (MQ=60)
                                     TTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGT                                                                                                                >  SRR19973077.5042448/1‑150 (MQ=60)
                                         GACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCC                                                                                                            <  SRR19973077.3176088/150‑1 (MQ=60)
                                         GACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCC                                                                                                            >  SRR19973077.611524/1‑150 (MQ=60)
                                            ATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAT                                                                                                         >  SRR19973077.3588362/1‑150 (MQ=60)
                                            ATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTAT                                                                                                         <  SRR19973077.2844997/150‑1 (MQ=60)
                                             TAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATT                                                                                                        <  SRR19973077.5156533/150‑1 (MQ=60)
                                              AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                       >  SRR19973077.2191309/1‑150 (MQ=60)
                                              AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                       >  SRR19973077.2191920/1‑150 (MQ=60)
                                              AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                       >  SRR19973077.4377980/1‑150 (MQ=60)
                                              AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                       <  SRR19973077.4029603/150‑1 (MQ=60)
                                              AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                       >  SRR19973077.3881648/1‑150 (MQ=60)
                                              AAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTG                                                                                                       >  SRR19973077.3774513/1‑150 (MQ=60)
                                               AAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGT                                                                                                      <  SRR19973077.2147009/150‑1 (MQ=60)
                                               AAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGT                                                                                                      <  SRR19973077.4767149/150‑1 (MQ=60)
                                               AAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGT                                                                                                      <  SRR19973077.3293479/150‑1 (MQ=60)
                                               AAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGT                                                                                                      <  SRR19973077.3293475/150‑1 (MQ=60)
                                                     CTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTT                                                                                                >  SRR19973077.1377153/1‑150 (MQ=60)
                                                              AATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTT                                                                                       >  SRR19973077.4120776/1‑150 (MQ=60)
                                                               ATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTT                                                                                      <  SRR19973077.1012997/150‑1 (MQ=60)
                                                               ATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTT                                                                                      <  SRR19973077.2780923/150‑1 (MQ=60)
                                                                TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                     <  SRR19973077.4228593/150‑1 (MQ=60)
                                                                TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                     <  SRR19973077.4228416/150‑1 (MQ=60)
                                                                TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                     >  SRR19973077.83413/1‑150 (MQ=60)
                                                                TTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTT                                                                                     <  SRR19973077.474548/150‑1 (MQ=60)
                                                                 TCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTG                                                                                    <  SRR19973077.4800717/150‑1 (MQ=60)
                                                                 TCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTG                                                                                    >  SRR19973077.2937837/1‑150 (MQ=60)
                                                                  CCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGA                                                                                   >  SRR19973077.2788364/1‑150 (MQ=60)
                                                                   CATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAA                                                                                  <  SRR19973077.1891104/150‑1 (MQ=60)
                                                                   CATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAA                                                                                  >  SRR19973077.3565374/1‑150 (MQ=60)
                                                                   CATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTGTAGAAGCTTTTGAA                                                                                  >  SRR19973077.3565237/1‑150 (MQ=60)
                                                                    ATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAC                                                                                 >  SRR19973077.1625990/1‑150 (MQ=60)
                                                                    ATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAC                                                                                 <  SRR19973077.3977015/150‑1 (MQ=60)
                                                                    ATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAAC                                                                                 >  SRR19973077.1817920/1‑150 (MQ=60)
                                                                     TTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACA                                                                                <  SRR19973077.699448/150‑1 (MQ=60)
                                                                      TAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAA                                                                               >  SRR19973077.3164460/1‑150 (MQ=60)
                                                                      TAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAA                                                                               >  SRR19973077.574102/1‑150 (MQ=60)
                                                                       AGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAA                                                                              >  SRR19973077.521157/1‑150 (MQ=60)
                                                                       AGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAA                                                                              <  SRR19973077.2455533/150‑1 (MQ=60)
