Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,334:1 +T 100% pseudogene (239/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,3341.T85.7% 719.7 / 140.2 238pseudogene (239/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base . (19/15);  new base T (122/82);  total (141/97)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 274 total aligned reads displayed.

TTATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524185‑524478
                                                                                                                                                      |                                                                                                                                                  
ttatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta                                                                                                                                                     <  2:211277/150‑1 (MQ=255)
 tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t                                                                                                                                                   >  2:615913/1‑150 (MQ=255)
 tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t                                                                                                                                                   >  2:2598904/1‑150 (MQ=255)
  atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATATACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t‑t                                                                                                                                                 <  1:871766/150‑1 (MQ=255)
  atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGttta‑t‑t                                                                                                                                                 >  1:2614690/1‑150 (MQ=255)
  atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t‑t                                                                                                                                                 >  1:622946/1‑150 (MQ=255)
  atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t‑t                                                                                                                                                 >  1:2897180/1‑150 (MQ=255)
   tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑t‑tt                                                                                                                                                >  2:4217098/1‑150 (MQ=255)
       taCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑t‑tttt‑tt                                                                                                                                           >  1:327767/1‑150 (MQ=255)
        aCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑t‑tttt‑ttt                                                                                                                                          <  2:404656/150‑2 (MQ=255)
         cAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑t‑tttt‑tttc                                                                                                                                         <  2:3075505/150‑3 (MQ=255)
         cAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACAAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTATCCGCACGTAGCGGTGGATATATCAAAAAACACAAAAGTAGACGATGATTA‑t‑tttt‑ttta                                                                                                                                         >  1:3782841/1‑148 (MQ=255)
            aGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑t‑tttt‑tttctta                                                                                                                                      <  2:1995049/150‑6 (MQ=255)
             gATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTAt                                                                                                                                      >  2:912272/1‑150 (MQ=255)
             gATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTAtt                                                                                                                                     >  2:4175661/1‑150 (MQ=255)
             gATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTAtt                                                                                                                                     >  2:4175830/1‑150 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTAttt                                                                                                                                    <  1:2186520/150‑1 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTAttt                                                                                                                                    >  2:1135594/1‑150 (MQ=255)
                  aTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTc                                                                                                                                 <  1:2154389/150‑1 (MQ=255)
                      ctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTa                                                                                                                             <  2:1587483/150‑1 (MQ=255)
                       ttGTAATTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTaa                                                                                                                            <  2:3480561/150‑1 (MQ=255)
                       ttGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTaa                                                                                                                            <  1:722983/150‑1 (MQ=255)
                            agtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAatg                                                                                                                      <  2:2220382/150‑1 (MQ=255)
                              taaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAatga                                                                                                                     >  2:1335756/1‑150 (MQ=255)
                               aaaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAatgat                                                                                                                    <  2:2238084/150‑1 (MQ=255)
                               aaaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAatgat                                                                                                                    >  2:2853258/1‑150 (MQ=255)
                                aaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAatgatg                                                                                                                   >  2:263043/1‑150 (MQ=255)
                                       cATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATggggg                                                                                                            >  1:3743001/1‑150 (MQ=255)
                                        aTAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                           >  1:3315582/1‑150 (MQ=255)
                                              tataTGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGTTGGGGGTAGGttt                                                                                                     >  1:4322162/1‑150 (MQ=255)
                                               atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTaa                                                                                                   <  2:1155776/150‑1 (MQ=255)
                                                tatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTaa                                                                                                   >  1:2163163/1‑150 (MQ=255)
                                                tatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTaa                                                                                                   >  1:2164145/1‑150 (MQ=255)
                                                tatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTaa                                                                                                   >  1:2556783/1‑150 (MQ=255)
                                                tatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGAGGAGGGGGGTAGGTTTat                                                                                                   >  1:316980/1‑149 (MQ=255)
                                                  tGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGa                                                                                                 >  2:2167629/1‑150 (MQ=255)
                                                     cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                              >  2:2774950/1‑150 (MQ=255)
                                                     cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                              >  1:2553049/1‑150 (MQ=255)
                                                     cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                              >  2:2224364/1‑150 (MQ=255)
                                                     cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TATTTT‑TTCTTATTTTTCCCTAAAAAATGATGATGTTGGTATGTTTAATaaaa                                                                                              >  2:4461423/1‑150 (MQ=255)
                                                      aaTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACa                                                                                            >  2:3654936/1‑150 (MQ=255)
                                                       aTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACAt                                                                                           >  2:1603251/1‑150 (MQ=255)
                                                         aCCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACAtt                                                                                          <  1:1626130/150‑1 (MQ=255)
                                                           cAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa                                                                                        >  2:324727/1‑150 (MQ=255)
                                                           cAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa                                                                                        >  2:2496360/1‑150 (MQ=255)
                                                            aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  1:2832961/1‑150 (MQ=255)
                                                                aaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg                                                                                   >  2:1805499/1‑150 (MQ=255)
                                                                 atgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGa                                                                                  >  1:1324507/1‑150 (MQ=255)
                                                                       gAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCa                                                                            <  1:2274567/150‑1 (MQ=255)
                                                                       gAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCa                                                                            >  2:1465232/1‑150 (MQ=255)
