Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,334:1 +T 100% pseudogene (239/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,3341.T99.0% 683.2 / 0.7 207pseudogene (239/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (123/82);  total (123/84)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 232 total aligned reads displayed.

TTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGA  >  CP009273/524188‑524476
                                                                                                                                                   |                                                                                                                                              
   ataCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttt                                                                                                                                            <  1:289619/148‑1 (MQ=255)
      cAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttt                                                                                                                                         <  1:497646/148‑1 (MQ=255)
      cAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttt                                                                                                                                         <  1:2028026/148‑1 (MQ=255)
       aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttttt                                                                                                                                        <  1:1262599/148‑2 (MQ=255)
       aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttttt                                                                                                                                        <  2:449067/148‑2 (MQ=255)
       aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttttt                                                                                                                                        <  1:2223552/148‑2 (MQ=255)
        aaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAAAACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttc                                                                                                                                       >  1:3250167/1‑146 (MQ=255)
         aGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttct                                                                                                                                      >  2:826407/1‑145 (MQ=255)
          taTGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctt                                                                                                                                     <  2:105410/147‑5 (MQ=255)
            tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAt                                                                                                                                    <  1:2044746/148‑1 (MQ=255)
             gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAtt                                                                                                                                   >  2:287464/1‑148 (MQ=255)
              aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                  <  1:1140844/148‑1 (MQ=255)
              aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                  <  2:2994437/148‑1 (MQ=255)
              aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAACATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                  <  2:2258822/148‑1 (MQ=255)
               aTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAtttt                                                                                                                                 <  2:671688/148‑1 (MQ=255)
               aTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAtttt                                                                                                                                 >  2:503775/1‑148 (MQ=255)
                tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttttt                                                                                                                                >  2:2602192/1‑148 (MQ=255)
                tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttttt                                                                                                                                >  1:2468923/1‑148 (MQ=255)
                tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttttt                                                                                                                                <  2:3127133/148‑1 (MQ=255)
                tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTATTAttttt                                                                                                                                >  1:2469455/1‑148 (MQ=255)
                   ctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTccc                                                                                                                             >  1:1067512/1‑148 (MQ=255)
                   ctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTccc                                                                                                                             <  1:3036370/148‑1 (MQ=255)
                       taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGGTGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAAc                                                                                                                         >  1:1575587/1‑148 (MQ=255)
                       taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAAc                                                                                                                         >  1:2769015/1‑148 (MQ=255)
                       taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCACTAAc                                                                                                                         >  2:1596110/1‑148 (MQ=255)
                          gtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACaaa                                                                                                                      >  1:1670618/1‑148 (MQ=255)
                            aaaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatg                                                                                                                    >  2:1716238/1‑148 (MQ=255)
                              aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgat                                                                                                                  >  1:1085522/1‑148 (MQ=255)
                                 ttACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                               >  2:1088708/1‑148 (MQ=255)
                                  tACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgatg                                                                                                              >  1:499191/1‑148 (MQ=255)
                                       aaaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                         <  1:3158417/148‑1 (MQ=255)
                                       aaaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                         <  1:63481/148‑1 (MQ=255)
                                            atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                    <  1:753799/148‑1 (MQ=255)
                                            atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                    <  1:11099/148‑1 (MQ=255)
                                            atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAACATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                    >  1:1976805/1‑148 (MQ=255)
                                             tatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGttt                                                                                                   >  2:1303566/1‑148 (MQ=255)
                                                    aTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                            <  2:1963499/148‑1 (MQ=255)
                                                    aTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                            <  2:2124255/148‑1 (MQ=255)
                                                     tACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAAc                                                                                           <  1:3000096/148‑1 (MQ=255)
                                                     tACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAAc                                                                                           >  1:329876/1‑148 (MQ=255)
                                                      aCCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACa                                                                                          >  2:3165957/1‑148 (MQ=255)
                                                      aCCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACa                                                                                          <  2:2805929/148‑1 (MQ=255)
                                                      aCCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACa                                                                                          <  2:2805720/148‑1 (MQ=255)
                                                        cAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACAtt                                                                                        >  1:1535354/1‑148 (MQ=255)
                                                         aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTg                                                                                       <  2:1235625/148‑1 (MQ=255)
                                                         aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTg                                                                                       <  1:419608/148‑1 (MQ=255)
                                                         aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTg                                                                                       <  2:2698234/148‑1 (MQ=255)
                                                         aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTg                                                                                       <  1:199609/148‑1 (MQ=255)
                                                          gAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa                                                                                      >  1:1704305/1‑148 (MQ=255)
                                                           aaaaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                     >  1:1618566/1‑148 (MQ=255)
                                                            aaaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACa                                                                                    >  1:2980186/1‑148 (MQ=255)
