Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,334:1 +T 100% pseudogene (239/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,3341.T90.7% 469.7 / 43.6 140pseudogene (239/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base . (4/9);  new base T (65/62);  total (69/71)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 153 total aligned reads displayed.

TATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATAC  >  CP009273/524186‑524479
                                                                                                                                                      |                                                                                                                                                 
tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t                                                                                                                                                  <  2:2452477/150‑1 (MQ=255)
tatttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑t                                                                                                                                                  <  2:3508566/150‑1 (MQ=255)
 atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑tt                                                                                                                                                 <  2:153212/150‑1 (MQ=255)
 atttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGatta‑tt                                                                                                                                                 >  2:1566294/1‑150 (MQ=255)
  tttatACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttt                                                                                                                                                >  1:3408052/1‑150 (MQ=255)
        cAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttc                                                                                                                                          >  1:1018642/1‑148 (MQ=255)
         aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttct                                                                                                                                         <  2:2918302/150‑4 (MQ=255)
         aaaGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttct                                                                                                                                         <  2:2109856/150‑4 (MQ=255)
           aGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑ttttttttctta                                                                                                                                       >  1:2453709/1‑145 (MQ=255)
            gATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAt                                                                                                                                       >  1:1544382/1‑150 (MQ=255)
               gAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAtttt                                                                                                                                    >  2:874387/1‑150 (MQ=255)
                aaTCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTAttttt                                                                                                                                   <  2:1859000/150‑1 (MQ=255)
                  tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTcc                                                                                                                                 <  2:3571130/150‑1 (MQ=255)
                  tctcTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTcc                                                                                                                                 <  2:3571269/150‑1 (MQ=255)
                    tctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCt                                                                                                                               <  2:3972274/150‑1 (MQ=255)
                     ctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTa                                                                                                                              <  2:2574752/150‑1 (MQ=255)
                     ctTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTa                                                                                                                              <  2:552399/150‑1 (MQ=255)
                     attGTAATTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTa                                                                                                                              <  2:3621690/149‑1 (MQ=255)
                       tGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAAc                                                                                                                            >  2:1484110/1‑150 (MQ=255)
                        gtaagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGATCCCAGA‑TTTTTTTCCTCACGTAGCGGTGGATATATCAAAAAAAACAAATGAAGAATATGATTA‑TTTTTTACTTATTTTTACCTAaaaa                                                                                                                          >  1:1449142/1‑150 (MQ=255)
                         taagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACaa                                                                                                                          <  2:2415412/150‑1 (MQ=255)
                          aagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACaaa                                                                                                                         <  2:3024146/150‑1 (MQ=255)
                          aagtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACaaa                                                                                                                         <  2:3023889/150‑1 (MQ=255)
                           agtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTACCTAACAAat                                                                                                                        >  2:3468223/1‑150 (MQ=255)
                            gtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatg                                                                                                                       <  2:3151103/150‑1 (MQ=255)
                            gtaaACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatg                                                                                                                       <  2:3150695/150‑1 (MQ=255)
                                aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatga                                                                                                                   <  1:2941203/150‑1 (MQ=255)
                                aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatga                                                                                                                   <  1:2347219/150‑1 (MQ=255)
                                aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                  <  1:53251/150‑1 (MQ=255)
                                aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                  <  1:503130/150‑1 (MQ=255)
                                 cATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                  >  2:1165522/1‑150 (MQ=255)
                                 cATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                  <  2:3374592/150‑1 (MQ=255)
                                 cATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgat                                                                                                                  <  1:3738157/150‑1 (MQ=255)
                                  aTTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAatgatgatg                                                                                                                 <  1:2594500/150‑1 (MQ=255)
                                   ttACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGAggg                                                                                                                >  2:2893902/1‑150 (MQ=255)
                                   ttACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATgg                                                                                                                <  2:3847806/150‑1 (MQ=255)
                                    tACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATggg                                                                                                               <  2:270147/150‑1 (MQ=255)
                                     aCATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATgggg                                                                                                              <  1:3635149/150‑1 (MQ=255)
                                     aCATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATgggg                                                                                                              <  1:3635200/150‑1 (MQ=255)
                                     aCATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATgggg                                                                                                              >  1:960318/1‑150 (MQ=255)
                                        tAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACACATGATGAGGGGGGTa                                                                                                           >  1:4016724/1‑150 (MQ=255)
                                         aaaCTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAg                                                                                                          >  2:1011741/1‑150 (MQ=255)
                                            cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGtt                                                                                                       >  2:2409301/1‑150 (MQ=255)
                                            cTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGttt                                                                                                      >  2:83994/1‑150 (MQ=255)
                                             tataTGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGttt                                                                                                      >  2:772421/1‑150 (MQ=255)
                                              atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTa                                                                                                     <  1:86732/150‑1 (MQ=255)
                                              atatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTa                                                                                                     <  1:86733/150‑1 (MQ=255)
                                               tatGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTaa                                                                                                    >  1:2197913/1‑150 (MQ=255)
                                                 tGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGa                                                                                                  >  2:3826893/1‑150 (MQ=255)
                                                  ggCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaa                                                                                                 >  2:848742/1‑150 (MQ=255)