                                                                          tttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCATAACAAATGATGATGGGGGTAGGGTTAAGAAAAAATTGACAGTGAGGGCCAggg                                                                         >  2:2952783/1‑150 (MQ=255)
                                                                              tACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                     >  1:342976/1‑150 (MQ=255)
                                                                              tACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGGTTAAGAAAACCTTGACAGTGAGGGCCAGGGAACa                                                                     >  2:4410278/1‑150 (MQ=255)
                                                                                 aTGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGt                                                                  >  1:3530496/1‑150 (MQ=255)
                                                                                 aTGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGt                                                                  <  1:263043/150‑1 (MQ=255)
                                                                                    gCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGc                                                               >  2:2360950/1‑150 (MQ=255)
                                                                                      tCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGa                                                             >  1:4488221/1‑150 (MQ=255)
                                                                                         cAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaaaa                                                          <  1:3613648/150‑1 (MQ=255)
                                                                                          aGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAAt                                                         <  1:2496360/150‑1 (MQ=255)
                                                                                             tttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGAGAGGGCCAGGGAACAAGTGGCGAAAATCGt                                                      <  1:242373/150‑1 (MQ=255)
                                                                                              ttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTATTATTTTTCCCTAACAAATGATGATGGGGGTTGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                    <  1:2929425/150‑1 (MQ=255)
                                                                                               tttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                    <  1:2132989/150‑1 (MQ=255)
                                                                                                ttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATc                                                   <  2:3293243/150‑1 (MQ=255)
                                                                                                  ttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGACCAAGTGGCGAAAATCGTATCaa                                                 >  2:879662/1‑150 (MQ=255)
                                                                                                     cGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGa                                              >  2:297341/1‑150 (MQ=255)
                                                                                                     cGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGaa                                             <  1:3944299/150‑1 (MQ=255)
                                                                                                         cGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGa                                          <  2:3743001/150‑1 (MQ=255)
                                                                                                           tAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATc                                        <  2:1739762/150‑1 (MQ=255)
                                                                                                           tAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATc                                        <  2:2056114/150‑1 (MQ=255)
                                                                                                           tAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATc                                        >  1:1489105/1‑150 (MQ=255)
                                                                                                            aGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGACCAAGTGGCGAAAATCGTATCAAAGAATGATcc                                       >  1:3674487/1‑150 (MQ=255)
                                                                                                               ggTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGa                                    >  2:3178243/1‑150 (MQ=255)
                                                                                                                gTGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGAt                                   >  2:1897926/1‑150 (MQ=255)
                                                                                                                 tGGATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATa                                  <  1:1773871/150‑1 (MQ=255)
                                                                                                                  ggATATATCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAc                                 <  1:1291131/150‑1 (MQ=255)
                                                                                                                    atatatCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaa                               <  1:537185/150‑1 (MQ=255)
                                                                                                                    atatatCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaa                               <  1:2822243/150‑1 (MQ=255)
                                                                                                                    atatatCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaa                              >  2:1361891/1‑150 (MQ=255)
                                                                                                                        atCAATATACACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaa                           >  1:2241099/1‑150 (MQ=255)
                                                                                                                             tataCACAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtgt                      >  1:3729605/1‑150 (MQ=255)
                                                                                                                                acacAAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggt                   <  1:932889/150‑1 (MQ=255)
                                                                                                                                acacAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggtg                  >  2:549711/1‑150 (MQ=255)
                                                                                                                                 cacaAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggtg                  >  1:222117/1‑150 (MQ=255)
                                                                                                                                 cacaAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggtg                  >  1:4541538/1‑150 (MQ=255)
                                                                                                                                  acaAATGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGgtgt                 <  1:1391907/150‑1 (MQ=255)
                                                                                                                                    aaaTGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaa               <  2:1127721/150‑1 (MQ=255)
                                                                                                                                     aaTGTAGAAGATGATTATT‑TTTT‑TTCTTATCTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaaa              >  2:2678567/1‑150 (MQ=255)
                                                                                                                                      aTGTAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat             >  1:794076/1‑150 (MQ=255)
                                                                                                                                         tAGAAGATGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATg          >  1:3379509/1‑150 (MQ=255)
                                                                                                                                           gaagaTGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGt        >  1:1097244/1‑150 (MQ=255)
                                                                                                                                           gaagaTGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGGGTAAATATGGt        >  1:4327307/1‑150 (MQ=255)
                                                                                                                                             agaTGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTaa      >  1:4017459/1‑150 (MQ=255)
                                                                                                                                             agaTGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTaa      <  2:1209169/150‑1 (MQ=255)
                                                                                                                                             agaTGATTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTaa      <  2:1677863/150‑1 (MQ=255)
                                                                                                                                             agaTGATTA‑T‑TTTTGTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTaa      <  2:2475389/150‑1 (MQ=255)
                                                                                                                                             agaTGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAg     >  1:1287001/1‑150 (MQ=255)
                                                                                                                                              gatgatTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAg     >  1:1028556/1‑150 (MQ=255)
                                                                                                                                               atgatTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa    <  2:3379509/150‑1 (MQ=255)
                                                                                                                                               atgatTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt   >  1:4133999/1‑150 (MQ=255)
                                                                                                                                                 gatTATT‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATa  >  1:3244705/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
TTATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑T‑TTTT‑TTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524185‑524478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A