                                                                        GTCAGACGACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAAT                                                                             <  SRR19973077.4516785/150‑1 (MQ=60)
                                                                          CAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTA                                                                           <  SRR19973077.3047282/150‑1 (MQ=60)
                                                                                ACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTA                                                                     >  SRR19973077.2083247/1‑150 (MQ=60)
                                                                                 CATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTAC                                                                    <  SRR19973077.319103/150‑1 (MQ=60)
                                                                                    GTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTA                                                                 <  SRR19973077.3645062/150‑1 (MQ=60)
                                                                                     TTTGAAGAATGACTATTCATGACAAAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAA                                                                <  SRR19973077.1365739/150‑1 (MQ=60)
                                                                                      TTGAAGAATGACTATTCATGACACAAATATGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAAT                                                               <  SRR19973077.282946/150‑1 (MQ=60)
                                                                                        GAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTC                                                             <  SRR19973077.5156019/150‑1 (MQ=60)
                                                                                            AATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAA                                                         <  SRR19973077.3082766/150‑1 (MQ=60)
                                                                                              TGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATT                                                       >  SRR19973077.4812150/1‑150 (MQ=60)
                                                                                                ACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAA                                                     >  SRR19973077.4605554/1‑150 (MQ=60)
                                                                                                ACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAA                                                     >  SRR19973077.3351424/1‑150 (MQ=60)
                                                                                                   ATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTA                                                  >  SRR19973077.2217250/1‑150 (MQ=60)
                                                                                                     TCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAA                                                >  SRR19973077.434886/1‑150 (MQ=60)
                                                                                                     TCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAA                                                >  SRR19973077.434745/1‑150 (MQ=60)
                                                                                                      CATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAA                                               >  SRR19973077.149276/1‑150 (MQ=60)
                                                                                                       ATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAA                                              <  SRR19973077.211453/150‑1 (MQ=60)
                                                                                                        TGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAAT                                             <  SRR19973077.395161/150‑1 (MQ=60)
                                                                                                        TGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAAT                                             <  SRR19973077.2812581/150‑1 (MQ=60)
                                                                                                         GACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATA                                            >  SRR19973077.5054323/1‑150 (MQ=60)
                                                                                                         GACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATA                                            >  SRR19973077.3106494/1‑150 (MQ=60)
                                                                                                         GACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATA                                            >  SRR19973077.5054460/1‑150 (MQ=60)
                                                                                                         GACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATA                                            >  SRR19973077.2720916/1‑150 (MQ=60)
                                                                                                         GACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATA                                            >  SRR19973077.2720810/1‑150 (MQ=60)
                                                                                                            ACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGT                                         <  SRR19973077.1189184/150‑1 (MQ=60)
                                                                                                            ACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGT                                         <  SRR19973077.1188816/150‑1 (MQ=60)
                                                                                                            ACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGT                                         <  SRR19973077.1188862/150‑1 (MQ=60)
                                                                                                             CAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTT                                        <  SRR19973077.4859697/150‑1 (MQ=60)
                                                                                                              AAATAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAATTTC                                       >  SRR19973077.2023401/1‑150 (MQ=60)
                                                                                                               AATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCA                                      <  SRR19973077.156319/150‑1 (MQ=60)
                                                                                                                 TAGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACA                                    >  SRR19973077.4933271/1‑150 (MQ=60)
                                                                                                                  AGGAGAAACAAATGTTAGATATTAATGATCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAA                                   <  SRR19973077.3870240/150‑1 (MQ=60)
                                                                                                                  AGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAA                                   <  SRR19973077.788520/150‑1 (MQ=60)
                                                                                                                  AGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAA                                   <  SRR19973077.3870220/150‑1 (MQ=60)
