Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,334:1 +T 100% pseudogene (239/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,3341.T99.1% 1075.9 / 2.4 329pseudogene (239/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base T (152/173);  total (155/174)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 377 total aligned reads displayed.

TATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524186‑524478
                                                                                                                                                     |                                                                                                                                                
 atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑tt                                                                                                                                                >  2:2920678/1‑150 (MQ=255)
 atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑tt                                                                                                                                                <  2:4099829/150‑1 (MQ=255)
 atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑tt                                                                                                                                                >  1:2108371/1‑150 (MQ=255)
  tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttt                                                                                                                                               <  1:4726323/150‑1 (MQ=255)
  tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttt                                                                                                                                               <  2:2525934/150‑1 (MQ=255)
  tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttt                                                                                                                                               <  1:2568619/150‑1 (MQ=255)
     ataCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑tttttt                                                                                                                                            <  1:1846161/150‑1 (MQ=255)
      taCAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttt                                                                                                                                           <  2:5329479/150‑1 (MQ=255)
         aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttct                                                                                                                                        <  2:3463666/150‑4 (MQ=255)
         aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttct                                                                                                                                        >  1:1667362/1‑147 (MQ=255)
         aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttct                                                                                                                                        >  1:5025684/1‑147 (MQ=255)
          aaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctt                                                                                                                                       >  2:250001/1‑146 (MQ=255)
           aGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctta                                                                                                                                      >  2:3675432/1‑145 (MQ=255)
           aGATGAATCTATTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctta                                                                                                                                      <  1:935554/150‑6 (MQ=255)
            gATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAt                                                                                                                                      >  2:4652774/1‑150 (MQ=255)
            gATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAt                                                                                                                                      <  1:1807130/150‑1 (MQ=255)
               gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAtttt                                                                                                                                   >  1:1912244/1‑150 (MQ=255)
               gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAtttt                                                                                                                                   >  2:4852639/1‑150 (MQ=255)
                  tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTTGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTca                                                                                                                                <  2:4326771/150‑2 (MQ=255)
                   ctctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTccc                                                                                                                               <  2:2040781/150‑1 (MQ=255)
                   ctctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTccc                                                                                                                               >  2:3345451/1‑150 (MQ=255)
                      ttGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTaa                                                                                                                            >  1:1478882/1‑150 (MQ=255)
                         taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTACTTTTTTTCTTATTTTTCCCTAACaa                                                                                                                         >  1:4152628/1‑150 (MQ=255)
                           agtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAat                                                                                                                       >  1:2214674/1‑150 (MQ=255)
                           agtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAat                                                                                                                       <  2:1500254/150‑1 (MQ=255)
                              aaaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgat                                                                                                                    >  2:341536/1‑150 (MQ=255)
                               aaCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatg                                                                                                                   <  2:2302110/150‑1 (MQ=255)
                                  aTTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgatg                                                                                                                >  2:1981520/1‑150 (MQ=255)
                                   ttACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATgg                                                                                                               <  2:3895544/150‑1 (MQ=255)
                                     aCATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATgggg                                                                                                             >  1:3061703/1‑150 (MQ=255)
                                      cATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATggggg                                                                                                            >  2:659529/1‑150 (MQ=255)
                                       aTAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                           >  1:1028455/1‑150 (MQ=255)
                                       aTAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGt                                                                                                           >  1:2028280/1‑150 (MQ=255)
                                          aaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAgg                                                                                                        >  2:27718/1‑150 (MQ=255)
                                            cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                      >  2:139962/1‑150 (MQ=255)
                                            cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                      >  1:4648565/1‑150 (MQ=255)
                                                    cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                              >  2:3525035/1‑150 (MQ=255)
                                                    cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                              >  1:5176263/1‑150 (MQ=255)
                                                    cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                              <  2:4203010/150‑1 (MQ=255)
                                                     aaTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAAc                                                                                             <  1:316000/150‑1 (MQ=255)
                                                     aaTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAAc                                                                                             >  2:1864529/1‑150 (MQ=255)
                                                         ccAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTg                                                                                         <  1:4358143/150‑1 (MQ=255)
                                                           aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  1:451181/1‑150 (MQ=255)
                                                           aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  2:2986484/1‑150 (MQ=255)
                                                           aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  2:1207306/1‑150 (MQ=255)
                                                           aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  1:2907566/1‑150 (MQ=255)
                                                           aGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCAATAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGAc                                                                                       >  2:4766507/1‑150 (MQ=255)
                                                               aaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg                                                                                   <  1:3972646/150‑1 (MQ=255)
                                                               aaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg                                                                                   >  2:5100503/1‑150 (MQ=255)
                                                                 tgatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAg                                                                                 <  1:3497283/150‑1 (MQ=255)
                                                                  gatgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAgg                                                                                >  2:1031273/1‑150 (MQ=255)
