Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,334:1 +T 100% pseudogene (239/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,3341.T96.2% 698.6 / 21.7 209pseudogene (239/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base T (84/117);  total (87/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 237 total aligned reads displayed.

TTATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524185‑524478
                                                                                                                                                      |                                                                                                                                                
ttatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta                                                                                                                                                   <  1:520831/150‑1 (MQ=255)
 tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t                                                                                                                                                 <  1:2588489/150‑1 (MQ=255)
   tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttt                                                                                                                                               <  2:4463676/150‑1 (MQ=255)
      ataCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttt                                                                                                                                            <  2:854165/150‑1 (MQ=255)
        aCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttttt                                                                                                                                          >  1:2642362/1‑149 (MQ=255)
        aCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttttt                                                                                                                                          <  1:2572006/150‑2 (MQ=255)
        aCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttttt                                                                                                                                          <  1:2467039/150‑2 (MQ=255)
         cAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAAGGTAGAAGATGATTA‑ttttttttc                                                                                                                                         >  2:3975774/1‑148 (MQ=255)
           aaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctt                                                                                                                                       >  2:3531858/1‑146 (MQ=255)
           aaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctt                                                                                                                                       >  1:3634481/1‑146 (MQ=255)
           aaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctt                                                                                                                                       <  1:3469318/150‑5 (MQ=255)
           aaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctt                                                                                                                                       >  1:2180388/1‑146 (MQ=255)
            aGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctta                                                                                                                                      >  1:4404305/1‑145 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                    <  2:1351223/150‑1 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                    <  1:2784268/150‑1 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                    >  2:2614499/1‑150 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                    <  1:2484039/150‑1 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                    <  1:4235608/150‑1 (MQ=255)
               tGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttt                                                                                                                                    <  2:4417487/150‑1 (MQ=255)
                 aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttttt                                                                                                                                  <  2:2630268/150‑1 (MQ=255)
                       ttGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTaa                                                                                                                            <  1:1212978/150‑1 (MQ=255)
                          taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCGGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACaa                                                                                                                         <  1:804916/150‑1 (MQ=255)
                             gtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatg                                                                                                                      <  1:3489493/150‑1 (MQ=255)
                             gtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatg                                                                                                                      <  1:2915089/150‑1 (MQ=255)
                               aaaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgat                                                                                                                    <  1:4607159/150‑1 (MQ=255)
                                 aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                 >  2:2840442/1‑150 (MQ=255)
                                     tACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATggg                                                                                                              >  1:2363683/1‑150 (MQ=255)
                                     tACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATggg                                                                                                              <  1:1031627/150‑1 (MQ=255)
                                       cATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATggggg                                                                                                            >  2:2279343/1‑150 (MQ=255)
                                       cATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATggggg                                                                                                            >  2:2167613/1‑150 (MQ=255)
                                         tAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTa                                                                                                          <  2:600666/150‑1 (MQ=255)
                                             cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                      <  1:3242492/150‑1 (MQ=255)
                                                 atGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAg                                                                                                  <  1:1344648/150‑1 (MQ=255)
                                                 atGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAg                                                                                                  <  1:419175/150‑1 (MQ=255)
                                                      aaTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAAc                                                                                             <  1:1251332/150‑1 (MQ=255)
                                                       aTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACa                                                                                            <  2:1279566/150‑1 (MQ=255)
                                                            aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  1:1855600/1‑150 (MQ=255)
                                                             gAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACa                                                                                      <  2:1130147/150‑1 (MQ=255)
                                                              aaaaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAg                                                                                     >  1:1238884/1‑150 (MQ=255)
                                                               aaaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGt                                                                                    >  2:4340938/1‑150 (MQ=255)
                                                                aaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg                                                                                   >  2:507326/1‑150 (MQ=255)
                                                                  tgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAg                                                                                 <  2:3004643/150‑1 (MQ=255)
                                                                   gatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAgg                                                                                >  1:3939757/1‑150 (MQ=255)
                                                                    atgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAATAAAACATTGACAGTGAggg                                                                               >  1:2321039/1‑150 (MQ=255)
                                                                    atgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAggg                                                                               <  1:3328610/150‑1 (MQ=255)
                                                                    atgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAggg                                                                               <  2:1349449/150‑1 (MQ=255)
                                                                     tgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGc                                                                              >  2:3709424/1‑150 (MQ=255)
                                                                     tgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGc                                                                              >  2:1388915/1‑150 (MQ=255)
                                                                      ggAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGcc                                                                             >  2:65429/1‑150 (MQ=255)
                                                                        aGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGTGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAg                                                                           >  2:1000216/1‑150 (MQ=255)
                                                                         gTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                          <  1:1273142/150‑1 (MQ=255)