                                                    cAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGaaaa                                                                                               >  1:2806477/1‑150 (MQ=255)
                                                     aaTACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTCACAAAGGATGATGGGGGTAGGTTTAAGAAAAc                                                                                              >  1:741309/1‑150 (MQ=255)
                                                       tACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACAt                                                                                            <  1:2064692/150‑1 (MQ=255)
                                                          cAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGTATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa                                                                                         <  1:348366/150‑1 (MQ=255)
                                                             aaaaTGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAg                                                                                      >  1:1729871/1‑150 (MQ=255)
                                                             aaaaTGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAg                                                                                      >  1:1841703/1‑150 (MQ=255)
                                                             aaaaTGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAg                                                                                      >  2:3544360/1‑150 (MQ=255)
                                                             aaaaTGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAg                                                                                      >  2:3520678/1‑150 (MQ=255)
                                                                atgatgGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGa                                                                                   >  2:454706/1‑150 (MQ=255)
                                                                  gatgGAGTTTTTACATGGCTCCCAGATTTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAgg                                                                                 >  2:838974/1‑150 (MQ=255)
                                                                     ggAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGcc                                                                              <  2:2549812/150‑1 (MQ=255)
                                                                     ggAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGcc                                                                              <  2:2549961/150‑1 (MQ=255)
                                                                      gAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCa                                                                             <  1:436368/150‑1 (MQ=255)
                                                                          ttttACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGa                                                                         >  2:4356621/1‑150 (MQ=255)
                                                                             tACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACa                                                                      <  1:2409301/150‑1 (MQ=255)
                                                                                 tGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                  >  1:4317003/1‑150 (MQ=255)
                                                                                 tGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTg                                                                  >  1:4316997/1‑150 (MQ=255)
                                                                                      cccAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaa                                                             >  2:729716/1‑150 (MQ=255)
                                                                                       ccAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGaaa                                                            >  2:837702/1‑150 (MQ=255)
                                                                                         aGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGACCAAGTGGCGAAAAt                                                          >  2:3656080/1‑150 (MQ=255)
                                                                                         aGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAAt                                                          <  2:713696/150‑1 (MQ=255)
                                                                                              ttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTa                                                      >  1:2412006/1‑150 (MQ=255)
                                                                                              ttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                     <  2:3728895/150‑1 (MQ=255)
                                                                                              ttttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                     <  2:3727769/150‑1 (MQ=255)
                                                                                               tttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                     <  2:53060/150‑1 (MQ=255)
                                                                                               tttttCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTAt                                                     <  2:52760/150‑1 (MQ=255)
                                                                                                        aCGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATg                                            <  1:3992732/150‑1 (MQ=255)
                                                                                                         cGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGa                                           >  2:2761448/1‑150 (MQ=255)
                                                                                                                    atatatAAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaa                                <  2:3408052/150‑1 (MQ=255)
                                                                                                                      atatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaa                              >  1:3460928/1‑150 (MQ=255)
                                                                                                                       tatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGTACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa                             >  2:2738612/1‑150 (MQ=255)
                                                                                                                       tatCAATATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa                             >  2:492294/1‑150 (MQ=255)
                                                                                                                           aaTATACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaaagtg                         >  2:2268369/1‑147 (MQ=255)
                                                                                                                            atatACACAAATGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtg                        <  1:3032850/150‑1 (MQ=255)
                                                                                                                                      aTGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAat              >  1:3364839/1‑150 (MQ=255)
                                                                                                                                       tGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAata             >  2:4150876/1‑150 (MQ=255)
                                                                                                                                       tGTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAata             >  1:627448/1‑150 (MQ=255)
                                                                                                                                        gTAGAAGATGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAatat            >  2:3480161/1‑150 (MQ=255)
                                                                                                                                          agaagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATgg          <  1:4028980/150‑1 (MQ=255)
                                                                                                                                            aagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTa        >  1:735530/1‑150 (MQ=255)
                                                                                                                                            aagaTGATTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTa        <  1:449189/150‑1 (MQ=255)
                                                                                                                                             agaTGACTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGACCAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACACAAAAAGTGTGGTGTAAATATGGTaa       >  2:3465150/1‑150 (MQ=255)
                                                                                                                                               atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa     >  2:4118169/1‑150 (MQ=255)
                                                                                                                                               atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa     >  2:4309502/1‑150 (MQ=255)
                                                                                                                                               atgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGa     >  2:990309/1‑150 (MQ=255)
                                                                                                                                                tgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt    <  1:2028370/150‑1 (MQ=255)
                                                                                                                                                tgatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGAt    <  1:1683955/150‑1 (MQ=255)
                                                                                                                                                 gatTATTTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATa   <  2:1054272/150‑1 (MQ=255)
                                                                                                                                                 gatTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATAc  <  2:641104/150‑1 (MQ=255)
                                                                                                                                                 gatTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATAc  <  2:641178/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
TATTTATACAAAGATGAATCTCTTGTAAGTAAACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGA‑TTTTTTTCCGCACGTAGCGGTGGATATATCAATATACACAAATGTAGAAGATGATTA‑TTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATAC  >  CP009273/524186‑524479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A