Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,334:1 +T 100% pseudogene (239/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,3341.T98.8% 539.5 / ‑0.1 162pseudogene (239/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (90/70);  total (90/72)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 179 total aligned reads displayed.

TTATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524185‑524478
                                                                                                                                                       |                                                                                                                                                
ttatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatt‑a                                                                                                                                                   <  1:4003518/150‑1 (MQ=255)
 tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatt‑a‑t                                                                                                                                                 >  2:1868836/1‑150 (MQ=255)
 tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTACGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatt‑a‑t                                                                                                                                                 >  1:4434710/1‑150 (MQ=255)
   tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑ttt                                                                                                                                               <  2:3494111/150‑1 (MQ=255)
   tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑ttt                                                                                                                                               <  2:1323128/150‑1 (MQ=255)
    ttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑tttt                                                                                                                                              >  2:3707051/1‑150 (MQ=255)
     tataCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑ttttt                                                                                                                                             >  1:1954837/1‑150 (MQ=255)
      ataCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑tttttt                                                                                                                                            >  1:447006/1‑150 (MQ=255)
          aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑ttttttttct                                                                                                                                        >  1:3082263/1‑147 (MQ=255)
          aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑ttttttttct                                                                                                                                        <  1:2421036/150‑4 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAttt                                                                                                                                    >  1:3255598/1‑150 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAttt                                                                                                                                    >  1:2882943/1‑150 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAttt                                                                                                                                    <  2:3295449/150‑1 (MQ=255)
                gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAtttt                                                                                                                                   <  2:130905/150‑1 (MQ=255)
                gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAtttt                                                                                                                                   >  1:4623759/1‑150 (MQ=255)
                gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAtttt                                                                                                                                   >  2:2478594/1‑150 (MQ=255)
                gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAtttt                                                                                                                                   >  1:4623773/1‑150 (MQ=255)
                 aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAttttt                                                                                                                                  <  2:2521203/150‑1 (MQ=255)
                 aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTAttttt                                                                                                                                  >  1:729663/1‑150 (MQ=255)
                  aTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTc                                                                                                                                 >  2:1377719/1‑150 (MQ=255)
                      ctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTa                                                                                                                             <  1:3663775/150‑1 (MQ=255)
                         gtaagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACa                                                                                                                          <  1:3714347/150‑1 (MQ=255)
                          taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACaa                                                                                                                         >  2:1403633/1‑150 (MQ=255)
                           aagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACaaa                                                                                                                        >  2:3795055/1‑150 (MQ=255)
                           aagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACaaa                                                                                                                        <  2:3094857/150‑1 (MQ=255)
                            agtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAat                                                                                                                       <  1:1536250/150‑1 (MQ=255)
                            agtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAat                                                                                                                       <  2:3065391/150‑1 (MQ=255)
                              taaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAatga                                                                                                                     <  2:1792756/150‑1 (MQ=255)
                              taaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAatga                                                                                                                     >  1:1542219/1‑150 (MQ=255)
                               aaaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAatgat                                                                                                                    >  2:5126277/1‑150 (MQ=255)
                                  cATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                 <  2:2146892/150‑1 (MQ=255)
                                  cATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                 <  2:2146931/150‑1 (MQ=255)
                                        aTAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                           <  1:3263808/150‑1 (MQ=255)
                                        aTAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                           <  1:3229295/150‑1 (MQ=255)
                                          aaaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAg                                                                                                         >  1:96262/1‑150 (MQ=255)
                                          aaaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAg                                                                                                         >  1:96294/1‑150 (MQ=255)
                                           aaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAgg                                                                                                        >  1:2560170/1‑150 (MQ=255)
                                            aCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGt                                                                                                       <  2:1582533/150‑1 (MQ=255)
                                             cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                      >  2:2916741/1‑150 (MQ=255)
                                             cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                      <  1:4321980/150‑1 (MQ=255)
                                              tataTGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGttt                                                                                                     >  2:1738653/1‑150 (MQ=255)
                                               atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTa                                                                                                    >  2:3746598/1‑150 (MQ=255)
                                                 atGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAg                                                                                                  >  2:256604/1‑150 (MQ=255)
                                                  tGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGa                                                                                                 >  1:4515147/1‑150 (MQ=255)
                                                    gCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaa                                                                                               >  2:1719929/1‑150 (MQ=255)
                                                    gCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaa                                                                                               <  2:3861441/150‑1 (MQ=255)
                                                      aaTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAAc                                                                                             >  1:3012668/1‑150 (MQ=255)
                                                           cAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa                                                                                        >  2:4441371/1‑150 (MQ=255)
                                                           cAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa                                                                                        >  1:3562723/1‑150 (MQ=255)
                                                            aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTGTAAGAAACCATTGAc                                                                                       >  2:1120790/1‑150 (MQ=255)