                                                              atgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGt                                                                                  >  2:3217883/1‑148 (MQ=255)
                                                               tgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg                                                                                 <  1:2314685/148‑1 (MQ=255)
                                                               tgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg                                                                                 <  1:1394759/148‑1 (MQ=255)
                                                                 atgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAg                                                                               <  2:1952343/148‑1 (MQ=255)
                                                                   ggAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAggg                                                                             >  1:108408/1‑148 (MQ=255)
                                                                        ttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                        >  1:845330/1‑148 (MQ=255)
                                                                         tttACATGGCTCCCAGATTTTTGTCCGCACGTAGCGGTTGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAggg                                                                       <  2:2054484/148‑1 (MQ=255)
                                                                             cATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                   >  1:798830/1‑148 (MQ=255)
                                                                             cATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                   >  2:2589507/1‑148 (MQ=255)
                                                                              aTGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACaa                                                                  >  1:405612/1‑148 (MQ=255)
                                                                                ggCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGt                                                                >  1:3076305/1‑148 (MQ=255)
                                                                                 gCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                               <  1:866321/148‑1 (MQ=255)
                                                                                      cAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaa                                                          >  1:767951/1‑148 (MQ=255)
                                                                                             ttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTa                                                   <  2:1274129/148‑1 (MQ=255)
                                                                                             ttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTa                                                   <  1:476996/148‑1 (MQ=255)
                                                                                              tttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                  <  1:3195691/148‑1 (MQ=255)
                                                                                               ttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATc                                                 <  1:242003/148‑1 (MQ=255)
                                                                                                    cACGTAGCGTTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGa                                            <  2:3244411/148‑1 (MQ=255)
                                                                                                      cGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAAt                                          <  2:1085522/148‑1 (MQ=255)
                                                                                                        tAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGa                                        <  1:2729744/148‑1 (MQ=255)
                                                                                                             gTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAg                                   <  2:2457308/148‑1 (MQ=255)
                                                                                                              ttgATATATAAATATACACATATGTATAATATGATTATTTTTTTTCTTATTTTTCAATAACAAATGATGATGGGGGTAGTTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGa                                  <  2:2916814/146‑1 (MQ=255)
                                                                                                               ggATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGAt                                 >  1:301498/1‑148 (MQ=255)
                                                                                                                  tatatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACa                              >  2:1877517/1‑148 (MQ=255)
                                                                                                                   atatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaa                             >  2:3102999/1‑148 (MQ=255)
                                                                                                                     atCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaa                           >  2:928426/1‑148 (MQ=255)
                                                                                                                     atCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaa                           >  2:554386/1‑148 (MQ=255)
                                                                                                                     atCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaa                           >  1:351862/1‑148 (MQ=255)
                                                                                                                      tCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa                          >  1:1786612/1‑148 (MQ=255)
                                                                                                                       cAATATACACAAATGTAGAAGATGATTATTTTTTTTATTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTTAGGGCCAGGGAAAAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaa                         >  2:3311376/1‑148 (MQ=255)
                                                                                                                         atatACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAg                       <  2:493222/148‑1 (MQ=255)
                                                                                                                           ataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtg                     >  2:2191099/1‑148 (MQ=255)
                                                                                                                            taCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtgt                    <  1:2589507/148‑1 (MQ=255)
                                                                                                                             acacAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGtgtg                   >  2:732293/1‑148 (MQ=255)
                                                                                                                               acaAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggt                 <  1:3217883/148‑1 (MQ=255)
                                                                                                                                caAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggtg                <  1:568830/148‑1 (MQ=255)
                                                                                                                                 aaaTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGgtgt               <  1:287464/148‑1 (MQ=255)
                                                                                                                                 aaaTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGgtgt               <  1:208061/148‑1 (MQ=255)
                                                                                                                                   aTGTAGAATATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaa             <  2:2114803/148‑1 (MQ=255)
                                                                                                                                     gTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat           >  2:2380671/1‑148 (MQ=255)
                                                                                                                                     gTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGGGTGGTGTAAat           >  2:2027151/1‑148 (MQ=255)
                                                                                                                                        gaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATg        >  2:1364480/1‑148 (MQ=255)
                                                                                                                                        gaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATg        <  1:2228546/148‑1 (MQ=255)
                                                                                                                                          agaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCTTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGt      >  1:89426/1‑148 (MQ=255)
                                                                                                                                           gatgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGGTGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTa     <  2:2574675/148‑1 (MQ=255)
                                                                                                                                           gatgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTa     >  2:1852768/1‑148 (MQ=255)
                                                                                                                                            atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTaa    >  2:2271654/1‑148 (MQ=255)
                                                                                                                                              gatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa  >  2:1538795/1‑148 (MQ=255)
                                                                                                                                              gatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAAAAAAAAAGTGTGGTGTAAATATGGTAAGa  >  2:3290541/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGA  >  CP009273/524188‑524476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A