                                                                         gTTTTTACATGGCTACCAGATTTTTTTCCGCACGTAGCGGTGTATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                          <  1:1272826/150‑1 (MQ=255)
                                                                           ttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGa                                                                        <  2:4162796/150‑1 (MQ=255)
                                                                            tttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGaa                                                                       <  1:1744008/150‑1 (MQ=255)
                                                                              tACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                     <  2:3261869/150‑1 (MQ=255)
                                                                              tACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                     <  2:742715/150‑1 (MQ=255)
                                                                               aCATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGACCaa                                                                    >  2:1396211/1‑150 (MQ=255)
                                                                               aCATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACaa                                                                    <  1:1482442/150‑1 (MQ=255)
                                                                                cATGGCTCCCGGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAg                                                                   <  1:693703/150‑1 (MQ=255)
                                                                                 aTGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGt                                                                  <  1:2439011/150‑1 (MQ=255)
                                                                                  tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 <  2:1903178/150‑1 (MQ=255)
                                                                                  tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 <  2:2956185/150‑1 (MQ=255)
                                                                                    gCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGc                                                               <  1:56243/150‑1 (MQ=255)
                                                                                       cccAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaa                                                            >  2:331782/1‑150 (MQ=255)
                                                                                         cAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaaaa                                                          >  1:1412687/1‑150 (MQ=255)
                                                                                          aGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAAt                                                         >  1:3176841/1‑150 (MQ=255)
                                                                                           gATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAATATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATc                                                        <  2:1549461/150‑1 (MQ=255)
                                                                                             tttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGt                                                      >  1:1992219/1‑150 (MQ=255)
                                                                                              ttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTa                                                     <  2:2403560/150‑1 (MQ=255)
                                                                                                ttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATc                                                   >  2:148341/1‑150 (MQ=255)
                                                                                                       cACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAAt                                            <  2:632623/150‑1 (MQ=255)
                                                                                                       cACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAAt                                            >  2:3725088/1‑150 (MQ=255)
                                                                                                        aCGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATg                                           <  1:1376086/150‑1 (MQ=255)
                                                                                                        aCGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATg                                           <  1:2376006/150‑1 (MQ=255)
                                                                                                          gTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGAt                                         >  2:2234300/1‑150 (MQ=255)
                                                                                                            aGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATcc                                       >  2:3276969/1‑150 (MQ=255)
                                                                                                                gTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGAt                                   <  2:1828028/150‑1 (MQ=255)
                                                                                                                 tGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATa                                  <  1:3784451/150‑1 (MQ=255)
                                                                                                                     tatatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaa                              <  1:1189417/150‑1 (MQ=255)
                                                                                                                       tatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa                            >  1:914294/1‑150 (MQ=255)
                                                                                                                         tCAATATACACAACTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGACTGATCCAGATACaaaaaaa                          >  2:50336/1‑150 (MQ=255)
                                                                                                                         tCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaaa                          >  2:2368603/1‑150 (MQ=255)
                                                                                                                         tCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaaa                          >  1:1110664/1‑150 (MQ=255)
                                                                                                                          cAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAg                         >  2:2792637/1‑150 (MQ=255)
                                                                                                                              ataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCGGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGtgtg                     >  1:1078612/1‑150 (MQ=255)
                                                                                                                              ataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGtgtg                     >  2:1761560/1‑150 (MQ=255)
                                                                                                                               taCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtgg                    >  2:4540233/1‑150 (MQ=255)
                                                                                                                                acacAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtggt                   <  1:1743125/150‑1 (MQ=255)
                                                                                                                                  acaAATGTAGACGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGgtgt                 <  2:2896509/150‑1 (MQ=255)
                                                                                                                                  acaAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGTGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGgtgt                 <  2:1282694/150‑1 (MQ=255)
                                                                                                                                    aaaTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaa               >  1:3018749/1‑150 (MQ=255)
                                                                                                                                       tGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAata            >  2:3187264/1‑150 (MQ=255)
                                                                                                                                         tAGAAGATTATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAATTGGCGAAAATCGTATCTAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATg          <  1:3284774/150‑1 (MQ=255)
                                                                                                                                          agaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg         <  1:2530931/150‑1 (MQ=255)
                                                                                                                                          agaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg         <  1:4589213/150‑1 (MQ=255)
                                                                                                                                           gaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGt        >  1:3133632/1‑150 (MQ=255)
                                                                                                                                           gaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGt        <  2:2642362/150‑1 (MQ=255)
                                                                                                                                              gatgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAg     <  2:4628611/150‑1 (MQ=255)
                                                                                                                                               atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa    >  1:2658239/1‑150 (MQ=255)
                                                                                                                                               atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTCTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa    >  1:1595666/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
TTATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524185‑524478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A