                                                                  gatgGAGTTATTACATGTCTCCCAGATTTTTTTCCGCACGTAGCGTTGTATATATCAATATACACTAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTTACAAATGATGTTGGGGGTAGGTTTAAGAAAACATTGACAGTGAgg                                                                                <  2:907460/150‑1 (MQ=255)
                                                                    tgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGc                                                                              <  2:5187336/150‑1 (MQ=255)
                                                                        gTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAgg                                                                          <  2:1105229/150‑1 (MQ=255)
                                                                                 tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACACATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 >  2:137039/1‑150 (MQ=255)
                                                                                 tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAATATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 <  1:1867273/150‑1 (MQ=255)
                                                                                 tGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 <  2:4019137/150‑1 (MQ=255)
                                                                                 tGGCTCCCAGATATTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                 <  1:2689152/150‑1 (MQ=255)
                                                                                    cTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCg                                                              <  1:3243779/150‑1 (MQ=255)
                                                                                      cccAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaa                                                            >  1:4932369/1‑150 (MQ=255)
                                                                                       ccAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaaa                                                           <  1:2477240/150‑1 (MQ=255)
                                                                                         aGATTTTTTTCCGCACGTAGCGGTGGATTTATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAAt                                                         <  2:4718842/150‑1 (MQ=255)
                                                                                              tttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                    >  2:447075/1‑150 (MQ=255)
                                                                                              tttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                    <  1:3629564/150‑1 (MQ=255)
                                                                                              tttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                    >  2:5038241/1‑150 (MQ=255)
                                                                                               ttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATc                                                   <  1:2648234/150‑1 (MQ=255)
                                                                                                   ccGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAg                                               <  1:3025097/150‑1 (MQ=255)
                                                                                                      cACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAAt                                            <  1:2996042/150‑1 (MQ=255)
                                                                                                       aagTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCATAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATg                                           >  1:3791853/3‑150 (MQ=255)
                                                                                                         gTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGAt                                         <  2:1010114/150‑1 (MQ=255)
                                                                                                           aGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATcc                                       <  1:4876143/150‑1 (MQ=255)
                                                                                                            gCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCa                                      <  1:3837515/150‑1 (MQ=255)
                                                                                                             cGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAg                                     <  2:1087286/150‑1 (MQ=255)
                                                                                                               gttgATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGAt                                   <  2:3151429/147‑1 (MQ=255)
                                                                                                                tGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATa                                  >  2:491802/1‑150 (MQ=255)
                                                                                                                tGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATa                                  >  2:4596479/1‑150 (MQ=255)
                                                                                                                 ggATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATTGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAc                                 <  2:1902523/150‑1 (MQ=255)
                                                                                                                       atCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaat                           >  2:3762998/1‑149 (MQ=255)
                                                                                                                       atCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaa                           >  2:1765616/1‑150 (MQ=255)
                                                                                                                       atCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaa                           >  1:611694/1‑150 (MQ=255)
                                                                                                                         cAATATACACAAATGTAGAAGATTATTATTTTTTTTCTTATTTTTCCCTAAAAACTGATGATGGGGGTATGTTTAAGAAAAAATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaaat                         <  2:4536147/150‑2 (MQ=255)
                                                                                                                            tataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtgt                      >  1:5291615/1‑150 (MQ=255)
                                                                                                                            tataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtgt                      <  2:709804/150‑1 (MQ=255)
                                                                                                                             ataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGtgtg                     >  1:1177211/1‑150 (MQ=255)
                                                                                                                             ataCACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGtgtg                     >  1:1300884/1‑150 (MQ=255)
                                                                                                                                 acaAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGgtgt                 >  2:1554130/1‑150 (MQ=255)
                                                                                                                                  caAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTa                >  1:3345180/1‑150 (MQ=255)
                                                                                                                                  caAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTa                >  1:369671/1‑150 (MQ=255)
                                                                                                                                   aaaTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTaa               <  1:2518686/150‑1 (MQ=255)
                                                                                                                                     aTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat             <  1:883282/150‑1 (MQ=255)
                                                                                                                                     aTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat             >  2:1916411/1‑150 (MQ=255)
                                                                                                                                     aTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat             <  2:1691200/150‑1 (MQ=255)
                                                                                                                                       gTATAAGATGATTATTTTTTTTATTATTTTTCCCTAACAAATGATGATGGGTGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAATTGTGGTGTAAatat           <  1:2334579/150‑1 (MQ=255)
                                                                                                                                        tAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAGAAAAAGTGTGGTGTAAATATg          >  2:168096/1‑150 (MQ=255)
                                                                                                                                        tAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGAATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATg          >  2:3597463/1‑150 (MQ=255)
                                                                                                                                          gaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGt        <  2:797360/150‑1 (MQ=255)
                                                                                                                                            agaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTaa      >  1:4319081/1‑150 (MQ=255)
                                                                                                                                              atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa    >  1:2787351/1‑150 (MQ=255)
                                                                                                                                               tgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt   <  2:212653/150‑1 (MQ=255)
                                                                                                                                               tgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt   <  1:163588/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATA  >  CP009273/524186‑524478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A