                                                                  tgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAg                                                                                 >  1:1156053/1‑150 (MQ=255)
                                                                  tgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGACGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAg                                                                                 >  1:1156187/1‑150 (MQ=255)
                                                                   gatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAATACATTGACAGTGAgg                                                                                >  1:2943923/1‑150 (MQ=255)
                                                                    atgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTAA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAggg                                                                               <  2:4747685/150‑1 (MQ=255)
                                                                     tgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGc                                                                              >  2:657324/1‑150 (MQ=255)
                                                                     tgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGc                                                                              <  1:2487597/150‑1 (MQ=255)
                                                                       gAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACTTAGCGGTGGATATATAAATATAGTCAAATGTATAACATGATT‑ATTTTTTTTCTTATTTTTACCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCa                                                                            <  1:2958647/150‑1 (MQ=255)
                                                                         gTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                          >  1:3418390/1‑150 (MQ=255)
                                                                         gTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                          <  1:2335789/150‑1 (MQ=255)
                                                                         gTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                          <  1:1725873/150‑1 (MQ=255)
                                                                          tttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAggg                                                                         <  2:4628456/150‑1 (MQ=255)
                                                                           ttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGa                                                                        >  1:4173092/1‑150 (MQ=255)
                                                                             ttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAAc                                                                      <  1:3722934/150‑1 (MQ=255)
                                                                             ttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAAc                                                                      <  1:1261436/150‑1 (MQ=255)
                                                                              tACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                     <  2:3183825/150‑1 (MQ=255)
                                                                                cATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAg                                                                   <  1:3771545/150‑1 (MQ=255)
                                                                                cATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAg                                                                   <  1:2183829/150‑1 (MQ=255)
                                                                                  tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 >  1:1372437/1‑150 (MQ=255)
                                                                                  tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 <  1:4962269/150‑1 (MQ=255)
                                                                                         cAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaaaa                                                          >  1:3369426/1‑150 (MQ=255)
                                                                                           gATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATc                                                        <  2:4348570/150‑1 (MQ=255)
                                                                                            aTTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCg                                                       <  2:3416536/150‑1 (MQ=255)
                                                                                            aTTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCg                                                       <  2:3416567/150‑1 (MQ=255)
                                                                                              ttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTa                                                     <  2:4255088/150‑1 (MQ=255)
                                                                                                  ttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTATGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCaa                                                 >  1:4365683/1‑150 (MQ=255)
                                                                                                   tCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCaaa                                                <  2:325213/150‑1 (MQ=255)
                                                                                                         cGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGa                                          >  1:5116790/1‑150 (MQ=255)
                                                                                                          gTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGAt                                         <  2:2527990/150‑1 (MQ=255)
                                                                                                            aGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAGTGATcc                                       >  1:1461427/1‑150 (MQ=255)
                                                                                                            aGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAGTGATcc                                       >  1:1461383/1‑150 (MQ=255)
                                                                                                            aGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATcc                                       <  2:4269882/150‑1 (MQ=255)
                                                                                                               ggTGGATATATCAATATACACAAATTTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGTATGATCCAGa                                    <  2:5007343/150‑1 (MQ=255)
                                                                                                                 tGGATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATa                                  <  1:1868930/150‑1 (MQ=255)
                                                                                                                  ggATATATCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAc                                 >  2:1165854/1‑150 (MQ=255)
                                                                                                                       tatCAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa                            >  2:949866/1‑150 (MQ=255)
                                                                                                                          cAATATACACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAg                         <  2:969601/150‑1 (MQ=255)
                                                                                                                              ataCACAAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGtgtg                     <  2:318634/150‑1 (MQ=255)
                                                                                                                                 cacaAATGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggtg                  <  2:663458/150‑1 (MQ=255)
                                                                                                                                    aaaTGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaa               >  2:3793570/1‑150 (MQ=255)
                                                                                                                                    aaaTGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaa               >  2:1429623/1‑150 (MQ=255)
                                                                                                                                      aTGTAGAAGATGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat             >  2:811035/1‑150 (MQ=255)
                                                                                                                                          agaagaTGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAAGAAGTGGCGAAAATCGTATCTAAGAATGATCGAGATACAAAAAAAGTGTGGTGTACATATgg         >  2:5088174/1‑150 (MQ=255)
                                                                                                                                          agaagaTGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg         >  2:4033871/1‑150 (MQ=255)
                                                                                                                                          agaagaTGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg         <  2:2246637/150‑1 (MQ=255)
                                                                                                                                          agaagaTGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg         >  1:3602865/1‑150 (MQ=255)
                                                                                                                                          agaagaTGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg         >  1:200467/1‑150 (MQ=255)
                                                                                                                                             agaTGATT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTGAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGGGGTGGAAATATGGTaa      >  2:4550940/1‑150 (MQ=255)
                                                                                                                                                tgatT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt   >  1:3514400/1‑150 (MQ=255)
                                                                                                                                                tgatT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt   >  1:2121873/1‑150 (MQ=255)
                                                                                                                                                 gatT‑ATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAtc  >  2:27274/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
TTATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATT‑A‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524185‑524478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A