                                                                                                                  AGGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGAACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAA                                   <  SRR19973077.3400911/150‑1 (MQ=60)
                                                                                                                   GGAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAG                                  >  SRR19973077.1436541/1‑150 (MQ=60)
                                                                                                                    GAGAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGT                                 <  SRR19973077.3084792/150‑1 (MQ=60)
                                                                                                                      GAAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGC                               >  SRR19973077.521580/1‑150 (MQ=60)
                                                                                                                       AAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCA                              <  SRR19973077.3293008/150‑1 (MQ=60)
                                                                                                                       AAACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCA                              <  SRR19973077.3292873/150‑1 (MQ=60)
                                                                                                                        AACAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAA                             <  SRR19973077.3227365/150‑1 (MQ=60)
                                                                                                                          CAAATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATT                           <  SRR19973077.4375736/150‑1 (MQ=60)
                                                                                                                           AAATGTAAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTG                          >  SRR19973077.1546117/1‑150 (MQ=60)
                                                                                                                            AATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGG                         >  SRR19973077.621513/1‑150 (MQ=60)
                                                                                                                            AATGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGG                         >  SRR19973077.4674980/1‑150 (MQ=60)
                                                                                                                             ATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGT                        >  SRR19973077.3655124/1‑150 (MQ=60)
                                                                                                                              TGTTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTT                       <  SRR19973077.110197/150‑1 (MQ=60)
                                                                                                                                TTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAG                     >  SRR19973077.2207137/1‑150 (MQ=60)
                                                                                                                                TTAGATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAG                     >  SRR19973077.2207437/1‑150 (MQ=60)
                                                                                                                                    ATATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTA                 >  SRR19973077.2017714/1‑150 (MQ=60)
                                                                                                                                     TATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTAT                >  SRR19973077.3247770/1‑150 (MQ=60)
                                                                                                                                      ATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATG               >  SRR19973077.4271033/1‑150 (MQ=60)
                                                                                                                                      ATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATG               <  SRR19973077.4038681/150‑1 (MQ=60)
                                                                                                                                      ATTAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGGTAGGGTATG               >  SRR19973077.851852/1‑150 (MQ=60)
                                                                                                                                        TAATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGA             >  SRR19973077.4915419/1‑150 (MQ=60)
                                                                                                                                          ATGAGTAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTAGCCTATTGTTCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGA           <  SRR19973077.4384952/150‑1 (MQ=60)
                                                                                                                                          ATGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGATA           >  SRR19973077.2293747/1‑150 (MQ=60)
                                                                                                                                           TGAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGAT          <  SRR19973077.688192/150‑1 (MQ=60)
                                                                                                                                            GAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATG         <  SRR19973077.3274593/150‑1 (MQ=60)
                                                                                                                                            GAGCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCATATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATG         <  SRR19973077.3274671/150‑1 (MQ=60)
                                                                                                                                              GCAATTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGG       >  SRR19973077.3287803/1‑150 (MQ=60)
                                                                                                                                              GCAATTATATTTGTTACCCAAATTTACAACCAGTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGG       <  SRR19973077.916783/150‑1 (MQ=60)
                                                                                                                                                  TTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTG   >  SRR19973077.3676058/1‑150 (MQ=60)
                                                                                                                                                  TTATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGAGTG   >  SRR19973077.3675927/1‑150 (MQ=60)
                                                                                                                                                   TATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  <  SRR19973077.352527/150‑1 (MQ=60)
                                                                                                                                                   TATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  <  SRR19973077.352581/150‑1 (MQ=60)
                                                                                                                                                   TATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  <  SRR19973077.352825/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TCGCCAGCATAAGACCAGACAGGGCAAAACCTAATTTTTTAGACATAAAAATCCTTTAATAAAATTCCATTAGTCAGACTACATGTTTGAAGAATGACTATTCATGACACAAATAGGAGAAACAAATGTTAGATATTAATGAGCAATGATATTTGTTACCCAAATTTACAACCATTGTTCATTAGGTCGCCTATTGTGCACTTTAGAAGCTTTTGAACAAATTAAATTTACTTAATTCAAAATTAAGTAAAAATAAGTTCACAAGTGCAATTGGTTAGGGTATGGAGATGGGATTGA  >  CP009273/153325‑153621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